Effects of nerve growth factor on intracellular free Ca2+ in oxygen/glucose-deprived cultures from cerebral cortex of fetal rats.

Abstract:

OBJECTIVE:To explore the involvement of calcium in the mechanism of hypoxic/hypoglycemic insults and nerve growth factor (NGF) protection. METHODS:Neuron viability and lactate dehydrogenase (LDH) efflux in the bathing medium in cerebral cortex cultures from fetal rats were measured. Calcium fluorescent indicator Fura-2/AM was used to measure the intracellular free calcium ([Ca2+]i). RESULTS:Massive neuronal death occurred 16-24 h following the onset of hypoxia/hypoglycemia. NGF (3-100 micrograms.L-1) dose-dependently attenuated 24 h hypoxia/hypoglycemia-induced efflux of LDH and elevated the number of surviving neurons. Hypoxia/hypoglycemia induced a reduction in [Ca2+]i in early stage and a large elevation of [Ca2+]i in later period. NGF 50 micrograms.L-1 increased [Ca2+]i to normal limits during the early stages of hypoxia/hypoglycemia and prevented the later elevation in [Ca2+]i. CONCLUSION:The [Ca2+]i elevation may be responsible for the cell damage in oxygen/glucose deprived cultures. NGF protects cerebral cortical neurons against hypoxic/hypoglycemic insults via stabilizing [Ca2+]i level or preventing the late rise in [Ca2+]i.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Wu J,Zhang J

keywords:

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

1031-4

issue

11

eissn

0366-6999

issn

2542-5641

journal_volume

111

pub_type

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