Abstract:
OBJECTIVE:The purpose of this study was to collect data about the incidence of high-risk HPV (16, 18, 33) types in in situ cervical cancers, and to evaluate the reliability of the morphological signs of HPV infection by comparing the presence of these signs to the PCR-proven HPV virus infection. METHODS:Fifty patients who underwent conisation at the Department of Obstetrics and Gynecology of Semmelweis University, Budapest, Hungary because of in situ cervical cancer were examined retrospectively for the presence of HPV infection by the PCR technique. The direct and indirect morphological signs of HPV infection identified in the histological and cytological samples were compared to the actual results of virus DNA amplification by PCR in the identical histological sections. The evaluation of the cytological smears and the histological sections was accomplished independently by two different pathologists. RESULTS:E6 open reading frame of HPV 16, 18 or 33 was detected by PCR in 56% (28 cases) of the histological sections of the 50 examined patients with in situ cancer. In 92% (26 patients) of the 28 HPV positive patients one HPV type was detected, while in one of the remaining two cases two HPV types (16/33), or all three types could be detected. The direct morphological signs for HPV infection proved to be 75% sensitive and 50% specific when compared to the results of PCR. Their predictive value for HPV infection was 65%. For the indirect HPV signs the sensitivity was 64% and specificity 31%. The predictive value, prognosticating the presence of HPV 16, 18, 33 infection was 54% in the same sections. Using significance analysis no significant relationship (p = 0.7728) could be detected between the positivity of indirect signs and the presence of HPV 16, 18, 33 infection, while in case of direct signs the relationship was almost significant (p = 0.0675). The joint testing of the direct and indirect signs did not improve the results (p = 0.1338). During the review of the cytological smears the specificity of the cytology in predicting true HPV infections was found to be 68% and sensitivity was 20%. The predictive value was only 50%. A significance analysis was not accomplished by this diagnostic method because of the missing data (see text). CONCLUSION:The method of Nawa et al. seems to be a reliable approach for the detection of HPV DNA in paraffin-embedded material. The three main types of HPV (16, 18, 33) are probably represented in lower percentages in CIN III in Hungary, but a larger survey is needed to obtain reliable data. The direct and indirect morphological signs of HPV infection failed to show a significant relationship with the PCR proven presence of HPV 16, 18, 33.
journal_name
Eur J Gynaecol Oncoljournal_title
European journal of gynaecological oncologyauthors
Pete I,Szirmai K,Csapó Z,Szánthó A,Füle T,Gallai M,Kovalszky Ikeywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
74-8issue
1eissn
0392-2936issn
2709-0086journal_volume
23pub_type
杂志文章abstract::The usefulness of urine cyclic guanosine 3'-5'monophosphate (GMP (cGMP) as a cancer marker in ovarian cancer has been studied in two independent centres using specimens from 81 women. Serial specimens from 60 patients were monitored up to 2 years after the start of chemotherapy. Urine cGMP levels were significantly hi...
journal_title:European journal of gynaecological oncology
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVES:To estimate the prognostic value of pelvic-node removal on survival of patients affected by endometrial carcinoma at presurgical FIGO Stage I. METHODS:A retrospective analysis was performed on a total of 111 patients recruited from 1990 to 1996 at the S. Carlo di Nancy Hospital. Thirty-nine (35%) of them un...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::We studied a group of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) wer...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:INTRODUCTION:Malignant mixed mullerian tumor (MMMT) of the ovary is an extremely rare gynaecologic neoplasm that represents 1% of the malignances of this organ. Stage I disease is rare because it is asymptomatic in early stage. We describe four cases. CASE REPORTS:In the Department of Obstetrics and Gynecology of the ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Primary squamous cell carcinoma of the endometrium (PSCCE) is a rare disease of unknown etiology. Diagnosis is based on the identification of squamous cell carcinoma in the endometrium with no coexisting analogous cervical component or endometrial adenocarcinoma. There must also be no connection between the endometria...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Changes in CA 125 antigen concentration and serum half-life were determined in 63 women with ovarian carcinoma during chemotherapy following various types of surgery. Concentration of CA 125 in serum correlated with the degree of clinical advancement of the tumor, 20.00 and 688.84 U per ml at stages I, II, and IV, res...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Optimal cytoreduction is a major prognostic factor in ovarian cancer; several clinical, radiological and biochemical predictors have been studied. Tumour M2-PK (TU M2-PK) is over-expressed in tumour cells and can be detected in plasma samples but its role in ovarian cancer has not yet been evaluated. OBJECT...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:AIM OF THE STUDY:The analysis of acute and late toxicity of concurrent chemoradiotherapy (CCRT) for locally advanced cervical cancer (LACC) based on review of 120 patients treated in Centre of Oncology in Krakow between 2001 and 2007. MATERIALS AND METHODS:Medium age of the patients was 52 years (43-66). Overall, 12 p...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Four patients with hypersensitivity reaction to carboplatin of variable severity, after previous uneventful cisplatin and carboplatin treatment, are described. Skin testing performed in two of the patients suggests a cross-reaction with cisplatin but was negative with carboplatin in one of them. The mechanism of hyper...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Sixty four patients with epithelial ovarian cancer underwent second-look laparotomy. Influencing factors on the outcome of the second-look laparotomy were analysed. Both grade and stage appeared to have an effect on the outcome of the procedure. However, this effect appeared to be dependent on the amount of residual d...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Metastasis to the cervix and parametrium from breast carcinoma is a rare occurrence. CASE REPORT:A 74-year-old woman presented to the urologists with loin pain and microscopic haematuria. Investigations revealed a right ureteric obstruction which was diagnosed to be secondary to parametrial metastasis from ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract:PURPOSE OF INVESTIGATION:Infertility represents one of the main sequelae of cytotoxic therapy given for various malignant diseases. Because dividing cells are more sensitive to cytotoxic effects than are cells at rest, it has been hypothesized that inhibition of the pituitary-gonadal axis may facilitate the preservatio...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:AIM:Examination of the potential role of leptin in the development of uterine myomas. Expression of the leptin gene and leptin receptor gene was tested in the myometrium of healthy women, and in myomas and the surrounding myometrium of women with benign tumors. METHODS:Using RT-PCR, expression of the leptin gene and l...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:PURPOSE OF INVESTIGATION:To identify anaesthesia related risk factors associated with positive second-look laparotomy (SLL) findings in patients with epithelial ovarian carcinoma who had previous optimal cytoreduction surgery under general anaesthesia. METHODS:A retrospective review of the anaesthesia and medical reco...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::A case of müllerian adenosarcoma with sarcomatous overgrowth in a postmenopausal 66-year-old female patient after adjuvant tamoxifen treatment for breast carcinoma is described. The patient was asymptomatic and the neoplasm was detected by pelvic sonography. The diagnosis was based on the histological findings after c...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:To evaluate sensitivity and specificity of pre-operative and frozen section pathologic evaluation (FSA) in predicting high-risk (HR) histology endometrial cancer. MATERIALS AND METHODS:A retrospective analysis was performed on all patients diagnosed with endometrial cancer at a single institution. Medical reco...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::For 50 years, the three- and five-year survival rates of under 40% for invasive ovarian cancer in the USA have not significantly changed. Identifying those women who have a greater probability of developing the disease should contribute to improving survival. Our 3-year case-control study of 298 women from the metropo...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:PURPOSE OF INVESTIGATION:To identify genetic imbalance related to chemoresistance in epithelial ovarian cancer. METHODS:We screened abnormal chromosomal regions in cisplatin-resistant and cisplatin-sensitive human ovarian cancer cell lines employing comparative genomic hybridization (CGH). We also documented non-rando...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The Authors studied the presence of some markers in different gynaecological tumours, by radioimmunoassay. Beta-HCG, 1-alfa-FP, CA 125, GICA and TPA were assayed in 76 patients. Eight of the patients were affected by CIN III, 38 presented a cervical carcinoma (10 at stage I, 14 at stage II, 10 at stage III, 4 at stage...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:Ovarian clear cell adenocarcinoma (OCCA) has been reported to display different characteristics from other histological types of epithelial ovarian cancer, and especially differs from serous adenocarcinoma. We investigated plasminogen activator inhibitor-1 (PAI-1) expression in patients with OCCA and attempted ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To report the first case of a uterine gestational choriocarcinoma coexisting with an endometrial carcinoma (EC) and to discuss its possible pathogenesis. MATERIALS AND METHODS:All tissues were examined histologically and monoclonal antibodies were used to evaluate the expression of HCG, HPL, P53, PTEN, and E...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the clinical value of ultrasound-guided resection of non-palpable breast masses. MATERIALS AND METHODS:One hundred thirty-seven cases of non palpable breast mass patients who underwent surgical treatment from June 2007 to June 2012 were enrolled in this study. All patien...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::The efficacy of a new device, "the Endopap" endometrial cell sampler in obtaining specimens for cytological examination to detect precursors of malignancy of the endometrium was assessed in 140 asymptomatic and sympomatic women. The cytological results as compared with hystological diagnosis on curettage specimens by ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::1. The outcome of a course of radiotherapy is very dependent on the dose per fraction. The smaller the dose per fraction, as a general rule, the better the sparing of the late reacting normal tissues. 2. Overall treatment time is important, especially for tumours with a rapid doubling time. In such a case, the ideal o...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract:OBJECTIVE:We aimed to investigate the utility and efficacy of the Keyes skin biopsy instrument for cervical biopsy procedures. MATERIAL AND METHODS:A prospective clinical trial was conducted on 50 women with cervical lesions. Colposcopy-guided cervical biopsies were collected using a Keyes biopsy punch and a Kevorkian...
journal_title:European journal of gynaecological oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The aim of this study is to draw attention to the possibility of the occurrence of a metastatic spread at the laparoscopic entrance site in patients suffering from a carcinoma of the ovary. This event has rarely been mentioned in medical literature before. A case is presented here in which a localized tumoral lesion a...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Uterine tumors resembling ovarian sex cord tumors (UTROSCTs) are very rare uterine neoplasms. They mostly behave in a benign fashion but cases were reported with extra-uterine spread. There are less than 50 cases reported in the literature so far according to the present authors' knowledge. We report a case of 45-year...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::In an aim to evaluate the diagnostic efficacy of preoperative abdominal-pelvic CT for the prediction of suboptimal cytoreduction of epithelial ovarian carcinoma (EOC) at primary surgery, CT scans of 48 patients who underwent primary surgery for EOC were retrospectively analyzed. The presence of at least one of the fol...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::According to recent epidemiological studies on ovarian cysts during pregnancy one out of 600 are, in most cases, benign neoformations. The most frequent histological type reported is mature cystic teratoma (50% of the cases), followed by functional cysts (13%), benign cystadenomas (20%) and ovarian cancer (0.6%). Most...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVES:The prognostic impact of risk factors for ovarian cancer development is sparsely explored, but previous sterilisation has been shown to have a negative impact on survival. METHODS:Ovarian cancer cases were from the Danish MALOVA study. Information on previous pelvic surgery as well as reproductive variables...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00