Abstract：
:Using cultured human keratinocytes, the present study investigates the role of nitric oxide (NO) in the mediation of the antiproliferative effects of ultraviolet light A (UVA). UVA treatment of cells (3-21 J cm (-2)) caused a time- and dose-dependent increase in nitrite formation in a micromolar range. This effect was accompanied by a decrease in DNA synthesis by 53.5%. Moreover, UVA treatment slightly reduced cell viability by 23.8%. Preincubation of keratinocytes with the NO scavenger 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO, 10-100 microM) or the NO synthase inhibitor N(G)-monomethyl-l-arginine (l-NMMA, 30-300 microM) significantly diminished the UVA-induced increase in nitrite. PTIO as well as l-NMMA partially protected keratinocytes from UVA-induced antiproliferative effects and increased DNA synthesis by 67 or 49% of the control. The co-application of UVA irradiation (10 J cm (-2)) and the essential cofactor of NO synthases tetrahydrobiopetrin (BH4, 500 microM) led to an overadditive increase in the release of nitrite as well as to a decrease in DNA synthesis. These results imply that NO is involved in the antiproliferative UVA effects in keratinocytes.
journal_name
Pharmacol Resjournal_title
Pharmacological researchauthors
Podhaisky HP,Klapperstück T,Riemschneider S,Wohlrab Wdoi
10.1006/phrs.2001.0939keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
229-33issue
3eissn
1043-6618issn
1096-1186pii
S1043661801909399journal_volume
45pub_type
杂志文章abstract：:This study aimed to elucidate whether the effect of cilostazol to suppress apoptotic cell death is directly coupled to cAMP-dependent protein kinase activation in human umbilical vein endothelial cells (HUVECs). After exposure of HUVECs to LPS (1 microgml(-1)) for 18 h, the endothelial cells irregularly aggregated wit...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2006.05.006
更新日期：2006-10-01 00:00:00
abstract：:Gossypol, a cottonseed extract, has been shown to have antiproliferative activity in a variety of cancer cell lines. The objective of this study was to determine the inhibitory effects of gossypol on cell proliferation. Five human carcinoma cell lines were evaluated including endometrial (RL95-2), ovarian (SKOV-3), me...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/s104366180200230x
更新日期：2002-12-01 00:00:00
abstract：:Nebivolol is a new selective beta 1-adrenergic blocking agent, that possesses a peculiar pharmacodynamic profile and an original chemical structure, by which it differs from traditional beta 1-blockers. Nebivolol is a racemic mixture of two enantiomers in equal ratios. It is endowed with a highly selective beta 1-bloc...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1006/phrs.1998.0387
更新日期：1998-12-01 00:00:00
abstract：:In the isolated myometrium obtained from pregnant women, diphenylhydantoin inhibited both spontaneous and K(+)-evoked contractions within the same range of potency. Nicardipine was less effective in inhibiting spontaneous than K(+)-induced contractions, whereas trifluoperazine was only effective against spontaneous co...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/s1043-6618(05)80051-9
更新日期：1990-09-01 00:00:00
abstract：:The most frequent cause of mortality and morbidity in industrialized countries is coronary artery disease (CAD), which in Europe alone is responsible for around two million deaths per year. In 2001 it accounted for about 260,000 hospital discharges in Italy. The costs of CAD treatment in Italy--which were borne by the...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2006.04.002
更新日期：2006-08-01 00:00:00
abstract：:The effect of age and sex on the predictive value of colorectal cancer (CRC) patients treated with immune checkpoint inhibitors (ICIs) has been controversial, and the effect of specific gene mutations on the predictive value of CRC patients treated with ICIs remains to be explored. Our study analyzed the influence of ...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2020.105028
更新日期：2020-09-01 00:00:00
abstract：:Parkinson's disease is a progressive age-related neurodegenerative disease with invariant loss of substantia nigra dopamine neurons and striatal projections. This disorder is well known for the associated motoric symptoms including resting tremor and the inability to initiate movement. However, it is now apparent that...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2008.09.006
更新日期：2008-11-01 00:00:00
abstract：:Alcohol abuse and addiction are serious global health problems. Tackling these disorders requires an understanding of how ethanol produces its effects. Early cell culture studies implicated the protein kinase C (PKC) family of serine-threonine kinases in mediating both acute and chronic responses to ethanol exposure. ...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2007.04.008
更新日期：2007-06-01 00:00:00
abstract：:Folic acid-conjugated albumin nanospheres (FA-AN) have been developed to provide an actively targetable drug delivery system for improved drug targeting of cancer cells with reduced side effects. The nanospheres were prepared by conjugating folic acid onto the surface of albumin nanospheres using 1-ethyl-3-(3-dimethyl...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2010.10.012
更新日期：2011-01-01 00:00:00
abstract：:This study was performed in order to investigate the cholinomimetic response of seminal vesicles isolated from rats treated with hydrocortisone acetate during perinatal life. At the adult phase, the body weight and the wet weight of the seminal vesicle of these animals were unchanged. However, these male rats exhibite...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Quiescent hepatic stellate cell (HSC) activation and subsequent conversion into myofibroblasts is the central event in hepatic fibrosis pathogenesis. Epithelial-mesenchymal transition (EMT), another vital participant in liver fibrosis, has the potential to initiate HSC activation, which promotes abundant myofibroblast...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2017.10.013
更新日期：2018-02-01 00:00:00
abstract：:In the present study we evaluated the protective activity of pyridoxol L,2-pyrrolidon-5 carboxylate (metadoxine) against CCl4 intoxication in rats, especially in relation to liver fibrosis. After 6 consecutive weeks of CCl4 treatment, the animals developed liver fibrosis and inflammation as revealed by histological an...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/s1043-6618(05)80067-2
更新日期：1992-01-01 00:00:00
abstract：:Monoclonal antibodies (mAb) have revolutionised the way in which we treat disease. From cancer to autoimmunity, antibody therapy has been responsible for some of the most impressive clinical responses observed in the last 2 decades. A key component of this success has been their generally low levels of toxicity, and u...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2015.07.003
更新日期：2015-09-01 00:00:00
abstract：:The Janus kinase (JAK) family of non-receptor protein-tyrosine kinases consists of JAK1, JAK2, JAK3, and TYK2 (tyrosine kinase-2). Each of these proteins contains a JAK homology pseudokinase (JH2) domain that regulates the adjacent protein kinase domain (JH1). JAK1/2 and TYK2 are ubiquitously expressed whereas JAK3 is...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2016.07.038
更新日期：2016-09-01 00:00:00
abstract：:Metastatic and primary bone cancers are usually accompanied by severe pain that is difficult to manage. In light of the adverse side effects of opioids, manipulation of the endocannabinoid system may provide an effective alternative for the treatment of cancer pain. The present study determined that a local, periphera...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2011.03.007
更新日期：2011-07-01 00:00:00
abstract：:Isoxanthohumol (IXN), a prenylated flavonoid from hops, exhibits diverse biological activities, e.g. antitumor, antiinflammatory, antioxidant and antiangiogenic. In this study, the effect of IXN is evaluated on two melanoma cell lines with dissimilar molecular background, B16 and A375. The treatment of both cell lines...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2016.01.011
更新日期：2016-03-01 00:00:00
abstract：:Combination antiretroviral therapy reduces morbidity and mortality in HIV infected patients. However, the cure of HIV infection is hindered by the persistence of the latent HIV reservoir. Latency reversing agents (LRAs) are developed to target the HIV latently infected cells for HIV reactivation. In addition to revers...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2018.10.023
更新日期：2019-01-01 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a debilitating condition where excess collagen deposition occurs in the extracellular matrix. At first sight, it is expected that the level of different kinds of matrix metalloproteinases might be downregulated in IPF as it is a matrix degrading collagenase. However, the role of ...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2019.104591
更新日期：2020-02-01 00:00:00
abstract：:Ranolazine has characteristic properties of a selective inhibitor of the inward sodium current. It is primarily indicated as an anti-anginal agent in patients with coronary artery disease and chronic stable angina. Recently, ranolazine has been noted to possibly impart beneficial effects in various other cardiac condi...
journal_title：Pharmacological research
pub_type： 杂志文章,评审
doi：10.1016/j.phrs.2015.10.018
更新日期：2016-01-01 00:00:00
abstract：:The purpose of the present work was to study the change in morphine metabolic ratio in obese subjects before and after Roux-en-Y Gastric Bypass (RYGB) and to identify clinical and/or biological factors associated with this change. The pharmacokinetics (PK) of oral morphine (30mg), morphine-3-glucuronide (M3G) and morp...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2016.08.031
更新日期：2017-04-01 00:00:00
abstract：:The potential drug-to-drug interaction between darunavir and raltegravir in the setting of HIV infection is a highly debated issue still unresolved. In the present study we have evaluated the pharmacokinetics of darunavir and ritonavir in 53 HIV-1 infected patients with or without concomitant raltegravir administratio...
journal_title：Pharmacological research
pub_type： 临床试验,杂志文章
doi：10.1016/j.phrs.2011.09.006
更新日期：2012-02-01 00:00:00
abstract：:It has been suggested that hypertension results from a loss of immunological tolerance and the resulting autoimmunity may be an important underlying factor of its pathogenesis. This stems from the observations that many of the features involved in autoimmunity are also implicated in hypertension. Furthermore, the unde...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2016.09.008
更新日期：2016-11-01 00:00:00
abstract：:The present study was designed to investigate the ameliorative potential of rosiglitazone, a peroxisome proliferator-activated receptor gamma (PPAR gamma) agonist, in tibial and sural nerve transection-induced neuropathic pain in rats. The pinprick, cold immersion, hot plate and hot immersion tests were performed to a...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:The synergistic protective effect of nicorandil (KATP channel opener) and amlodipine (calcium channel blocker) on mitochondrial respiration and mitochondrial lipid contents were examined on isoproterenol-induced myocardial infarction in rats. The rats given isoproterenol (150 mg kg(-1) daily, i.p.) for 2 days showed s...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/s1043-6618(03)00223-8
更新日期：2003-12-01 00:00:00
abstract：:Flibanserin has been reported to be an agonist at 5-HT1A-receptors and an antagonist at 5-HT2A receptors, with higher affinity for 5-HT1A receptors. Despite the fact that less receptor occupation is required by full agonists than by antagonists to exert their effects, flibanserin was shown to exert 5-HT2A antagonism a...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1006/phrs.2000.0762
更新日期：2001-02-01 00:00:00
abstract：:Breast cancer, the most prevalent cancer in women, remains the second in the list of cancer mortality, the majority of these fatalities resulted from estrogen receptor alpha (ERα) positive disease. ERα is well known for its function on breast cancer initiation and development and has become the most successful biomark...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2019.104294
更新日期：2019-08-01 00:00:00
abstract：:Ocotea quixos essential oil was shown to possess significant inhibitory activity of platelet aggregation and clot retraction in rodent plasma. This study is aimed at fully characterizing the antiplatelet activity of the whole essential oil and its main components trans-cinnamaldehyde and methyl cinnamate also in human...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2006.09.009
更新日期：2007-01-01 00:00:00
abstract：:The effects of betel leaf extract (0.10, 0.40, 0.80 and 2.0 g kg-1 day-1 for 15 days) on the alterations in thyroid hormone concentrations. lipid peroxidation (LPO) and on the activities of superoxide dismutase (SOD) and catalase (CAT) were investigated in male Swiss mice. Administration of betel leaf extract exhibite...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1006/phrs.1998.0398
更新日期：1998-12-01 00:00:00
abstract：:The aim of this study was to verify the role of Vitamins C and E on the cognitive function of young and aged mice. First and second groups of young animals (aged 3 months) received either Vitamin E (250mg/kg per day) or Vitamin C (300mg/kg per day) for 60 days. Third group was treated with the combination of Vitamin E...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2003.10.004
更新日期：2004-03-01 00:00:00
abstract：:As the capacity of the endogenous antioxidative system is limited, pharmacological treatment with antioxidants may help to protect neuronal tissue against increased amount of reactive oxygen species produced during oxidative stress. We attempted to improve resistance of rat hippocampal slices exposed to ischaemia in v...
journal_title：Pharmacological research
pub_type： 杂志文章
doi：10.1016/j.phrs.2005.08.004
更新日期：2006-01-01 00:00:00
