Evidence for a role of nitric oxide in the mediation of antiproliferative UVA effects in keratinocytes.


:Using cultured human keratinocytes, the present study investigates the role of nitric oxide (NO) in the mediation of the antiproliferative effects of ultraviolet light A (UVA). UVA treatment of cells (3-21 J cm (-2)) caused a time- and dose-dependent increase in nitrite formation in a micromolar range. This effect was accompanied by a decrease in DNA synthesis by 53.5%. Moreover, UVA treatment slightly reduced cell viability by 23.8%. Preincubation of keratinocytes with the NO scavenger 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO, 10-100 microM) or the NO synthase inhibitor N(G)-monomethyl-l-arginine (l-NMMA, 30-300 microM) significantly diminished the UVA-induced increase in nitrite. PTIO as well as l-NMMA partially protected keratinocytes from UVA-induced antiproliferative effects and increased DNA synthesis by 67 or 49% of the control. The co-application of UVA irradiation (10 J cm (-2)) and the essential cofactor of NO synthases tetrahydrobiopetrin (BH4, 500 microM) led to an overadditive increase in the release of nitrite as well as to a decrease in DNA synthesis. These results imply that NO is involved in the antiproliferative UVA effects in keratinocytes.


Pharmacol Res


Pharmacological research


Podhaisky HP,Klapperstück T,Riemschneider S,Wohlrab W





Has Abstract


2002-03-01 00:00:00














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    abstract::The effect of age and sex on the predictive value of colorectal cancer (CRC) patients treated with immune checkpoint inhibitors (ICIs) has been controversial, and the effect of specific gene mutations on the predictive value of CRC patients treated with ICIs remains to be explored. Our study analyzed the influence of ...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Lin A,Zhang H,Hu X,Chen X,Wu G,Luo P,Zhang J

    更新日期:2020-09-01 00:00:00

  • Tumor-derived extracellular vesicles: Regulators of tumor microenvironment and the enlightenment in tumor therapy.

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    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Chen J,Fei X,Wang J,Cai Z

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  • Paradoxical sleep deprivation in rats causes a selective reduction in the expression of type-2 metabotropic glutamate receptors in the hippocampus.

    abstract::Paradoxical sleep deprivation in rats is considered as an experimental animal model of mania endowed with face, construct, and pharmacological validity. We induced paradoxical sleep deprivation by placing rats onto a small platform surrounded by water. This procedure caused the animal to fall in the water at the onset...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Panaccione I,Iacovelli L,di Nuzzo L,Nardecchia F,Mauro G,Janiri D,De Blasi A,Sani G,Nicoletti F,Orlando R

    更新日期:2017-03-01 00:00:00

  • N-acetyl penicillamine a protector of Plasmodium berghei induced stress organ injury in mice.

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    pub_type: 杂志文章


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    更新日期:1997-10-01 00:00:00

  • MicroRNAs in chronic airway diseases: Clinical correlation and translational applications.

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    journal_title:Pharmacological research

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  • A TCM formula comprising Sophorae Flos and Lonicerae Japonicae Flos alters compositions of immune cells and molecules of the STAT3 pathway in melanoma microenvironment.

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    更新日期:2019-04-01 00:00:00

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    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Rózsai B,Lázár Z,Benkó R,Barthó L

    更新日期:2001-01-01 00:00:00

  • The effect of manganese chloride on gentamicin-induced nephrotoxicity in rats.

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    authors: Razzolini R,Boffa GM,Calvanese A,Isabella G,Ramondo A,Chioin R

    更新日期:1991-10-01 00:00:00

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    更新日期:2009-06-01 00:00:00

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    authors: Ozmen I,Küfrevioglu OI

    更新日期:2004-11-01 00:00:00

  • Green tea polyphenols produce antidepressant-like effects in adult mice.

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    pub_type: 临床试验,杂志文章


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    pub_type: 杂志文章,评审


    authors: Sánchez-García A,Simental-Mendía M,Millán-Alanís JM,Simental-Mendía LE

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  • Protection from apoptotic cell death by cilostazol, phosphodiesterase type III inhibitor, via cAMP-dependent protein kinase activation.

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    更新日期:2006-10-01 00:00:00

  • Prefrontal-accumbens opioid plasticity: Implications for relapse and dependence.

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    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


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    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Bátkai S,Pacher P

    更新日期:2009-08-01 00:00:00

  • Janus kinase (JAK) inhibitors in the treatment of inflammatory and neoplastic diseases.

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    更新日期:2016-09-01 00:00:00

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    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Gambardella A,Labate A,Cifelli P,Ruffolo G,Mumoli L,Aronica E,Palma E

    更新日期:2016-11-01 00:00:00

  • Interplay between nitric oxide and CGRP by capsaicin in isolated guinea-pig heart.

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    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Oroszi G,Szilvassy Z,Nemeth J,Tosaki A,Szolcsanyi J

    更新日期:1999-08-01 00:00:00

  • Gene silencing of receptor-interacting protein 2 protects against cigarette smoke-induced acute lung injury.

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    pub_type: 杂志文章


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    更新日期:2019-01-01 00:00:00

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    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Giannessi D,Caselli C,Vitale RL,Crucean A,Murzi B,Del Ry S,Vanini V,Biagini A

    更新日期:2003-11-01 00:00:00

  • The soy phytoestrogens genistein and daidzein as neuroprotective agents against anoxia-glucopenia and reperfusion damage in rat urinary bladder.

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    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Valeri A,Fiorenzani P,Rossi R,Aloisi AM,Valoti M,Pessina F

    更新日期:2012-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Gómez-Escribano AP,Bono-Yagüe J,García-Gimeno MA,Sequedo MD,Hervás D,Fornés-Ferrer V,Torres-Sánchez SC,Millán JM,Sanz P,Vázquez-Manrique RP

    更新日期:2020-11-01 00:00:00

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    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Akici A,Karaalp A,Skender E,El-Fakahany EE,Oktay S

    更新日期:1999-11-01 00:00:00