Abstract:
AIM:To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis, depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma. METHODS:The expression of p16 protein was examined by streptavidin-peroxidase conjugated method (S-P);the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma. RESULTS:Expression of p16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of p16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P < 0.05). The positive rate of p16 protein expression in mucoid carcinoma 10.00% (1/10) was significantly lower than that in poorly differentiated carcinoma 51.22% (21/41), undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/16) (P < 0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P < 0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas. CONCLUSIONS:The expression loss of p16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
He XS,Su Q,Chen ZC,He XT,Long ZF,Ling H,Zhang LRdoi
10.3748/wjg.v7.i4.515keywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
515-21issue
4eissn
1007-9327issn
2219-2840journal_volume
7pub_type
杂志文章abstract:AIM:To evaluate different methods in differentiating idiopathic neonatal hepatitis from biliary atresia. METHODS:Sixty-five infants with cholestatic jaundice and final diagnosis of idiopathic neonatal hepatitis and biliary atresia were studied prospectively from September 2003 to March 2006. A thorough history and phy...
journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:10.3748/wjg.v12.i36.5893
更新日期:2006-09-28 00:00:00
abstract:AIM:To assess the risk factors and the efficacy of medications of patients with gastric and duodenal ulcers among Chinese patients in Taiwan. METHODS:Patients with peptic ulcers, diagnosed by upper endoscopy, were retrospectively collected between January 2008 and December 2008. The differences were compared. RESULTS...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i16.2017
更新日期:2010-04-28 00:00:00
abstract:AIM:To investigate the differences in injury patterns caused by arterial, venous or arteriovenous mesenteric occlusion. METHODS:Male Wistar rats were separated equally into four groups. Occlusion was performed by clamping the superior mesenteric artery (A), the mesenteric vein (V) or both (AV) for 30 min, followed by ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.3901
更新日期:2009-08-21 00:00:00
abstract:AIM:To investigate the effects of restraint stress on chronic colitis in interleukin (IL)-10 deficient (IL-10(-/-)) mice. METHODS:The first experiment compared the effect of restraint stress on the development of intestinal inflammation in wild-type and IL-10(-/-) mice. Both wild-type and IL-10(-/-) mice were physical...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i28.8580
更新日期:2015-07-28 00:00:00
abstract:AIM:To evaluate the rate of adverse events (AEs) during consecutive gastric and duodenal polypectomies in several Spanish centers. METHODS:Polypectomies of protruded gastric or duodenal polyps ≥ 5 mm using hot snare were prospectively included. Prophylactic measures of hemorrhage were allowed in predefined cases. AEs ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.3748/wjg.v23.i47.8405
更新日期:2017-12-21 00:00:00
abstract:BACKGROUND:Autophagy is an evolutionarily conserved biological process in eukaryotic cells that involves lysosomal-mediated degradation and recycling of related cellular components. Recent studies have shown that autophagy plays an important role in the pathogenesis of Crohn's disease (CD). Herbal cake-partitioned moxi...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i39.5997
更新日期:2020-10-21 00:00:00
abstract::A wide range of local thermal ablative therapies have been developed in the treatment of non resectable hepatocellular carcinoma (HCC) in the last decade. Laser ablation (LA) and radiofrequency ablation (RFA) are the two most widely used of these. This article provides an up to date overview of the role of laser ablat...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.14.7170
更新日期:2008-12-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i13.1975
更新日期:2004-07-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v19.i43.7603
更新日期:2013-11-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i25.5769
更新日期:2016-07-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i7.2373
更新日期:2016-02-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i4.516
更新日期:2013-01-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i44.8056
更新日期:2013-11-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i36.13153
更新日期:2014-09-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i8.1171
更新日期:2004-04-15 00:00:00
abstract::Most medical specialties including the field of gastroenterology are mainly aimed at treating diseases rather than preventing them. Genomic medicine studies the health/disease process based on the interaction of the human genes with the environment. The gastrointestinal (GI) system is an ideal model to analyze the int...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v23.i17.3030
更新日期:2017-05-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i39.7119
更新日期:2017-10-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i27.8751
更新日期:2014-07-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i34.10045
更新日期:2015-09-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 评论,信件
doi:10.3748/wjg.v17.i29.3465
更新日期:2011-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.6083
更新日期:2008-10-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v22.i9.2711
更新日期:2016-03-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i46.7549
更新日期:2006-12-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.4775
更新日期:2009-10-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i21.3217
更新日期:2005-06-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i47.5351
更新日期:2018-12-21 00:00:00
abstract::Percutaneous ablation using thermal or chemical methods has been widely used in the treatment of hepatocellular carcinoma (HCC). Nowadays, contrast-enhanced imaging modalities such as computed tomography (CT), magnetic resonance imaging (MRI), and contrast-enhanced ultrasound (CEUS) are widely used to evaluate local t...
journal_title:World journal of gastroenterology
pub_type: 评论,杂志文章
doi:10.3748/wjg.v19.i6.797
更新日期:2013-02-14 00:00:00
abstract:AIM:To clarify the previous discrepant conclusions, we performed a meta-analysis to evaluate the prognostic value of red cell distribution width (RDW) in esophageal cancer (EC). METHODS:We searched the PubMed, EMBASE, Web of Science and Cochrane Library databases to identify clinical studies, followed by using STATA v...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析,评审
doi:10.3748/wjg.v24.i19.2120
更新日期:2018-05-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i15.1632
更新日期:2018-04-21 00:00:00
abstract:AIM:To discuss the use of mycophenolate mofetil (MMF) as an immunosuppressant in steroid resistant rejection after liver transplantation. METHODS:The clinical records of 260 adult patients who underwent living donor liver transplantation (LDLT) were reviewed. Tacrolimus and methylprednisolone were used for primary imm...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i30.4870
更新日期:2006-08-14 00:00:00