Pathogenic effects of Opolysaccharide from Shigella flexneri strain.

Abstract:

:AIM:To investigate the specific pathogenesis of O-polysaccharide (O-PS) which is on the outer membrane of lipopolysaccharides (LPS) from Shigella flexneri.METHODS:The O-PS was isolated and purified from Shigella flexneri 5 M90T by enzymatic hydrolysis and gel chromatography. Effects of O-PS were observed by in vitro experiment, (HeLa cell culture), and in vivo experiment (rabbit ileal loop assay).RESULTS:In vitro and in vivo experiments with the purified O-PS from Shigella flexneri revealed that the O-PS alone was toxic to Hela cells and caused mucosal inflammation and hemorrhagic exudation in ileal loop of rabbit.DISCUSSION:O-PS might be one of the factors causing diarrhea and its mechanism was different from endotoxin reaction of LPS.The molecular mechanism of O-PS need further studies.

journal_name

World J Gastroenterol

authors

Zhong QP

doi

10.3748/wjg.v5.i3.245

keywords:

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

245-248

issue

3

eissn

1007-9327

issn

2219-2840

journal_volume

5

pub_type

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