Abstract:
:Insulin peptide, acting through tyrosine kinase receptor pathways, contributes to nerve development or repair. In this work, we examined the direction, impact and repertoire of insulin signaling in vivo during peripheral nerve regeneration in rats. First, we demonstrated that insulin receptor is expressed on lumbar dorsal root ganglia neuronal perikarya using immunohistochemistry. Immunoblots and polymerase chain reactions confirmed the presence of both alpha and beta insulin receptor subunits in dorsal root ganglia. In vivo and in vitro assessment of dorsal root ganglion neurons showed preferential localization of insulin receptor to perikaryal sites. In vivo, intrathecal delivery of fluorescein isothiocyanate-labeled insulin identified localization around dorsal root ganglia neurons. The direction and impact of potential insulin signaling was evaluated by concurrently delivering insulin or carrier over a 2 week period using mini-osmotic pumps, either intrathecally, near nerve, or with both deliveries, following a selective sural nerve crush injury. Only intrathecal insulin increased the number and maturity of regenerating sensory sural nerve axons distal to the crush site. As well, only intrathecal insulin rescued retrograde loss of sural axons after crush. In a separate experiment, insulin also rescued retrograde loss and atrophy of deep peroneal, largely motor, axons post-injury. Intrathecal insulin increased the expression of calcitonin-gene-related peptide in regenerating sprouts, increased the number of visualized regenerating fiber clusters, and reduced downregulation of calcitonin-gene-related peptide in dorsal root ganglia neurons. Insulin delivered intrathecally does not appear to influence expression of insulin-like growth factor-1 at dorsal root ganglion neurons or near peripheral nerve injury, but was associated with upregulation of insulin receptor alpha subunit in dorsal root ganglia. Intrathecal insulin delivery was associated with greater recovery of thermal sensation and longer distances to stimulus response with the pinch test following sural nerve crush. Insulin signaling at neuron perikarya can drive distal sensory axon regrowth, rescue retrograde alterations of axons and alter axon peptide expression. Moreover, such actions are associated with upregulation of its own receptor.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Toth C,Brussee V,Martinez JA,McDonald D,Cunningham FA,Zochodne DWdoi
10.1016/j.neuroscience.2005.11.065keywords:
subject
Has Abstractpub_date
2006-05-12 00:00:00pages
429-49issue
2eissn
0306-4522issn
1873-7544pii
S0306-4522(05)01325-4journal_volume
139pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Synaptic plasticity involves remodeling of extracellular matrix. This is mediated, in part, by enzymes of the matrix metalloproteinase (MMP) family, in particular by gelatinase MMP-9. Accordingly, there is a need of developing methods to visualize gelatinolytic activity at the level of individual synapses, especially ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.05.045
更新日期:2009-01-12 00:00:00
abstract::The amygdaloid complex plays an essential role in auditory fear conditioning of the Pavlovian type. The available evidence suggests that the lateral nucleus is the input station of the amygdala for auditory conditioned stimuli, whereas the central medial nucleus is the output for conditioned fear responses. However, t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00272-k
更新日期:1995-11-01 00:00:00
abstract::Attentional automatic processes and cerebral activity may differ between individuals with different weight statuses in the presence of food stimuli (e.g. odors, pictures). In the present study, we used an implicit olfactory priming paradigm to test the influence of non-attentively perceived food odors on the cerebral ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2021.01.015
更新日期:2021-01-19 00:00:00
abstract::The periaqueductal gray (PAG) is part of a descending pain modulatory system that, when activated, produces widespread and profound antinociception. Microinjection of either opioids or cannabinoids into the PAG elicits antinociception. Moreover, microinjection of the cannabinoid 1 (CB1) receptor agonist HU-210 into th...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.03.038
更新日期:2012-06-28 00:00:00
abstract::Biochemical and immunocytochemical analyses were performed to resolve how glutamate and glutamine are handled in rat cerebellar cortex in acute hypoosmotic stress. Rats were subjected to a 15-20% reduction in plasma osmolality by intraperitoneal injection of distilled water and then perfusion fixed after 4 or 8 h surv...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00003-6
更新日期:1996-06-01 00:00:00
abstract::Rearing rats in isolation has been shown to produce behavioral and neurochemical alterations similar to those observed in psychoses such as schizophrenia. Also, a dysregulation in both the endocannabinoid and dopaminergic systems has been implicated in schizophrenia. The aim of this study was to determine if there are...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.10.043
更新日期:2008-03-03 00:00:00
abstract::The innervation of the suprachiasmatic nucleus by serotoninergic fibers has been studied in rats from the 22nd embryonic until the 21st postnatal day. The serotoninergic fibers were detected with the pre-embedding immunocytochemical technique at the electron-microscopic level using antibodies to serotonin. Serotonin-i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90163-5
更新日期:1994-01-01 00:00:00
abstract::Many of the different morphological types of interneurons in mammalian neocortex are presumed to be inhibitory, but to date, conclusive functional data have been lacking. Using paired intracellular recordings in slices of adult rat somatosensory cortex, we present a sparsely spiny, burst firing interneuron that elicit...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00020-j
更新日期:1995-04-01 00:00:00
abstract::Arousal enhances the readiness to process sensory information and respond to it. Rapid increment of arousal, referred to as arousal reaction or startle, increases the level of attention and the chance of survival. Arousal reaction is known to originate from the brainstem ascending reticular activating system and to mo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.05.060
更新日期:2014-08-22 00:00:00
abstract::Brainstem networks generating the respiratory rhythm in lampreys are still not fully characterized. In this study, we described the patterns of respiratory activities and we identified the general location of underlying neural networks. In a semi-intact preparation including the brain and gills, rhythmic discharges we...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.05.023
更新日期:2007-08-10 00:00:00
abstract::Although extensively investigated in socio-cognitive neuroscience, empathy is difficult to study. The first difficulty originates in its multifaceted nature. According to the multidimensional model, empathy combines emotional, automatic (simulation), cognitive (mentalizing) and regulatory (executive functions) process...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.02.024
更新日期:2014-05-16 00:00:00
abstract::TREK-1 is a member of the two-pore-domain potassium channel family which is expressed predominantly in the CNS. Using an anti-peptide polyclonal antiserum, we have determined the distribution of TREK-1 in the brain and spinal cord of adult rats. Specificity of the antiserum was tested using a TREK-1-transfected cell l...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00030-6
更新日期:2001-01-01 00:00:00
abstract::Regulation of adult hippocampal neurogenesis in mice responds to behavioral stimuli, including physical activity (RUN) and the exposure to enriched environments (ENR). If studied after days or weeks, these stimuli and the pathological stimulus of kainic acid-induced seizures (KA) show differential effects on different...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.04.023
更新日期:2008-06-23 00:00:00
abstract::Many behaviorally relevant sounds, including language, are composed of brief, rapid, repetitive acoustic features. Recent studies suggest that abnormalities in producing and understanding spoken language are correlated with abnormal neural responsiveness to such auditory stimuli at higher auditory levels [Tallal et al...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00240-3
更新日期:2002-01-01 00:00:00
abstract::Models of the saccadic system differ in several respects including the signals fed back to their comparators, as well as the location and identity of the units that could serve as comparators. Some models place the comparator in the superior colliculus while others assign this role to the reticular formation. To test ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.07.016
更新日期:2006-11-17 00:00:00
abstract::Niemann-Pick Type C1 (NPC1) is an autosomal recessive disorder characterized by the accumulation of cholesterol and glycosphingolipids. Combination-treatment utilizing cyclodextrin, allopregnanolone and miglustat (CYCLO/ALLO/miglustat) can ameliorate NPC1 disease in a mutant mouse model. The present study was designed...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.001
更新日期:2013-11-12 00:00:00
abstract::A quantitative electron-microscopic analysis has been conducted on the neurons within the gustatory zone of the nucleus of the solitary tract of the hamster. The most common group of neurons within the gustatory zone contains both large (X1) and small (X3) members that possess deeply invaginated nuclear profiles. Thes...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90198-4
更新日期:1988-12-01 00:00:00
abstract::γ oscillations (30-120 Hz) are generated intrinsically within local networks in the mammalian olfactory bulb (OB). The OB directly receives peripheral input from olfactory sensory neurons (OSNs) that can respond to nasal airflow, and centrifugal input from neuromodulatory systems whose activities are affected by the b...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.12.011
更新日期:2019-02-01 00:00:00
abstract::Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.12.073
更新日期:2007-05-25 00:00:00
abstract::The behavioral and motivational changes that result from use of abused substances depend upon activation of neuronal populations in the reward centers of the brain, located primarily in the corpus striatum in primates. To gain insight into the cellular mechanisms through which abused drugs reinforce behavior in the pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.06.002
更新日期:2009-09-29 00:00:00
abstract::The pathological significance of intracellular Abeta accumulation in vivo is not yet fully understood. To address this, we have studied transgenic rats expressing Alzheimer's-related transgenes that accumulate Abeta intraneuronally in the cerebral and hippocampal cortices but do not develop extracellular amyloid plaqu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.07.036
更新日期:2004-01-01 00:00:00
abstract::Rats were given bilateral lesions of the motor cortex on the tenth day of life, and then received a daily subcutaneously injection of either basic fibroblast growth factor (FGF-2) or vehicle for 7 consecutive days. In adulthood, they were trained and assessed on a skilled forelimb reaching task. Although all lesion gr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.04.006
更新日期:2005-01-01 00:00:00
abstract::Calcium permeabilities were examined in large cerebellar neurons maintained in culture, and morphologically identified as Purkinje cells. When cells were supplied with a Dulbecco Minimum Eagle's Medium with 10% horse serum added (5-10 days), somatic recordings revealed complex spikes and these were shown to be generat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90155-3
更新日期:1989-01-01 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) produce widespread and complex effects on neocortex excitability. We studied how heteromeric nAChRs regulate inhibitory post-synaptic currents (IPSCs), in fast-spiking (FS) layer V neurons of the mouse frontal area 2 (Fr2). In the presence of blockers of ionotropic glutamate ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.10.045
更新日期:2017-01-06 00:00:00
abstract::Several lines of research have implicated glutathione (GSH) in schizophrenia. For instance, GSH deficiency has been reported in the prefrontal cortex of schizophrenics in vivo. Further, in rats postnatal GSH-deficiency combined with hyperdopaminergia led to cognitive impairments in the adult. In the present report we ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.01.059
更新日期:2005-01-01 00:00:00
abstract::Interleukin-1 (IL-1) plays a crucial role in stress responses and its mRNA is induced in the brain by stress load; however, the precise role of IL-1 in higher brain functions and their abnormalities is largely unknown. Here, we report that IL-1 receptor antagonist (IL-1Ra) knockout (KO) mice, which lack IL-1Ra molecul...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.031
更新日期:2011-09-29 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) was investigated in the present study to examine whether it could affect the activation status of microglia under microenvironment of spinal cord injury and provide a potential therapeutic treatment for spinal cord injury. We established mouse spinal cord hemisection model...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.01.047
更新日期:2013-05-15 00:00:00
abstract::GABA(B) receptors are G-protein-coupled receptors mediating the slow onset and prolonged synaptic actions of GABA in the CNS. The recent cloning of two genes, GABA(B1) and GABA(B2), has revealed a novel requirement for GABA(B) receptor signalling. Studies have demonstrated that the two receptor subunits associate as a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00296-2
更新日期:2001-01-01 00:00:00
abstract::The aim of the present study was to examine the fine structural changes in the superior cervical ganglion of adult rats following baclofen treatment. On the addition of this GABAB receptor agonist there was a transient increase in the number of coated vesicles and pits in the dendrites of principal sympathetic neurons...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90365-b
更新日期:1990-01-01 00:00:00
abstract::In the glomerular layer of the rat main olfactory bulb, we previously reported three chemically defined interneuron groups: GABA-like immunoreactive, calretinin-immunoreactive and Calbindin-D28k-immunoreactive groups [Kosaka K. et al. (1995) Neurosci. Res. 23, 73-88]. In the present study, we analysed the structural f...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00308-9
更新日期:1997-02-01 00:00:00