Abstract:
AIMS:As part of the UK antimicrobial resistance strategy and action plan, the Public Health Laboratory Service (PHLS) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. Most laboratories report urine Gram negative isolates, as "coliforms" according to morphological appearance, but without an acceptable identification system the antimicrobial surveillance data will be meaningless. Commercially available identification systems tend to be expensive and time consuming. Chromogenic agars, which claim to improve the detection of mixed cultures and identification of organisms from urine, have now become available and may provide a cost effective alternative. The primary aim of this study was to compare the performance of cystine lactose electrolyte deficient (CLED) agar with a chromogenic agar (Oxoid urinary tract infection medium; CUTI) in terms of isolation rates and ability to detect mixed cultures. Secondary aims were to evaluate the correlation of "presumptive" identification of isolates from chromogenic media with that of two commercial identification systems and to appraise the sensitivity of the semiquantitative loop and filter paper strip culture techniques. METHOD:One thousand, four hundred and sixty six urine samples were examined in four laboratories using the semiquantitative culture methods of 1 microl loop and filter paper strip. The degree of accuracy of organism identification was measured by comparing the presumptive identification using colony colour supplemented with simple bench tests, with identification obtained from two more complex commercial systems. RESULTS:There was no significant difference between the performance of the loop and filter paper strip methods on the CLED agar, but the CUTI agar performed significantly better than the CLED agar for the detection of significant isolates and mixed cultures. This difference was greater using the loop method. Identification of the organisms using the commercial systems gave > 99% agreement and was therefore considered suitable as a standard against which to compare the presumptive CUTI identification. Using the manufacturer's colony colour criteria in combination with a bench indole test, the CUTI medium was 99% specific for Escherichia coli, although this was reduced to 97% if the indole test was omitted. Citrobacter spp were the most commonly misidentified organisms, giving false presumptive identification as E coli. By testing oxidase activity to differentiate Pseudomonas spp and the absence of indole production to support the identification of Proteus mirabilis, the CUTI medium provided a suitable identification for 86.8% of Gram negative isolates. The remaining 13.2% would require further identification. CONCLUSION:CUTI medium improves the detection of mixed cultures, thereby improving the reliability of reporting of significant isolates when compared with CLED agar. When supplemented with simple bench tests it provides an identification system capable of speciating 86.8% of Gram negative isolates and providing a valuable cost effective mechanism for antimicrobial resistance surveillance.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Fallon D,Andrews N,Frodsham D,Gee B,Howe S,Iliffe A,Nye KJ,Warren REdoi
10.1136/jcp.55.7.524keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
524-9issue
7eissn
0021-9746issn
1472-4146journal_volume
55pub_type
杂志文章abstract:INTRODUCTION:Large granular lymphocyte (LGL) leukaemia is considered a mature T-cell or natural killer (NK) cell neoplasm, characterised by a clonal proliferation of LGL. AIMS:To analyse the characteristics and to establish (if possible) the prognostic parameters of these patients diagnosed in a single centre: Univers...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-207191
更新日期:2021-01-21 00:00:00
abstract::A case of squamous cell carcinoma of the terminal ileum with no underlying duplication or inflammatory disorder is described. The neoplasm seemed to have originated from the surface epithelium, invading the wall and metastasising to the regional lymph nodes. The 65 year old patient was free of disease three years afte...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.3.253
更新日期:1991-03-01 00:00:00
abstract::The detection of lymphoid cells by routine examination of the urine after renal allotransplantation has proved to be a useful early indication of rejection. In a study of 36 rejection episodes, 20 (56%) were associated with a significant number of lymphocytes in the urine. The incidence was much higher when rejection ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.2.99
更新日期:1970-03-01 00:00:00
abstract::Early epidemiological studies of cervical neoplasia suggested a causal relation with sexual activity and human papillomaviruses (HPVs) have emerged as prime suspects as venerally transmitted carcinogens. HPVs fall into two broad camps: low risk types, associated with cervical condylomas and CIN 1; and high risk types ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.51.2.96
更新日期:1998-02-01 00:00:00
abstract:AIMS:Congenital neutropenia (CN) is a rare inherited disease that results in recurrent, life-threatening bacterial infections due to a deficiency of mature neutrophils. They are usually caused by heterozygous ELANE mutations although mutations in other genes like HAX-1, G6PC3 and GFI1 have also been reported. Identifyi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206306
更新日期:2020-06-01 00:00:00
abstract::Routine anaerobic culture of urine identified the urinary tract as the primary focus of sepsis in a postoperative patient with Bacteroides fragilis septicaemia. Specimens of urine from six other symptomatic patients grew > 10(8) cfu/litre of a Bacteroides species in pure growth. The significance of these isolates is d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.9.709
更新日期:1998-09-01 00:00:00
abstract::Cytomegalovirus (CMV) is a recognised cause of morbidity and mortality in immunocompromised individuals. This review will concentrate on recent advances in the understanding of the complex interplay between the host and parasite and the pathological consequences of perturbation of the host immune system. The classic v...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.2.84
更新日期:2001-02-01 00:00:00
abstract:AIMS:To characterise further the proliferative compartment of the normal cervix and to document its alteration, if any, in the various grades of cervical intraepithelial neoplasia (CIN), particularly changes to the basal epithelial layer; to hypothesise as to the diagnostic and biological significance of any observed d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.8.667
更新日期:1996-08-01 00:00:00
abstract::Serum IgM, IgA, and IgG concentrations were measured in 19 patients suffering from blood-dependent homozygous beta-thalassaemia. The study was prospective and the same patients served as presplenectomy controls. IgG levels rose rapidly and continuously after splenectomy IgA levels also rose but not so sharply. IgM fel...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.6.546
更新日期:1978-06-01 00:00:00
abstract:AIMS:To assess the presence of carcinoma in situ (CIS) in patients with nasopharyngeal carcinoma (NPC) and to see if the number of biopsy sites facilitates detection of CIS. METHODS:Formalin fixed, paraffin wax embedded biopsy specimens (n = 285) from 187 patients with NPC in 1987 were studied for the presence of CIS ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.10.898
更新日期:1992-10-01 00:00:00
abstract:BACKGROUND/AIMS:In the natural history of gastric cancer, non-invasive neoplasia (NiN) precedes invasive carcinoma. A histological classification of gastric NiN has recently been proposed by a World Health Organisation international panel of experts. Genetic instability is known to be among the molecular pathways invol...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.025676
更新日期:2005-08-01 00:00:00
abstract::This report describes a case of mucinous carcinoma and Müllerian metaplasia arising within an appendiceal duplication cyst found incidentally during an emergency Caesarian section. Intestinal duplication cysts are rare and although there are occasional reports of malignant transformation, this is the first case where ...
journal_title:Journal of clinical pathology
pub_type: 信件
doi:10.1136/jcp.55.7.551
更新日期:2002-07-01 00:00:00
abstract::The pathological findings of an intradural and extramedullary cyst in the mid cervical spinal canal are described in a 55 year old woman who presented with a short history of pain and paraesthesia of the right arm. Intradural well defined solitary cystic lesions in the spinal canal are uncommon, their pathogenesis is ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.11.1032
更新日期:1992-11-01 00:00:00
abstract::Mast cells were identified by electronmicroscopy in the alveolar wall of the lung in 20 subjects (10 normal, 10 abnormal). A quantitative and qualitative study was made of the mast cells. In the normal lung there was an average concentration of 350 mast cells/mm2 of alveolar wall and in the abnormal 523/mm2. Mast cell...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1333
更新日期:1981-12-01 00:00:00
abstract::Acute myocardial disease, which may be associated with abnormal platelet activity, has been reported after routine immunisation. Thirty-two army apprentices undergoing immunisation were studied for changes in the heparin neutralising activity (HNA) of platelet poor plasma. HNA increased after immunisation. This increa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1152
更新日期:1979-11-01 00:00:00
abstract::Cervical smears from 327 women were examined using the polymerase chain reaction (PCR) targeted to a sequence in the Bam H1 W region of the Epstein-Barr virus (EBV) to determine the prevalence of the virus in the cervix. EBV was detected in 131 (40%) of the 327 women. Of the 235 women with normal cytology, 98 (42%) we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.1.92
更新日期:1994-01-01 00:00:00
abstract::Experience after 18 months' work with the SMA-4 system of multiple analysis for routine haematology is described, as well as the modifications to the machine which have been made for smoother operation. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.1.19
更新日期:1969-01-01 00:00:00
abstract::As part of a quality assurance programme at the Aga Khan University Medical Centre, Karachi, Pakistan, all hysterectomy specimens were reviewed from January 1986 to December 1989. Adenomyosis was found in 237 of the 419 (56.5%) specimens studied. Of these 237 patients, 232 (97.9%) were parous and 196 (82.8%) were in t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.10.817
更新日期:1990-10-01 00:00:00
abstract::A case of acute myelomonocytic leukaemia (AMMOL) is reported in which skin infiltration with xanthomatous nodules was the presenting feature. The histological, including ultrastructural, appearances are described. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.11.1200
更新日期:1982-11-01 00:00:00
abstract::A case of signet-ring cell lymphoma affecting bone marrow is reported. The tumour presented as multiple lytic lesions in the lumbosacral spine. A bone biopsy specimen showed the typical appearances of signet-ring cell lymphoma, and the cells stained positively with antiserum to CD20, though neither immunoglobulin ligh...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.2.184
更新日期:1994-02-01 00:00:00
abstract:AIM:To examine a model for the evaluation of appropriateness of testing in an emergency biochemistry laboratory. METHODS:A model was devised in which incoming emergency test requests were categorised as appropriate or inappropriate. Explicit criteria were used to define eight minor categories, which were chosen to ref...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.12.1126
更新日期:1995-12-01 00:00:00
abstract:AIMS:Dysfunctional prothrombin residue Arg596 associated mutation has been found to precipitate venous thromboembolism (VTE). In the current study we investigated the prevalence of Arg596 associated mutations in Chinese patients with VTE and explored the functional impact of Arg596Gln mutation on coagulation function i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2017-204888
更新日期:2018-07-01 00:00:00
abstract:AIMS:Neoadjuvant chemoradiation therapy (CRT) is an increasingly used therapeutic strategy for rectal cancer. Clinically, it remains a major challenge to predict therapeutic response and patient outcome after CRT. Rsf-1 (HBXAP), a novel nuclear protein with histone chaperon function, mediates ATPase-dependent chromatin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-200786
更新日期:2012-08-01 00:00:00
abstract:AIMS:To investigate treatment with glycerol/washing as a potential substitute for freeze-thawing in the production of leucocyte depleted red cell concentrates for patients with a history of non-haemolytic reactions following transfusion. METHODS:The standard procedure of treatment with glycerol/-80 degrees C freezing/...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.8.742
更新日期:1993-08-01 00:00:00
abstract:AIMS:To assess the significance of flat epithelial atypia (FEA) on core biopsy by evaluating the proportion of cases upgraded to in-situ and invasive carcinoma on further sampling with vacuum-assisted biopsy (VAB) or diagnostic surgical biopsy. METHODS:A retrospective analysis was performed of all core biopsies contai...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200176
更新日期:2011-11-01 00:00:00
abstract::Enteroviruses are rare causes of acute focal encephalitis. A fatal case of echovirus type 9 infection is reported in a 9 month old boy who presented with a fever and a macular rash followed by two focal seizures. Echovirus type 9 was isolated from lung tissue after seven days. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.9.865
更新日期:1993-09-01 00:00:00
abstract::Numerous clinical pathology departments are deploying or planning to deploy digital pathology systems for all or part of their diagnostic output. Digital pathology is an evolving technology, and it is important that departments uphold or improve on current standards. Leeds Teaching Hospitals NHS Trust has been scannin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206319
更新日期:2020-07-01 00:00:00
abstract::A case of Cryptococcus neoformans meningitis is described in an HIV negative patient with undiagnosed systemic sarcoidosis. The patient presented with signs of meningitis together with generalised lymphadenopathy and hepatosplenomegaly. Cryptococcal meningitis was diagnosed on lumbar puncture. She was treated with int...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.12.928
更新日期:1999-12-01 00:00:00
abstract::Carcinoma-associated fibroblast (CAF) as prominent cell type of the tumour microenvironment has complex interaction with both the cancer cells and other non-neoplastic surrounding cells. The CAF-derived regulators and extracellular matrix proteins can support cancer progression by providing a protective microenvironme...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2014-202561
更新日期:2014-12-01 00:00:00
abstract::Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). Ther...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.6.475
更新日期:1999-06-01 00:00:00