Abstract:
:Nuclei of mature mammalian spermatozoa are extraordinarily resistant to chemical and thermal injury. Additionally, decondensation of spermatozoa DNA can be accompanied by little or no visual changes of the sperm head. This study tested whether human spermatozoa could be recovered following several cycles of primer extension preamplification (PEP) and used to achieve fertilization and subsequent development of human oocytes. An attempt was also made to amplify PEP buffer after spermatozoon removal. The results demonstrate that the sperm head can be successfully recovered following treatment with KOH or proteinase K followed by one to four cycles of PEP. It is also shown that following this treatment, the spermatozoa can be injected into the oocytes and will transform into a pronucleus if the oocyte is activated by sperm cytosolic fraction. In some cases, it was also possible to obtain polymerase chain reaction signals using a buffer after sperm cells were removed following several cycles of PEP. Although sperm participation in development was confirmed by fluorescence in-situ hybridization, light microscopy revealed some degree of damage to spermatozoal chromosomes. It is concluded that pre-conceptual analysis of sperm cells may be possible, but more research is necessary to determine the optimal conditions that would preserve sperm DNA integrity while allowing accurate diagnoses.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Dozortsev D,Serafim R,Cardoso JJ,Abdelmassih S,Nagy P,Diamond MP,Abdelmassih Rdoi
10.1016/s1472-6483(10)61882-2keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
392-9issue
4eissn
1472-6483issn
1472-6491pii
S1472-6483(10)61882-2journal_volume
7pub_type
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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更新日期:2004-05-01 00:00:00
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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更新日期:2021-01-01 00:00:00
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journal_title:Reproductive biomedicine online
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pub_type: 杂志文章
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
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更新日期:2008-10-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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journal_title:Reproductive biomedicine online
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