Bovine explant model of degeneration of the intervertebral disc.

Abstract:

BACKGROUND:Many new treatments for degeneration of the intervertebral disc are being developed which can be delivered through a needle. These require testing in model systems before being used in human patients. Unfortunately, because of differences in anatomy, there are no ideal animal models of disc degeneration. Bovine explant model systems have many advantages but it is not possible to inject any significant volume into an intact disc. Therefore we have attempted to mimic disc degeneration in an explant bovine model via enzymatic digestion. METHODS:Bovine coccygeal discs were incubated with different concentrations of the proteolytic enzymes, trypsin and papain, and maintained in culture for up to 3 weeks. A radio-opaque solution was injected to visualise cavities generated. Degenerative features were monitored histologically and biochemically (water and glycosaminoglycan content, via dimethylmethylene blue). RESULTS AND CONCLUSION:The central region of both papain and trypsin treated discs was macro- and microscopically fragmented, with severe loss of metachromasia. The integrity of the surrounding tissue was mostly in tact with cells in the outer annulus appearing viable. Biochemical analysis demonstrated greatly reduced glycosaminoglycan content in these compared to untreated discs. We have shown that bovine coccygeal discs, treated with proteolytic enzymes can provide a useful in vitro model system for developing and testing potential new treatments of disc degeneration, such as injectable implants or biological therapies.

authors

Roberts S,Menage J,Sivan S,Urban JP

doi

10.1186/1471-2474-9-24

subject

Has Abstract

pub_date

2008-02-25 00:00:00

pages

24

issn

1471-2474

pii

1471-2474-9-24

journal_volume

9

pub_type

杂志文章
  • Hyaluronic acid affects the in vitro induction effects of synthetic PAMPS and PDMAAm hydrogels on chondrogenic differentiation of ATDC5 cells, depending on the level of concentration.

    abstract:BACKGROUND:It has been a common belief that articular cartilage tissue cannot regenerate in vivo. Recently, however, we have found that spontaneous hyaline cartilage regeneration can be induced in vivo by implanting a synthetic double-network (DN) hydrogel, which is composed of poly-(2-acrylamido-2-methylpropanesulfoni...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-14-56

    authors: Yoshikawa K,Kitamura N,Kurokawa T,Gong JP,Nohara Y,Yasuda K

    更新日期:2013-02-05 00:00:00

  • Functional assessments of foot strength: a comparative and repeatability study.

    abstract:BACKGROUND:Evaluating the strength of the small muscles of the foot may be useful in a variety of clinical applications but is challenging from a methodology standpoint. Previous efforts have focused primarily on the functional movement of toe flexion, but clear methodology guidelines are lacking. A novel foot doming t...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12891-019-2981-6

    authors: Bruening DA,Ridge ST,Jacobs JL,Olsen MT,Griffin DW,Ferguson DH,Bassett KE,Johnson AW

    更新日期:2019-12-14 00:00:00

  • Are viruses associated with disc herniation? A clinical case series.

    abstract:BACKGROUND:There is some limited evidence for the presence of viruses in herniated disc material including a previous case series that claimed to provide "unequivocal evidence of the presence of herpes virus DNA in intervertebral disc specimens of patients with lumbar disc herniation suggesting the potential role of he...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-020-3052-8

    authors: Walker BF,Armson AJ,O'Dea MA,White JR,Lind CRP,Woodland PR

    更新日期:2020-01-14 00:00:00

  • Factors associated with diagnostic stage of hip osteoarthritis due to acetabular dysplasia among Japanese female patients: a cross-sectional study.

    abstract:BACKGROUND:In Japan, the majority of hip osteoarthritis (OA) was caused by acetabular dysplasia, and about 90 % of patients were female. The present study focused on Japanese female patients with hip OA due to acetabular dysplasia, and examined the associated factors with OA staging at diagnosis, in special reference t...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-016-1179-4

    authors: Ohfuji S,Jingushi S,Kondo K,Sofue M,Itoman M,Matsumoto T,Hamada Y,Shindo H,Takatori Y,Yamada H,Yasunaga Y,Ito H,Mori S,Owan I,Fujii G,Ohashi H,Takahashi S,Hirota Y

    更新日期:2016-08-02 00:00:00

  • Individual and occupational risk factors for knee osteoarthritis - study protocol of a case control study.

    abstract:BACKGROUND:Knee osteoarthritis (OA) is one of the frequent and functionally impairing disorders of the musculoskeletal system. In the literature, a number of occupational risk factors are discussed as being related to the development and progress of knee joint diseases, e.g. working in kneeling or squatting posture, li...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-9-26

    authors: Klussmann A,Gebhardt H,Liebers F,von Engelhardt LV,Dávid A,Bouillon B,Rieger MA

    更新日期:2008-02-26 00:00:00

  • Protocol for work place adjusted intelligent physical exercise reducing musculoskeletal pain in shoulder and neck (VIMS): a cluster randomized controlled trial.

    abstract:BACKGROUND:Neck and shoulder complaints are common among employees in sedentary occupations characterized by intensive computer use. Specific strength training is a promising type of physical exercise for relieving neck and shoulder pain in office workers. However, the optimal combination of frequency and exercise dura...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1471-2474-11-173

    authors: Andersen LL,Zebis MK,Pedersen MT,Roessler KK,Andersen CH,Pedersen MM,Feveile H,Mortensen OS,Sjøgaard G

    更新日期:2010-08-05 00:00:00

  • Analysis of the correlative factors in the selection of interbody fusion cage height in transforaminal lumbar interbody fusion.

    abstract:BACKGROUND:Selecting an interbody cage with appropriate height is one of the key steps in lumbar interbody fusion, and has an important impact on clinical efficacy. How to choose the appropriate height of the cage becomes one of the core problems of lumbar interbody fusion for spine surgeons. However, studies about obj...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-016-0866-5

    authors: Wang H,Chen W,Jiang J,Lu F,Ma X,Xia X

    更新日期:2016-01-12 00:00:00

  • Teriparatide use during an economic crisis: baseline data from the Greek cohort of the Extended Forsteo Observational Study (ExFOS).

    abstract:BACKGROUND:The Extended Forsteo Observational Study (ExFOS) is a multinational, non-interventional, prospective, observational study that aims to provide real-life data on patients with osteoporosis treated with teriparatide for up to 24 months. It includes the new indications of osteoporosis in men and glucocorticoid-...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12891-015-0600-8

    authors: Aloumanis K,Kapetanos G,Bartzis N,Drossinos V,Hellenic ExFOS study group.

    更新日期:2015-06-05 00:00:00

  • Rheumatoid arthritis and the incidence of influenza and influenza-related complications: a retrospective cohort study.

    abstract:BACKGROUND:Patients with rheumatoid arthritis (RA) are known to be at increased risk of infection, particularly if they are taking drugs with immunomodulatory effects. There is a need for more information on the risk of influenza in patients with RA. METHODS:A retrospective cohort study was carried out using data gath...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-13-158

    authors: Blumentals WA,Arreglado A,Napalkov P,Toovey S

    更新日期:2012-08-27 00:00:00

  • Participation in physical activity in patients 1-4 years post total joint replacement in the Dominican Republic.

    abstract:BACKGROUND:To address both the growing burden of joint disease and the gaps in medical access in developing nations, medical relief organizations have begun to launch programs to perform total joint replacement (TJR) on resident populations in developing countries. One outcome of TJR of particular interest is physical ...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-15-207

    authors: Elman SA,Dong Y,Stenquist DS,Ghazinouri R,Alcantara L,Collins JE,Beagan C,Thornhill TS,Katz JN

    更新日期:2014-06-16 00:00:00

  • An interdisciplinary intervention for older Taiwanese patients after surgery for hip fracture improves health-related quality of life.

    abstract:BACKGROUND:The effects of intervention programs on health-related quality of life (HRQOL) of patients with hip fracture have not been well studied. We hypothesized that older patients with hip fracture who received our interdisciplinary intervention program would have better HRQOL than those who did not. METHODS:A ran...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1471-2474-11-225

    authors: Shyu YI,Liang J,Wu CC,Cheng HS,Chen MC

    更新日期:2010-09-29 00:00:00

  • Longitudinal analysis of vertebral fracture and BMD in a Canadian cohort of adult cystic fibrosis patients.

    abstract:BACKGROUND:Vertebral fractures in patients with cystic fibrosis (CF) may contribute to an accelerated decline in lung function and can be a contraindication to lung transplantation. In this study, we examined longitudinal change in bone mineral density (BMD) and the prevalence of vertebral fractures in adult CF patient...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-9-125

    authors: Papaioannou A,Kennedy CC,Freitag A,O'Neill J,Pui M,Ioannidis G,Webber C,Pathak A,Hansen S,Hennessey R,Adachi JD

    更新日期:2008-09-19 00:00:00

  • Motor unit number index detects the effectiveness of surgical treatment in improving distal motor neuron loss in patients with incomplete cervical spinal cord injury.

    abstract:BACKGROUND:Recovery of motor dysfunction is important for patients with incomplete cervical spinal cord injury (SCI). To enhance the recovery of muscle strength, both research and treatments mainly focus on injury of upper motor neurons at the direct injury site. However, accumulating evidences have suggested that SCI ...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-020-03567-w

    authors: Li J,Zhu Y,Li Y,He S,Wang D

    更新日期:2020-08-15 00:00:00

  • Heart rate variability in sciatica patients referred to spine surgery: a case control study.

    abstract:BACKGROUND:A chronic pain condition may result in altered autonomic nervous system regulation in various patient populations. We evaluated whether autonomic regulation differs between sciatica patients referred to spine surgery and age-matched healthy controls analyzed with heart rate variability techniques (HRV). MET...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-14-149

    authors: Södervall J,Karppinen J,Puolitaival J,Kyllönen E,Kiviniemi AM,Tulppo MP,Hautala AJ

    更新日期:2013-04-26 00:00:00

  • Back complaints in the elders (BACE); design of cohort studies in primary care: an international consortium.

    abstract:BACKGROUND:Although back complaints are common among older people, limited information is available in the literature about the clinical course of back pain in older people and the identification of older persons at risk for the transition from acute back complaints to chronic back pain. The aim of this study is to ass...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,多中心研究

    doi:10.1186/1471-2474-12-193

    authors: Scheele J,Luijsterburg PA,Ferreira ML,Maher CG,Pereira L,Peul WC,van Tulder MW,Bohnen AM,Berger MY,Bierma-Zeinstra SM,Koes BW

    更新日期:2011-08-19 00:00:00

  • Anti-infliximab antibodies are already detectable in most patients with rheumatoid arthritis halfway through an infusion cycle: an open-label pharmacokinetic cohort study.

    abstract:BACKGROUND:This study in patients with rheumatoid arthritis (RA) treated with infliximab describes prospectively the course of (anti)infliximab levels within an infusion cycle to assess at what moment patients develop low/no infliximab trough levels and/or detectable anti-infliximab levels. METHODS:Infliximab treated ...

    journal_title:BMC musculoskeletal disorders

    pub_type: 临床试验,杂志文章

    doi:10.1186/1471-2474-12-12

    authors: van den Bemt BJ,den Broeder AA,Wolbink GJ,Hekster YA,van Riel PL,Benraad B,van den Hoogen FH

    更新日期:2011-01-13 00:00:00

  • Incidence and characteristics of distal radius fractures in a southern Swedish region.

    abstract:BACKGROUND:The incidence of distal radius fracture has increased substantially during the last 50 years according to several studies that estimated the overall incidence in various general populations. The incidence of fracture classified according to severity has not been well documented. The aim of this population-ba...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-8-48

    authors: Brogren E,Petranek M,Atroshi I

    更新日期:2007-05-31 00:00:00

  • What is the impact of knee morphology on posterior cruciate ligament avulsion fracture in men and women: a case control study.

    abstract:BACKGROUND:Several studies on the relationship between morphological parameters and traumatic diseases of the knee have already been conducted. However, few studies focused on the association between knee morphology and posterior cruciate ligament (PCL) avulsion fracture in adults. The objective of this study was to ev...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-021-03984-5

    authors: Fan N,Zheng YC,Zang L,Yang CG,Yuan S,Du P,Liu YM,Zhao Q,Wang JW

    更新日期:2021-01-21 00:00:00

  • Bone mineral density by digital X-ray radiogrammetry is strongly decreased and associated with joint destruction in long-standing rheumatoid arthritis: a cross-sectional study.

    abstract:BACKGROUND:The aims were to explore bone mineral density (BMD) by digital X-ray radiogrammetry (DXR) in postmenopausal women with long-lasting rheumatoid arthritis (RA) in relation to dual x-ray absorptiometry (DXA)-BMD, joint destruction by conventional radiographs and disease related variables in a cross-sectional st...

    journal_title:BMC musculoskeletal disorders

    pub_type: 临床试验,杂志文章

    doi:10.1186/1471-2474-12-242

    authors: Forsblad-d'Elia H,Carlsten H

    更新日期:2011-10-24 00:00:00

  • Outcomes and early revision rate after medial unicompartmental knee arthroplasty: prospective results from a non-designer single surgeon.

    abstract:BACKGROUND:This prospective study evaluates outcomes and reoperation rates for unicompartmental knee arthroplasty (UKA) from a single non-designer surgeon using relatively extended criteria of degenerative changes of grade 2 or above in either or both non-operated compartments. METHODS:187 consecutive medial mobile be...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-018-2099-2

    authors: Hutt JRB,Sur A,Sur H,Ringrose A,Rickman MS

    更新日期:2018-05-29 00:00:00

  • Minimal important improvement thresholds for the six-minute walk test in a knee arthroplasty cohort: triangulation of anchor- and distribution-based methods.

    abstract:BACKGROUND:The 6-minute walk test (6MWT) is a commonly used metric for measuring change in mobility after knee arthroplasty, however, what is considered an improvement after surgery has not been defined. The determination of important change in an outcome assessment tool is controversial and may require more than one a...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12891-016-1249-7

    authors: Naylor JM,Mills K,Buhagiar M,Fortunato R,Wright R

    更新日期:2016-09-13 00:00:00

  • Modifications to advanced Core decompression for treatment of Avascular necrosis of the femoral head.

    abstract:BACKGROUND:"Advanced Core Decompression" (ACD) is a new technique for treatment of osteonecrosis of the femoral head (ONFH) that includes removal of the necrotic tissue using a percutaneous expandable reamer followed by refilling of the drill hole and the defect with an injectable, hard-setting, composite calcium sulph...

    journal_title:BMC musculoskeletal disorders

    pub_type: 临床试验,杂志文章

    doi:10.1186/s12891-017-1811-y

    authors: Landgraeber S,Warwas S,Claßen T,Jäger M

    更新日期:2017-11-21 00:00:00

  • The outcomes of hip resurfacing compared to standard primary total hip arthroplasty in Men.

    abstract:BACKGROUND:The purpose of this study was to evaluate the clinical and radiographic outcomes of hip resurfacing patients and compare them to standard primary total hip arthroplasty procedures performed during the same period of time. METHODS:One hundred and fourteen consecutive men who had a mean age of 50 years (range...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-14-161

    authors: Issa K,Palich A,Tatevossian T,Kapadia BH,Naziri Q,Mont MA

    更新日期:2013-05-08 00:00:00

  • Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration.

    abstract:BACKGROUND:Intervertebral disc degeneration (IVDD) is a primary cause of degenerative disc diseases; however, the mechanisms underlying the degeneration remain unclear. The immunoinflammatory response plays an important role in IVDD progression. The inflammatory cytokine lymphotoxin-α (LTα), formerly known as TNFβ, is ...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-020-03934-7

    authors: Guo Z,Qiu C,Mecca C,Zhang Y,Bian J,Wang Y,Wu X,Wang T,Su W,Li X,Zhang W,Chen B,Xiang H

    更新日期:2021-01-13 00:00:00

  • Clinical and radiographic outcomes of the treatment of adolescent idiopathic scoliosis with segmental pedicle screws and combined local autograft and allograft bone for spinal fusion: a retrospective case series.

    abstract:BACKGROUND:High morbidity has been reported with iliac crest bone graft harvesting; however, donor bone is typically necessary for posterior spinal fusion. Autograft bone combined with allograft may reduce the morbidity associated with iliac crest bone harvesting and improve the fusion rate. Our aim in this study was t...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-11-159

    authors: Yang X,Xu H,Li M,Gu S,Fang X,Wang J,Ni J,Wu D

    更新日期:2010-07-14 00:00:00

  • Exposure to animals and risk of oligoarticular juvenile idiopathic arthritis: a multicenter case-control study.

    abstract:BACKGROUND:An inverse association between early contact with microbial compounds and respiratory allergies is well established. The protective effect of infant contact with animals was also shown for inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE). We aimed to test the association between animal...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,多中心研究

    doi:10.1186/1471-2474-11-73

    authors: Radon K,Windstetter D,Poluda D,Häfner R,Thomas S,Michels H,von Mutius E

    更新日期:2010-04-20 00:00:00

  • Medical decision-making among Hispanics and non-Hispanic Whites with chronic back and knee pain: a qualitative study.

    abstract:BACKGROUND:Musculoskeletal disorders affect all racial and ethnic groups, including Hispanics. Because these disorders are not life-threatening, decision-making is generally preference-based. Little is known about whether Hispanics in the U.S. differ from non-Hispanic Whites with respect to key decision making preferen...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/1471-2474-12-78

    authors: Katz JN,Lyons N,Wolff LS,Silverman J,Emrani P,Holt HL,Corbett KL,Escalante A,Losina E

    更新日期:2011-04-21 00:00:00

  • Associations between body composition measurements of obesity and radiographic osteoarthritis in older adults: Data from the Dong-gu Study.

    abstract:BACKGROUND:We examined the effects of fat deposition on radiographic osteoarthritis (OA) to determine the role of obesity in the pathogenesis of radiographic OA. METHODS:Data were taken from the Dong-gu cohort, a cross-sectional study of 2,367 subjects. Baseline characteristics, waist circumference (WC), waist-to-hip ...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-016-1040-9

    authors: Wen L,Kang JH,Yim YR,Kim JE,Lee JW,Lee KE,Park DJ,Kim TJ,Park YW,Kweon SS,Lee YH,Yun YW,Shin MH,Lee SS

    更新日期:2016-04-29 00:00:00

  • Functioning and equality according to International Classification of Functioning, Disability and Health (ICF) in people with skeletal dysplasia compared to matched control subjects - a cross-sectional survey study.

    abstract:BACKGROUND:Skeletal dysplasias are rare disorders often leading to severe short stature. This study aimed to gain new comprehensive information about functioning and equality in people affected by skeletal dysplasia compared to matched controls without skeletal dysplasia. METHODS:Functioning was assessed by questionna...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章

    doi:10.1186/s12891-020-03835-9

    authors: Hyvönen H,Anttila H,Tallqvist S,Muñoz M,Leppäjoki-Tiistola S,Teittinen A,Mäkitie O,Hiekkala S

    更新日期:2020-12-04 00:00:00

  • Effects of rehabilitation approaches for runners with patellofemoral pain: protocol of a randomised clinical trial addressing specific underlying mechanisms.

    abstract:BACKGROUND:Patellofemoral pain (PFP) is highly prevalent in runners, and often leads to functional limitations and cessation of running. Training errors as well as decreased lower limb strength and control during running have all been associated with PFP. While strengthening and gait retraining are commonly used by cli...

    journal_title:BMC musculoskeletal disorders

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12891-015-0859-9

    authors: Esculier JF,Bouyer LJ,Dubois B,Frémont P,Moore L,Roy JS

    更新日期:2016-01-06 00:00:00