Abstract:
BACKGROUND & AIMS:Intrapancreatic activation of digestive enzymes is a key event in the parenchymal cell injury of pancreatitis. We hypothesized that neutrophils recruited to the pancreas during pancreatitis may contribute to such activation. METHODS:To cause experimental pancreatitis, rats and mice were treated with high doses of cerulein. Activation of the digestive enzyme, trypsin, was measured in pancreatic homogenates using a fluorogenic assay and localized immunocytochemically with antibody to trypsin-activation peptide (TAP). RESULTS:Compared with controls, rats depleted of neutrophils with antineutrophil serum exhibited a marked attenuation in intrapancreatic trypsin activation and acinar cell TAP labeling induced by high-dose cerulein. To examine the mechanism, mice deficient in either nicontinamide adenine dinucleotide phosphate (NADPH) oxidase, or myeloperoxidase (MPO) were studied for trypsin activation. Mice deficient in NADPH oxidase exhibited attenuation of the cerulein-induced trypsin activation, but those deficient in MPO did not. Using measurements of Western blot analysis, generation of reactive oxygen species, and immunocytochemistry, we demonstrated the NADPH oxidase activity is in neutrophils and not pancreatic acinar tissue. CONCLUSIONS:The results demonstrate a novel role for neutrophils infiltrating the pancreas in pathologic activation of digestive enzymes in acute pancreatitis and indicate that this effect is mediated by products of NADPH oxidase.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Gukovskaya AS,Vaquero E,Zaninovic V,Gorelick FS,Lusis AJ,Brennan ML,Holland S,Pandol SJdoi
10.1053/gast.2002.32409keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
974-84issue
4eissn
0016-5085issn
1528-0012pii
S0016508502027129journal_volume
122pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Proliferating cholangiocytes secrete and respond to neuroendocrine hormones, including secretin. We investigated whether secretin secreted by S cells and cholangiocytes stimulates biliary proliferation in mice. METHODS:Cholestasis was induced in secretin knockout (Sct(-/-)) and wild-type (control) mi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2014.02.030
更新日期:2014-06-01 00:00:00
abstract::Colon motility and myoelectric slow wave activity were compared in 10 patients with progressive systemic sclerosis and 18 controls. Recordings were made in the rectosigmoid and rectum, 25-30 cm and 10-15 cm from the anal margin, respectively, during two 4-min baselines and in response to stepwise distention of the col...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91567-9
更新日期:1989-02-01 00:00:00
abstract::The effects of intracerebroventricular and intravenous administration of corticotropin-releasing factor (CRF) on gastrointestinal motility, motilin-induced gastric motor response, and plasma motilin and somatostatin levels were investigated in fasted dogs chronically prepared with strain gauge transducers on the antru...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90690-6
更新日期:1986-10-01 00:00:00
abstract:BACKGROUND & AIMS:The anti-cancer effects of vegetables and fruit have been investigated extensively, but the association between vegetable and fruit consumption and risk of hepatocellular carcinoma (HCC) has not been quantified. We performed a meta-analysis of observational studies to clarify the association. METHODS...
journal_title:Gastroenterology
pub_type: 杂志文章,meta分析,评审
doi:10.1053/j.gastro.2014.08.005
更新日期:2014-11-01 00:00:00
abstract::An enteroscopic method that uses a pediatric colonoscope provides an alternative approach to the diagnostic and management problems arising in the setting of a Roux-en-Y limb hepaticojejunostomy or biliary diversion. Three patients with Roux-en-Y limb reconstructions who experienced recurrent cholangitis, recurrent pa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90305-8
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND & AIMS:Intestinal epithelial cells release antigen-presenting vesicles (exosomes) bearing major histocompatibility complex class II/peptide complexes stimulating specific immune responses in vivo. To characterize further the role of human epithelial exosomes in antigen presentation, their capacity to load an...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.02.043
更新日期:2007-05-01 00:00:00
abstract::The purpose of this study was to determine the utility of jejunal manometry in evaluating patients having partial small intestinal obstruction or the pseudoobstruction syndrome. We studied 10 healthy volunteers, 9 patients with documented mechanical obstruction, and 3 patients with chronic intestinal pseudoobstruction...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract:BACKGROUND & AIMS:Conventional transarterial chemoembolization (cTACE) is used to treat patients with hepatocellular carcinoma (HCC). Radioembolization is a minimally invasive procedure that involves implantation of radioactive micron-sized particles loaded with yttrium-90 (Y90) inside the blood vessels that supply a t...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2016.08.029
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND & AIMS:To elucidate the survival of the patients with unresectable hepatocellular carcinoma (HCC) who underwent transcatheter arterial lipiodol chemoembolization (TACE) and to analyze the factors affecting the survivals. METHODS:During the last 8 years, a nationwide prospective cohort study was performed in...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.05.021
更新日期:2006-08-01 00:00:00
abstract::The effect of magnesium on calcium- and pentagastrin-induced gastric acid secretion and on calcium-induced gastrin secretion were studied in healthy volunteers. Intravenous infusion of calcium gluconate increased serum gastrin concentration as well as gastric volume secretion, acidity, and acid output. Addition of mag...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-05-01 00:00:00
abstract::The aim of the present study was to characterize rat mucosal colonic cells harvested from the crypt continuum during differentiation and dimethylhydrazine-induced neoplasia. The collection of colonocytes was performed using a modified nonenzymatic isolation procedure based on Ca2+ chelation and gentle mechanical disso...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)80013-y
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND & AIMS:The carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a transmembrane glycoprotein with pleotropic functions, including clearance of hepatic insulin. We investigated the functions of the related protein CEACAM2, which has tissue-specific distribution (kidney, uterus, and crypt epi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.03.056
更新日期:2010-08-01 00:00:00
abstract::Several compounds, in particular bile acids, undergo enterohepatic circulation (EHC). Limited data are available on the pathophysiologic aspects of this circulation. In the present study we describe a surgical technique in rats that allows a long-term, reversible interruption of the EHC and monitoring of peripheral bl...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90499-8
更新日期:1985-02-01 00:00:00
abstract::This study is an examination of the association between Crohn's disease and psychiatric illness using careful gastrointestinal evaluation, a structured psychiatric interview of known reliability and validity, and explicit diagnostic criteria for psychiatric illness. A proband sample of 50 subjects with Crohn's disease...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract:BACKGROUND & AIMS:Gastric histology is not well studied in patients with Crohn's disease. The aim of this study was to analyze the histological appearance of gastric mucosa in patients with Crohn's disease. METHODS:In a prospective study, biopsy specimens taken from the antrum and body of 75 patients with known Crohn'...
journal_title:Gastroenterology
pub_type: 评论,杂志文章
doi:10.1053/gast.1997.v112.pm9041230
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND & AIMS:Although protein kinase C (PKC) alterations may play a role in the abnormal reactivity of cirrhotic rat aortas, its isoforms and cellular distribution are unknown. We therefore studied the protein expression and cellular distribution of PKC isoforms and their activation in cirrhotic rat aortas. METHO...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2000.8522
更新日期:2000-07-01 00:00:00
abstract::The leukocyte migration inhibition test has been used to investigate cellular immune responses to antigens in a protein fraction (BPF) of normal human gallbladder bile in patients with a variety of intra- and extrahepatic diseases. Inhibition of leukocyte migration in the presence of BPF was observed in 30 (81%) of 37...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Frequent occurrence of post-transfusion hepatitis continues despite screening for Australia antigen in blood before transfusion and elimination of commercial donor sources. The majority of these cases appears unrelated to hepatitis B virus. Preoperative, acute, and convalescent serra were screened for evidence of hepa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1975-12-01 00:00:00
abstract:BACKGROUND/AIMS:We have identified several clones specifically expressed during malignant cell proliferation by screening a complementary DNA library constructed from a human primary liver cancer with subtractive probes. One clone was identified as the glutamine synthetase (GS) transcript. Its expression is tightly reg...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90024-8
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND & AIMS:Seroclearance of hepatitis B surface antigen (HBsAg) is one of the most important clinical outcomes for chronic hepatitis B treatment trials. Few studies have explored the incidence and determinants of spontaneous seroclearance using a long-term follow-up study. This study aimed to examine the natural...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.04.048
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND & AIMS:Due to the phenotypic and molecular diversity of hepatocellular carcinomas (HCC), it is a challenge to determine a patient's prognosis. We aimed to identify new prognostic markers of patients with HCC treated by liver resection. METHODS:We collected 314 HCC samples from patients at Bordeaux (1998-200...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2013.03.051
更新日期:2013-07-01 00:00:00
abstract::Antibody prevalence and titer to rotavirus and Norwalk virus were studied in Crohn's disease patients and in age-, sex-, and time-matched controls. There were no significant antibody differences between the groups studied. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract:BACKGROUND & AIMS:In colorectal adenoma and carcinoma, glutathione S-transferase-pi (GSTP1-1) is highly expressed. K-ras mutation is also known to occur frequently in colorectal adenoma and carcinoma, as well as in the putative precursor of adenoma, aberrant crypt foci (ACF). Further, forced expression of v-H-ras in ra...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.27982
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND & AIMS:Corticofugal pathways to the human esophagus have been recently identified in humans using transcranial magnetic stimulation. The aim of this study was to determine the topographic representation of the esophagus on the cerebral cortex. METHODS:Focal magnetic stimulation of 1-cm areas was performed o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(96)70053-7
更新日期:1996-10-01 00:00:00
abstract::In sickle cell anemia the shortened survival of red blood cells presents the liver with an augmented load of bilirubin for hepatic clearance. To determine the effects of this excessive bilirubin load on the microsomal conjugating enzyme, hepatic bilirubin UDP-glucuronyltransferase, levels of this enzyme were measured ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::A case of enterogenous cysts of the duodenum in a 44-yr-old man complicated by duodenal obstruction and relapsing pancreatitis is presented. The cystic duodenal lesions were visualized by endoscopic retrograde cholangiopancreatography and for the first time by computed tomography and abdominal sonography. Thus, the di...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90157-x
更新日期:1985-03-01 00:00:00
abstract::This study presents normal values of bowel habits, colonic transit times of radioopaque markers, and anorectal pressure obtained from 114 normal subjects. Their stool frequency ranged from 3 to 11 per 7 days, whereas their diet contained an average of 14.4 g of crude fibers. Radioopaque markers progressed regularly al...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract:BACKGROUND & AIMS:Alcoholic patients with and without chronic liver disease have a high incidence of infection with hepatitis C virus (HCV). Long-term ethanol consumption in mice has been associated with a strikingly reduced CD8(+) cytotoxic T-lymphocyte (CTL) response to HCV nonstructural proteins following DNA-based ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.11.016
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND & AIMS:Advanced cirrhosis is associated with impaired leukocyte function, but the mechanism underlying this host defense alteration is unknown. The aim of this study was to investigate the lipoxygenase pathway of arachidonic acid metabolism and its influence in leukocyte trafficking in patients with cirrhosi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70376-2
更新日期:1998-07-01 00:00:00
abstract::A patient with gastric outlet syndrome (Bouveret's syndrome) caused by a large gallstone impacted in the duodenal bulb was successfully treated by extracorporeal shock-wave lithotripsy. Thus, open abdominal surgery could be avoided. For disintegration of the stone, three consecutive lithotripsy procedures were necessa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90086-3
更新日期:1989-08-01 00:00:00