Abstract:
:Stable carbon isotope fractionation during dichloromethane (DCM) degradation by methylotrophic bacteria was investigated under aerobic and nitrate-reducing conditions. The strains studied comprise several Hyphomicrobium strains, Methylobacterium, Methylopila, Methylophilus and Methylorhabdus spp. that are considered to degrade DCM by a glutathione (GSH)-dependent dehalogenase enzyme system in the initial step. The stable carbon isotope fractionation factors (alphaC) of the strains varied under aerobic conditions between 1.043 and 1.071 and under nitrate-reducing conditions between 1.048 and 1.065. Comparison of isotope fractionation under aerobic and nitrate-reducing conditions by individual strains revealed only minor to no differences. The variability in isotope fractionation among strains was found to be related to the polymorphism of the functional genes encoding the DCM dehalogenase.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Nikolausz M,Nijenhuis I,Ziller K,Richnow HH,Kästner Mdoi
10.1111/j.1462-2920.2005.00878.xkeywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
156-64issue
1eissn
1462-2912issn
1462-2920pii
EMI878journal_volume
8pub_type
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