Human embryonic stem cells form embryoid bodies containing visceral endoderm-like derivatives.

Abstract:

OBJECTIVE:The objective of this study was to determine the potential of human embryonic stem (hES) cells to provide an in vitro model of human extraembryonic endoderm development. METHODS:The hES cell line HES-2 was propagated in Dulbecco's modification of Eagle's medium (DMEM) in the presence of 20% fetal calf serum (FCS) on a mouse embryonic fibroblast feeder layer. Clumps of approximately 50-100 cells were transferred to fresh DMEM and FCS and grown as embryoid bodies (EBs) in suspension culture. EBs were subjected to immunohistochemistry for endodermal, ectodermal and mesodermal specific markers and immunoreactivity analysed by confocal microscopy and on cryosections. RESULTS:HES-2 cells reproducibly formed spherical EBs after 2-3 days in suspension culture as clumps. EBs continued to expand and by 7 days had commenced cavitation in a highly reproducible and organised fashion. Culture periods longer than 10 days led to cystic structures displaying inconsistent morphological organisation. Immunolocalisation of anti-alpha-fetoprotein-, anti-neurofilament- and anti-CD31-specific antibodies at 7 days of culture provided evidence of regional differentiation of endodermal, ectodermal and mesodermal derivatives in cavitating EBs. Further, of 10 cavitating EBs analysed at 7 days of culture, all displayed immunolocalisation of anti-pan-keratin-, anti-CK8- and anti-alpha-fetoprotein-specific antibodies to a peripheral cellular layer, suggestive of yolk sac visceral endoderm (VE) formation. CONCLUSION:Cavitation, the presence of regionalised cell lineage-specific immunoreactivity and the development of a VE-like peripheral cell layer demonstrate that the HES-2 hES cell line can be induced to undergo EB formation and provide scope to study early human primitive endoderm and yolk sac VE development.

journal_name

Fetal Diagn Ther

authors

Conley BJ,Trounson AO,Mollard R

doi

10.1159/000076701

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

218-23

issue

3

eissn

1015-3837

issn

1421-9964

pii

76701

journal_volume

19

pub_type

杂志文章
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    abstract:OBJECTIVE:To analyze the evolution of the management of delivery and neonatal care in a population of children with correctable malformations born in Parisian maternity hospitals during the period 1985-1994. METHODS:Data were collected by the Paris Registry of Congenital Anomalies from 400,000 births recorded in Paris...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000264471

    authors: De Vigan C,Goujard J,Vodovar V,Uzan S

    更新日期:1997-07-01 00:00:00

  • A case of fetal complete heart block recorded by magnetocardiography, ultrasonography and direct fetal electrocardiography.

    abstract::Fetal magnetocardiograms (FMCGs) were recorded in a case of fetal complete heart block (CHB) from the 30th to the 37th week of gestation using the multichannel SQUID system (Hitachi, Japan). M-mode ultrasonography and direct fetal electrocardiography using needle electrodes revealed fetal CHB. We identified independen...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000053878

    authors: Hosono T,Chiba Y,Shinto M,Miyashita S,Muramaki K,Kandori A,Tsukada K

    更新日期:2001-01-01 00:00:00

  • Bidirectional communication between neural and cardiac cells in human amniotic fluid.

    abstract:OBJECTIVE:To evaluate whether amniotic fluid cells contain cardiomyocyte- and neuron-like cells. STUDY DESIGN:In this experimental study, cells from human amniotic fluid samples were analyzed for mRNA expression of microtubule-associated protein 2 (MAP-2), vimentin and oxytocin (OT) receptor via RT-PCR. Immunocytochem...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000200016

    authors: Stefanidis K,Loutradis D,Anastasiadou V,Dinopoulou V,Eleni E,Arampatzi E,Lekka K,Mesogitis S,Antsaklis A

    更新日期:2009-01-01 00:00:00

  • Fetal Stomach Position Predicts Neonatal Outcomes in Isolated Left-Sided Congenital Diaphragmatic Hernia.

    abstract:INTRODUCTION:We sought to determine the relationship between the degree of stomach herniation by antenatal sonography and neonatal outcomes in fetuses with isolated left-sided congenital diaphragmatic hernia (CDH). MATERIALS AND METHODS:We retrospectively reviewed neonatal medical records and antenatal sonography of f...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000440649

    authors: Basta AM,Lusk LA,Keller RL,Filly RA

    更新日期:2016-01-01 00:00:00

  • Fetal heart disease: severity, associated anomalies and parental decision.

    abstract:OBJECTIVE:This study describes the association between the complexity of congenital cardiac and extracardiac malformations, and the parental decision of pregnancy continuation or termination. METHODS:Congenital heart defects (CHD) was diagnosed by ultrasound in 251 fetuses before the 24th week (23 + 6 weeks) of gestat...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000346564

    authors: Nell S,Wijngaarde CA,Pistorius LR,Slieker M,ter Heide H,Manten GT,Freund MW

    更新日期:2013-01-01 00:00:00

  • Risk estimation of intraamniotic infection development after serial amniocentesis.

    abstract::Microbial invasion and growth in amniotic fluid play an important role in infectious perinatal morbidity and mortality. In order to determine the influence of amniocentesis (ACT) on intra-amniotic infection development, we performed a study of 239 complete microbiological examinations of amniotic fluid specimens obtai...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000263904

    authors: Terzić MM,Plećas DV,Stimec BV,Petković SV

    更新日期:1994-01-01 00:00:00

  • Placental immaturity, endocardial fibroelastosis and fetal hypoxia.

    abstract::We describe a term newborn who, after a normal gestational course, presented at birth with absent cardiac activity and no spontaneous breathing. Death occurred within 30 h. Autopsy revealed placental villous immaturity, multiple acute hypoxic lesions, but also chronic hypoxic lesions like endocardial fibroelastosis. T...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000238105

    authors: Perez MH,Boulos T,Stucki P,Cotting J,Osterheld MC,Di Bernardo S

    更新日期:2009-01-01 00:00:00

  • Use of an Amnioport to Maintain Amniotic Fluid Volume in Fetuses with Oligohydramnios Secondary to Lower Urinary Tract Obstruction or Fetal Renal Anomalies.

    abstract:OBJECTIVE:We describe a technique to maintain amniotic fluid in fetuses with severe oligo-/anhydramnios secondary to lower urinary tract obstruction or fetal renal disease when urine production is inadequate to maintain a normal amniotic fluid volume (AFV). METHODS:An amnioport was inserted into the amniotic space. Th...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000445946

    authors: Polzin WJ,Lim FY,Habli M,Van Hook J,Minges M,Jaekle R,Crombleholme TM

    更新日期:2017-01-01 00:00:00

  • Fetoscopic laser photocoagulation of feeding vessels to a large placental chorioangioma following fetal deterioration after amnioreduction.

    abstract::Large placental chorioangiomas (>4 cm) can precipitate severe polyhydramnios, fetal anemia, growth restriction, high-output cardiac failure, hydrops, and fetal demise. We report a case of a large chorioangioma that was treated in a stepwise fashion with amnioreduction to ameliorate maternal discomfort, followed by fet...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000331944

    authors: Jones K,Tierney K,Grubbs BH,Pruetz JD,Detterich J,Chmait RH

    更新日期:2012-01-01 00:00:00

  • Meconium staining and meconium aspiration syndrome. Is there seasonal variation?

    abstract:OBJECTIVE:To determine whether the incidence of pregnancies complicated by meconium-stained amniotic fluid (MSAF) or meconium aspiration syndrome (MAS) differs with seasonal changes. METHODS:An established perinatal database was used to identify all term (> or = 37 weeks) singleton gestations resulting in a live birth...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000053911

    authors: Blackwell SC,Carreno CA,Hassan SS,Moldenhauer J,Wolfe HM,Berry SM,Kruger M,Sorokin Y

    更新日期:2001-07-01 00:00:00

  • Two-Dimensional Ultrasound Evaluation of the Fetal Cerebral Aqueduct: Improving the Antenatal Diagnosis and Counseling of Aqueductal Stenosis.

    abstract:OBJECTIVE:To describe a technique for the visualization and measurement of cerebral aqueduct diameter through a 2D sagittal median plane, and to report its aspect and measurement in fetuses with aqueductal stenosis (AS). METHODS:This was a cross-sectional study of 207 morphologically normal fetuses in low-risk pregnan...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000458439

    authors: Viñals F,Ruiz P,Quiroz G,Guerra FA,Correa F,Martínez D,Puerto B

    更新日期:2017-01-01 00:00:00

  • Understanding the Parent Perspective on Prenatal Consultation for Fetuses with Congenital Anomalies.

    abstract:INTRODUCTION:An increasing number of congenital anomalies are diagnosed prenatally. Understanding the implications of these diagnoses on the life of their child is critical for parents, and the surgical consultation is often the primary venue for this education. Little is known about the parent perspective on prenatal ...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000479504

    authors: Berman L,Costanzo C,Smola S,Taggi-Pinto A,Luks FL

    更新日期:2018-01-01 00:00:00

  • Is Cisterna Magna Width a Useful First-Trimester Marker of Aneuploidy?

    abstract:OBJECTIVE:To assess whether the cisterna magna (CM) width measured in first-trimester fetuses is a useful marker for aneuploidy detection. METHODS:This was a prospective study in 2 different cohorts in a tertiary referral center. The first cohort comprised 913 fetuses from the general pregnancy population during the p...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000503386

    authors: Pauta M,Grande M,Borobio V,Illanes C,Soler A,Nogué L,Borrell A

    更新日期:2020-01-01 00:00:00

  • Self-Amputation of the Extra Digit in a Fetus with Polydactyly: First Ultrasound Demonstration.

    abstract::We herein report the first ultrasound evidence of the self-amputation of an extra digit in case of fetal polydactyly. The prenatal evidence of fetal polydactyly is not always followed by postnatal confirmation. This is not always due to ultrasound misdiagnosis, but often to an in utero self-amputation phenomenon. We d...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000464462

    authors: Volpe N,Franchi L,Mazzone E,Migliavacca C,Raboni S,Percesepe A,Kaihura CT,Frusca T,Ghi T

    更新日期:2017-01-01 00:00:00

  • Management of twin anemia-polycythemia sequence using intrauterine blood transfusion for the donor and partial exchange transfusion for the recipient.

    abstract::Twin anemia-polycythemia sequence (TAPS) is a rare condition which may occur either spontaneously in uncomplicated monochorionic twin pregnancies or may develop after laser treatment in twin-twin transfusion syndrome. TAPS is characterized by a large intertwin discordance in hemoglobin levels without discordance in am...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000346413

    authors: Genova L,Slaghekke F,Klumper FJ,Middeldorp JM,Steggerda SJ,Oepkes D,Lopriore E

    更新日期:2013-01-01 00:00:00

  • A study to determine if human umbilical cord hematopoietic stem cells can survive in baboon extra-embryonic celomic fluid: a prerequisite for determining the feasibility of in-utero stem cell xeno-transplantation via celocentesis.

    abstract:OBJECTIVES:To determine if: (1) human umbilical cord stem cells could survive for 20 h in extra-embryonic celomic fluid obtained at 40 days of development from baboon pregnancies by ultrasound-guided celocentesis and, (2) human hematopoietic stem cell survival could be enhanced by adding increasing concentrations of he...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000097112

    authors: Santolaya-Forgas J,Galan I,Deleon-Luis J,Wolf R

    更新日期:2007-01-01 00:00:00

  • Antibodies to trophoblast antigens HLA-G, placenta growth factor, and neuroD2 do not improve detection of circulating trophoblast cells in maternal blood.

    abstract:OBJECTIVES:Non-invasive prenatal diagnosis using circulating fetal trophoblast cells has been challenging due to lack of a reproducible trophoblast-specific antibody. We investigated the use of three trophoblast cell-specific antibodies, HLA-G, placenta growth factor, and neuroD2, for the isolation of trophoblast cells...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000097102

    authors: Tjoa ML,Delli-Bovi L,Johnson KL,Bianchi DW

    更新日期:2007-01-01 00:00:00

  • Mass Effect Alone May Not Explain Pulmonary Vascular Pathology in Severe Congenital Diaphragmatic Hernia.

    abstract::Congenital diaphragmatic hernia (CDH) and congenital pulmonary airway malformation (CPAM) are diseases in which chest-occupying lesions can result in severe pulmonary hypoplasia. However, significant postnatal mortality due to pulmonary hypertension (PH) is more often seen in patients with CDH. We analyzed prenatal ec...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000434643

    authors: Derderian SC,Jayme CM,Cheng LS,Keller RL,Moon-Grady AJ,MacKenzie TC

    更新日期:2016-01-01 00:00:00

  • Perinatal Outcome and Long-Term Neurodevelopment after Intracranial Haemorrhage due to Fetal and Neonatal Alloimmune Thrombocytopenia.

    abstract:OBJECTIVES:To evaluate the perinatal and long-term neurodevelopmental outcome in a cohort of children with intracranial haemorrhage (ICH) due to fetal and neonatal alloimmune thrombocytopenia (FNAIT) and to clearly outline the burden of this disease. SUBJECTS AND METHODS:We performed an observational cohort study and ...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000488280

    authors: Winkelhorst D,Kamphuis MM,Steggerda SJ,Rijken M,Oepkes D,Lopriore E,van Klink JMM

    更新日期:2019-01-01 00:00:00

  • Ominous normalization of middle cerebral artery flow velocity waveforms preceding fetal death: case report.

    abstract::The loss of middle cerebral artery compensatory vasodilation appears to be ominous in fetuses with absent end-diastolic umbilical waveforms. The authors report a case with a loss of the 'brain-sparing effect' 24 h before fetal death. Current pathophysiological explanations are discussed. ...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000264215

    authors: Chitrit Y,Zorn B,Filidori M,Bucourt M,Chasseray JE,Caubel P

    更新日期:1995-03-01 00:00:00

  • Calprotectin in Serially Collected Meconium Portions as a Biomarker for Intrauterine Fetal Environment.

    abstract:OBJECTIVE:Understanding the pathomechanisms underlying high meconium calprotectin concentrations is the key to the potential uses of this parameter for the assessment of the intrauterine environment in which the fetus develops. The aim of this study was to measure calprotectin concentrations in serial meconium portions...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000472150

    authors: Lisowska-Myjak B,Skarżyńska E,Żytyńska-Daniluk J

    更新日期:2018-01-01 00:00:00

  • Lower extremity neuromotor function and short-term ambulatory potential following in utero myelomeningocele surgery.

    abstract:OBJECTIVE:To evaluate lower extremity neuromotor function (LENF) and short-term ambulatory potential following fetal myelomeningocele (fMMC) closure. METHODS:Retrospective chart review of 54 children that underwent fMMC closure at our institution prior to the NIHCD-MOMS trial. Neonatal LENF was compared to predicted f...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000197359

    authors: Danzer E,Gerdes M,Bebbington MW,Sutton LN,Melchionni J,Adzick NS,Wilson RD,Johnson MP

    更新日期:2009-01-01 00:00:00

  • The early amniocentesis study: a randomized clinical trial of early amniocentesis and midtrimester amniocentesis. II. Evaluation of procedure details and neonatal congenital anomalies.

    abstract::The present study provides detailed neonatal and congenital malformation follow-up from 695 women enrolled in a prospective randomized multicenter study comparing the safety and accuracy of early (11-12 weeks of gestation) and midtrimester amniocentesis (15-16 weeks of gestation). No differences were found for total p...

    journal_title:Fetal diagnosis and therapy

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1159/000264440

    authors: Wilson RD,Johnson J,Windrim R,Dansereau J,Singer J,Winsor EJ,Kalousek D

    更新日期:1997-03-01 00:00:00

  • Rate and Outcomes of Pulmonary Stenosis and Functional Pulmonary Atresia in Recipient Twins with Twin-Twin Transfusion Syndrome.

    abstract:OBJECTIVE:To evaluate the rate of pulmonary stenosis and functional pulmonary atresia (PS/PA) in recipient twins prior to fetal surgery for twin-twin transfusion syndrome (TTTS) and their pre- and postnatal outcomes. METHODS:We carried out a prospective study including 260 cases of TTTS. Echocardiography was performed...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000448075

    authors: Ortiz JU,Masoller N,Gómez O,Bennasar M,Eixarch E,Lobmaier SM,Crispi F,Gratacos E,Martinez JM

    更新日期:2017-01-01 00:00:00

  • Detection of DeltaF508del using quantitative real-time PCR, comparison of the results obtained by fluorescent PCR.

    abstract:OBJECTIVE:Cystic fibrosis (CF) is the most common autosomal recessive genetic disorder in the Caucasian population. The molecular diagnosis is difficult since there are about 1,000 mutations in the CF transmembrane regulator gene. The DeltaF508del is the cause of the CF in 64% of the cases in Hungary. Our aim was to co...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000095846

    authors: Nagy B,Nagy GR,Lázár L,Bán Z,Papp Z

    更新日期:2007-01-01 00:00:00

  • Maternal mortality following diagnostic 2nd-trimester amniocentesis.

    abstract::We present 2 cases of maternal mortality after transabdominal amniocentesis performed during the 2nd trimester of pregnancy. In both these cases, blood cultures revealed Escherichia coli. Broad-spectrum intravenous antibiotic treatment started immediately after admission to the hospital did not change the rapid progre...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000075150

    authors: Elchalal U,Shachar IB,Peleg D,Schenker JG

    更新日期:2004-03-01 00:00:00

  • Prenatal diagnosis of 18q-syndrome: a case of fetal mosaicism with a normal karyotype in chorionic villi.

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    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000317012

    authors: Anselem O,Bazin A,Mechler C,Blin G,Garel C,Aboura A,Moutard ML,Mandelbrot L

    更新日期:2010-01-01 00:00:00

  • Perinatal outcome of monochorionic twins with selective intrauterine growth restriction and different types of umbilical artery Doppler under expectant management.

    abstract:OBJECTIVES:To evaluate the prognosis of monochorionic twins with selective intrauterine growth restriction (sIUGR), classified according to the type of umbilical artery Doppler, under expectant management. METHODS:The outcome of 81 cases with isolated sIUGR was evaluated according to a classification based on umbilica...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000253880

    authors: Ishii K,Murakoshi T,Takahashi Y,Shinno T,Matsushita M,Naruse H,Torii Y,Sumie M,Nakata M

    更新日期:2009-01-01 00:00:00

  • Sonographic screening for trisomy 21: fetal humerus:foot length ratio, a useful new marker.

    abstract::We have analyzed morphometric measurements from midgestational fetal necropsies and shown that arm and foot lengths are linear relationships versus gestational age (GA). Using foot length as the GA determinant, we found that the ratio of arm:foot length was also a linear relationship and was decreased in trisomy 21 fe...

    journal_title:Fetal diagnosis and therapy

    pub_type: 临床试验,杂志文章

    doi:10.1159/000263920

    authors: Johnson MP,Michaelson JE,Barr M Jr,Treadwell MC,Isada NB,Dombrowski MP,Pryde PG,Addis J,Evans MI

    更新日期:1994-03-01 00:00:00

  • Prenatal diagnosis of X-linked ichthyosis using molecular cytogenetics.

    abstract::A case is presented in which X-linked ichthyosis was diagnosed prenatally using fluorescence in situ hybridization. Fetal sex was known by second trimester ultrasound in a woman with very low second trimester MSUE3. All of the 15 maternal peripheral blood metaphase spreads examined displayed two hybridization signals ...

    journal_title:Fetal diagnosis and therapy

    pub_type: 杂志文章

    doi:10.1159/000264422

    authors: Santolaya-Forgas J,Cohen L,Vengalil S,Field F,Rodriguez A,McCorquadale M,McCorquadale DJ

    更新日期:1997-01-01 00:00:00