EGF cell surface receptor quantitation on ocular cells by an immunocytochemical flow cytometry technique.

abstract：PURPOSE:To investigate whether polymorphisms in the gene encoding the chemokine receptor CX3CR1, which has been linked to changes in functional ligand-binding activity, are associated with retinal vasculitis (RV) in a cohort of patients in the United Kingdom. METHODS:DNA was prepared from whole blood of 126 patients w...

journal_title：Investigative ophthalmology & visual science

authors： Wallace GR,Vaughan RW,Kondeatis E,Mathew R,Chen Y,Graham EM,Stanford MR

更新日期：2006-07-01 00:00:00

abstract：:An imaging fundus reflectometer has been used to study the distribution and regeneration of visual pigments in the retina of the adult living cat. Measurements were made over an area of tapetal retina extending 25 degrees nasal and 40 degrees temporal to the area centralis on the horizontal meridian and 5 degrees infe...

journal_title：Investigative ophthalmology & visual science

authors： Kemp CM,Faulkner DJ,Jacobson SG

更新日期：1988-07-01 00:00:00

abstract：:The mouse retina has been used extensively over the past decades to study both physiologic and pathologic angiogenesis. Over time, various mouse retina models have evolved into well-characterized and robust tools for in vivo angiogenesis research. This article is a review of the angiogenic development of the mouse ret...

journal_title：Investigative ophthalmology & visual science

authors： Stahl A,Connor KM,Sapieha P,Chen J,Dennison RJ,Krah NM,Seaward MR,Willett KL,Aderman CM,Guerin KI,Hua J,Löfqvist C,Hellström A,Smith LE

更新日期：2010-06-01 00:00:00

abstract：:The irides of albino guinea pigs were photographed, and the iris arteriolar diameters were measured with the animal breathing room air, 10% O2/90% N2, or 100% O2. The iris arterioles constrict significantly in hyperoxia and dilate in hypoxia. The average constriction in 100% O2 was 27% +/- 8%, and the average dilatati...

journal_title：Investigative ophthalmology & visual science

authors： Stefansson E,Shelton RA,Landers MB 3rd,Walsh AW,Wolbarsht ML

更新日期：1983-06-01 00:00:00

abstract：PURPOSE:To evaluate the importance of lens epithelial cell death in age-related cataract. To determine whether the large percentage of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive lens epithelial cells previously reported in human capsulotomy specimens results from apopto...

journal_title：Investigative ophthalmology & visual science

authors： Harocopos GJ,Alvares KM,Kolker AE,Beebe DC

更新日期：1998-12-01 00:00:00

abstract：Purpose:Neuroretinopathy is increasingly being recognized as an independent cause of vision loss in diabetes. Visual field loss, as detected by frequency doubling technology (FDT)-based visual perimetry, is a sign of neuroretinopathy and occurs in early stages of diabetic retinopathy (DR). Here, we hypothesized that FD...

journal_title：Investigative ophthalmology & visual science

authors： Bao YK,Yan Y,Gordon M,McGill JB,Kass M,Rajagopal R

更新日期：2019-11-01 00:00:00

abstract：PURPOSE:The purpose of this study was to determine the relationship between optical coherence tomography (OCT) measures of retinal nerve fiber layer (RNFL) and neuroretinal rim (NRR) in a nonhuman primate experimental glaucoma model, and in a population of clinical patients. METHODS:For nonhuman primates, normative da...

journal_title：Investigative ophthalmology & visual science

authors： Patel NB,Sullivan-Mee M,Harwerth RS

更新日期：2014-09-23 00:00:00

abstract：:Freshly isolated peripheral blood lymphocytes from patients (PBL-P) with recurrent herpetic corneal and skin lesions, and controls (PBL-C) with no recollection of herpetic disease, effectively lysed herpes simplex virus type 1 (HSV-1) infected but not uninfected allogeneic fibroblasts. However, after 48 hr of stimulat...

journal_title：Investigative ophthalmology & visual science

authors： Hendricks RL,Sugar J

更新日期：1985-02-01 00:00:00

abstract：PURPOSE:Changes in gene expression were investigated after treatment of cultured human retinal pigment epithelial (RPE) cells with vitreous. This may have implications for proliferative diseases such as proliferative vitreoretinopathy. METHODS:Cells were cultured in the presence or absence of human vitreous, and gene ...

journal_title：Investigative ophthalmology & visual science

authors： Hunt DM,Chen WH,Hunt RC

更新日期：1998-10-01 00:00:00

abstract：PURPOSE:To evaluate immunohistochemical staining of the endothelia of corneas from patients with clinical diagnoses of iridocorneal endothelial (ICE) syndrome. METHODS:Corneas diagnosed with ICE syndrome and removed during corneal transplantation were freshly frozen, sectioned, and stained with monoclonal antibodies t...

journal_title：Investigative ophthalmology & visual science

authors： Hirst LW,Bancroft J,Yamauchi K,Green WR

更新日期：1995-04-01 00:00:00

abstract：PURPOSE:To investigate macular changes in acute retinopathy of prematurity (ROP). METHODS:Fifty-four premature infants with ROP and 20 controls underwent routine ROP screening with indirect ophthalmoscopy and imaging. A tabletop spectral domain optical coherence tomography (SD-OCT) scanner (Spectralis; Heidelberg Engi...

journal_title：Investigative ophthalmology & visual science

authors： Vinekar A,Avadhani K,Sivakumar M,Mahendradas P,Kurian M,Braganza S,Shetty R,Shetty BK

更新日期：2011-07-15 00:00:00

abstract：:Nonenzymatic glycated lens alpha-crystallin, isolated from human diabetic cataract lenses, does not differ in subunit size and secondary structure from nonglycated alpha-crystallin. The tertiary structure, however, has undergone a significant change as reflected in the changes of near-ultraviolet (UV) circular dichroi...

journal_title：Investigative ophthalmology & visual science

authors： Liang JN,Chylack LT Jr

更新日期：1987-05-01 00:00:00

abstract：Purpose:To compare the visual field (VF) progression among high tension glaucoma (HTG), primary angle closure glaucoma (PACG), and normal tension glaucoma (NTG) subjects in routine clinical care. Methods:All patients had ≥5 VF tests using HFA, 24-2, SITA-standard strategy. We compared the progression between glaucoma ...

journal_title：Investigative ophthalmology & visual science

authors： Ballae Ganeshrao S,Senthil S,Choudhari N,Sri Durgam S,Garudadri CS

更新日期：2019-03-01 00:00:00

abstract：Purpose:Most of the inflammation in murine herpes simplex virus type 1 (HSV-1)-induced stromal keratitis (HSK) is due to exposure stress resulting from loss of corneal nerves and blink reflex. Corneal grafts often fail when placed on corneal beds with a history of HSK. We asked if corneal exposure contributes to the se...

journal_title：Investigative ophthalmology & visual science

authors： Rowe AM,Yun H,Hendricks RL

更新日期：2017-01-01 00:00:00

abstract：PURPOSE:To identify risk factors for the development of high-risk proliferative diabetic retinopathy (PDR) and for the development of severe visual loss or vitrectomy (SVLV) in eyes assigned to deferral of photocoagulation in the Early Treatment Diabetic Retinopathy Study (ETDRS). METHODS:Multivariable Cox models were...

journal_title：Investigative ophthalmology & visual science

authors： Davis MD,Fisher MR,Gangnon RE,Barton F,Aiello LM,Chew EY,Ferris FL 3rd,Knatterud GL

更新日期：1998-02-01 00:00:00

abstract：PURPOSE:To visualize changes in deep optic nerve head (ONH) structures following glaucoma surgery using (3-dimensional [3D]) swept-source optical coherence tomography (SS-OCT) and to determine the clinical and structural factors associated with postoperative lamina cribrosa (LC) and prelaminar neural tissue (PLT) chang...

journal_title：Investigative ophthalmology & visual science

authors： Yoshikawa M,Akagi T,Hangai M,Ohashi-Ikeda H,Takayama K,Morooka S,Kimura Y,Nakano N,Yoshimura N

更新日期：2014-01-23 00:00:00

abstract：Purpose:Mutations in CACNA2D4, encoding the α2δ4 subunit of retinal voltage-gated calcium channels (Cav), cause a rare type of retinal dysfunction in human, mainly affecting cone vision. Here, we investigate the role of CACNA2D4 in targeting of Cav, its influence on cone-mediated signal transmission, and the cellular a...

journal_title：Investigative ophthalmology & visual science

authors： Schlegel DK,Glasauer SMK,Mateos JM,Barmettler G,Ziegler U,Neuhauss SCF

更新日期：2019-12-02 00:00:00

abstract：PURPOSE:To compare anterior segment parameters, assessed by anterior segment optical coherence tomography (ASOCT), in subjects categorized as primary angle closure suspect (PACS), primary angle closure (PAC), primary angle closure glaucoma (PACG), and previous acute PAC (APAC); and to identify factors associated with A...

journal_title：Investigative ophthalmology & visual science

authors： Guzman CP,Gong T,Nongpiur ME,Perera SA,How AC,Lee HK,Cheng L,He M,Baskaran M,Aung T

更新日期：2013-08-07 00:00:00

abstract：PURPOSE:Elevated levels of extracellular glutamate have been implicated in the pathophysiology of neuronal loss in both central nervous system and ophthalmic disorders, including glaucoma. This increase in glutamate may result from a failure of glutamate transporters (molecules that ordinarily regulate extracellular gl...

journal_title：Investigative ophthalmology & visual science

authors： Vorwerk CK,Naskar R,Schuettauf F,Quinto K,Zurakowski D,Gochenauer G,Robinson MB,Mackler SA,Dreyer EB

更新日期：2000-10-01 00:00:00

abstract：PURPOSE:To examine immune processes in the lacrimal gland of patients with chronic graft-versus-host disease (cGVHD) by evaluating the expression of surface molecules associated with T-cell activation. METHODS:Antibodies to CD4, CD8, CD34, CD40, CD54, CD80, CD86, CD154, and HLA-DR were used for immunohistochemical ana...

journal_title：Investigative ophthalmology & visual science

authors： Ogawa Y,Kuwana M,Yamazaki K,Mashima Y,Yamada M,Mori T,Okamoto S,Oguchi Y,Kawakami Y

更新日期：2003-05-01 00:00:00

abstract：PURPOSE:High intraocular pressure (IOP) is a major risk factor for glaucoma, one of the leading causes of blindness worldwide. Because it has been demonstrated that African populations are at increased risk for glaucoma, the authors investigated the genetic basis of IOP in a sample of West Africans with type 2 diabetes...

journal_title：Investigative ophthalmology & visual science

authors： Rotimi CN,Chen G,Adeyemo AA,Jones LS,Agyenim-Boateng K,Eghan BA Jr,Zhou J,Doumatey A,Lashley K,Huang H,Fasanmade O,Akinsola FB,Ezepue F,Amoah A,Akafo S,Chen Y,Oli J,Johnson T

更新日期：2006-08-01 00:00:00

abstract：PURPOSE:To investigate the function of connective tissue growth factor (CTGF), a matricellular protein of the CCN (Cyr61/CTGF/Nov) family, in retinal vasculature during development and ischemia. METHODS:CTGF expression was determined using RT-PCR, immunohistochemistry, and transgenic mice carrying CTGF promoter-driven...

journal_title：Investigative ophthalmology & visual science

authors： Pi L,Xia H,Liu J,Shenoy AK,Hauswirth WW,Scott EW

更新日期：2011-11-07 00:00:00

abstract：PURPOSE:The Rd4/+ mouse inherits an autosomal dominant retinal degeneration that cosegregates with a large inversion spanning nearly all of mouse chromosome 4 (Chr 4). This inversion is homozygous lethal. The hypothesis for the study was that disruption of a gene at one of the two breakpoints in the Rd4 chromosome is r...

journal_title：Investigative ophthalmology & visual science

authors： Kitamura E,Danciger M,Yamashita C,Rao NP,Nusinowitz S,Chang B,Farber DB

更新日期：2006-04-01 00:00:00

abstract：PURPOSE:The goals of this study were to evaluate the safety of office-based vitreous sampling, and determine the utility of these samples with multiplex cytokine analysis. METHODS:Vitreous samples were collected from office-based needle aspiration and the rate of adverse events during follow-up was reviewed. The vitre...

journal_title：Investigative ophthalmology & visual science

authors： Ghodasra DH,Fante R,Gardner TW,Langue M,Niziol LM,Besirli C,Cohen SR,Dedania VS,Demirci H,Jain N,Jayasundera KT,Johnson MW,Kalyani PS,Rao RC,Zacks DN,Sundstrom JM

更新日期：2016-06-01 00:00:00

abstract：PURPOSE:To investigate macular photoreceptor structure in patients with inherited retinal degeneration using high-resolution images and to correlate the findings with clinical phenotypes and genetic mutations. METHODS:Adaptive optics scanning laser ophthalmoscopy (AOSLO) images of photoreceptors were obtained in 16 ey...

journal_title：Investigative ophthalmology & visual science

authors： Duncan JL,Zhang Y,Gandhi J,Nakanishi C,Othman M,Branham KE,Swaroop A,Roorda A

更新日期：2007-07-01 00:00:00

abstract：PURPOSE:To design a glucocorticoid-inducible virus vector overexpressing recombinant matrix metalloproteinase 1 (MMP1) and counteract extracellular matrix deposition in the trabecular meshwork only when steroid is present. METHODS:Endogenous MMP1 expression was measured in primary human trabecular meshwork cells (HTM)...

journal_title：Investigative ophthalmology & visual science

authors： Spiga MG,Borrás T

更新日期：2010-06-01 00:00:00

abstract：:A simple method for the culture of human retinal capillary endothelial cells in vitro is described in this report. Through a process of gentle mechanical disruption and sieving, a sufficient yield of retinal microvessels was obtained from one or two human eyes to allow the culture of endothelial cells in abundance. Th...

journal_title：Investigative ophthalmology & visual science

authors： Su T,Gillies MC

更新日期：1992-09-01 00:00:00

abstract：PURPOSE:We investigated the mechanism of tamoxifen (TAM) retinotoxicity using human retinal pigment epithelial (RPE)-derived (ARPE-19) and photoreceptor-derived (661W) cells. METHODS:Cultured ARPE-19 and 661W cells were treated with 5 to 10 μM TAM, and the resultant cell death was quantified using lactate dehydrogenas...

journal_title：Investigative ophthalmology & visual science

authors： Cho KS,Yoon YH,Choi JA,Lee SJ,Koh JY

更新日期：2012-08-09 00:00:00

abstract：PURPOSE:Slow oscillatory eye movements (SOMs) occur simultaneously with tremor, drifts, and microsaccades during visual fixation. In a previous study, the amplitude of SOM was found to be affected by the visual characteristics of the stimuli. This indicates a perceptual influence on the control of the movement. However...

journal_title：Investigative ophthalmology & visual science

authors： Zhang B,Bolzani R,Öqvist Seimyr G,Ygge J,Pansell T

更新日期：2013-12-11 00:00:00

abstract：PURPOSE:MRL/Mp-lpr/lpr mice (MRL/lpr) spontaneously develop lacrimal gland inflammatory lesions and are a model for the human disease Sjögren's syndrome. Therapy with monoclonal antibodies (mAb) to CD4 ameliorates the autoimmune renal, vasculitic, and intraocular inflammatory lesions in MRL/lpr mice. The effect of anti...

journal_title：Investigative ophthalmology & visual science

authors： Jabs DA,Burns WH,Prendergast RA

更新日期：1996-01-01 00:00:00