Synthesis of (-)-longithorone A: using organic synthesis to probe a proposed biosynthesis.

abstract：:As assessed by ultrastructure, histochemical staining, and T-cell dependency, in vitro-differentiated inter-leukin 3-dependent mouse mast cells are comparable to the mast cells that reside in the gastrointestinal mucosa but not in the skin or the serosal cavity of the mouse. We now demonstrate that when cloned interle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dayton ET,Pharr P,Ogawa M,Serafin WE,Austen KF,Levi-Schaffer F,Stevens RL

更新日期：1988-01-01 00:00:00

abstract：:Copper homeostasis is essential for bacterial pathogen fitness and infection, and has been the focus of a number of recent studies. In Salmonella, envelope protection against copper overload and macrophage survival depends on CueP, a major copper-binding protein in the periplasm. This protein is also required to deliv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pezza A,Pontel LB,López C,Soncini FC

更新日期：2016-10-11 00:00:00

abstract：:The molecular circadian clocks in the mammalian retina are locally synchronized by environmental light cycles independent of the suprachiasmatic nuclei (SCN) in the brain. Unexpectedly, this entrainment does not require rods, cones, or melanopsin (OPN4), possibly suggesting the involvement of another retinal photopigm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buhr ED,Yue WW,Ren X,Jiang Z,Liao HW,Mei X,Vemaraju S,Nguyen MT,Reed RR,Lang RA,Yau KW,Van Gelder RN

更新日期：2015-10-20 00:00:00

abstract：:Human workers ensure the functioning of governments around the world. The efficacy of human workers, in turn, is linked to the climatic conditions they face. Here we show that the same weather that amplifies human health hazards also reduces street-level government workers' oversight of these hazards. To do so, we emp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Obradovich N,Tingley D,Rahwan I

更新日期：2018-08-28 00:00:00

abstract：:Fungi must recognize plant-specific signals to initiate subsequent morphogenetic events such as filamentation that lead to infection. Here we show that the plant hormone indoleacetic acid (IAA) induces adhesion and filamentation of Saccharomyces cerevisiae. Genome expression profiling of cells treated with IAA identif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prusty R,Grisafi P,Fink GR

更新日期：2004-03-23 00:00:00

abstract：:This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao W,Liu W,Christensen T,Zalutsky MR,Chilkoti A

更新日期：2010-09-21 00:00:00

abstract：:We have determined the molecular genetic basis of congenital adrenal hyperplasia due to 21-hydroxylase (21-OHase) deficiency. This common disorder of cortisol biosynthesis is HLA-linked. The haplotype HLA-(A3);Bw47;DR7 is strongly associated with 21-OHase deficiency and always carries a null allele at the locus encodi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PC,New MI,Dupont B

更新日期：1984-12-01 00:00:00

abstract：:Phase separation of multivalent protein and RNA molecules underlies the biogenesis of biomolecular condensates such as membraneless organelles. In vivo, these condensates encompass hundreds of distinct types of molecules that typically organize into multilayered structures supporting the differential partitioning of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boeynaems S,Holehouse AS,Weinhardt V,Kovacs D,Van Lindt J,Larabell C,Van Den Bosch L,Das R,Tompa PS,Pappu RV,Gitler AD

更新日期：2019-04-16 00:00:00

abstract：:An electron density map at 3 angstrom resolution has been calculated for Cu2+, Zn2+ superoxide dismutase from bovine erythrocytes, and the course of the main chain has been traced. The dominant structural feature is an 8-stranded barrel of antiparallel beta-pleated sheet. There is one very short helical section and tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richardson J,Thomas KA,Rubin BH,Richardson DC

更新日期：1975-04-01 00:00:00

abstract：:The blood-aqueous barrier plays a key role in regulating aqueous humor homeostasis by selectively restricting passage of proteins into the eye. The kinetics of aqueous flow are traditionally measured using artificial markers; however, these marker molecules do not address the barrier's selective permeability to plasma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu P,Thomson BR,Khalatyan N,Feng L,Liu X,Savas JN,Quaggin SE,Jin J

更新日期：2018-09-04 00:00:00

abstract：:A defined, minimal medium for the cellular slime mold Dictyostelium discoideum is reported. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franke J,Kessin R

更新日期：1977-05-01 00:00:00

abstract：:Mixed-metal oxides play a very important role in many areas of chemistry, physics, materials science, and geochemistry. Recently, there has been a strong interest in understanding phenomena associated with the deposition of oxide nanoparticles on the surface of a second (host) oxide. Here, scanning tunneling microscop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park JB,Graciani J,Evans J,Stacchiola D,Ma S,Liu P,Nambu A,Sanz JF,Hrbek J,Rodriguez JA

更新日期：2009-03-31 00:00:00

abstract：:Serum amyloid A (SAA) is an acute-phase plasma protein that functions in innate immunity and lipid homeostasis. SAA is a protein precursor of reactive AA amyloidosis, the major complication of chronic inflammation and one of the most common human systemic amyloid diseases worldwide. Most circulating SAA is protected f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jayaraman S,Gantz DL,Haupt C,Gursky O

更新日期：2017-08-08 00:00:00

abstract：:Focal electric stimulation of the auditory cortex is well suited for exploration of the function of the corticofugal (descending) system and the neural mechanism of plasticity in the central auditory system, because it evokes changes in frequency-tuning, called best frequency (BF) shifts, as does auditory fear conditi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Z,Suga N

更新日期：2005-12-27 00:00:00

abstract：:During the analysis of liver protein expression in the offspring of male mice irradiated with fission-spectrum neutrons, one offspring displayed a heritable 50% decrease in the abundance of two proteins. Homozygous mice lacking detectable quantities of these proteins were obtained through breeding. Characterization of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champion KM,Cook RJ,Tollaksen SL,Giometti CS

更新日期：1994-11-22 00:00:00

abstract：:Echoviruses have been implicated in multiple human disease syndromes, including aseptic meningitis, paralysis, and heart disease, but no animal model is available for studying the pathogenesis of infection. Production of human integrin very late antigen 2, a receptor for echovirus type 1, in transgenic mice conferred ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes SA,Thaker HM,Racaniello VR

更新日期：2003-12-23 00:00:00

abstract：:It is believed that neural representations of recent experiences become reactivated during sleep, and that this process serves to stabilize associated memories in long-term memory. Here, we initiated this reactivation process for specific memories during slow-wave sleep. Participants studied 50 object-location associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Dongen EV,Takashima A,Barth M,Zapp J,Schad LR,Paller KA,Fernández G

更新日期：2012-06-26 00:00:00

abstract：:Antibodies may bind to bacterial pathogens or their toxins to control infections, and their effector activity is mediated through the recruitment of complement component C1q or the engagement with Fcγ receptors (FcγRs). For bacterial pathogens that rely on a single toxin to cause disease, immunity correlates with toxi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Shi M,Tong X,Kim HK,Wang LX,Schneewind O,Missiakas D

更新日期：2020-09-15 00:00:00

abstract：:The polyomavirus enhancer is composed of multiple DNA sequence elements serving as binding sites for proteins present in mouse nuclear extracts that activate transcription and DNA replication. We have identified three such proteins and their binding sites and correlate them with enhancer function. Mutation of nucleoti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martin ME,Piette J,Yaniv M,Tang WJ,Folk WR

更新日期：1988-08-01 00:00:00

abstract：:A light form of DNA ligase (EC 6.5.1.2), the only form present in oocytes of the axolotl (Ambystoma mexicanum), has been shown to be replaced by a heavy form of the enzyme when the egg enters cleavage. This early biochemical event has been assumed to rely on direct nuclear input. Sucrose gradient analysis permits disc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Signoret J,David JC,Lefresne J,Houillon C

更新日期：1983-06-01 00:00:00

abstract：:Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis is a hallmark of major depressive disorder. A number of studies have shown that this dysregulation is correlated with impaired forebrain glucocorticoid receptor (GR) function. To determine whether a primary, acquired deficit in forebrain GR signaling is an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyle MP,Brewer JA,Funatsu M,Wozniak DF,Tsien JZ,Izumi Y,Muglia LJ

更新日期：2005-01-11 00:00:00

abstract：:A class II (B) general transcription factor of 34 kDa has been purified from HeLa cells to apparent homogeneity. This factor appears to be transcription factor IIB (TFIIB), since it binds in vitro to template-committed preinitiation complexes formed between a template containing the TATA box/cap-site elements of the a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moncollin V,Fischer L,Cavallini B,Egly JM,Chambon P

更新日期：1992-01-01 00:00:00

abstract：:Dimension reduction methods are commonly applied to high-throughput biological datasets. However, the results can be hindered by confounding factors, either biological or technical in origin. In this study, we extend principal component analysis (PCA) to propose AC-PCA for simultaneous dimension reduction and adjustme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin Z,Yang C,Zhu Y,Duchi J,Fu Y,Wang Y,Jiang B,Zamanighomi M,Xu X,Li M,Sestan N,Zhao H,Wong WH

更新日期：2016-12-20 00:00:00

abstract：:A serpin was identified in normal mammary gland by differential cDNA sequencing. In situ hybridization has detected this serpin exclusively in the myoepithelial cells on the normal and noninvasive mammary epithelial side of the basement membrane and thus was named myoepithelium-derived serine proteinase inhibitor (MEP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao G,Liu YE,Gentz R,Sang QA,Ni J,Goldberg ID,Shi YE

更新日期：1999-03-30 00:00:00

abstract：:The medial preoptic area has been shown to be intricately involved in many behaviors, including locomotion, sexual behavior, maternal care, and aggression. The gene encoding estrogen receptor-α (ERα) protein is expressed in preoptic area neurons, and a very dense immunoreactive field of ERα is found in the preoptic re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribeiro AC,Musatov S,Shteyler A,Simanduyev S,Arrieta-Cruz I,Ogawa S,Pfaff DW

更新日期：2012-10-02 00:00:00

abstract：:RS67333 is a partial serotonin subtype 4 receptor (5-HT4R) agonist that has been widely studied for its procognitive effect. More recently, it has been shown that its ability to promote the nonamyloidogenic cleavage of the precursor of the neurotoxic amyloid-β peptide leads to the secretion of the neurotrophic protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lecoutey C,Hedou D,Freret T,Giannoni P,Gaven F,Since M,Bouet V,Ballandonne C,Corvaisier S,Malzert Fréon A,Mignani S,Cresteil T,Boulouard M,Claeysen S,Rochais C,Dallemagne P

更新日期：2014-09-09 00:00:00

abstract：:The AG dinucleotide at the 3' splice sites of metazoan nuclear pre-mRNAs plays a critical role in catalytic step II of the splicing reaction. Previous studies have shown that replacement of the guanine by adenine in the AG (AG --> GG) inhibits this step. We find that the second step was even more severely inhibited by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaur RK,Beigelman L,Haeberli P,Maniatis T

更新日期：2000-01-04 00:00:00

abstract：:Selective blockade of gene expression by designed small molecules is a fundamental challenge at the interface of chemistry, biology, and medicine. Transcription factors have been among the most elusive targets in genetics and drug discovery, but the fields of chemical biology and genetics have evolved to a point where...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kushal S,Lao BB,Henchey LK,Dubey R,Mesallati H,Traaseth NJ,Olenyuk BZ,Arora PS

更新日期：2013-09-24 00:00:00

abstract：:Bordetella pertussis, the causative agent of whooping cough, secretes several toxins implicated in this disease. One of these putative virulence factors is the adenylate cyclase (AC) toxin that elevates intracellular cAMP in eukaryotic cells to cytotoxic levels. This toxin is a bifunctional protein comprising both AC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross MK,Au DC,Smith AL,Storm DR

更新日期：1992-06-01 00:00:00

abstract：:Staphylococcus aureus surface protein SasG promotes cell-cell adhesion during the accumulation phase of biofilm formation, but the molecular basis of this interaction remains poorly understood. Here, we unravel the mechanical properties of SasG on the surface of living bacteria, that is, in its native cellular environ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Formosa-Dague C,Speziale P,Foster TJ,Geoghegan JA,Dufrêne YF

更新日期：2016-01-12 00:00:00