Abstract:
:We investigated cell susceptibility to hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines (RYSTs) and attempted to correlate this with the known potentially relevant molecular determinants of apoptosis, p53 protein status, Bcl-2 family of proteins and heat shock proteins (Hsp). Parent cell line, NMT-1 (carrying wild-type p53 gene) was radiosensitive but thermoresistant compared to the variant cell line, NMT-1R (mutated type p53), which was isolated from NMT-1 by repeated radiation exposure. Induction of apoptosis by hyperthermia at 43 degrees C was morphologically detected in both RYSTs using hematoxylin and eosin, and TUNEL staining and additionally confirmed by DNA ladder formation (the cleavage of DNA into oligonucleosomal fragments). Western blot analysis showed an increase in expression of p53, p21WAF1/CIP1, Hsp70 proteins in both cell lines after heat-shock at 43 degrees C for 30 min. Hsp90 expression increased in NMT-1 but was not affected by heating in NMT-1R cells, whereas hyperthermia exerted no effect on the endogenous expression of Bax. Bcl-2 protein could not be detected in either RYST. These results suggest that hyperthermia induced apoptosis in both NMT-1 and NMT-1R and apoptosis in RYSTs may be independent of p53-dependent signaling pathway.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Islam MS,Mitsuhashi N,Akimoto T,Sakurai H,Hasegawa M,Ishikawa H,Niibe Hdoi
10.3892/or.8.3.501keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
501-7issue
3eissn
1021-335Xissn
1791-2431journal_volume
8pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::PSMB4, proteasome subunit β4, is a member of the ubiquitin-proteasome family, and is elevated in a variety of malignancies. However, the expression level and related mechanism of PSMB4 in breast cancer remains unclear. Therefore, the present study investigated the expression level of PSMB4 in eight pairs of breast can...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6588
更新日期:2018-10-01 00:00:00
abstract::CXC chemokine receptor 4 (CXCR4) is associated with poor clinical outcomes and decreased survival in hepatocellular carcinoma (HCC). In the present study, we targeted CXCR4 by CRISPR/Cas9 in HepG2 cells and observed the effects both in vitro and in vivo. The results indicated that after targeting CXCR4 the expression ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5601
更新日期:2017-06-01 00:00:00
abstract::Toll-like receptor (TLR)-mediated signaling induces cell migration or invasion in several tumors and various stages of cancer. Interactions of mesothelin, a 40-kDa cell surface glycoprotein, with cancer antigen 125 (CA125) is associated with drug resistance, metastasis, and poor clinical outcome of ovarian cancer pati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5941
更新日期:2017-11-01 00:00:00
abstract::In breast cancer, neuregulin-1 (NRG1) is known as a ligand for the HER3 receptor, which has no intrinsic tyrosine kinase activity. When activated by NRG1 binding, the HER3 receptor forms a heterodimer with other HER family receptors and mediates downstream signaling pathways, leading to multiple effects including grow...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3330
更新日期:2014-09-01 00:00:00
abstract::External abdomino-pelvic irradiation after primary surgery in early stages of epithelial ovarian carcinoma has been used as adjuvant therapy. The aims of this study were to evaluate efficacy and tolerability of abdomino-pelvic radiotherapy in ovarian carcinomas and to find predictive factors for recurrent disease. Fro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::Actin has been studied extensively for decades, but so far its well-defined role has been found localized to the cytoplasm. The present study characterizes the distribution pattern of actin in the nucleus of HL-60 and K-562 cell lines treated with etoposide and doxorubicin. F-actin labeled with TRITC-phalloidin was fo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract::Oral mucositis is a severe complication of radiotherapy. Hence, it may constitute a serious medical safety risk for astronauts during extended space flights, such as missions to Mars, during which they are exposed to heavy-ion irradiation. For risk assessment of developing radiation-induced mucositis, a three-dimensio...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3441
更新日期:2014-11-01 00:00:00
abstract::Aberrant nuclear factor-erythroid 2 (NRF2) expression correlates with tumor development. We investigated NRF2 expression in ovarian epithelial carcinoma (OEC), aiming to identify associations with clinicopathological factors, hormones and induced reactive oxygen species (ROS). Immun...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1700
更新日期:2012-06-01 00:00:00
abstract::Monoclonal antibody therapy for immune checkpoint blockade has achieved promising results for several types of malignant tumors. For the future treatment of gastrointestinal stromal tumors (GISTs) by immune checkpoint blockade, expression of immune checkpoint-related molecules that suppress antitumor immunity in GISTs...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4149
更新日期:2015-10-01 00:00:00
abstract::We studied the role of chemotherapy in cancer cachexia, which is well known to lower QOL and responsiveness to chemotherapy. In BALB/c mice we used two clones derived from the murine colon 26 adenocarcinoma cell line; clone 5, a non-cachexigenic tumor and clone 20, a cachexigenic tumor, in which IL-6 mRNA was selectiv...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::We conducted a clinical pharmacological evaluation of paclitaxel/carboplatin combination in Japanese patients with non-small cell lung cancer. The purpose of this study was to identify the optimal dose of this combination and to investigate the relationships between the pharmacokinetic profiles of these 2 drugs, serum...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a common malignant cancer worldwide characterized by high metastatic potential and poor prognosis following radical resection. Metastasin is a Ca(2+)-binding protein associated with tumor metastasis. However, the expression and function of metastasin remain unknown. In the present stu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2341
更新日期:2013-05-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) patients with epithelial growth factor receptor (EGFR) mutations and bone metastases are often concurrently administered tyrosine kinase inhibitors (TKIs) and bisphosphonates. Yet, the effects and mechanisms of these agents are unclear. In the present study, we aimed to ascertain whe...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4741
更新日期:2016-06-01 00:00:00
abstract::HIF-1 is a heterodimer consisting of the HIF-1alpha and HIF-1beta subunits, and HIF-1alpha is the unique oxygen regulated subunit that determines HIF-1 activity. HIF-1alpha upgrades many gene products which include the glucose transporter protein 1 (Glut-1). Immunohistochemical studies using a monoclonal antibody spec...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.14.4.909
更新日期:2005-10-01 00:00:00
abstract::lncRNA metastasis-associated lung adencarcinoma transcript 1 (MALAT1) plays an important role in the metastasis of lung cancer. Yet, its role in bone metastasis and the related mechanism remain unknown. The present study aimed to investigate the role of lncRNA MALAT1 in the bone metastasis of non-small cell lung cance...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4909
更新日期:2016-09-01 00:00:00
abstract::The aim of the present study was to discover the signaling pathways associated with 2-(3-hydroxy-5-methoxy-phenyl)-6,7-methylenedioxyquinolin-4-one (YYK1)-induced apoptosis in HL-60 human leukemia cells. YYK1 induced cytotoxic effects, cell morphological changes, decreased the cell ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1923
更新日期:2012-10-01 00:00:00
abstract::Malignant peripheral nerve tumours (MPNT), e.g. malignant schwannoma, represent a relatively rare tumour entity which is usually regarded as a member of the group of soft-tissue sarcomas and treated accordingly. For chemotherapeutic approaches in metastasised disease, ifosfamide and doxorubicin have been identified as...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Given such differences as relative tumor burden, the optimal dose and schedule for postoperative adjuvant chemotherapy of microscopic disease might be expected to differ significantly from therapy of advanced higher volume disease. We investigated this hypothesis by determining the optimal dose and schedule of the 5-F...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract::Although antiestrogens significantly improve the survival of patients with ER-positive breast cancer, therapeutic resistance remains a major limitation. The combinatorial use of antiestrogen with other therapies was proposed to increase their efficiency and more importantly, to prevent or delay the resistance phenomen...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4873
更新日期:2016-08-01 00:00:00
abstract::Withaferin A (Wit A), a steroidal lactone isolated from Withania somnifera, exhibits anti-inflammatory, immuno-modulatory and anti-angiogenic properties and antitumor activities. In the present study, we investigated the effects of Wit A on protease-mediated invasiveness of the human metastatic cancer cell lines Caski...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2487
更新日期:2013-08-01 00:00:00
abstract::All-trans retinoic acid (ATRA) is a potent chemopreventive and therapeutic agent and exerts its effects by inducing growth arrest. In the present study, we demonstrated that ATRA activated the expression of p53 via Axin and induced cell cycle arrest at the G1/S phase and apoptosis of glioma cells. Briefly, C6 cells we...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2391
更新日期:2013-06-01 00:00:00
abstract::To investigate whether the expression of Topoisomerase II-alpha (TII-alpha) can serve as a prognostic factor in renal cell carcinoma (RCC), histological sections from 27 renal tumors were stained immunohistochemically for TII-alpha expression. The percentage of positive cells in the area of greatest staining was recor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::In this study, we demonstrate an important role of activation of the hypoxia-inducible factor-1 (HIF-1) pathway in endometrial carcinogenesis and tumor phenotype development of endometrial carcinoma, and suggest a unique role of the HIF-1-target gene, differentiated embryo chondrocyte 2 (DEC2), in carcinogenesis. Hypo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-04-01 00:00:00
abstract::The aim of the present study was to evaluate the antitumor effects of the curcumin analogue GL63 on radioresistant nasopharyngeal carcinoma (NPC) CNE2R cells and parental CNE2 cells. The cell viability and proliferation of NPC cells were detected by MTT assay and colony formation assay. The suppressive effect on tumor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2457
更新日期:2013-07-01 00:00:00
abstract::Invasiveness and metastasis may seriously affect the prognosis of small cell lung cancer (SCLC). In the present study, we analyzed the effects and inherent mechanisms of action of polysialic acid-modified neural cell adhesion molecule (NCAM) on the invasive and metastatic potential of SCLC. Gene transfection and short...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5279
更新日期:2017-01-01 00:00:00
abstract::Cyclooxygenase-2 (COX-2) and its metabolite prostaglandin E2 (PGE2) can potentially affect most of the events in cancer development, including promotion of proliferation, resistance to apoptosis, angiogenesis, immune suppression and invasion. However, worldwide attention has predominantly centered on the cardiovascula...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3543
更新日期:2014-12-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT) plays an important role in the development of tumors and has been investigated as a potent target for anticancer therapy. In the present study, we constructed a recombinant adenovirus, Ad-EGFP-C197 which was capable of expressing COOH‑terminal polypeptide of hTERT (amino ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4298
更新日期:2015-12-01 00:00:00
abstract::The aim of the study was to compare cross sections with longitudinal sections in histopathological examination of the rat heart after irradiation, to find the most optimal method for the detection of cardiac radiation injuries. For this purpose, rats were irradiated locally on the heart with a single dose of 0 or 20 G...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.12.2.213
更新日期:2004-08-01 00:00:00
abstract::The long non‑coding RNA (lncRNA) PVT1 plays vital roles in the tumorigenesis and development of various types of cancer. However, the potential expression profiling, functions and pathways of PVT1 in HCC remain unknown. PVT1 was knocked down in SMMC‑7721 cells, and a miRNA microarray analysis was performed to detect t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6410
更新日期:2018-07-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) endows cancer cells with enhanced invasive and metastatic potential during cancer progression. Fractalkine, also known as chemokine (C-X3-C motif) ligand 1 (CX3CL1), the only member recognized so far that belongs to the CX3C chemokine subfamily, was reported to participate in...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4470
更新日期:2016-02-01 00:00:00