当前位置： SCI文献检索 > THROMBOSIS AND HAEMOSTASIS期刊下所有文献 > Sequence alignment between vWF and peptides inhibiting the vWF-collagen interaction does not result in the identification of a collagen-binding site in vWF.

Sequence alignment between vWF and peptides inhibiting the vWF-collagen interaction does not result in the identification of a collagen-binding site in vWF.

Abstract：

:We previously found that two peptides (N- and Q-peptide) selected by phage display for binding to an anti-vWF antibody, were able to inhibit vWF-binding to collagen (1). The sequence of those peptides could be aligned with the sequence in vWF at position 1129-1136 just outside the A3-domain. As the peptides represent an epitope or mimotope of vWF for binding to collagen we next wanted to study whether the alignment resulted in the identification of a new collagen binding site in vWF. We mutated the 1129-1136 VWTLPDQC sequence in vWF to VATAPAAC. Expressing this mutant vWF (7.8-vWF) in a fur-BHK cell line resulted in well processed 7.8-vWF containing a normal distribution of molecular weight multimers. However, binding studies of this mutant vWF to rat tail, human and calf skin collagens type 1, to human collagen types III and VI, revealed no decrease in vWF-binding to any of these collagens. Thus, although the N- and Q-peptides did inhibit the vWF-collagen interaction, the resulting alignment with the vWF sequence did not identify a collagen binding site, pointing out that alignments (although with a high percentage of identity) do not always result in identification of binding epitopes. However, suprisingly removal of the A3-domain or changing the vWF sequence at position 1129-1136 resulted in an increase of vWF-binding to human collagen type V1 and to rat tail collagen type 1, implying that these changes result in a different conformation of vWF with an increased binding to these collagens as a consequence.

journal_name

Thromb Haemost

journal_title

Thrombosis and haemostasis

authors

Vanhoorelbeke K,van der Plas RM,Vandecasteele G,Vauterin S,Huizinga EG,Sixma JJ,Deckmyn H

keywords:

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

621-5

issue

4

eissn

0340-6245

issn

2567-689X

pii

00100621

journal_volume

84

pub_type

杂志文章

相关文献

THROMBOSIS AND HAEMOSTASIS文献大全
  • Antithrombotic effect of MCI-9042, a new antiplatelet agent on experimental thrombosis models.

    abstract：:The antithrombotic effect of MCI-9042, (+-)-2-(dimethyl-amino)-1-((o-(m- methoxyphenethyl)phenoxyl]methyl]ethyl hydrogen succinate hydrochloride was investigated in three different experimental thrombosis models in animals. Simultaneous injection of serotonin and collagen into the tail vein in mice induced acute pulmo...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Hara H,Kitajima A,Shimada H,Tamao Y

    更新日期：1991-10-01 00:00:00

  • The HR2 haplotype of factor V: effects on factor V levels, normalized activated protein C sensitivity ratios and the risk of venous thrombosis.

    abstract：:We studied the HR2 haplotype of the factor V gene in a case-control study for venous thrombosis including 474 patients with a first deep-vein thrombosis and 474 age- and sex-matched healthy controls (Leiden Thrombophilia Study, LETS). We investigated both the original His1299Arg (A4070G) polymorphism and the Met385Thr...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： de Visser MC,Guasch JF,Kamphuisen PW,Vos HL,Rosendaal FR,Bertina RM

    更新日期：2000-04-01 00:00:00

  • A randomized and blinded comparison of three bleeding time techniques: the Ivy method, and the Simplate II method in two directions.

    abstract：:We compared the Ivy bleeding time method and two alternatives of the Simplate II method (incisions in horizontal and vertical direction) with each other, with regard to the sensitivity, the specificity, the costs and the burden for the patient. In the aspirin study an aspirin-induced bleeding defect was used. Seventy-...

    journal_title：Thrombosis and haemostasis

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：

    authors： Srámek R,Srámek A,Koster T,Briët E,Rosendaal FR

    更新日期：1992-05-04 00:00:00

  • Effect of tissue factor pathway inhibitor (TFPI) in the HEPTEST assay and in an amidolytic anti factor Xa assay for LMW heparin.

    abstract：:Both the HEPTEST and amidolytic anti factor Xa assays are currently being used for heparin activity detection in plasma from patients receiving standard heparin or low molecular weight heparin (LMWH). In this study we have investigated the influence of recombinant and endogenous Tissue Factor Pathway Inhibitor (TFPI) ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Kristensen HI,Ostergaard PB,Nordfang O,Abildgaard U,Lindahl AK

    更新日期：1992-09-07 00:00:00

  • Gelatinase B (MMP-9) deficiency does not affect murine adipose tissue development.

    abstract：:This study was performed to follow up on the observation that gelatinase A (MMP-2) deficiency impairs adipose tissue development in mice. The aim was to evaluate the role of its functional homologue gelatinase B (MMP-9) in adipose tissue growth. MMP-9 antigen levels were determined in lean and in obese women before an...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH09-10-0739

    authors： Van Hul M,Piccard H,Lijnen HR

    更新日期：2010-07-01 00:00:00

  • Obesity/insulin resistance rather than liver fat increases coagulation factor activities and expression in humans.

    abstract：:Increased liver fat may be caused by insulin resistance and adipose tissue inflammation or by the common I148M variant in PNPLA3 at rs738409, which lacks both of these features. We hypothesised that obesity/insulin resistance rather than liver fat increases circulating coagulation factor activities. We measured plasma...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH16-09-0716

    authors： Lallukka S,Luukkonen PK,Zhou Y,Isokuortti E,Leivonen M,Juuti A,Hakkarainen A,Orho-Melander M,Lundbom N,Olkkonen VM,Lassila R,Yki-Järvinen H

    更新日期：2017-01-26 00:00:00

  • Prevention of restenosis after percutaneous coronary interventions: the medical approach.

    abstract：:Restenosis following successful percutaneous coronary revascularization continues to represent a major problem limiting the clinical efficacy of this procedure. The underlying mechanisms of restenosis are comprised of a combination of effects from vessel recoil, negative vascular remodeling, thrombus formation and neo...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Rosanio S,Tocchi M,Patterson C,Runge MS

    更新日期：1999-09-01 00:00:00

  • Decreased platelet-free dopamine and unchanged noradrenaline and adrenaline in essential hypertension.

    abstract：:The content of free-catecholamines in blood platelets is much higher than in plasma and platelet catecholamines must be taken up from plasma, since platelets lack the enzymes for catecholamine synthesis. There is some evidence that platelet catecholamine content under certain circumstances may be an integrated measure...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Kjeldsen SE,Gjesdal K,Leren P,Eide IK

    更新日期：1988-10-31 00:00:00

  • A 23bp insertion in the endothelial protein C receptor (EPCR) gene impairs EPCR function.

    abstract：:EPCR is a type I transmembrane protein, highly expressed on the endothelium of large vessels, that binds protein C and augments its activation. In this study, a 23bp insertion in the EPCR gene was found in 4/198 survivors of myocardial infarction and 3/194 patients with deep vein thrombosis. The EPCR gene with the ins...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Biguzzi E,Merati G,Liaw PC,Bucciarelli P,Oganesyan N,Qu D,Gu JM,Fetiveau R,Esmon CT,Mannucci PM,Faioni EM

    更新日期：2001-10-01 00:00:00

  • Shear stress regulates inflammatory and thrombogenic gene transcripts in cultured human endothelial progenitor cells.

    abstract：:Shear stress has an established effect on mature endothelial cells, but less is known about how shear stress regulates endothelial progenitor cells (EPCs). In vitro expanded EPCs isolated from adult human blood represent a novel tool in regenerative vessel therapy. However, in vitro culturing may generate cells with u...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH09-12-0854

    authors： Lund T,Hermansen SE,Andreasen TV,Olsen JO,Østerud B,Myrmel T,Ytrehus K

    更新日期：2010-09-01 00:00:00

  • Do extra-platelet sources contribute to the plasma level of thrombospondin?

    abstract：:Thrombospondin, a trimeric glycoprotein contained in the platelet alpha-granules, has been proposed as a marker of in vivo platelet activation. However, it is also synthesised by a range of other cells. The extraplatelet contribution to plasma levels of thrombospondin was therefore estimated by investigating the relat...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Dawes J,Pratt DA,Dewar MS,Preston FE

    更新日期：1988-04-08 00:00:00

  • Endothelial cell protein S synthesis is upregulated by the complex of IL-6 and soluble IL-6 receptor.

    abstract：:We have recently demonstrated that the proinflammatory cytokine, interleukin-6 (IL-6), could upregulate the production of protein S in the human hepatoma cell line, HepG-2, but not in endothelial cells. In this study, we have demonstrated that the combination of exogenous IL-6 and soluble IL-6 receptor (sIL-6R) could ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Hooper WC,Phillips DJ,Evatt BL

    更新日期：1997-05-01 00:00:00

  • Bacterial expression of biologically active high molecular weight kininogen light chain.

    abstract：:Human high molecular weight kininogen (HK), a single chain plasma glycoprotein, serves as a cofactor in the contact system of blood coagulation. After cleavage by human plasma kallikrein, the nonapeptide bradykinin is released. The HK light chain (LC) contains coagulant activity, which requires both the ability to bin...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Kunapuli SP,DeLa Cadena RA,Colman RW

    更新日期：1992-04-02 00:00:00

  • D-dimer in local thrombolytic therapy with low doses of recombinant human tissue-type plasminogen activator (rt-PA) in patients with peripheral arterial occlusive disease.

    abstract：:The generation of D-dimer was studied in the course of local thrombolytic therapy of peripheral arterial occlusions with low doses of recombinant human tissue-type plasminogen activator (rt-PA) in 7 patients. Intermittent local application of rt-PA resulted in a marked increase in D-dimer exceeding values usually seen...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Koppensteiner R,Speiser W,Minar E,Ahmadi R,Ehringer H

    更新日期：1990-10-22 00:00:00

  • Genetic analysis in Japanese kindreds of congenital type I antithrombin deficiency causing thrombosis.

    abstract：:We have identified two novel minor deletions (case 1; -TA or -AT at nucleotide 9831-3 in exon 5 and case 2; -A at nucleotide 7640-1 in exon 4), one novel nonsense mutation (case 3; TAT to TAA at nucleotide 7491 in exon 4), and one recurrent nonsense mutation (case 4; CGA to TGA at nucleotide 5381 in exon 3A) in Japane...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Nakahara Y,Tsuji H,Nakagawa K,Masuda H,Kitamura H,Nishimura H,Kasahara T,Sugano T,Sawada S,Nakagawa M

    更新日期：1997-04-01 00:00:00

  • Intra-operative activation of coagulation--a stimulus to thrombosis mediated by vasopressin?

    abstract：:Vasopressin infusions in normal volunteers that produce concentrations in plasma comparable to those seen during stress, cause an increase in plasma factor VIII and shortening of the euglobulin clot lysis time (ECLT). We have investigated the relationship between endogenous vasopressin (aVP) release and haemostatic fu...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Grant PJ,Tate GM,Davies JA,Williams NS,Prentice CR

    更新日期：1986-02-28 00:00:00

  • Effect of staphylokinase-derived nonadecapeptide on the activation of plasminogen.

    abstract：:Staphylokinase (SAK) expresses plasminogen activator (PA) activity by forming a complex with plasmin. The interaction between the plasmin-SAK complex and plasminogen was investigated using synthesized peptides, which were constructed according to the amino acid sequence of the SAK molecule. A synthetic nonadecapeptide...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Okada K,Ueshima S,Matsuno H,Kawao N,Okamoto C,Tanaka M,Matsuo O

    更新日期：2007-05-01 00:00:00

  • Magnetic resonance imaging of tumor vasculature.

    abstract：:Angiogenic activity and formation of a vascular network facilitate tumor perfusion and play a critical role in tumor growth and metastasis. Tumor vasculature may be visualized by means of parametric imaging of specific morphological and physiological characteristics that collectively describe its properties. In this r...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Degani H,Chetrit-Dadiani M,Bogin L,Furman-Haran E

    更新日期：2003-01-01 00:00:00

  • Adhesion molecules and cardiovascular risk in peripheral arterial disease. Soluble vascular cell adhesion molecule-1 improves risk stratification.

    abstract：:Although intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) play a relevant role in atherosclerosis, little is known about the prognostic impact of their soluble forms (s) in patients with peripheral arterial disease (PAD). The aim of this prospective study was to verify whether ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH04-07-0440

    authors： Silvestro A,Brevetti G,Schiano V,Scopacasa F,Chiariello M

    更新日期：2005-03-01 00:00:00

  • Native atherosclerosis and vein graft arterialization: association with increased urokinase receptor expression in vitro and in vivo.

    abstract：:Interaction of proteases with cell surface receptors may modulate cell adhesion, migration, invasion, and matrix degradation. Since the plasminogen activator system has been hypothesized to play a role in intimal thickening after various types of vascular injury, we first studied the expression of urokinase receptor (...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Okada SS,Golden MA,Raghunath PN,Tomaszewski JE,David ML,Kuo A,Kariko K,Barnathan ES

    更新日期：1998-07-01 00:00:00

  • Heparin-induced thrombocytopenia (HIT): clinical and economic outcomes.

    abstract：:Heparin-induced thrombocytopenia (HIT) is an immune-mediated adverse drug reaction that occurs following exposure to unfractionated heparin (UFH) or low-molecular-weight heparin (LMWH). HIT with thrombosis (HITT) can cause devastating venous thromboembolism or arterial clots, prolonged hospitalization, and increased c...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Baroletti S,Piovella C,Fanikos J,Labreche M,Lin J,Goldhaber SZ

    更新日期：2008-12-01 00:00:00

  • Prothrombin Scranton: substitution of an amino acid residue involved in the binding of Na+ (LYS-556 to THR) leads to dysprothrombinemia.

    abstract：:Several members of a family from Scranton, Pennsylvania were identified to have normal levels of prothrombin antigen while their prothrombin clotting activity was approximately 50% of normal. There has been no previous history of bleeding or other clinical manifestations in this family. The genomic DNA from the proban...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Sun WY,Smirnow D,Jenkins ML,Degen SJ

    更新日期：2001-04-01 00:00:00

  • Human urinary soluble thrombomodulin (MR-33) improves disseminated intravascular coagulation without affecting bleeding time in rats: comparison with low molecular weight heparin.

    abstract：:We compared the antithrombotic and hemorrhagic effects of naturally existing human urinary soluble thrombomodulin (MR-33) with those of low molecular weight heparin (LMW-heparin) in rats. In in vitro experiments, MR-33 prolonged APTT in a dose-dependent fashion; its effect in this respect was as potent as that of LMW-...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Takahashi Y,Hosaka Y,Imada K,Adachi T,Niina H,Watanabe M,Mochizuki H

    更新日期：1997-04-01 00:00:00

  • Trend to efficacy and safety using antithrombin concentrate in prevention of thrombosis in children receiving l-asparaginase for acute lymphoblastic leukemia. Results of the PAARKA study.

    abstract：:An association has been reported between thrombotic events and the use of L-asparaginase (ASP) in children with acute lymphoblastic leukaemia (ALL). The mechanism for thrombosis is likely related to an acquired antithrombin deficiency. Since a primary prophylaxis using antithrombin concentrates may prevent thrombosis,...

    journal_title：Thrombosis and haemostasis

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1160/TH02-11-0283

    authors： Mitchell L,Andrew M,Hanna K,Abshire T,Halton J,Wu J,Anderson R,Cherrick I,Desai S,Mahoney D,McCusker P,Chait P,Abdolell M,de Veber G,Mikulis D

    更新日期：2003-08-01 00:00:00

  • Developmental haemostasis. Impact for clinical haemostasis laboratories.

    abstract：:Developmental haemostasis is a concept, now universally accepted, introduced by Andrew et al. in the late 1980's. However, coagulation analysers and reagents have changed significantly over the past 15 years. Coagulation testing is known to be sensitive to changes in individual reagents and analysers. We hypothesised ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH05-01-0047

    authors： Monagle P,Barnes C,Ignjatovic V,Furmedge J,Newall F,Chan A,De Rosa L,Hamilton S,Ragg P,Robinson S,Auldist A,Crock C,Roy N,Rowlands S

    更新日期：2006-02-01 00:00:00

  • Impaired fibrinolysis in obstructive jaundice--evidence from clinical and experimental studies.

    abstract：:Microvascular thrombosis is considered an important pathogenetic factor in renal failure associated with obstructive jaundice but the mechanisms leading to fibrin deposition are still unknown. The plasma levels of plasminogen activator inhibitor (PAI) in 29 patients with obstructive jaundice were found significantly i...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Colucci M,Altomare DF,Chetta G,Triggiani R,Cavallo LG,Semeraro N

    更新日期：1988-08-30 00:00:00

  • Phosphatidylethanolamine induces specific conformational changes in the kininogens recognizable by antiphosphatidylethanolamine antibodies.

    abstract：:Autoantibodies to the zwitterionic phospholipid (PL), phosphatidylethanolamine (PE) have been described in patients with thrombotic diseases. Recently, we reported that many antiphosphatidylethanolamine antibodies (aPE) are not specific for PE per se, but are directed to plasma proteins which can bind PE, for example,...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Sugi T,Mclntyre JA

    更新日期：1996-09-01 00:00:00

  • Potent inhibition of angiogenesis by D,L-peptides derived from vascular endothelial growth factor receptor 2.

    abstract：:Vascular endothelial growth factor (VEGF) is a potent mitogen for endothelial cells and plays a central role in angiogenesis and vasculogenesis. Therefore, VEGF and its receptors VEGFR-1 and VEGFR-2 are prime targets for anti-angiogenic intervention which is thought to be one of the most promising approaches in cancer...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH03-02-0106

    authors： Piossek C,Thierauch KH,Schneider-Mergener J,Volkmer-Engert R,Bachmann MF,Korff T,Augustin HG,Germeroth L

    更新日期：2003-09-01 00:00:00

  • The basement membrane underlying the vascular endothelium is not thrombogenic: in vivo and in vitro studies with rabbit and human tissue.

    abstract：:Studies examining the interaction of platelets with exposed subendothelium in vivo have reported conflicting results. To examine possible explanations for the apparently discrepant findings, we measured the platelet reactivity of subendothelium prepared by a number of methods both in vivo and in vitro. In addition, we...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Buchanan MR,Richardson M,Haas TA,Hirsh J,Madri JA

    更新日期：1987-08-04 00:00:00

  • Plasma levels of bradykinin are suppressed in factor XII-deficient mice.

    abstract：:A genetically-transmissible factor (F) XII-inactivated allele has been produced in mice by targeted replacement of exons 3-8 of the FXII gene with the neomycin resistance gene. Interbreeding of these mice provided offspring homozygous for two inactivated FXII alleles (FXII(-/-)). Male and female FXII-deficient mice br...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH06-03-0128

    authors： Iwaki T,Castellino FJ

    更新日期：2006-06-01 00:00:00