Abstract：
:Many models of Parkinson's disease (PD) have succeeded in replicating dopaminergic neuron loss or alpha-synuclein aggregation but not the formation of classical Lewy bodies, the pathological hallmark of PD. Our cybrid model of sporadic PD was created by introducing the mitochondrial genes from PD patients into neuroblastoma cells that lack mitochondrial DNA. Previous studies using cybrids have shown that information encoded by mitochondrial DNA in patients contributes to many pathogenic features of sporadic PD. In this paper, we report the generation of fibrillar and vesicular inclusions in a long-term cybrid cell culture model that replicates the essential antigenic and structural features of Lewy bodies in PD brain without the need for exogenous protein expression or inhibition of mitochondrial or proteasomal function. The inclusions generated by PD cybrid cells stained with eosin, thioflavin S, and antibodies to alpha-synuclein, ubiquitin, parkin, synphilin-1, neurofilament, beta-tubulin, the proteasome, nitrotyrosine, and cytochrome c. Future studies of these cybrids will enable us to better understand how Lewy bodies form and what role they play in the pathogenesis of PD.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Trimmer PA,Borland MK,Keeney PM,Bennett JP Jr,Parker WD Jrdoi
10.1046/j.1471-4159.2003.02168.xkeywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
800-12issue
4eissn
0022-3042issn
1471-4159pii
2168journal_volume
88pub_type
杂志文章abstract：:We examined the effects of treatments affecting norepinephrine release on the number of norepinephrine reuptake recognition sites as reflected by desipramine binding. To do this, we used manipulations having similar presynaptic but contrasting postsynaptic effects. Presynaptic inhibition by 6-hydroxydopamine lesion or...
journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1994.63041386.x
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.2004.02975.x
更新日期：2005-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.2007.04902.x
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abstract：:NNC 01-0012, a second-generation benzazepine compound, pharmacologically differentiates multiple vertebrate D1 receptor subtypes (D1A, D1B, D1C, and D1D) and displays high selectivity and affinity for dopamine D1C receptors. Functionally, whereas NNC 01-0012 acts as a full or poor antagonist at D1C and D1A receptor-me...
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journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.2008.05265.x
更新日期：2008-06-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.2008.05347.x
更新日期：2008-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
