Abstract:
:Using immunocytochemistry at light- and electron-microscope levels, we studied the distribution of three monoclonal antibodies (AE1, AE2, AE3) specific for mammalian alpha-keratins in regenerating lizard epidermis. We also characterized the keratins expressed during this process by immunoblotting after electrophoretic separation. The AE1 antibody is localized in the basal and suprabasal layers of prescaling and scaling epidermis. During the first stages of scale neogenesis, the AE1 antibody also marks the differentiating oberhautchen and beta-layer, but it disappears from these layers as they mature. This antibody does not stain the prekeratinized and keratinized outermost layers in the hinge region. The AE2 antibody labels the superficial wound epidermis, prekeratinizing and keratinized beta- and alpha-layers, but not basal and suprabasal cells. The AE3 antibody labels all living and keratinized epidermal layers, although AE3 immunoreactivity decreases and disappears as the beta-layer matures. The ultrastructural study shows that the AE2 and AE3, but not the AE1, antibodies specifically label small electron-dense areas within the beta-layer, suggesting retention of alpha-keratins. In the stages of tail regeneration examined, immunoblotting with the three antibodies used for the immunolocalization gives a pattern similar to that of the normal epidermis, except distally, where the process of scale differentiation begins. In this region, in addition to the keratin forms discovered in the normal and in proximal regenerating epidermis, an intense low molecular weight band at 40-41 kDa, positive to all three antibodies, is clearly detectable. Furthermore, in the distal region AE1 and AE3 antibodies, but not the AE2, recognize a weak band at 77-78 kDa not present in the normal and proximal epidermis. The localization and the possible role of the different keratins in the regenerating epidermis is discussed.
journal_name
J Morpholjournal_title
Journal of morphologyauthors
Alibardi L,Maurizii MG,Taddei Cdoi
10.1002/1097-4687(200012)246:3<179::AID-JMOR2>3.0.keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
179-91issue
3eissn
0362-2525issn
1097-4687pii
10.1002/1097-4687(200012)246:3<179::AID-JMOR2>3.0.journal_volume
246pub_type
杂志文章abstract::For better understanding of the links between limb morphology and the metabolic cost of locomotion, we have characterized the relationships between limb length and shape and other functionally important variables in the straightened forelimbs and hindlimbs of a sample of 12 domestic dogs (Canis familiaris). Intra-anim...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4687(199711)234:2<183::AID-JMOR
更新日期:1997-11-01 00:00:00
abstract::The masticatory pattern of Sphenodon punctatus, the sole remaining rhynchocephalian, now restricted to islands off the coast of New Zealand, has been analyzed by detailed anatomy, cinematography, cinefluoroscopy, and electromyography. Food reduction consists of a closing, crushing bite followed by a propalineal slidin...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051710307
更新日期:1982-03-01 00:00:00
abstract::The medaka, Oryzias latipes, is a model organism in experimental biology. Its ontogeny and osteology have been studied frequently, but no detailed description of its cranial anatomy exists. Based on manual dissections, histological slide sections, and 3D-reconstructions, we describe the anatomy of the jaw apparatus of...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10746
更新日期:2009-09-01 00:00:00
abstract::The apical area of larvae of four primitive pulmonate species was investigated by means of serial ultrathin and light microscope sections. Cephalic sensory organs (CSOs) were found in the larvae of Onchidium cf. branchiferum (Onchidiidae) and Laemodonta octanfracta (Ellobiidae), while no trace of the organ was present...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1076
更新日期:2002-01-01 00:00:00
abstract::The female reproductive system of Sphaerodema rusticum consists of a pair of ovaries, two lateral oviducts, a median common oviduct, and a median spermatheca. Accessory glands are absent. Each ovary has five free ovarioles branching from the oviduct. Each ovariole consists of a terminal filament, germarium, vitellariu...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052050207
更新日期:1990-08-01 00:00:00
abstract::The mouthparts of female Corethrella brakeleyi and C. wirthi were studied using light and electron microscopy. Mandibles, hypopharynx and labium are highly sclerotized and are modified for obtaining blood meals. All structures were larger in C. brakeleyi than in C. wirthi except mandibular and hypopharyngeal teeth; th...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051610204
更新日期:1979-08-01 00:00:00
abstract::The forelimbs, along with the crania, are an essential part of the prey-killing apparatus in cats. Linear morphometrics of the forelimbs were used to determine the morphological differences between felids that specialize on large prey, small prey, or mixed prey. We also compared the scaling of felid forelimbs to those...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10712
更新日期:2009-06-01 00:00:00
abstract::The coiled shell of gastropods begins as a cap-shaped lens of organic and calcified material that covers the posterior dorsal side of the larva. During development the cap enlarges to cover the larval visceral mass. Marginal growth then produces the characteristic coiled shell. One model of the initiation of shell coi...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4687(199801)235:1<77::AID-JMOR6
更新日期:1998-01-01 00:00:00
abstract::During ontogeny, the apical and basal components of dicamptodontid teeth exhibit three major developmental stages: nonpedicellate, subpedicellate, and pedicellate. Premetamorphic larvae tend to have nonpedicellate teeth, incompletely or recently metamorphosed individuals tend to have subpedicellate teeth, and fully tr...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051990204
更新日期:1989-02-01 00:00:00
abstract::Two of the forearm flexors of the horse, the superficial and deep digital flexor muscles, are critical to support the digital and fetlock joints, exhibit differing insertions, and are passively supported by the proximal and distal check ligaments, respectively. These two muscles differ in histochemical composition and...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052120306
更新日期:1992-06-01 00:00:00
abstract::The three-dimensional structure of the organ of Bellonci in the marine amphipod Gammarus setosus and the relationship between its sensory cells and concretion are described using light, transmission, and scanning electron microscopy, with chemical treatment for cell lysis, calcium chelation, glycogen staining, and lan...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051810109
更新日期:1984-07-01 00:00:00
abstract::Homolid crabs (Hypsophrys) from water deeper than 700 m in the Straits of Florida and Arabian Sea have smooth darkened oval spots contrasting with the surrounding roughened integument on inner and outer surfaces of each pincer at the base of the fixed finger. Cuticle is thinner over these spots than over surrounaing t...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051500407
更新日期:1976-12-01 00:00:00
abstract::Cover illustration. Mayetiola destructor is a major pest of wheat in Europe, North Africa and North America. In this issue of the Journal of Morphology, Schneeberg et al. (pp. 1299-1311) investigate the adult head structures of the cecidomyiid fly and compared their findings with evolutionarily less successful familie...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.20220
更新日期:2013-11-01 00:00:00
abstract::Gross lung morphology is examined in representative species from four genera within the order Lagomorpha (Lepus californicus, Sylvilagus nuttalli, Oryctolagus cuniculus, Ochotona princeps), and compared with a representative rodent out-group (Spermophilus richardonsii). Examination of pulmonary morphology reveals seve...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4687(199612)230:3<299::AID-JMOR
更新日期:1996-12-01 00:00:00
abstract::The purpose of this study was to clarify the general architecture and morphological variations of the autonomic cardiac nervous system (ACNS) in the African Cercopithecidae (Old World monkeys), and to discuss the evolutionary changes between this system in African/Asian Cercopithecidae and humans. A detailed macroscop...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10549
更新日期:2007-09-01 00:00:00
abstract::The dorsal vessel (DV) in the abdomen of the blood-feeding insect Rhodnius prolixus was divided functionally into two regions, the heart, into which haemolymph entered the DV through four pairs of ostia located in abdominal segment VII, and the aorta, along which the haemolymph was propelled from abdominal segment VI ...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052040103
更新日期:1990-04-01 00:00:00
abstract::The pineal complex of the lanterfish Triphoturus mexicans was studied by light and electron microscopy. Receptor, supportive and ganglion cells were identified in the pineal end-vesicle. The presence of receptor cells and the prominent pineal window strongly suggest a photoreceptor function. Numerous unmyelinated nerv...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::The cytoarchitecture of nuclei in the preoptic area, ventral thalamus, dorsal thalamus, epithalamus, hypothalamus, posterior tuberculum, synencephalon, and pretectum and the accessory optic nuclei was analyzed in a perciform teleost, the sea bass Dicentrarchus labrax, by using serial sections stained with cresyl-viole...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/1097-4687(200103)247:3<229::AID-JMOR1014>3
更新日期:2001-03-01 00:00:00
abstract::Scanning electron microscopy of postnatally developing gerbil vagina (birth to maturity) shows that longitudinal folds form prior to transverse folds; the process of fold formation is initiated on the dorsal wall and proceeds ventrally. From days 1 to 7 postnatally, the vaginal epithelium is composed of either flat or...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052010309
更新日期:1989-09-01 00:00:00
abstract::Head structures of the leaf mining larva of the chrysomelid species Podagricomela shirahatai are described and illustrated. Internal and external structures were reconstructed three dimensionally based on image stacks obtained with microcomputed tomography. The larval head is characterized by prognathism, a dorsoventr...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.20352
更新日期:2015-04-01 00:00:00
abstract::The orientation of the semicircular canals of the inner ear in the skull of vertebrates is one of the determinants of the capacity of this system to detect a given rotational movement of the head. Past functional studies on the spatial orientation of the semicircular canals essentially focused on the lateral semicircu...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.20665
更新日期:2017-05-01 00:00:00
abstract::Mites of the genus Veigaia are common gamasid inhabitants of forest litter. They engage in the peculiar reproductive strategy of podospermy which, along with other morphological and behavioral adaptations, involves modification of the chelicerae of the relatively rare males into gonopods. Each movable digit is provide...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10393
更新日期:2006-02-01 00:00:00
abstract::In this study, we compare and contrast armoring strategies during early ontogeny among three related species of marine turtles: the hawksbill, a species that diverged about 29 mya from the loggerhead and Kemp's ridley, which diverged from one another about 16 mya. Our purpose was to determine whether there was a corre...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.20844
更新日期:2018-09-01 00:00:00
abstract::Anseriform birds were surveyed to examine how the degree of postcranial pneumaticity varies in a behaviorally and size-diverse clade of living birds. This study attempts to extricate the relative effects of phylogeny, body size, and behavioral specializations (e.g., diving, soaring) that have been postulated to influe...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10190
更新日期:2004-08-01 00:00:00
abstract::This study examines the allometric scaling relationships of the cetacean humerus, radius, and ulna. Bone lengths and diameters were measured for 20 species of odontocete and three species of mysticete cetaceans, representing eight of the nine extant cetacean families. The scaling of individual bone proportions (bone l...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052220208
更新日期:1994-11-01 00:00:00
abstract::Light and electron microscopic techniques show that the eye of the marine prosobranch gastropod, Ilyanassa obsoleta, is composed of an optic cavity, lens, cornea, retina, and neuropile, and is surrounded by a connective tissue capsule. The adult retina is a columnar epithelium containing three morphologically distinct...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1051810207
更新日期:1984-08-01 00:00:00
abstract::The plainfin midshipman fish Porichthys notatus has both inter- and intra-sexual dimorphism in the sound-producing (vocal or sonic) muscles attached to the swimbladder wall. The "Type I" and "Type II" male morphs differ in that dramatic structural changes related to sexual maturity occur in the mass, the area of mitoc...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052160306
更新日期:1993-06-01 00:00:00
abstract::The sequential changes during spermatogenesis in the testis of two species of caecilians, Ichthyophis tricolor (Ichthyophiidae) and Uraeotyphlus cf. narayani (Uraeotyphliidae), of Western Ghats of Kerala, India, were traced using both histological techniques and transmission electron microscopy. The cell nests were as...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.10231
更新日期:2004-07-01 00:00:00
abstract::Swimming crabs of the taxon Portunoidea show specialized, paddle-shaped fifth pereiopods (P5), which play a role in these crabs' ability to swim. In this study, the morphology of the fifth pereiopod in swimming and non-swimming crabs was studied in detail and the mobility in the articulations between podomeres was cal...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.21268
更新日期:2020-12-01 00:00:00
abstract::The innervation of the musculature of the tongue and the hyobranchial apparatus of caecilians has long been assumed to be simple and to exhibit little interspecific variation. A study of 14 genera representing all six families of caecilians demonstrates that general patterns of innervation by the trigeminal, facial, g...
journal_title:Journal of morphology
pub_type: 杂志文章
doi:10.1002/jmor.1052120105
更新日期:1992-04-01 00:00:00