Demonstration of tumor necrosis factor-alpha in preovulatory follicular fluid: its association with serum 17 beta-estradiol and progesterone.

Abstract:

:In the present study we have measured the concentrations of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in preovulatory follicular fluid (FF) samples obtained from patients undergoing ovulation induction with human menopausal gonadotropin/human chorionic gonadotropin. In 13 of the 24 (54%) FF samples obtained from 20 patients, TNF-alpha was detected. In contrast, IL-1 beta was observed in none of the 16 samples assessed. In the samples with detectable levels of TNF-alpha, the mean concentration was 40.1 pg/ml (range 20-74 pg/ml). In addition, we found an association between the presence of TNF-alpha in the FF and the serum level of 17 beta-estradiol (E2) and progesterone (P). The E2 and P levels were significantly lower in patients with detectable levels of TNF-alpha in FF than in patients with no detectable TNF-alpha in FF. In summary, the present study shows the presence of TNF-alpha and the absence IL-1 beta in hyperstimulated human preovulatory FF. In addition, our results show an association between the presence of TNF-alpha in FF and low E2 and P levels in serum, suggesting a role for TNF-alpha in the regulation of human steroidogenesis.

journal_name

Gynecol Obstet Invest

authors

Punnonen J,Heinonen PK,Teisala K,Kujansuu E,Jansen CT,Punnonen R

doi

10.1159/000294853

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

80-4

issue

2

eissn

0378-7346

issn

1423-002X

journal_volume

33

pub_type

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