18F-FEAU as a radiotracer for herpes simplex virus thymidine kinase gene expression: in-vitro comparison with other PET tracers.

Abstract:

OBJECTIVE:The herpes simplex virus thymidine kinase (HSVtk) gene has frequently been applied as a reporter gene for monitoring transgene expression in animal models. In clinical gene therapy protocols, however, extremely low expression levels of the transferred gene are generally observed. Consequently, sensitive and selective radiotracers for imaging are required. This study describes the in-vitro evaluation of 2'-[18F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil (18F-FEAU) as a candidate tracer for HSVtk imaging with positron emission tomography (PET). METHODS:In cellular accumulation experiments, the potential of 18F-FEAU as a PET tracer for HSVtk was compared to the known acyclic guanosine derivatives 9-[(3-[18F]fluoro-1-hydroxy-2-propoxy)methyl]guanine (18F-FHPG) and 9-[4-[18F]fluoro-3-(hydroxymethyl)butyl]guanine (18F-FHBG), and the thymidine derivatives 3'-deoxy-3'-[18F]fluorothymidine (18F-FLT), 2'-deoxy-2'-[18F]fluoro-5-methyl-1-beta-D-arabinofuranosyluracil (18F-FMAU) and 2'-deoxy-2'-[18F]fluoro-5-iodo-1-beta-D-arabinofuranosyluracil (18F-FIAU). For this purpose, C6 control cells and HSVtk-expressing C6tk cells were incubated with the different tracers for various periods of time and cellular uptake and initial uptake rates were analysed. The initial rate of tracer uptake was determined from the slope of the plot of tracer uptake versus incubation time. RESULTS:After 2 h of tracer incubation, the C6tk/C6 accumulation ratio was 1.6 for 18F-FLT, 2.4 for F-FMAU, 5.5 for 18F-FHPG, 10.3 for 18F-FIAU, 40.8 for 18F-FHBG and 84.5 for 18F-FEAU. The initial tracer uptake rate in C6tk cells was in the order FLT>FMAU>FEAU>FIAU>FHBG>FHPG, whereas the initial tracer uptake rate in C6 control cells was FLT>FMAU>FIAU>FEAU approximately = FHBG approximately = FHPG. The highest HSVtk specific uptake was observed for FEAU. CONCLUSION:This study indicates that the high uptake rate of FEAU together with its high selectivity make this tracer an excellent candidate as a PET tracer for HSVtk gene expression.

journal_name

Nucl Med Commun

authors

Buursma AR,Rutgers V,Hospers GA,Mulder NH,Vaalburg W,de Vries EF

doi

10.1097/01.mnm.0000186609.12895.20

keywords:

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

25-30

issue

1

eissn

0143-3636

issn

1473-5628

pii

00006231-200601000-00005

journal_volume

27

pub_type

杂志文章
  • A 5-year longitudinal evaluation in patients with mild cognitive impairment by 11C-PIB PET/CT: a visual analysis.

    abstract:OBJECTIVE:The aim of this study was to evaluate the cerebral amyloid distribution in patients with mild cognitive impairment (MCI), assessed by carbon-11-Pittsburgh compound B (C-PIB) PET/CT, after 5 years of follow-up. PATIENTS AND METHODS:Ten amnestic MCI (A-MCI) and four nonamnestic (NA-MCI) patients were studied b...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000001004

    authors: Jiménez-Bonilla JF,Quirce R,De Arcocha-Torres M,Martínez-Rodríguez I,Martínez-Amador N,Sánchez-Salmón A,De la Fuente-Gómez F,Rodríguez E,Sánchez-Juan P,Banzo I

    更新日期:2019-05-01 00:00:00

  • Evaluation of motion-correction methods for dual-gated cardiac positron emission tomography/computed tomography imaging.

    abstract:BACKGROUND:Dual gating is a method of dividing the data of a cardiac PET scan into smaller bins according to the respiratory motion and the ECG of the patient. It reduces the undesirable motion artefacts in images, but produces several images for interpretation and decreases the quality of single images. By using motio...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000000539

    authors: Klén R,Noponen T,Koikkalainen J,Lötjönen J,Thielemans K,Hoppela E,Sipilä H,Teräs M,Knuuti J

    更新日期:2016-09-01 00:00:00

  • Time course of thyroglobulin autoantibodies in patients with differentiated thyroid carcinoma after radioiodine therapy.

    abstract::The time course of thyroglobulin (Tg) was monitored in patients with differentiated thyroid carcinoma after ablative therapy (thyroidectomy + 131I-treatment) with particular respect to endogeneous antibodies (Tg-ABs). In 232 patients (196 without Tg-ABs, 36 with Tg-ABs at the time of treatment) a sensitivity of 88% (a...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-198405000-00003

    authors: Moser E,Braun S,Kirsch CM,Kleinhans E,Buell U

    更新日期:1984-05-01 00:00:00

  • Measurement of the extravascular concentration of renal agents following intravenous bolus injection.

    abstract::The extravascular concentration (Ce) of some renal agents, in relation to the simultaneous plasma concentration (Ci), was calculated as a function of time after intravenous injection. Four agents were studied: 51Cr-ethylenediamine tetraacetate (EDTA) (n = 11), 125I-hippuran (n = 11), 99Tcm-dimercaptosuccinic acid (DMS...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199402000-00002

    authors: Peters AM,Brown H,Cosgriff P

    更新日期:1994-02-01 00:00:00

  • Evaluation of 90Y phosphate particles as a possible radiation synoviorthesis agent.

    abstract:BACKGROUND:90Y is one of the radioisotopes used extensively for therapy due to its favourable nuclear characteristics. Particles and colloids incorporating 90Y are being used for radiation synovectomy, especially in European countries. METHODS:In our present work, 90Y phosphate particles were prepared and evaluated fo...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-200505000-00011

    authors: Pandey U,Bapat KN,Samuel G,Sarma HD,Chaudhari PR,Dhami PS,Kannan R,Venkatesh M

    更新日期:2005-05-01 00:00:00

  • Z-transform method for deconvolution as applied to the renogram.

    abstract::A method of deconvolution analysis involving the use of the z-transform has been developed. When compared to the matrix method, it is shown, under certain circumstances, to be identical. In order to study the validity of the method, simple mathematical models have been constructed. The curves obtained with the compute...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:

    authors: Bererhi H

    更新日期:1995-03-01 00:00:00

  • Image-derived input function in PET brain studies: blood-based methods are resistant to motion artifacts.

    abstract:BACKGROUND:Image-derived input function (IDIF) from carotid arteries is an elegant alternative to full arterial blood sampling for brain PET studies. However, a recent study using blood-free IDIFs found that this method is particularly vulnerable to patient motion. The present study used both simulated and clinical [11...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0b013e328356185c

    authors: Zanotti-Fregonara P,Liow JS,Comtat C,Zoghbi SS,Zhang Y,Pike VW,Fujita M,Innis RB

    更新日期:2012-09-01 00:00:00

  • Misdiagnoses of 11C-choline combined with 18F-FDG PET imaging in brain tumours.

    abstract:BACKGROUND AND OBJECTIVE:The widely applied F-FDG is known for its disadvantage in brain tumour PET imaging because of its high background uptake. C-choline can achieve high contrast of brain tumour imaging and was expected to have higher sensitivity and specificity. We analysed the misdiagnoses in C-choline PET imagin...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0b013e3282f4a21e

    authors: Huang Z,Zuo C,Guan Y,Zhang Z,Liu P,Xue F,Lin X

    更新日期:2008-04-01 00:00:00

  • Preoperative 18F-FDG PET/computed tomography predicts survival following resection for colorectal liver metastases.

    abstract:OBJECTIVE:The liver is the most frequent metastatic site from colorectal cancer and about 20% of these patients are treated by surgical resection. However, the 5-year disease-free survival (DFS) following resection is only about 25% and 5-year overall survival (OS) about 38%. The aim of the study was to evaluate the ab...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000001235

    authors: Grut H,Stern NM,Dueland S,Labori KJ,Dormagen JB,Schulz A

    更新日期:2020-09-01 00:00:00

  • Audit of nuclear medicine scientific and technical standards.

    abstract::The British Nuclear Medicine Society has developed a process for the service-specific organizational audit of nuclear medicine departments. This process identified the need for a scheme suitable for the audit of the scientific and technical standards of a department providing such a service. This document has evolved ...

    journal_title:Nuclear medicine communications

    pub_type: 指南,杂志文章,实务指引

    doi:10.1097/01.mnm.0000135041.46837.ca

    authors: Jarritt PH,Perkins AC,Woods SD,British Nuclear Medicine Society.

    更新日期:2004-08-01 00:00:00

  • Intraperitoneal 131I- and 111In-791T/36 monoclonal antibody in recurrent ovarian cancer: imaging and biodistribution.

    abstract::An examination of the biokinetics and biodistribution of i.p. administered 131I- or 111In-labelled 791T/36 antibody (1 mg) has been carried out in five patients with stage III/IV ovarian cancer. Blood kinetics and urinary excretion of the radiolabels were assayed. Scintigraphy was performed immediately following admin...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-198908010-00006

    authors: Perkins AC,Pimm MV,Gie C,Marksman RA,Symonds EM,Baldwin RW

    更新日期:1989-08-01 00:00:00

  • Relationship between various parameters derived from 123I-labelled beta-methyl-branched fatty acid whole-body scintigraphy and left ventricular ejection fraction in patients with ischaemic heart disease.

    abstract::Heart uptake and clearance of 123I-15-(p-iodophenyl)-3(R,S)-methyl- pentadecanoic acid (BMIPP) by whole-body scintigraphy in relation to left ventricular ejection fraction (LVEF) was evaluated in 34 patients with ischaemic heart disease. At 45 and 210 min after injection, anterior whole-body images were obtained. In e...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章

    doi:10.1097/00006231-199409000-00004

    authors: Matsunari I,Saga T,Taki J,Akashi Y,Wakasugi T,Hirai JI,Aoyama T,Matoba M,Nishikawa T,Ichiyanagi K

    更新日期:1994-09-01 00:00:00

  • Pharmacokinetics, biodistribution and dosimetry of 99Tcm(V)DMSA in humans with squamous cell carcinoma.

    abstract::Technetium-99m (99Tcm)(V) dimercaptosuccinic acid (DMSA) is a new tumour imaging agent which has been used to evaluate squamous carcinoma (SCC) of the head and neck. This study evaluated the pharmacokinetics and biodistribution of 99Tcm(V)DMSA in patients with SCC and calculated the bone mass of a New Zealand White (N...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199005000-00002

    authors: Watkinson JC,Allen S,Lazarus CR,Sinclair J,Blake GM,Clarke SE

    更新日期:1990-05-01 00:00:00

  • 99mTcN complexes of tert-butyl dithiocarbamate and methoxyisobutyl dithiocarbamate as myocardial and brain imaging agents.

    abstract:OBJECTIVE:Two ligands viz. tert-butyl dithiocarbamate (TBDTC) and methoxyisobutyl dithiocarbamate (MIBDTC), which are analogous to the tert-butyl isonitrile (TBI) and sestamibi (MIBI) ligands, were synthesized and labelled with the [99mTcN] core to evaluate their potential as myocardial agents. As these complexes have ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/01.mnm.0000183793.51474.2f

    authors: Mathur A,Mallia MB,Subramanian S,Banerjee S,Kothari K,Dhotare B,Sarma HD,Venkatesh M

    更新日期:2005-11-01 00:00:00

  • Difference in appearance between prone and supine myocardial perfusion images obtained on a high-efficiency cadmium zinc telluride SPECT camera.

    abstract:OBJECTIVE:The recent introduction of high-efficiency solid-state gamma cameras for myocardial perfusion single photon emission computed tomography has enabled lower patient radiation dose, faster imaging, and improved image quality. However, artifacts still complicate interpretation. Prone imaging is a common maneuver ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000000476

    authors: Howard BA,Roy Choudhury K,Oldan JD,Pagnanelli RA,Borges-Neto S

    更新日期:2016-05-01 00:00:00

  • Assessment of the relationship between coronary flow rates and myocardial perfusion abnormality in patients with nonobstructive coronary artery disease: an observational study in cardiac syndrome X and coronary slow flow.

    abstract:OBJECTIVES:In this study, we evaluated and compared the level of myocardial ischaemia caused by cardiac syndrome X (CSX) and coronary slow flow (CSF) with single photon emission computed tomography myocardial perfusion imaging (SPECT-MPI), and determined if changes in the level of myocardial ischaemia exist in CSF and ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000001080

    authors: Özde C,Aktüre G,Aytekin S,Sayin AE,Ay EK,Coçkun G,Kaya A,Kayapinar O

    更新日期:2019-11-01 00:00:00

  • 99Tcm-HMPAO brain SPECT in the evaluation of prognosis after surgical resection of astrocytoma. Comparison with other noninvasive imaging techniques (CT, MRI and 201Tl SPECT).

    abstract::High-grade astrocytoma represents the most common primary malignant brain tumour in the adult, and is associated with high morbidity and mortality rates. The aim of this study was to investigate the prognostic value of 99Tcm-hexamethylpropyleneamine oxime (HMPAO) brain single photon emission computed tomography (SPECT...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199312000-00002

    authors: Rodrigues M,Fonseca AT,Salgado D,Vieira MR

    更新日期:1993-12-01 00:00:00

  • Biodistribution and radiation dosimetry of [18F]F-PEB in nonhuman primates.

    abstract:INTRODUCTION:The metabotropic glutamate receptor subtype 5 (mGluR5) is distributed throughout the central nervous system (CNS), and has been suggested to be a potential target for several CNS disorders suchas Parkinson's disease, pain, anxiety, depression, schizophrenia, and addiction. We report here on the rhesus monk...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0b013e3283060c72

    authors: Bélanger MJ,Krause SM,Ryan C,Sanabria-Bohorquez S,Li W,Hamill TG,Burns HD

    更新日期:2008-10-01 00:00:00

  • Simultaneous assessment of regional adrenergic activity and perfusion with 123I-MIBG and 201Tl in congestive heart failure.

    abstract::Simultaneous assessment of regional adrenergic activity and perfusion with 123I-meta-iodobenzylguanidine (123I-MIBG) and 201Tl in various organs was performed in 13 patients with congestive heart failure (CHF) and 13 subjects without heart failure. In order to reduce the crosstalk of 201Tl to the 123I energy window to...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199603000-00009

    authors: Seto H,Shimizu M,Nozawa T,Yoshida N,Inagaki S,Kageyama M,Kakishita M

    更新日期:1996-03-01 00:00:00

  • Optimal buffer choice of the radiosynthesis of (68)Ga-Dotatoc for clinical application.

    abstract:OBJECTIVES:(68)Ga-Dotatoc has become the PET radiopharmaceutical of choice for the diagnosis and treatment follow-up of neuroendocrine tumours. (68)Ga-Dotatoc is prepared on-site through a so-called magisterial preparation. The use of an appropriate buffer during the radiolabelling step is essential to maximize the lab...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0b013e32833acb99

    authors: Bauwens M,Chekol R,Vanbilloen H,Bormans G,Verbruggen A

    更新日期:2010-08-01 00:00:00

  • Long-term follow-up using 18F-FDG PET/CT for postoperative olfactory neuroblastoma.

    abstract:PURPOSE:The present study evaluated the usefulness of postoperative fluorine-18 fluorodeoxyglucose (F-FDG) PET/computed tomography (CT) in the management of olfactory neuroblastoma (ONB). MATERIALS AND METHODS:Ten patients (eight men and two women; mean age, 48.5 years) with histologically confirmed ONB who underwent ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000000135

    authors: Fujioka T,Toriihara A,Kubota K,Machida Y,Nakamura S,Kishimoto S,Ohashi I,Shibuya H

    更新日期:2014-08-01 00:00:00

  • Lymphoscintigraphic details of sentinel lymph node detection in 82 patients with squamous cell carcinoma of the oral cavity and oropharynx.

    abstract::Lymphoscintigraphy for sentinel node (SN) detection has been studied extensively in melanoma and breast cancer. In head and neck squamous cell carcinoma (HNSCC), however, experience in this field is relatively meagre. The purpose of this study was to document and evaluate lymphoscintigraphic findings in HNSCC patients...

    journal_title:Nuclear medicine communications

    pub_type: 临床试验,杂志文章

    doi:10.1097/00006231-200306000-00006

    authors: Nieuwenhuis EJ,Pijpers R,Castelijns JA,Snow GB

    更新日期:2003-06-01 00:00:00

  • The role of fluorine-18 fluorodeoxyglucose PET in prognosis evaluation for stem cell transplantation of lymphoma: a systematic review and meta-analysis.

    abstract::The role of fluorine-18 fluorodeoxyglucose PET (F-FDG PET) in prognostic evaluation of pre-stem cell transplantation (SCT) and post-SCT is still uncertain. A systematic review and meta-analysis were carried out to detect the prognostic power of F-FDG PET. 'PubMed', EMBASE, and Springer were searched for relevant artic...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章,meta分析,评审

    doi:10.1097/MNM.0000000000000468

    authors: Wang C,Li P,Wu S,Lu J,Liu Q,Luo H,Song S

    更新日期:2016-04-01 00:00:00

  • Scintigraphic study of malignant melanoma with 123I-iodoamphetamine.

    abstract::N-isopropyl-p-I-123-iodoamphetamine (123I-IMP) is extracted by the brain in proportion to regional blood flow. It is particularly useful in the clinical assessment of various pathological conditions related to altered cerebral flow. Moreover, it has been reported that 123I-IMP is avidly incorporated into melanin and m...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199602000-00010

    authors: Watanabe N,Seto H,Yokoyama K,Matsuda H,Shimizu M,Tonami N,Hisada K,Kakishita M

    更新日期:1996-02-01 00:00:00

  • A 99Tcm-labelled leukotriene B4 receptor antagonist for scintigraphic detection of infection in rabbits.

    abstract::In a search for a rapid and accurate imaging agent for scintigraphic detection of infection and inflammation, an LTB4 receptor antagonist, 99Tcm-RP517, which contains the hydrazino nicotinamide moiety, has been developed recently. To study the in vivo behaviour of 99Tcm-RP517, rabbits with Escherichia coli infection w...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-200011000-00010

    authors: Brouwers AH,Laverman P,Boerman OC,Oyen WJ,Barrett JA,Harris TD,Edwards DS,Corstens FH

    更新日期:2000-11-01 00:00:00

  • Short-term and long-term monitoring of the serum level of TPA after radical resection of gastrointestinal or lung cancer.

    abstract::Radically operated tumour patients (gastrointestinal cancer, n = 10; lung cancer, n = 10) were subjected to continuous prospective monitoring of the serum level of tissue polypeptide antigen (TPA) prior to surgical treatment as well as during the subsequent year. The following observations were made: Immediately after...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-198602000-00007

    authors: Bauer T,Muhrer KH,Müller H,Grebe SF

    更新日期:1986-02-01 00:00:00

  • Molecular PET and PET/CT imaging of tumour cell proliferation using F-18 fluoro-L-thymidine: a comprehensive evaluation.

    abstract::Positron emission tomography (PET) using F-18 fluoro-3'-deoxy-3-L-fluorothymidine (FLT) offers noninvasive assessment of cell proliferation in vivo. The most important application refers to the evaluation of tumour proliferative activity, representing a key feature of malignancy. Most data to date suggest that FLT is ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章,评审

    doi:10.1097/MNM.0b013e32832ee93b

    authors: Barwick T,Bencherif B,Mountz JM,Avril N

    更新日期:2009-12-01 00:00:00

  • Reductions in finger doses for radiopharmaceutical dispensing afforded by a syringe shield and an automatic dose dispenser.

    abstract::A gamma extremity monitoring system (GEMS) has been used to measure finger doses during radio-nuclide dispensing procedures. GEMS uses a small semi-conductor probe that can be attached to a finger from which a continuous read-out can be obtained that is related to dose rate. The pattern of dose accumulation can be ana...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/00006231-199902000-00013

    authors: Montgomery A,Anstee DE,Martin CJ,Hilditch TE

    更新日期:1999-02-01 00:00:00

  • Meta-analysis of meta-analyses in oncologic positron emission tomography.

    abstract:OBJECTIVES:As PET has become a standard of treatment in patients with cancer, publications of oncologic PET have grown sharply. We aimed to organize the data of meta-analyses of oncologic PET and assess the quality of studies. MATERIALS AND METHODS:The inclusion criteria were studies that meta-analyzed to combine the ...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章,meta分析

    doi:10.1097/MNM.0000000000000908

    authors: Pak K,Shin S,Kim SJ,Kim K,Seo Y,Kim SJ,Kim IJ

    更新日期:2018-12-01 00:00:00

  • Comparison of treatment efficacy 1 and 2 years after thyroid remnant ablation with 1110 versus 5550 MBq of iodine-131 in patients with intermediate-risk differentiated thyroid cancer.

    abstract:BACKGROUND:Radioiodine ablation may be associated with improved survival in patients with intermediate-risk follicular cell differentiated thyroid cancer (FCDTC). The aim of this study was to compare ablation efficacy of 1110 versus 5500 MBq of iodine-131 (I) in FCDTC patients with intermediate risk. METHODS:Thirty-ni...

    journal_title:Nuclear medicine communications

    pub_type: 杂志文章

    doi:10.1097/MNM.0000000000000730

    authors: Aghaei A,Ayati N,Shafiei S,Abbasi B,Zakavi SR

    更新日期:2017-11-01 00:00:00