Abstract:
BACKGROUND & AIMS:Monocytes/macrophages infiltrate into injured livers. We tried to clarify their roles in inflammation and subsequent fibrogenesis by inhibiting their infiltration with a mutated form (7ND; 7 amino acids at the N-terminal were deleted) of monocyte chemoattractant protein 1, which may function as a dominant-negative mutant. METHODS:Rats were injected via the tail vein with an adenovirus expressing either human 7ND (Ad7ND), a truncated type II transforming growth factor beta receptor (AdTbeta-TR), which works as a dominant-negative receptor, bacterial beta-galactosidase (AdLacZ), or saline. Seven days later, the rats were treated with dimethylnitrosamine for 1-21 days. RESULTS:Within 24 hours after a single dimethylnitrosamine injection, macrophages were observed in livers. With a 3-day dimethylnitrosamine treatment, activated hepatic stellate cells were detectable in livers in AdLacZ-, AdTbeta-TR-, and saline-injected rats. In contrast, in the Ad7ND-treated rats, infiltration of macrophages was markedly reduced, and activated hepatic stellate cells were not detectable. After a 3-week dimethylnitrosamine treatment, fibrogenesis was almost completely inhibited, and activated hepatic stellate cells were hardly seen in livers in both Ad7ND- and AdTbeta-TR-treated rats. CONCLUSIONS:Our results show that blockade of macrophage infiltration inhibits activation of hepatic stellate cells and leads to suppression of liver fibrogenesis. The presence of activated hepatic stellate cells in the initial phase after injury and its absence at a later phase in the AdTbeta-TR-treated livers indicate that transforming growth factor beta is not an activating factor for hepatic stellate cells, and this suggests that transforming growth factor beta is required for the survival of activated hepatic stellate cells. Our study suggests that infiltrated macrophages may themselves produce an activating factor for hepatic stellate cells.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Imamura M,Ogawa T,Sasaguri Y,Chayama K,Ueno Hdoi
10.1053/j.gastro.2004.10.005keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
138-46issue
1eissn
0016-5085issn
1528-0012pii
S0016508504019225journal_volume
128pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:In the Active Ulcerative Colitis Trial (ACT)-1 and ACT-2, patients with ulcerative colitis treated with infliximab were more likely than those given placebo to have a clinical response, undergo remission, and have mucosal healing. We investigated the association between early improvement (based on end...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2011.06.054
更新日期:2011-10-01 00:00:00
abstract::Individual l-amino acids were instilled intragastrically to determine possible differences in stimulation of gastric acid secretion and gastrin and pancreatic polypeptide release. Phenylalanine and tryptophan were significantly more potent stimulants of gastric acid secretion and of pancreatic polypeptide and gastrin ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract:BACKGROUND & AIMS:The ratio of liver size to body weight (hepatostat) is tightly controlled, but little is known about how the physiologic functions of the liver help determine its size. Livers of mice repopulated with human hepatocytes (humanized livers) grow to larger than normal; the human hepatocytes do not recogni...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.05.043
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND & AIMS:Tobacco smoking has a complex effect on intestinal inflammation, being protective in ulcerative colitis, whereas it aggravates Crohn's disease. The beneficial effect of smoking has been attributed to nicotine, but the mechanisms underlying the adverse effect are still under investigation. The aim of t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70346-x
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND & AIMS:Math1 (Atoh1) is a basic helix-loop-helix transcription factor important for intestinal secretory cell differentiation. We hypothesized that Math1 is important in cell fate commitment, and therefore mediates proliferative homeostasis and the adaptive response following intestinal resection in the adul...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.03.047
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND & AIMS:Despite high morbidity and mortality of alcoholic liver disease worldwide, the molecular mechanisms underlying alcohol-induced liver cell death are not fully understood. Transglutaminase 2 (TG2) is a cross-linking enzyme implicated in apoptosis. TG2 levels and activity are increased in association wit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.01.007
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND & AIMS:Cyclosporin A specifically suppresses hepatitis C virus (HCV) replication in vitro at clinically achievable concentrations. In this study, we investigated the mechanisms of action of cyclosporin A against HCV replication. METHODS:The in vitro effects of cyclosporin A on HCV replication were analyzed ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.06.031
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND & AIMS:Calcitonin gene-related peptide (CGRP) protects the gastric mucosa against injurious stimuli in various experimental models. The underlying mechanism could be the increase in gastric mucosal blood flow (GMBF). A number of endogenous vasodilators exert their effects through the activation of adenosine ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70634-1
更新日期:1998-01-01 00:00:00
abstract::Ileocecal sphincter pressure was studied in the cat, using an open-tipped perfused catheter system. Ileocecal sphincter pressure was measured under basal conditions and in response to bethanechol or direct vagal nerve electrical stimulation. The pressure was 26.1 +/- 5.0 mmHg basally, and was unaltered by section of e...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND & AIMS:The emergence of oxyntic atrophy and metaplastic cell lineages in response to chronic Helicobacter pylori infection predisposes to gastric neoplasia. We have described a trefoil factor family 2 (TFF2; spasmolytic polypeptide) expressing metaplasia (SPEM) associated with gastric neoplasia in both roden...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2004.05.029
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND & AIMS:The mechanisms by which mutations in the familial intrahepatic cholestasis-1 gene cause Byler's disease (progressive familial intrahepatic cholestasis type 1) are unknown. METHODS:Interactions among the apical sodium-dependent bile acid transporter, the farnesoid X receptor (FXR), and familial intrah...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2003.12.013
更新日期:2004-03-01 00:00:00
abstract::The mortality experienced by a cohort of 653 patients with celiac disease in Edinburgh and the Lothian region has been analyzed. Mortality overall was 1.9-fold (95% confidence limits, 1.5-2.2) that of the general population (115 deaths observed, 61.8 expected; p less than 0.0001). The increased mortality was greatest ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90060-7
更新日期:1989-08-01 00:00:00
abstract:BACKGROUND/AIMS:Human colonic epithelium produces large amounts of mucin. The aim of this study was to examine mucin biosynthesis in the human colon. METHODS:Human colonic mucin was isolated using CsCl density gradients, and polyclonal antiserum was raised. Biosynthesis of colonic mucins was studied by labeling coloni...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90537-1
更新日期:1994-11-01 00:00:00
abstract::Gastroenterologists frequently encounter pelvic floor disorders, which affect 10% to 15% of the population. The anorectum is a complex organ that collaborates with the pelvic floor muscles to preserve fecal continence and enable defecation. A careful clinical assessment is critical for the diagnosis and management of ...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2013.10.062
更新日期:2014-01-01 00:00:00
abstract::The effects of cisapride on jejunal interdigestive motor activity were studied in 12 healthy men participating in three experiments each. Five minutes after an activity front (phase III) they received, in random double-blind fashion, 10 mg of cisapride, 4 mg of cisapride, or saline placebo by intravenous injection. Mo...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(86)90387-2
更新日期:1986-05-01 00:00:00
abstract:BACKGROUND & AIMS:Synchronous activation of the Wnt signaling pathway, mostly because of loss of function of the APC tumor suppressor, and of the oncogenic KRAS-signaling pathway is very frequent in colorectal cancer and is associated with poor prognosis. METHODS:We have generated a compound transgenic mouse model, KR...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.08.011
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND & AIMS:Paneth cells (PCs) secrete defensins and antimicrobial enzymes that contribute to innate immunity against pathogen infections within the mucosa of the small intestine. We examined the role of colony stimulating factor-1 (CSF-1) in PC development. METHODS:CSF-1-deficient and CSF-1 receptor (CSF-1R)-de...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.03.004
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND & AIMS:Treatment of hepatitis C virus (HCV) infection with boceprevir, peginterferon, and ribavirin can lead to anemia, which has been managed by reducing ribavirin dose and/or erythropoietin therapy. We assessed the effects of these anemia management strategies on rates of sustained virologic response (SVR)...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2013.07.051
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND & AIMS:Conflicting results have been published concerning the effects of different opioid-receptor agonists against visceral noxious stimulation. The introduction of colorectal distention facilitates research in this field. The aim of this study was to examine intrathecally administered opioid agonists again...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v110.pm8536850
更新日期:1996-01-01 00:00:00
abstract::The published world literature on the efficacy of cimetidine, a histamine H2-receptor antagonist, in the treatment of duodenal ulcer is reviewed. In eight prospective randomized double blind placebo-controlled studies, cimetidine was administered to 348 duodenal ulcer patients with an incidence of endoscopically verif...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1978-02-01 00:00:00
abstract:BACKGROUND & AIMS:Little is known about the prevalence of nonalcoholic fatty liver disease (NAFLD) among severely obese adolescents or factors that determine its development. We investigated the prevalence of NAFLD in a multicenter cohort of adolescents undergoing bariatric surgery and the factors associated with it. ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2015.05.039
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND & AIMS:Tumors that develop in patients with Crohn's disease tend be multifocal, so field cancerization (the replacement of normal cells with nondysplastic but tumorigenic clones) might contribute to intestinal carcinogenesis. We investigated patterns of tumor development from pretumor intestinal cell clones....
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.12.004
更新日期:2012-04-01 00:00:00
abstract::We studied the effect of chronic aspirin ingestion on gastroduodenal epithelial proliferation by feeding rats aspirin in the drinking water. A control group of rats received plain water. At the end of 4 wk, [3H]-thymidine was given intravenously to label proliferating cells, and the rats were killed 1 h later. Section...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::Two patients with hormone-secreting metastatic tumors of the liver (carcinoid, islet cell) were treated with transcatheter arterial embolization. In both cases, occlusion of blood supply to the tumors was accomplished, resulting in cessation of the systemic symptoms. Arterial embolization provides another useful palli...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract:BACKGROUND/AIMS:Conventional flow-cytometric analysis of colorectal tumor DNA content includes both tumor and stromal cell DNA. To improve the method, we evaluated the DNA content of purified epithelial cells using the crypt-isolation technique. METHODS:Normal and neoplastic crypts were isolated from fresh resected co...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(94)94651-5
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND & AIMS:Recently, the immediate early gene h-sgk was cloned as a hypertonicity-induced gene from human hepatoma cells. The aim of this study was to localize h-sgk messenger RNA (mRNA) expression in normal and inflamed intestinal mucosa and to identify potential transcriptional regulators. METHODS:h-sgk mRNA ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70011-9
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND & AIMS:Cholecystokinin (CCK) is a satiation peptide released during meals in response to lipid intake; it regulates pancreatic digestive enzymes that are required for absorption of nutrients. We proposed that mice with a disruption in the CCK gene (CCK knockout [CCK-KO] mice) that were fed a diet of 20% butt...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.01.044
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND & AIMS:The risk for colorectal cancer (CRC) in patients with inflammatory bowel disease (IBD) could have changed over time, with changes in treatment options. We studied CRC risk in a nationwide cohort of 47,374 Danish patients with IBD over a 30-year period. METHODS:We determined relative risk (RR) values ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.04.016
更新日期:2012-08-01 00:00:00
abstract::A patient with a lifelong history of asthma and hay fever was investigated because of symptoms of esophageal spasm. Esophageal biopsies revealed elongated papillae and basal zone hyperplasia of the epithelial layer with eosinophilic infiltration of the lamina propria and muscularis mucosae. There was no evidence of re...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract:BACKGROUND & AIMS:Gastroenteropancreatic neuroendocrine cells secrete chemical messengers in a calcium-dependent fashion. So far, other second messenger systems involved in regulated secretion have gained little attention. The aim of this study was to characterize guanosine 3',5'-cyclic monophosphate (cGMP)-mediated ve...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70593-1
更新日期:1998-04-01 00:00:00