Cochlear mitochondrial DNA3867bp deletion in aged mice.

Abstract:

OBJECTIVES:To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice. METHODS:We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing. RESULTS:mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001). CONCLUSION:Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Zhang X,Han D,Ding D,Dai P,Yang W,Jiang S,Salvi RJ

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

1390-3

issue

9

eissn

0366-6999

issn

2542-5641

journal_volume

115

pub_type

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