Abstract：
:We have generated new sensors for the specific detection and studies of bioavailability of metals by engineering Pseudomonas fluorescens with reporter gene systems. One broad host range mercury (pTPT11) and two arsenite (pTPT21 and pTPT31) sensor plasmids that express metal presence by luminescence phenotype were constructed and transferred into Escherichia coli DH5a and Pseudomonas fluorescens OS8. The maximal induction was reached after 2 h of incubation in metal solutions at room temperature (22 degrees C). In optimized conditions the half maximal velocity of reaction was achieved at acidic pH using a d-luciferin substrate concentration that was nearly sixfold lower for P. fluorescens OS8 than for E. coli DH5a. When using a luciferin concentration (150 mM) that was optimal for E. coli the luminescence declined rapidly in the case of Pseudomonas, for which the substrate level 25 mM gave a stable reading between about 20 min and 3 h. The ability of the strain OS8 to quantitatively detect specific heavy metals in spiked soil and soil extracts is as good, or even better in being a real-time reporter system, than that of a traditional chemical analysis. The Pseudomonas strain used is an isolate from pine rhizosphere in oil and heavy metal contaminated soil. It is also a good humus soil colonizer and is therefore a good candidate for measuring soil heavy metal bioavailability.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Petänen T,Virta M,Karp M,Romantschuk Mdoi
10.1007/s002480000095keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
360-368issue
4eissn
0095-3628issn
1432-184Xjournal_volume
41pub_type
杂志文章abstract：:By using substrate molecules of varying degrees of surface activity, we were able to measure some features of bacterial activity in the surface microlayers (SM) and in the subsurface (bulk) water. The fraction of active cells was determined by a combined microautoradiography-epifluorescence (ME) method. Measurements w...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02019021
更新日期：1983-12-01 00:00:00
abstract：:Genetically engineeredErwinia carotovora persisted significantly longer in thermally perturbed microcosms (35 days) than in nonstressed microcosms (5 days). Decreased pressure of competitors and predators and increased nutrient availability were examined as the most probable reasons for greater vulnerability of pertur...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02543863
更新日期：1990-12-01 00:00:00
abstract：:Lichens host highly diverse microbial communities, with bacteria being one of the most explored groups in terms of their diversity and functioning. These bacteria could partly originate from symbiotic propagules developed by many lichens and, perhaps more commonly and depending on environmental conditions, from differ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-020-01662-y
更新日期：2021-01-06 00:00:00
abstract：:Growth kinetics of 2Nitrobacter strains (N.w. and L) coexisting in the same soil are studied in batch and continuous culture. Monod's parameters are estimated numerically from experimental data in the case of the batch experiment, and from steady-state equations in the case of the chemostat. In both cases, the 2 strai...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02011417
更新日期：1984-06-01 00:00:00
abstract：:Methanotrophic bacteria are able to use methane (CH4) as a sole carbon and energy source. Photochemical oxidation of methane takes place in the stratosphere, whereas in the troposphere, this process is carried out by methanotrophic bacteria. On the one hand, it is known that the efficiency of biological CH4 oxidation ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-018-1248-3
更新日期：2019-04-01 00:00:00
abstract：:The phospholipid fatty acid (PLFA) profiles of soil microbial communities were determined in relation to the patterns of tree cover in a mature Norway spruce plantation. Replicate samples of the surface organic layers were taken close to the trunk, at 1 m and at 2 m (under the edge of the canopy) beneath five trees. S...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-001-0006-z
更新日期：2001-10-01 00:00:00
abstract：:The aim of the study was to assess the effect of the mycorrhizosphere of A. tortillis spp. raddiana mycorrhized with Glomus intraradices on the root nodulation by Sinorhizobium terangae (ORS 1009) and/or Mesorhizobium plurifarium (ORS 1096) in two different culture substrates (sandy soil and sand). The endomycorrhizal...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-002-1022-3
更新日期：2003-02-01 00:00:00
abstract：:Cell growth and division of the ciliateTetrahymena corlissi were examined upon refeeding after prolonged starvation of up to 12 days. Division did not automatically occur when a certain critical cell size was reached. Rather, it varied both with the nutritional history of the cell and the nutrient conditions in which ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02011248
更新日期：1987-03-01 00:00:00
abstract：:The community of culturable cellulolytic bacteria was analyzed in two long-term experimental field sites on Albic Luvisol (silty sand) and Haplic Phaeozem (loam), with and without farmyard manure treatment. Against the backdrop of significant differences in soil properties, the bacterial community structure differed c...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9296-0
更新日期：2008-04-01 00:00:00
abstract：:Mediterranean forests own distinct characteristics resulting from climate, soil, and vegetation that affect soil microbial communities' assembly and their associated functions. We initiated a multi-scalar analysis of environmental drivers of soil functioning to (1) identify pertinent factorial scales and (2) determine...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-020-01518-5
更新日期：2020-10-01 00:00:00
abstract：:Geosmithia spp. (Ascomycota: Hypocreales) are little-studied, dry-spored fungi that occur in galleries built by many phloeophagous bark beetles. This study mapped the distribution and environmental preferences of Geosmithia species occurring in galleries of temperate European bark beetles. One hundred seven host tree ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9251-0
更新日期：2008-01-01 00:00:00
abstract：:The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9587-8
更新日期：2010-02-01 00:00:00
abstract：:Actinobacteria are widely distributed in the marine environment. To date, few studies have been performed to explore the coral-associated Actinobacteria, and little is known about the diversity of coral-associated Actinobacteria. In this study, the actinobacterial diversity associated with one soft coral Alcyonium gra...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-013-0205-4
更新日期：2013-07-01 00:00:00
abstract：:The relative amounts of hyphal inoculum in forest soils may determine the capacity for fungi to compete with and replace early colonizers of wood in ground contact. Our aim in this study was to test the flexibility of priority effects (colonization timing) by varying the timing of inoculum introduction (i.e., precolon...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0588-5
更新日期：2015-05-01 00:00:00
abstract：:Peatland testate amoebae (TA) are well-established bioindicators for depth to water table (DWT), but effects of hydrological changes on TA communities have never been tested experimentally. We tested this in a field experiment by placing Sphagnum carpets (15 cm diameter) collected in hummock, lawn and pool microsites ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-014-0367-8
更新日期：2014-05-01 00:00:00
abstract：:Montmorillonitic clay influences the biological control ofEscherichia coli in aquatic systems, the magnitude of the effects being dependent on the state of the clay and the type of host-antagonist interaction. The interaction ofBdellovibrio andE. coli was partially inhibited by the presence of montmorillonite. Because...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02013272
更新日期：1977-12-01 00:00:00
abstract：:The long-term disposal of radioactive wastes in a deep geological repository is the accepted international solution for the treatment and management of these special residues. The microbial community of the selected host rocks and engineered barriers for the deep geological repository may affect the performance and th...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0630-7
更新日期：2015-11-01 00:00:00
abstract：:Nasal swabs of 100 healthy dogs were obtained in 2011 in Tunisia and tested for Staphylococcus pseudintermedius recovery. Antimicrobial resistance profile and virulence gene content were determined. Multilocus-sequence-typing (MLST) and SmaI-pulsed-field gel electrophoresis (PFGE) were investigated. S. pseudintermediu...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-013-0243-y
更新日期：2013-08-01 00:00:00
abstract：:The depth-dependent, seasonal, and diel variability of virus numbers, dissolved DNA (D-DNA), and other microbial parameters was investigated in the northern Adriatic Sea. During periods of water stratification, we found higher virus abundances and virus/bacterium ratios (VBRs) as well as a larger variability of D-DNA ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00184511
更新日期：1995-07-01 00:00:00
abstract：:Airborne bacteria are important biological components of bioaerosol and play an important role in ecosystem. Bacteria at a high concentration in the atmosphere can result in biological air pollution and all kinds of diseases. In this study, a systematical survey on the culturable airborne bacteria was carried out for ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9216-3
更新日期：2007-10-01 00:00:00
abstract：:Septate endophytes proliferating in the roots of grasslands' plants shed doubts on the importance of arbuscular mycorrhizal (AM) symbioses in dry soils. The functionality and diversity of the AM symbioses formed in four replicates of three adjacent plant communities (agricultural, native, and restored) in Grasslands N...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9629-2
更新日期：2010-05-01 00:00:00
abstract：:The spatial distribution of the bacterial biomass and production and of potential heterotrophic activity (PHA) were measured every 4 h between 23 July (10:00 h) and 25 July (10:00 h) 1997 in a recently flooded oligo-mesotrophic reservoir (the Sep Reservoir, Puy-de-Dôme, France), in relation to temperature, the phytopl...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000056
更新日期：2000-12-01 00:00:00
abstract：:Qualitative and quantitative investigations were made on the luminous bacteria associated with the gut of pond cultured milk fishChanos chanos. Significant differences in luminous bacterial numbers were found between gut and pond water and between gut and pond sediment, but not between pond water and sediment. No sign...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02011207
更新日期：1986-06-01 00:00:00
abstract：:We studied the relationship between testate amoebae (Protozoa) communities and the depth to the water table (DWT), pH, conductivity, and microhabitat type in Sphagnum dominated peatlands of north-western Poland and built predictive (transfer function) models for inferring DWT and pH based on the testate amoebae commun...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-004-0105-8
更新日期：2005-07-01 00:00:00
abstract：:Understanding the pathogenic potential of a new pathogen strain or a known pathogen in a new locale is crucial for management of disease in both wild and farmed animals. The Ostreid herpesvirus-1 (OsHV-1), a known pathogen of early-life-stage Pacific oysters, Crassostrea gigas, has been associated with mortalities of ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-011-9937-1
更新日期：2012-04-01 00:00:00
abstract：:The colonization and succession of diazotrophs are essential for the development of organic soil layers in desert. We examined the succession of diazotrophs in the well-established revegetated areas representing a chronosequence of 0 year (control), 22 years (restored artificially since 1981), 57 years (restored artif...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0657-9
更新日期：2016-01-01 00:00:00
abstract：:Bacterial symbionts are increasingly recognised as mediators of ecologically important traits of their animal hosts, with acquisition of new traits possible by uptake of novel symbionts. The entomopathogenic nematode Heterorhabditis downesi associates with two bacterial symbionts, Photorhabdus temperata subsp. tempera...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0829-2
更新日期：2017-01-01 00:00:00
abstract：:Bacterial surface structures called pili have been studied extensively for their role as possible colonization factors. Most sequenced Vibrio genomes predict a variety of pili genes in these organisms, including several types of type IV pili. In particular, the mannose-sensitive hemagglutinin (MSHA) and the PilA pili,...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0021-2
更新日期：2012-08-01 00:00:00
abstract：:This study investigated the relationships of time-dependent hydrological variability and selected microbial food web components. Samples were collected monthly from the Kopački Rit floodplain in Croatia, over a period of 19 months, for analysis of bacterioplankton abundance, cell size and biomass; abundance of heterot...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0016-z
更新日期：2012-07-01 00:00:00
abstract：:A growing body of evidence now supports the isolation of microorganisms from ancient materials. However, questions about the stringency of extraction methods and the genetic relatedness of isolated organisms to their closest living relatives continue to challenge the authenticity of these ancient life forms. Previous ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-003-2016-5
更新日期：2004-07-01 00:00:00
