A method for the isolation of root hairs from the model legume Medicago truncatula.

abstract：:Four glutathione S-transferase (GST) genes, NbGSTU1, NbGSTU2, NbGSTU3, and NbGSTF1, were amplified from cDNA of Nicotiana benthamiana leaves infected with Colletotrichum destructivum using primers based on conserved regions of N. tabacum GST sequences. Expression of NbGSTU1 and NbGSTU3 increased progressively during i...

journal_title：Journal of experimental botany

authors： Dean JD,Goodwin PH,Hsiang T

更新日期：2005-06-01 00:00:00

abstract：:Witches' broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant-fungus interface, the apoplastic flui...

journal_title：Journal of experimental botany

authors： Barau J,Grandis A,Carvalho VM,Teixeira GS,Zaparoli GH,do Rio MC,Rincones J,Buckeridge MS,Pereira GA

更新日期：2015-03-01 00:00:00

abstract：:Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably express...

journal_title：Journal of experimental botany

authors： Guénin S,Mauriat M,Pelloux J,Van Wuytswinkel O,Bellini C,Gutierrez L

更新日期：2009-01-01 00:00:00

abstract：:It has been suggested that antioxidants play a role in regulating or modulating senescence dynamics of plant tissues. Ethylene has been shown to promote early plant senescence while controlled atmospheres (CA; reduced O2 levels and elevated CO2 levels) can delay its onset and/or severity. In order to examine the possi...

journal_title：Journal of experimental botany

authors： Hodges DM,Forney CF

更新日期：2000-03-01 00:00:00

abstract：:Lift-off velocity may be the most useful surrogate to measure the secondary dispersal capacity of diaspores. However, the most important diaspore attribute determining diaspore lift-off velocity is unclear. Furthermore, it is not known whether terminal velocity used to characterize the primary dispersal capacity of di...

journal_title：Journal of experimental botany

authors： Liang W,Liu Z,Liu M,Qin X,Baskin CC,Baskin JM,Xin Z,Wang Z,Su Z,Zhou Q

更新日期：2020-07-06 00:00:00

abstract：:In grafted plants, rootstocks assure the mineral nutrition of the scion and modify its development. In this study, we show that two grapevine rootstock genotypes have different shoot branching architectures when cultivated as cuttings and that this trait is transmitted to the scion when grafted. Shoot branching plasti...

journal_title：Journal of experimental botany

authors： Cochetel N,Météier E,Merlin I,Hévin C,Pouvreau JB,Coutos-Thévenot P,Hernould M,Vivin P,Cookson SJ,Ollat N,Lauvergeat V

更新日期：2018-07-18 00:00:00

abstract：:Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana...

journal_title：Journal of experimental botany

authors： Gutla PV,Boccaccio A,De Angeli A,Gambale F,Carpaneto A

更新日期：2012-10-01 00:00:00

abstract：:The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the...

journal_title：Journal of experimental botany

authors： Huang C,He W,Guo J,Chang X,Su P,Zhang L

更新日期：2005-12-01 00:00:00

abstract：:The rootless, aquatic Utricularia species belong to the largest and most cosmopolitan carnivorous plant genus. Populations of Utricularia plants are an important component of many standing, nutrient-poor, and humic waters. Carbon (C) allocation is an aspect of Utricularia's ecophysiology that has not been studied prev...

journal_title：Journal of experimental botany

authors： Sirová D,Borovec J,Santrůcková H,Santrucek J,Vrba J,Adamec L

更新日期：2010-01-01 00:00:00

abstract：:Shipping of peaches to distant markets and storage require low temperature; however, cold storage affects fruit quality causing physiological disorders collectively termed 'chilling injury' (CI). In order to ameliorate CI, different strategies have been applied before cold storage; among them heat treatment (HT) has b...

journal_title：Journal of experimental botany

authors： Lara MV,Borsani J,Budde CO,Lauxmann MA,Lombardo VA,Murray R,Andreo CS,Drincovich MF

更新日期：2009-01-01 00:00:00

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental processes. Indole-3-acetic acid (IAA) and abscisic acid (ABA) play critical roles in developmental programmes and environmental responses, respectively, through complex signalling and metabolism networks. However, crosstalk betwe...

journal_title：Journal of experimental botany

authors： Du H,Wu N,Fu J,Wang S,Li X,Xiao J,Xiong L

更新日期：2012-11-01 00:00:00

abstract：:During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. In contrast to Rht-B1b and Rht-D1b, the Rht-B1c allele is characterized by extreme dwarfism and exceptional...

journal_title：Journal of experimental botany

authors： Van De Velde K,Chandler PM,Van Der Straeten D,Rohde A

更新日期：2017-01-01 00:00:00

abstract：:Our current understanding of how plants move auxin through their tissues is largely built on the use of polar auxin transporter inhibitors. Although the most important proteins that mediate auxin transport and its regulation have probably all been identified and the mapping of their interactions is well underway, mech...

journal_title：Journal of experimental botany

authors： Teale W,Palme K

更新日期：2018-01-04 00:00:00

abstract：:At least seven isoforms (PsABI3-1 to PsABI3-7) of a putative, pea ABI3-like factor, originated by alternative splicing, have been identified after cDNA cloning. A similar variability had previously only been described for monocot genes. The full-length isoform, PsABI3-1, contains the typical N-terminal acidic domains ...

journal_title：Journal of experimental botany

authors： Gagete AP,Riera M,Franco L,Rodrigo MI

更新日期：2009-01-01 00:00:00

abstract：:The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxid...

journal_title：Journal of experimental botany

authors： Rodríguez AA,Maiale SJ,Menéndez AB,Ruiz OA

更新日期：2009-01-01 00:00:00

abstract：:Sugars play an important role in grapevine flowering. This complex process from inflorescence initiation to fruit maturity takes two growing seasons. Currently, most of the available data concern the involvement of sugars as energy sources during the formation of reproductive structures from initiation of inflorescenc...

journal_title：Journal of experimental botany

authors： Lebon G,Wojnarowiez G,Holzapfel B,Fontaine F,Vaillant-Gaveau N,Clément C

更新日期：2008-01-01 00:00:00

abstract：:During the summer, evaporative demand at midday often exceeds the transport capacity of most desert plants. However, date palms maintain their ecological dominance with sustained and uniquely high rates of transpiration. This high rate of flow cannot be attributed to soil water supply alone. In order to quantify intra...

journal_title：Journal of experimental botany

authors： Sperling O,Shapira O,Schwartz A,Lazarovitch N

更新日期：2015-01-01 00:00:00

abstract：:Numerous studies have revealed the extent of genetic and phenotypic variation between both species and cultivars of tomato. Using a series of tomato lines resulting from crosses between a cherry tomato and three independent large fruit cultivar (Levovil, VilB, and VilD), extensive profiling of both central primary met...

journal_title：Journal of experimental botany

authors： Zanor MI,Rambla JL,Chaïb J,Steppa A,Medina A,Granell A,Fernie AR,Causse M

更新日期：2009-01-01 00:00:00

abstract：:Several root anatomical phenes affect water acquisition from drying soil, and may therefore have utility in breeding more drought-tolerant crops. Anatomical phenes that reduce the metabolic cost of the root cortex ('cortical burden') improve soil exploration and therefore water acquisition from drying soil. The best e...

journal_title：Journal of experimental botany

authors： Lynch JP,Chimungu JG,Brown KM

更新日期：2014-11-01 00:00:00

abstract：:Cereal grain germination is central for plant early development, and efficient germination has a major role in crop propagation and malting. Endosperm starch is the prime energy reserve in germination and seedling establishment. In this study, it was hypothesized that optimized starch granule structure, and not only t...

journal_title：Journal of experimental botany

authors： Shaik SS,Carciofi M,Martens HJ,Hebelstrup KH,Blennow A

更新日期：2014-06-01 00:00:00

abstract：:Leaf senescence is a programmed developmental process governed by various endogenous and exogenous factors, such as the plant developmental stage, leaf age, phytohormone levels, darkness, and exposure to stresses. It was found that, in addition to its well-documented role in the enhancement of plant frost tolerance, o...

journal_title：Journal of experimental botany

authors： Sharabi-Schwager M,Lers A,Samach A,Guy CL,Porat R

更新日期：2010-01-01 00:00:00

abstract：:Plasma membranes were isolated using the aqueous polymer two-phase partition method from the algae Chara corallina and Chara longifolia, algae which differ in their ability to grow in saline environments. Enrichment of plasma membrane and depletion of tonoplast relative to the microsomal fraction was monitored using p...

journal_title：Journal of experimental botany

authors： Faraday CD,Spanswick RM,Bisson MA

更新日期：1996-04-01 00:00:00

abstract：:Ca(2+) acts as an important cellular second messenger in eukaryotes. In both plants and animals, a wide variety of environmental and developmental stimuli trigger Ca(2+) transients of a specific signature that can modulate gene expression and metabolism. In animals, mitochondrial energy metabolism has long been consid...

journal_title：Journal of experimental botany

authors： Wagner S,De Bortoli S,Schwarzländer M,Szabò I

更新日期：2016-06-01 00:00:00

abstract：:Mutants sensitive to ionizing radiation in yeast and mammals include an assortment of DNA repair genes. The majority of these DNA repair genes are involved in the repair of DNA double-strand breaks. In this study a forward genetic screen is used to identify gamma-sensitive mutants of Arabidopsis thaliana. The gamma-pl...

journal_title：Journal of experimental botany

authors： Hefner E,Preuss SB,Britt AB

更新日期：2003-02-01 00:00:00

abstract：:The two-fold purpose of this work is, first, to review current hypotheses for multiple paths of sugar-sensing in an oxygen-responsive context, and second, to present evidence for the extent of sugar/oxygen overlap regulating genes for sucrose and ethanol metabolism. Current data indicate that sugar signals in plants m...

journal_title：Journal of experimental botany

authors： Koch KE,Ying Z,Wu Y,Avigne WT

更新日期：2000-02-01 00:00:00

abstract：:Exposure to high ambient levels of NaCl affects plant water relations and creates ionic stress in the form of the cellular accumulation of Cl- and, in particular, Na+ ions. However, salt stress also impacts heavily on the homeostasis of other ions such as Ca2+, K+, and NO(3)(-) and therefore requires insights into how...

journal_title：Journal of experimental botany

authors： Maathuis FJ

更新日期：2006-01-01 00:00:00

abstract：:Drivers behind food security and crop protection issues are discussed in relation to food losses caused by pests. Pests globally consume food estimated to feed an additional one billion people. Key drivers include rapid human population increase, climate change, loss of beneficial on-farm biodiversity, reduction in pe...

journal_title：Journal of experimental botany

authors： E Birch AN,Begg GS,Squire GR

更新日期：2011-06-01 00:00:00

abstract：:Cultivated varieties generally differ greatly from wild genotypes of the same closely related species. However, the processes responsible for these differences have not been elucidated. To analyse variations in fruit mass, fruit growth was characterized in a peach cultivar, a wild related species non-cultivated, and f...

journal_title：Journal of experimental botany

authors： Quilot B,Génard M,Kervella J,Lescourret F

更新日期：2002-07-01 00:00:00

abstract：:Rapid changes in turgor pressure (P:) and temperature (T:) are giving new information about the mechanisms of plant growth. In the present work, single internode cells of the large-celled alga Chara corallina were used as a model for plant growth. P was changed without altering the chemical environment of the wall whi...

journal_title：Journal of experimental botany

authors： Proseus TE,Zhu GL,Boyer JS

更新日期：2000-09-01 00:00:00

abstract：:Cytophotometry of individual nuclei was used to examine the level of endoreduplication in epidermal cells from the upper and lower parts of the ovary during Ornithogalum umbellatum flower and fruit development. An increase in DNA content from 2-4C to 2-8C in both parts of the ovary was observed, while the epidermal ce...

journal_title：Journal of experimental botany

authors： Kwiatkowska M,Poplonska K,Kazmierczak A,Stepinski D,Rogala K,Polewczyk K

更新日期：2007-01-01 00:00:00