Abstract:
:Conditions were developed for the reproducible production, isolation and characterization of a novel microbial extracellular polysaccharide believed to be involved in transient viscous bulking at an industrial wastewater treatment plant. The exopolysaccharide was extracted from cell-free culture supernatants of Thauera sp. strain MZ1T grown on a minimal medium with succinate. The purified polymer was found to be approximately 260 kDa in size by gel-permeation chromatography. The GC-MS analysis of the alditol acetate and per-O-trimethylsilyl methyl glycoside derivatives revealed that the exopolysaccharide was composed of four monosaccharides including: rhamnose, galacturonic acid, N-acetylglucosamine and N-acetylfucosamine. Glucose, which also appeared at low levels, is most likely from a co-eluting glucan. The FTIR and NMR spectroscopic analyses further revealed the presence of esterified component groups on the polymer. These results represent the first published description of a polysaccharide from a member of the genus Thauera, and lay the foundation for a deeper understanding of the factors potentially involved in zoogloeal cluster formation and viscous bulking.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Allen MS,Welch KT,Prebyl BS,Baker DC,Meyers AJ,Sayler GSdoi
10.1111/j.1462-2920.2004.00615.xkeywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
780-90issue
8eissn
1462-2912issn
1462-2920pii
EMI615journal_volume
6pub_type
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