Abstract:
BACKGROUND & AIMS:Proliferating cholangiocytes secrete and respond to neuroendocrine hormones, including secretin. We investigated whether secretin secreted by S cells and cholangiocytes stimulates biliary proliferation in mice. METHODS:Cholestasis was induced in secretin knockout (Sct(-/-)) and wild-type (control) mice by bile duct ligation (BDL). At days 3 and 7 after BDL, control and Sct(-/-) mice received tail-vein injections of morpholinos against microRNA 125b or let7a. One week later, liver tissues and cholangiocytes were collected. Immunohistochemical, immunoblot, luciferase reporter, and real-time polymerase chain reaction assays were performed. Intrahepatic bile duct mass (IBDM) and proliferation were measured. Secretin secretion was measured in conditioned media from cholangiocytes and S cells and in serum and bile. RESULTS:Secretin secretion was increased in supernatants from cholangiocytes and S cells and in serum and bile after BDL in control mice. BDL Sct(-/-) mice had lower IBDM, reduced proliferation, and reduced production of vascular endothelial growth factor (VEGF) A and nerve growth factor (NGF) compared with BDL control. BDL and control mice given morpholinos against microRNA 125b or let7a had increased IBDM. Livers of mice given morpholinos against microRNA 125b had increased expression of VEGFA, and those treated with morpholinos against microRNA let7a had increased expression of NGF. Secretin regulated VEGF and NGF expression that negatively correlated with microRNA 125b and let7a levels in liver tissue. CONCLUSIONS:After liver injury, secretin produced by cholangiocytes and S cells reduces microRNA 125b and let7a levels, resulting in up-regulation of VEGF and NGF. Modulation of cholangiocyte expression of secretin could be a therapeutic approach for biliary diseases.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Glaser S,Meng F,Han Y,Onori P,Chow BK,Francis H,Venter J,McDaniel K,Marzioni M,Invernizzi P,Ueno Y,Lai JM,Huang L,Standeford H,Alvaro D,Gaudio E,Franchitto A,Alpini Gdoi
10.1053/j.gastro.2014.02.030subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1795-808.e12issue
7eissn
0016-5085issn
1528-0012pii
S0016-5085(14)00241-8journal_volume
146pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Probiotics can reduce symptoms of irritable bowel syndrome (IBS), but little is known about their effects on psychiatric comorbidities. We performed a prospective study to evaluate the effects of Bifidobacterium longum NCC3001 (BL) on anxiety and depression in patients with IBS. METHODS:We performed ...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2017.05.003
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND & AIMS:Individuals with human immunodeficiency virus (HIV) infection frequently also are infected with hepatitis C virus (HCV) (co-infection), but little is known about its effects on the progression of HIV-associated disease. We aimed to determine the effects of co-infection on mortality from HIV and/or acq...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2012.12.026
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND & AIMS:Glutamine is an essential amino acid for rapidly dividing cells such as enterocytes. The progress of cancer is associated with a decrease of arterial and muscle glutamine concentrations. The aim of this study was to test whether increasing tumor loads affect gut intracellular glutamine handling, prote...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70226-9
更新日期:1997-01-01 00:00:00
abstract::The results of balloon dilatation of upper digestive tract stricture in 111 patients were evaluated. Eighty-eight patients had esophageal strictures and 23 had gastric or pyloric strictures. Thirty-six patients had strictures associated with previous operations. Twenty-two percent of the patients with esophageal stric...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90449-4
更新日期:1985-09-01 00:00:00
abstract::Primary sclerosing cholangitis is a chronic cholestatic liver disease that is commonly associated with chronic ulcerative colitis. We observed the development of varices in the abdominal wall surrounding the ileostomy stoma of patients with primary sclerosing cholangitis who underwent proctocolectomy and ileostomy for...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1016/0016-5085(86)90926-1
更新日期:1986-02-01 00:00:00
abstract:BACKGROUND & AIMS:Hepatitis G virus (HGV) has been recently identified as a new transfusion-transmissible agent. This study was performed to evaluate the role of HGV infection in patients with acute posttransfusion hepatitis in Taiwan. METHODS:Sera from 63 patients with acute posttransfusion hepatitis and 61 patients ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70138-0
更新日期:1997-04-01 00:00:00
abstract:BACKGROUND & AIMS:Recent studies indicate that kringle 1-5 has a potent and specific antiangiogenic activity. Here, we investigated the antitumor effect of kringle 1-5 gene transfer on hepatocellular carcinoma in mice. METHODS:The inhibitory effect of kringle 1-5 protein on proliferation of bovine capillary endothelia...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.02.020
更新日期:2006-04-01 00:00:00
abstract::The effect of somatostatin 28, a noval intestinal peptide containing somatostatin in its C-terminal portion, was tested on basal exocrine pancreatic secretion on conscious dogs and after stimulation by secretin (1 CU x kg-1 x hr-1), caerulein (37.5 ng x kg-1 x hr-1) or secretin (2 CU x kg-1 x hr-1) caerulein (37.5 ng ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract:BACKGROUND & AIMS:Patients with chronic hepatitis C and persistently normal alanine aminotransferase (ALT) levels have been routinely excluded from large randomized treatment trials; consequently, the efficacy and safety of antiviral therapy in this population are unknown. METHODS:Patients with at least 3 normal ALT v...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2004.09.050
更新日期:2004-12-01 00:00:00
abstract::Gastrinomas were localized by concurrent blood sampling in the hepatic vein and portal vein tributaries in 10 of 12 Zollinger-Ellison patients. In one patient, the portal vein could not be catheterized; in the other, a gastrin gradient could not be picked up in either of two examinations. Six of the patients were subs...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND & AIMS:Few data are available concerning the long-term prognosis of chronic liver disease associated with hepatitis C virus infection. This study examined the morbidity and survival of patients with compensated cirrhosis type C. METHODS:A cohort of 384 European cirrhotic patients was enrolled at seven terti...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v112.pm9024300
更新日期:1997-02-01 00:00:00
abstract::Several single-nucleotide polymorphisms (SNPs) have been associated with colorectal cancer (CRC) susceptibility. Most CRCs arise from adenomas, and SNPs therefore might affect predisposition to CRC by increasing adenoma risk. We found that 8 of 18 known CRC-associated SNPs (rs10936599, rs6983267, rs10795668, rs3802842...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.09.016
更新日期:2013-01-01 00:00:00
abstract::Six patients (4 women, 2 men) with malignant gastrinoma developed multiple bone metastases; osteolytic as well as osteoblastic lesions occurred. All lesions involved the central skeleton, most caused symptoms, and, in 2 cases, there was associated hypercalcemia with normal serum parathormone levels. Poor responses wer...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(86)80014-2
更新日期:1986-11-01 00:00:00
abstract::Aminopeptidase M (APM) was localized in the kidney and alimentary tract of guinea pigs and rats by indirect immunohistochemistry. APM was detected in the brush border of the epithelium of the proximal convoluted tubule of the kidney and of the small intestine, and it was localized to cells scattered throughout lymphoi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)90307-k
更新日期:1992-06-01 00:00:00
abstract:BACKGROUND & AIMS:Point mutations of the Ki-ras gene at codon 12 have been frequently identified in pure pancreatic juice of patients with pancreatic cancer in studies examining pancreatic cancer tissues. The aim of this study was to examine mutations of the Ki-ras codon 12 in the duodenal juice collected from patients...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v110.pm8536860
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Whether long-term octreotide treatment given to portal hypertensive rats could prevent or ameliorate peripheral vasodilatation and thereby modify sodium retention was investigated. METHODS:Starting at the time of partial portal vein ligation or sham operation, rats received a 4-day course of either octreoti...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(93)90429-g
更新日期:1993-02-01 00:00:00
abstract::Biofeedback has been advocated as first-line therapy for patients whose symptoms of mild to moderate fecal incontinence have not responded to simple dietary advice or medication. Three main modalities have been described: (1) use of an intra-anal electromyographic sensor, a probe to measure intra-anal pressure, or per...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2003.10.058
更新日期:2004-01-01 00:00:00
abstract::Four patients who underwent transplantation for hepatitis B virus-related liver disease developed rapidly progressive liver failure attributable to recurrent hepatitis B disease typified by hyperbilirubinemia and distinctive hepatocyte ballooning and progressive fibrosis consistent with recently reported fibrosing cho...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91521-5
更新日期:1992-10-01 00:00:00
abstract::Non-major histocompatibility complex-restricted cytotoxicity triggered by antibodies to the CD3 component of the human T-cell receptor complex is thought to be an indirect measure of in vivo primed cytotoxic T-cell activity. We have used this technique to examine the lytic activity of freshly isolated T cells from non...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90554-9
更新日期:1988-04-01 00:00:00
abstract::In the TMC435-C101 study, 6 patients infected with hepatitis C virus genotype 1 were treated with the protease inhibitor TMC435 (200 mg once daily) as monotherapy for 5 days. Approximately 1.5 years later, 5 of these patients were re-treated with TMC435 (200 mg once daily) plus pegylated interferon alfa-2a and ribavir...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1053/j.gastro.2012.07.117
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND & AIMS:Mutations in TP53, a tumor suppressor gene, are associated with prognosis of many cancers. However, the prognostic values of TP53 mutation sites are not known for patients with hepatocellular carcinoma (HCC) because of heterogeneity in their geographic and etiologic backgrounds. METHODS:TP53 mutation...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.11.034
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND/AIMS:Reflex relaxation is a mechanism whereby the colon can show short-term dilatation in the absence of mechanical obstruction. This study investigated the tonic response of the canine colon to ileal distention and its pharmacological control. METHODS:In four dogs, the tone of the proximal colon was record...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90692-0
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND/AIMS:Previous studies have shown that psychological factors play a role in symptom perception among patients with gastroesophageal reflux disease. This report describes the first controlled study showing the effects of relaxation training on symptom reports and esophageal acid exposure in patients with reflu...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(94)90061-2
更新日期:1994-07-01 00:00:00
abstract::The purposes of this study were to determine the association, in 10 pedigrees, between adenomatous polyposis coli, hereditary nonpolyposis colorectal cancer, and occult radiopaque jaw lesions, and to assess whether these radiodensities are predictors for adenomatous polyposis. In seven kindreds with adenomatous polypo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)90910-3
更新日期:1987-09-01 00:00:00
abstract::In vivo and in vitro ultrasonic characteristics of gallstones were compared with stone size and composition with the aim of understanding the basis for these features. In vivo and in vitro ultrasonic features of the same stone were identical. Acoustic shadowing was not due to stone type, radiodensity, or calcium conte...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::Most studies show that oral ingestion of iron does not cause a positive stool guaiac reaction. However, all in vitro studies show that iron does cause a positive guaiac reaction and some in vivo studies have shown a positive stool guaiac reaction in response to oral iron. This study examines this unresolved question. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90766-x
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND & AIMS:To define signaling events initiating healing after intestinal epithelial injury, activation of mitogen-activated protein kinase (MAPK) pathways was assessed after wounding using an in vitro model. METHODS:Proteins isolated from wounded monolayers of nontransformed intestinal epithelial cells (IEC-6)...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70583-9
更新日期:1998-04-01 00:00:00
abstract::Canine jejunal epithelial cells were isolated and maintained in short-term culture to study cholecystokinin (CCK) release. Sequential digestion of jejunal mucosa with collagenase and ethylenediaminetetraacetic acid was followed by counterflow elutriation to enrich CCK-containing cells. After 40 hours in culture on col...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91780-8
更新日期:1992-01-01 00:00:00
abstract::Crohn's disease and ulcerative colitis are heterogeneous inflammatory bowel diseases, and therapeutic requirements vary among patients. We have a limited capacity to predict disease progression for individual patients, therefore it is important that they are evaluated for the presence of active disease when symptoms a...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2016.10.005
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND & AIMS:Reactive oxidative species (ROS) are believed to be involved in the progression of nonalcoholic steatohepatitis (NASH). However, little is known about the sources of ROS in hepatocytes or their role in disease progression. We studied the effects of nicotinamide adenine dinucleotide phosphate reduced o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.04.009
更新日期:2015-08-01 00:00:00