Abstract：
:Conventional PCR-based genotyping is useful for forensic testing but cannot be used to determine parental origins of alleles in DNA specimens. Here we describe a novel method of combined conventional genotyping and PIA typing (parentally imprinted allele typing) at a minisatellite region upstream from the H19 locus. The PIA typing uses two sets of primers and DNA digested with methylation-sensitive Hha I enzyme. The first amplification produces only the methylated fragment of paternal H19 allele, and the second detects polymorphism in the minisatellite. Hence, this distinguishes paternal and maternal alleles by difference in the DNA methylation. Furthermore, the polymorphism in this polymorphic locus was examined using 199 unrelated Japanese and 171 unrelated Germans, their polymorphism information content being 0.671 and 0.705, respectively. Feasibility of this typing is demonstrated for six families, and the usefulness is shown by application to paternity testing.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Naito E,Dewa K,Fukuda M,Sumi H,Wakabayashi Y,Umetsu K,Yuasa I,Yamanouchi Hkeywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
1275-9issue
6eissn
0022-1198issn
1556-4029journal_volume
48pub_type
杂志文章abstract：:A case of apparent suicide by injection of snake venom was investigated. Evidence found at the scene and presumably used for self-injection was positive for nonspecific venom but had the characteristics of a viper venom. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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更新日期：2005-05-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：10.1111/j.1556-4029.2006.00070.x
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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doi：10.1111/j.1556-4029.2007.00475.x
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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