Bidirectional communication between neural and cardiac cells in human amniotic fluid.

Abstract:

OBJECTIVE:To evaluate whether amniotic fluid cells contain cardiomyocyte- and neuron-like cells. STUDY DESIGN:In this experimental study, cells from human amniotic fluid samples were analyzed for mRNA expression of microtubule-associated protein 2 (MAP-2), vimentin and oxytocin (OT) receptor via RT-PCR. Immunocytochemistry was also performed with MAP-2, OT receptor, vimentin and troponin I antibodies. RESULTS:In all samples, MAP-2, vimentin and mRNA expression were detectable. OT receptor was also detectable. The cells showed strong immunoreactivity for molecular markers of neurogenic cells including MAP-2 and vimentin. The cells also showed strong immunoreactivity for molecular markers of cardiac muscle such as troponin I and for OT receptors. This report also shows that atosiban (an OT antagonist) added to culture medium of amniotic fluid cells did not induce neurogenic and cardiomyogenic differentiation. CONCLUSIONS:The observed concurrent development of cardiomyocyte- and neuron-like cells suggests that amniotic fluid contains progenitor cells and there is bidirectional communication between both cell types.

journal_name

Fetal Diagn Ther

authors

Stefanidis K,Loutradis D,Anastasiadou V,Dinopoulou V,Eleni E,Arampatzi E,Lekka K,Mesogitis S,Antsaklis A

doi

10.1159/000200016

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

62-6

issue

1

eissn

1015-3837

issn

1421-9964

pii

000200016

journal_volume

25

pub_type

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