Abstract：
:Mitragyna speciosa (MS), a plant commonly known as kratom, is a widely used "legal high" opiate alternative for pain relief. DNA extracted from MS and 26 additional plant species was amplified by PCR using primers targeting the strictosidine beta-D-glucosidase (SGD) and secologanin synthase 2 (SLS2) genes and detected by high-resolution melt curves using three intercalating dyes. Amplicon sizes were confirmed using agarose gel electrophoresis. The observed melt temperatures for SGD and SLS2 were 77.08 ± 0.38°C and 77.61 ± 0.46°C, respectively, using SYBR® Green I; 80.18 ± 0.27°C and 80.59 ± 0.08°C, respectively, using Radiant™ Green; and 82.19 ± 0.04°C and 82.62 ± 0.13°C, respectively, using the LCGreen® PLUS dye. The SLS2 primers demonstrated higher specificity and identified MS DNA at 0.05 ng/μL. In a duplex reaction, SLS2 and tetrahydrocannabinoic acid synthase gene primers detected and differentiated MS and Cannabis sativa (CS) by melt peaks at 82.63 ± 0.35°C and 85.58 ± 0.23°C, respectively, using LCGreen® PLUS.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Cowan AF,Elkins KMdoi
10.1111/1556-4029.14167subject
Has Abstractpub_date
2020-01-01 00:00:00pages
52-60issue
1eissn
0022-1198issn
1556-4029journal_volume
65pub_type
杂志文章abstract：:A population study on the distribution of alleles for the D1S80 locus (pMCT118) was carried out on 141 unrelated and healthy blood donors from the province of Messina (Eastern Sicily). Forty-two different genotypes and 16 different alleles were observed and two of these were found to be relatively common in the sample...
journal_title：Journal of forensic sciences
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abstract：:The scientific community and the judicial system are different components of society with different structures and functions. Nevertheless, science can contribute relevant and useful information to judicial deliberations if the inherent limitations of that information are understood. These limitations stem from the wa...
journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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doi：
更新日期：2004-11-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：
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journal_title：Journal of forensic sciences
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更新日期：2013-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12881
更新日期：2016-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00262.x
更新日期：2006-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：10.1111/j.1556-4029.2009.01109.x
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journal_title：Journal of forensic sciences
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doi：
更新日期：1986-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12184
更新日期：2013-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：10.1111/1556-4029.13103
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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doi：
更新日期：1992-05-01 00:00:00
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
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