Abstract:
BACKGROUND:Previous studies have suggested that primary degeneration of hair cells causes secondary degeneration of spiral ganglion neurons (SGNs), but the effect of SGN degeneration on hair cells has not been studied. In the adult mouse inner ear ouabain can selectively and permanently induce the degeneration of type 1 SGNs while leaving type 2 SGNs, efferent fibers, and sensory hair cells relatively intact. This study aimed to investigate the dynamic changes in hair cell ribbon synapse induced by loss of SGNs using ouabain application to the round window niche of adult mice. METHODS:In the analysis, 24 CBA/CAJ mice aged 8-10 weeks, were used, of which 6 normal mice were used as the control group. After ouabain application in the round window niche 6 times in an hour, ABR threshold shifts at least 30 dB in the three experimental groups which had six mice for 1-week group, six for 1-month group, and six for 3-month group. All 24 animals underwent function test at 1 week and then immunostaining at 1 week, 1 month, and 3 months. RESULTS:The loss of neurons was followed by degeneration of postsynaptic specializations at the afferent synapse with hair cells. One week after ouabain treatment, the nerve endings of type 1 SGNs and postsynaptic densities, as measured by Na/K ATPase and PSD-95, were affected but not entirely missing, but their partial loss had consequences for synaptic ribbons that form the presynaptic specialization at the synapse between hair cells and primary afferent neurons. Ribbon numbers in inner hair cells decreased (some of them broken and the ribbon number much decreased), and the arrangement of the synaptic ribbons had undergone a dynamic reorganization: ribbons with or without associated postsynaptic densities moved from their normal location in the basal membrane of the cell to a more apical location and the neural endings alone were also found at more apical locations without associated ribbons. After 1 month, when the neural postsynaptic densities had completed their degeneration, most ribbons were lost and the remaining ribbons had no contact with postsynaptic densities; after 3 months, the ribbon synapses were gone except for an occasional remnant of a CtBP2-positive vesicle. Hair cells were intact other than the loss of ribbons (based on immunohistochemistry and DPOAE). CONCLUSION:These findings define the effect of SGN loss on the precise spatiotemporal size and location of ribbons and the time course of synaptic degeneration and provide a model for studying plasticity and regeneration.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Yuan Y,Chi Fsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
1941-6issue
10eissn
0366-6999issn
2542-5641journal_volume
127pub_type
杂志文章abstract::Klinefelter’s syndrome (KS) is the most common sex chromosome disease in men. Classical features of the syndrome include a eunuchoidal body habitus, small testes and hypergonadotrophic hypogonadism. There has been an increased risk of diabetes mellitus and autoimmune disease for KS patients. This paper reports a case ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Chronic liver disease causes aberrant formation of fibrous tissue that impedes normal liver function, ultimately resulting in liver cirrhosis. Iron uptake can occur within the hepatic parenchyma or within the various nodules that form in a cirrhotic liver, termed siderotic nodules (SN). We aimed to investiga...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
abstract::The association of gluckinase (GCK) gene with type 2 (non-insulin-dependent) diabetes mellitus was investigated in 168 Chinese subjects (85 unrelated type 2 diabetics and 83 non-diabetic controls). The microsatellite polymorphism marker, GCK-5', was amplified with polymerase chain reaction. Four alleles were observed ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The effects of anisodamine on acetylcholine-induced endothelium-dependent relaxation were studied in organ chambers and under bioassay conditions. In organ chamber experiments, both acetylcholine and bradykinin induced an endothelium-dependent relaxation of norepinephrine-contracted canine femoral arteries in a concen...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Renal angiomyolipoma is a type of benign tumor that occurs sporadically in addition to being associated with tuberous sclerosis. Preoperative embolization of large tumors is important to avoid excessive blood loss during surgery. We reported a patient with a 5505-g giant renal angiomyolipoma in a solitary kidney. The ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:The biofragmentable anastomosis ring (BAR) is a simple alternative device to create intestinal anastomosis. Our study was designed to evaluate the clinical value of BAR in intestinal anastomosis. METHODS:A total of 167 patients performed intestinal anastomosis from January 2002 to February 2006 were randomi...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2009-08-05 00:00:00
abstract:BACKGROUND:Cornea epithelial cells play early and crucial roles in the initiation of ocular surface responses to pathogens. Participation of toll-like receptor (TLR) 2 and TLR4, which are major forms of fungi receptors, may be involved in Aspergillus fumigatus induced immune responses. The objective of the present stud...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-03-05 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is a heterogeneous disease; current research relies on cancer cell lines and animal cancer models, which may not precisely imitate inner human tumors and guide clinical medicine. The purpose of our study was to explore and further improve the process of producing three-dimensional (3D...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.191782
更新日期:2016-10-20 00:00:00
abstract:BACKGROUND:Severe valgus deformity often has bone defect and laxity of the medial ligamentous, and total knee arthroplasty in severe valgus knee is, in most cases, more challenging for surgeons. The usefulness of a computer assisted navigation system in reestablishing the mechanical axis has been well established. Henc...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Precise evaluation of the live donor's liver is the most important factor for the donor's safety and the recipient's prognosis in living donor liver transplantation (LDLT). Our study assessed the clinical value of computer-assisted three-dimensional quantitative assessment and a surgical planning tool for do...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Vascular rings are uncommon anomalies in which preferred strategies for diagnosis and management may vary among institutions. In this study, we reported our approach and a review of our 5-year experience. METHODS:From May 2006 to April 2011, 45 children (31 boys) with vascular rings underwent surgical repai...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked recessive neuromuscular diseases resulting from dystrophin (DMD) gene mutations. It has been known that the carrier of DMD mutations may also have symptoms of the disease. While de novo mutation is quite common in BMD/DMD pati...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.215338
更新日期:2017-10-05 00:00:00
abstract:BACKGROUND:Airway mucus hypersecretion is an important pathophysiological feature of chronic obstructive pulmonary disease, which is closely associated with cigarette smoking. However, the signal transduction pathway from the cell surface to the nucleus through which cigarette smoke causes upregulation of mucin gene ex...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Congenital heart disease (CHD) is a common heart condition which does considerable harm to the health of children and adolescents. CHD epidemiological characteristics of Tibetan children whose ages ranged from 4 to 18 years were investigated in Qinghai Province. METHODS:A total of 32 578 Tibetan children, l...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-12-20 00:00:00
abstract:BACKGROUND:Cerebral ischemia-reperfusion injury is the main reason for the loss of neurons in the ischemic cerebrovascular disease. Therefore, to deeply understand its pathogenesis and find a new target is the key issue to be solved. This research aimed to investigate the neuroprotective effects of salvianolic acid B (...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Aquired immune deficiency syndrome (AIDS) presents a challenge to medical researchers because of its unique pathological and clinical picture. The clinical data, particularly autopsy evidence, from China have failed to provide enough pathological and etiological evidence for AIDS diagnosis, which impairs the...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-09-20 00:00:00
abstract:OBJECTIVE:To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig). METHODS:We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Serum expression of cytokines may provide information about the clinical outcome of advanced non-small cell lung cancer (NSCLC) patients. This study aimed to investigate the relationship between serum cytokine levels and the clinical outcome of erlotinib treatment in a second or third line setting in patient...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Irritable bowel syndrome (IBS) is reported associated with the alteration of gut microbial composition termed as dysbiosis. However, the pathogenic mechanism of IBS remains unclear, while the studies of Chinese individuals are scarce. This study aimed to understand the concept of dysbiosis among patients wit...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000192
更新日期:2019-04-20 00:00:00
abstract:BACKGROUND:To compare the efficacy and safety of hormone replacement therapy (HRT) combined with fluoxetine, with HRT alone, in post-menopausal women suffering from depression. METHODS:A randomized, open-label, parallel trial was applied. HRT was administered to all patients for 2 cycles, with 14 days of estrogen ther...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:To date, there have been no reports on altered nitric oxide (NO) content in ischemia/reperfusion with regard to in vivo preconditioning procedures. These studies are important for understanding the mechanisms of NO during early myocardial ischemic preconditioning. The aim of the present study was to investig...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-07-05 00:00:00
abstract::We presented the experimental results of endothelial cells (EC) seeding in small caliber interpositional dacron grafts of canine carotid arteries. Six weeks after surgery, 9 (56.25%) seeded, and 6 (37.5%) control grafts remained patent. The mean thrombus-free surface area was 83.4% in the seeded and 62% in the control...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract:BACKGROUND:Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diag...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:The change of glucose transporter 4 (GLUT4) expression could influence glucose uptake in the myocardial cells and then effect myocardial metabolism, which maybe one of the factor for the diabetes cardiovascular disease. This study aimed to explore the influence of glucose and insulin at different concentrati...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma especially prevailing among the natives of southern China. The regimen of concurrent chemoradiotherapy (CCRT) that include platinum and 5-fluorouracil (5-FU) is considered to be the standard treatment for NPC. However, its clinical use is limited by...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Dermoscopy is a useful technique for improving the diagnostic accuracy of various types of skin disorders. In China, dermoscopy has been widely accepted, and domestic researchers have made tremendous progress in the field of dermoscopy. The main purpose of this review is to summarize the current status of der...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.1097/CM9.0000000000000396
更新日期:2019-09-05 00:00:00
abstract::Anti-HNK-1 monoclonal antibody (MAb) was reactive with non-cancerous and cancerous prostatic epithelia, as well as natural killer cells, myelinated nerves and cells from APUD systems. The expression of HNK-1 antigen on prostate cancer was investigated immunohistochemically by avidinbiotin-peroxidase complex (ABC) meth...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND:Oxygen inhalation therapy is essential for the treatment of patients with chronic mountain sickness (CMS), but the efficacy of oxygen inhalation for populations at high risk of CMS remains unknown. This research investigated whether oxygen inhalation therapy benefits populations at high risk of CMS. METHODS...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:10.4103/0366-6999.182831
更新日期:2016-06-05 00:00:00
abstract:OBJECTIVE:To clarify the role of sexual related Y (SRY) gene detection in the diagnosis of gonadal dysgenesis. METHODS:Sixteen cases of gonadal dysgenesis were included in this study: 5 with androgen insensitivity syndrome, 1 with 17-alpha-hydroxylase deficiency, 4 with true hermaphrodite, 2 with 45, X/46, XY gonadal ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND:The potential benefit of arthroscopic surgery for osteoarthritic knee associated with medial meniscus tear is controversial. This study was conducted to determine the effect of pre-operative medial meniscus extrusion (MME) on arthroscopic surgery outcomes in the osteoarthritic knee associated with medial men...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000492
更新日期:2019-11-05 00:00:00