Abstract:
:msec7-1, a mammalian homologue of yeast sec7p, is known as a GDP/GTP exchange factor (GEF) for the ADP ribosylation factor (ARF) family of small GTPases. Here, we report that msec7-1 overexpression in cultured Aplysia neurons leads to an extensive neuritogenesis in a GEF activity-dependent manner through the modulation of the actin cytoskeleton. Similarly, the overexpression of ARNO, another mammalin GEF, produces extensive neuritogenesis in Aplysia neurons. In addition, msec7-1 overexpression increases the number of varicosities with an altered size and shape in a GEF activity-dependent manner. The overexpression of msec7-1 in pre-synaptic sensory neurons co-cultured with post-synaptic target motor neurons leads to an increase in the amplitude of the excitatory post-synaptic potential through its GEF activity. Our results demonstrate that msec7-1 regulates neuritogenesis and synaptic transmission.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Huh M,Han JH,Lim CS,Lee SH,Kim S,Kim E,Kaang BKdoi
10.1046/j.1471-4159.2003.01718.xkeywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
282-5issue
1eissn
0022-3042issn
1471-4159pii
1718journal_volume
85pub_type
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