The power of chemical genomics to study the link between endomembrane system components and the gravitropic response.

abstract：:Intracellular bacterial pathogens evade the bactericidal functions of mammalian cells by physical escape from their phagosome and replication into the cytoplasm or through the modulation of phagosome maturation and biogenesis of a membrane-bound replicative organelle. Here, we detail in murine primary macrophages the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Checroun C,Wehrly TD,Fischer ER,Hayes SF,Celli J

更新日期：2006-09-26 00:00:00

abstract：:RS67333 is a partial serotonin subtype 4 receptor (5-HT4R) agonist that has been widely studied for its procognitive effect. More recently, it has been shown that its ability to promote the nonamyloidogenic cleavage of the precursor of the neurotoxic amyloid-β peptide leads to the secretion of the neurotrophic protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lecoutey C,Hedou D,Freret T,Giannoni P,Gaven F,Since M,Bouet V,Ballandonne C,Corvaisier S,Malzert Fréon A,Mignani S,Cresteil T,Boulouard M,Claeysen S,Rochais C,Dallemagne P

更新日期：2014-09-09 00:00:00

abstract：:The sloppy paired (slp) locus consists of two genes, slp1 and slp2, both of which encode proteins containing a forkhead domain (a DNA-binding motif). Previous work has shown that a severe segmentation phenotype is obtained only when both slp genes are deleted. Here we examine the functional redundancy of the locus in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cadigan KM,Grossniklaus U,Gehring WJ

更新日期：1994-07-05 00:00:00

abstract：:Residues important in the interaction between the 23-residue transmembrane (TM) domains of the integrin alpha(IIb)- and beta(3)-subunits were identified by mutating each non-Leu residue to Leu. Leu substitutions of alpha(IIb) at G972, G976, and T981, and of beta(3) at I693 and G708, increased ligand binding. Substitut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo BH,Carman CV,Takagi J,Springer TA

更新日期：2005-03-08 00:00:00

abstract：:The inability to perceive and coordinate both internal and external signals that function to regulate cellular growth and proliferation is a hallmark of oncogenic transformation. To examine the effects of the v-mos oncogene on distinct signal transduction pathways, the 6m2 cell line was used, in which expression of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamilton BJ,DeFranco D

更新日期：1989-01-01 00:00:00

abstract：:Femtosecond and subfemtosecond time scales typically rule electron dynamics at metal surfaces. Recent advance in experimental techniques permits now remarkable precision in the description of these processes. In particular, shorter time scales, smaller system sizes, and spin-dependent effects are current targets of in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muiño RD,Sánchez-Portal D,Silkin VM,Chulkov EV,Echenique PM

更新日期：2011-01-18 00:00:00

abstract：:Drosophila melanogaster is a powerful model organism for dissecting the neurogenetic basis of appetitive and aversive behaviors. However, some methods used to assay food preference require or cause starvation. This can be problematic for fly ethanol research because it can be difficult to dissociate caloric preference...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park A,Tran T,Atkinson NS

更新日期：2018-09-04 00:00:00

abstract：:A half-century's worth of research has established the existence of numerous event-related potential components measuring different cognitive operations in humans including the selection of stimuli by covert attention mechanisms. Surprisingly, it is unknown whether nonhuman primates exhibit homologous electrophysiolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woodman GF,Kang MS,Rossi AF,Schall JD

更新日期：2007-09-18 00:00:00

abstract：:Oxidation of genomic DNA forms the guanine lesion 7,8-dihydro-8-oxoguanine (8-oxoG). When in the template base position during DNA synthesis the 8-oxoG lesion has dual coding potential by virtue of its anti- and syn-conformations, base pairing with cytosine and adenine, respectively. This impacts mutagenesis, because ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Batra VK,Shock DD,Beard WA,McKenna CE,Wilson SH

更新日期：2012-01-03 00:00:00

abstract：:Colloidal particles with directional interactions are key in the realization of new colloidal materials with possibly unconventional phase behaviors. Here we exploit DNA self-assembly to produce bulk quantities of "DNA stars" with three or four sticky terminals, mimicking molecules with controlled limited valence. Sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biffi S,Cerbino R,Bomboi F,Paraboschi EM,Asselta R,Sciortino F,Bellini T

更新日期：2013-09-24 00:00:00

abstract：:We demonstrate that the BC200 RNA gene, which encodes a neural small cytoplasmic RNA, is a member of the most prodigious family of interspersed repetitive DNA and that its product represents an example of a primate tissue-specific RNA polymerase III transcript. The BC200 RNA gene is an early monomeric member and one o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martignetti JA,Brosius J

更新日期：1993-12-15 00:00:00

abstract：:The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to acce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ryan KS,Howard-Jones AR,Hamill MJ,Elliott SJ,Walsh CT,Drennan CL

更新日期：2007-09-25 00:00:00

abstract：:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin that causes parkinsonism in humans and nonhuman animals, and its use has led to greater understanding of the pathogenesis of Parkinson's disease. However, its molecular targets have not been defined. We show that mice lacking the gene for poly(ADP-rib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandir AS,Przedborski S,Jackson-Lewis V,Wang ZQ,Simbulan-Rosenthal CM,Smulson ME,Hoffman BE,Guastella DB,Dawson VL,Dawson TM

更新日期：1999-05-11 00:00:00

abstract：:Excitatory amino acid transporters (EAATs) buffer and remove synaptically released L-glutamate and maintain its concentrations below neurotoxic levels. EAATs also mediate a thermodynamically uncoupled substrate-gated anion conductance that may modulate cell excitability. Here, we demonstrate that modification of a cys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seal RP,Shigeri Y,Eliasof S,Leighton BH,Amara SG

更新日期：2001-12-18 00:00:00

abstract：:Interferon regulatory factor (IRF) 4 is a member of the IRF family of transcription factors and plays critical roles in the development of CD4(+) T cells into Th2 and Th17 cells. Using the infection model of Nippostrongyrus brasiliensis, we have confirmed the critical roles of IRF-4 in Th2 development in vivo by using...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honma K,Kimura D,Tominaga N,Miyakoda M,Matsuyama T,Yui K

更新日期：2008-10-14 00:00:00

abstract：:After exposure of segments of rabbit taenia coli to the nonionic detergent Triton X-100, tension could be induced by increasing the [Ca2+] in the micromolar range. In the presence of a saturating [Ca2+], this preparation developed nearly 100% of the control tetanus tension recorded from the intact muscle prior to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon AR

更新日期：1978-07-01 00:00:00

abstract：:Cell adhesion molecules (CAMs) are cell surface glycoproteins that may play a variety of roles in morphogenesis and histogenesis, particularly in defining borders of discrete cell populations. To examine the influence of CAM expression on such cell segregation events in vitro, we have transfected cells with cDNAs codi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedlander DR,Mège RM,Cunningham BA,Edelman GM

更新日期：1989-09-01 00:00:00

abstract：:Results on the subcellular localization of the mineralocorticoid receptor (MR) have been controversial. To determine the subcellular distribution and trafficking of the MR in living cells after binding of agonists and antagonists, we expressed a MR-green fluorescent protein (GFP) chimera in mammalian cells lacking end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fejes-Tóth G,Pearce D,Náray-Fejes-Tóth A

更新日期：1998-03-17 00:00:00

abstract：:Expression cloning of cDNAs was first described a decade ago and was based on transient expression of cDNA libraries in COS cells. In contrast to transient transfection of plasmids, retroviral gene transfer delivers genes stably into a wide range of target cells. We utilize a simple packaging system for production of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kitamura T,Onishi M,Kinoshita S,Shibuya A,Miyajima A,Nolan GP

更新日期：1995-09-26 00:00:00

abstract：:Little is known about the catalytic mechanism of the recently discovered ThyX family of flavin-dependent thymidylate synthases that are required for thymidylate (deoxythymidine 5'-monophosphate) synthesis in a large number of microbial species. Using a combination of site-directed mutagenesis and biochemical measureme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leduc D,Graziani S,Lipowski G,Marchand C,Le Maréchal P,Liebl U,Myllykallio H

更新日期：2004-05-11 00:00:00

abstract：:Prey shifts in carnivorous predators are events that can initiate the accelerated generation of new biodiversity. However, it is seldom possible to reconstruct how the change in prey preference occurred. Here we describe an evolutionary "smoking gun" that illuminates the transition from worm hunting to fish hunting am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aman JW,Imperial JS,Ueberheide B,Zhang MM,Aguilar M,Taylor D,Watkins M,Yoshikami D,Showers-Corneli P,Safavi-Hemami H,Biggs J,Teichert RW,Olivera BM

更新日期：2015-04-21 00:00:00

abstract：:We extend and apply theories of filled foam elasticity and failure to recently available data on foods. The predictions of elastic modulus and failure mode dependence on internal pressure and on wall integrity are borne out by photographic evidence of distortion and failure under compressive loading and under the loca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warner M,Thiel BL,Donald AM

更新日期：2000-02-15 00:00:00

abstract：:Accounting for enzyme-mediated active sliding, disassembly, and sequence-dependent positioning of nucleosomes, we simulate nucleosome occupancy over cell-cycle-scale times using a stochastic kinetic model. We show that ATP-dependent active nucleosome sliding and nucleosome removal processes are essential to obtain in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Padinhateeri R,Marko JF

更新日期：2011-05-10 00:00:00

abstract：:2-[4-(3,4-Dichlorophenylureido)phenoxy]-2-methylpropionic acid, LR16, combines with two symmetrically related sites in the central cavity of deoxyhemoglobin, 20 A away from the binding site of 2,3-bisphosphoglycerate, and acts as an allosteric effector synergistic with 2,3-bisphosphoglycerate. LR16 (1 mM) raises P50, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lalezari I,Rahbar S,Lalezari P,Fermi G,Perutz MF

更新日期：1988-08-01 00:00:00

abstract：:Previous work on perceptual decision making in the sensorimotor system has shown population dynamics in the beta band, corresponding to the encoding of stimulus properties and the final decision outcome. Here, we asked how oscillatory dynamics in the medial premotor cortex (MPC) contribute to supramodal perceptual dec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haegens S,Vergara J,Rossi-Pool R,Lemus L,Romo R

更新日期：2017-12-26 00:00:00

abstract：:Membrane lipids were once thought to be homogenously distributed in the 2D surface of a membrane, but the lipid raft theory suggests that cholesterol and sphingolipids partition away from other membrane lipids. Lipid raft theory further implicates these cholesterol-rich domains in many processes such as signaling and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foster LJ,De Hoog CL,Mann M

更新日期：2003-05-13 00:00:00

abstract：:Some realistic galaxy models have been found to support discrete unstable spiral modes. Here, through the study of the relevant physical mechanisms and an extensive numerical investigation of the properties of the dominant modes in a wide class of galactic equilibria, we show how spiral structures are excited with dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haass J,Bertin G,Lin CC

更新日期：1982-06-01 00:00:00

abstract：:Understanding the mechanisms by which compounds discovered using cell-based phenotypic screening strategies might exert their effects would be highly augmented by new approaches exploring their potential interactions with the genome. For example, altered androgen receptor (AR) transcriptional programs, including castr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin C,Yang L,Xie M,Lin C,Merkurjev D,Yang JC,Tanasa B,Oh S,Zhang J,Ohgi KA,Zhou H,Li W,Evans CP,Ding S,Rosenfeld MG

更新日期：2014-06-24 00:00:00

abstract：:Splenic cells from one BALB/c mouse and one C57/BL mouse, immunized with purified rat liver glucocorticoid receptor (GR), were fused with the mouse myeloma cell line Sp 2/0-Ag 14. Screening for production of anti-GR-antibodies by the hybridomas was carried out with an enzyme-linked immunosorbent assay, using partially...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okret S,Wikström AC,Wrange O,Andersson B,Gustafsson JA

更新日期：1984-03-01 00:00:00

abstract：:Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez JA,Pierce KE,Rice JE,Wangh LJ

更新日期：2004-02-17 00:00:00