Abstract:
:Ultraviolet-B radiation (UVR) of mouse skin promotes both local and systemic immune aberrations that are thought to be important in the pathogenesis of cutaneous malignancies. Acute, low-dose UVR regimens inhibit the induction of contact hypersensitivity (CH) in genetically susceptible mice by TNF-alpha-dependent mechanisms. In addition, these regimens also promote the development of tolerance when hapten is applied to the UVR-exposed site at the completion of the radiation treatment protocol. A third immune abnormality is also observed in mice exposed to acute, low-dose UVR. This abnormality, which develops within 48-72 hr of the completion of the UVR regimen, has been described among antigen-presenting cells within secondary lymphoid organs, including lymph nodes that do not drain the site of irradiation. Dendritic cells (DCs) from lymph nodes and spleens of mice exposed to UVR lack the capacity to induce CH if they are derivatized with hapten and injected intracutaneously into naive mice. The DC defect is related to the production of and systemic dissemination of interleukin-10 (IL-10) by keratinocytes within the epidermis of the UVR-exposed skin. We have now examined the nature of the functional aberration that exists among DCs within the secondary lymphoid organs of UVR-exposed mice by examining the capacity of DCs to express co-stimulatory molecules, and their ability to activate ovalbumin (OVA) -specific DO11.10 T-cell receptor transgenic T cells in vitro. Our results indicate that DCs from UVR-exposed mice produced insufficient amounts of IL-12. When pulsed with OVA, these cells were capable of inducing proliferation among DO11.10 T cells in vitro, but the responding cells produced neither IFN-gamma nor IL-10 and IL-4. A similar antigen-presenting cell defect was generated in mice treated with a subcutaneous injection of IL-10. We conclude that acute, low-dose UVR creates an IL-10-dependent functional deficit in DCs in secondary lymphoid organs, and that this defect robs UVR-exposed mice of the capacity to develop CH when hapten is painted epicutaneously.
journal_name
Immunologyjournal_title
Immunologyauthors
Kitazawa T,Streilein JWdoi
10.1046/j.1365-2567.2000.00951.xkeywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
296-304issue
2eissn
0019-2805issn
1365-2567pii
imm951journal_volume
99pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Enriched CD4+CD8-/CD4-CD8-, CD4-CD8+/CD4-CD8- and CD4-CD8- cell suspensions were prepared from normal peripheral blood by selective immunomagnetic depletion of monoclonal antibody-defined lymphocyte populations. Subsequent examination of these modified cell fractions by two-colour flow cytometry provided a means of de...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::There is much evidence to suggest that potentially immunogenic tumour cells can escape cytolytic immune destruction by loss of class I antigen expression. Many tumours are allele-specific class I negative and, in murine systems, reconstitution of class I expression by gene transfection leads to an increase in tumour i...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The mouse T-cell tumours E1-4 and WEHI-22 produce an immunoglobulin (TCT Ig) which suppresses the antibody response to T- dependent antigens in vitro while having no effect on responses to T-independent antigens. TCT Ig also augments IgG responses to both kinds of antigen. TCT Ig appears to be a new class of mouse Ig ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::CD4(+) FOXP3(+) regulatory T (Treg) cells constitute a heterogeneous and plastic T-cell lineage that plays a pivotal role in maintaining immune homeostasis and immune tolerance. However, the fate of human Treg cells after loss of FOXP3 expression and the epigenetic mechanisms contributing to such a phenotype switch re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12198
更新日期:2014-03-01 00:00:00
abstract::The results presented in this paper demonstrate that a mouse IgM monoclonal antibody (T-80) recognizes an antigen on cells of the T-lymphocyte lineage of sheep. However, this antibody does not identify all T cells, as 10-20% of thymocytes and some peripheral-blood T cells are negative. T-80- thymocytes reside in the m...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::We produced three murine monoclonal antibodies (mAb) directed against the human T-cell differentiation antigen CD2 (formerly T11) and analysed their epitope specificity by E-rosette inhibition, cross-blocking and proliferation-induction experiments. When added together, two mAb (both IgG1 kappa) were able to induce pr...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::Toll-like receptors (TLRs) are important pattern recognition molecules that activate the nuclear factor (NF)-kappaB pathway leading to the production of antimicrobial immune mediators. As keratinocytes represent the first barrier against exogenous pathogens in human skin, we investigated their complete functional TLR1...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02122.x
更新日期:2005-04-01 00:00:00
abstract::As the burden of infectious diseases becomes reduced in many countries, a remarkable increase in the incidence of allergies has occurred. The basis for the rise in atopic disorders as a correlate of the decline in infectious diseases has not been defined. In the present study, we tested experimentally whether prior sy...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00856.x
更新日期:1999-11-01 00:00:00
abstract::Natural infection by mouse hepatitis virus (MHV) can affect interpretation of immunological studies in mice. MHV, a collective term describing a group of corona viruses, is found in natural infections in over 70% of laboratory mouse populations in the U.S.A. and Canada. Natural outbreaks of MHV in our animal colony af...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::The numbers of murine hepatocytes and Kupffer cells with receptors for human polymeric (pIgA) or monomeric IgA (mIgA) were determined by rosette formation with immunobeads coated with human pIgA or mIgA. About 63% of hepatocytes were found to express receptors for pIgA. Receptors for mIgA were also found in a signific...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::We have studied the antibody response in vitro of spleen cells from C57BL/6 mice kept on a protein deficient (D) or a normal diet (N). Short or long term protein restriction initiated after weaning led to increased plaque forming cell (PFC) responses to sheep red blood cells (SRBC), TNP-ficoll and TNP lipopolysacchari...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Lipoprotein lipase (LPL) is the key enzyme in the metabolism of triglyceride-rich lipoproteins. The patterns of LPL mRNA expression and secretion of the enzyme have not yet been established in natural killer (NK) cells. We show in the present communication that CD3- CD16+ cells (NK cells) transcribe LPL mRNA, express ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Apoptosis of macrophages infected with pathogenic mycobacteria is an alternative host defence capable of removing the environment supporting bacterial growth. In this work the influence of virulence and bacterial load on apoptosis of alveolar macrophages during the initial phase of infection by Mycobacterium bovis was...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03062.x
更新日期:2009-09-01 00:00:00
abstract::The class distribution of 4193 monoclonal gammopathies (MG), detected over approximately 10 years in western France (WF), was compared to that established by a compilation of twenty-seven series from the literature. Several points emerged from this study: the incidence of the light chain MG (4.8%) was slightly lower t...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::Infection of nude mice with Mesocestoides corti results in spleen enlargement, with a decrease in percentage positive, but overall increase in Thy-1 positive cells. A low frequency of both parasite antigen-specific and alloantigen-reactive T-cell clones were isolated from the spleen, mesenteric lymph nodes, peritoneal...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Mice were primed with TNP-derivatized insulin, or TNP-Mycobacteria, and lymph node cells were challenged in vitro with haptenated and unhaptenated antigens. Using either priming antigen, T-cell proliferative responses could be obtained to TNP-insulin. In B10 (H-2b), mice, which are responders to beef insulin (BI), but...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Rabbit spleen and mesenteric lymph node cells were treated with a monoclonal anti-rabbit T-lymphocyte antibody (MAb) and complement and the effect of the treatment on various lymphocyte functions was determined. Lysis of spleen and mesenteric lymph node cells reactive with this MAb, 9AE10, essentially eliminated their...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Fifteen sera of patients with visceral leishmaniasis were investigated for the occurrence of autoantibodies. They were found in high incidence and titre, and with specificity to the intermediate filament (INFIL) proteins vimentin (12 out of 15 with a titre higher than 1:10) and keratin (9 out of 15 with a titre higher...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::The interaction of mouse intestinal mast cell proteinase (IMCP) with serine proteinase inhibitors (serpins) in blood was analysed: (i) by examining the capacity of the inhibitors in blood to block the binding of the irreversible serine esterase inhibitor [3H]diisopropyl fluorophosphate (DFP); (ii) by Western blotting....
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Macrophages have the potential to recognize apoptotic neutrophils and phagocytose them while the same function for monocytes is uncertain. In fact, early findings indicated that monocytes started to phagocytose neutrophils on the third day of differentiation to macrophages. Here we show, using flow cytometry and confo...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03087.x
更新日期:2009-09-01 00:00:00
abstract::Synthesis of interferon (IFN)-gamma by natural killer (NK) cells is an important pro-inflammatory event with interleukin (IL)-12 and IL-18 playing major inductive roles. However, other temporal events are likely to regulate such processes and as prostaglandin E2 (PGE2) is ubiquitous during inflammation this study test...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.01810.x
更新日期:2004-03-01 00:00:00
abstract::Purified pertussis toxin (PPT) is a potent mitogen for human T lymphocytes and is shown to cause rapid calcium mobilization in resting T cells, a T-cell line and CD3- lymphocytes with natural killer (NK) activity. In resting T cells the PPT activation is associated with cytoplasmic alkalinization. A similar rise in in...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::The effect of a proline-rich polypeptide from ovine colostrum on the humoral immune response in mice against sheep red blood cells (SRBC) was studied. It was shown that the polypeptide had a regulatory activity stimulating or suppressing the immune response. The proline-rich polypeptide also increased the permeability...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::Dendritic cells (DC) generated from MUTZ-3, an immortalized acute myeloid leukaemia-derived cell line, have potential application as a model for the study of human DC, and as a tool with which to stimulate immunotherapeutic responses to cancer. However, the relationship of MUTZ-3 DC to their non-transformed counterpar...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.03018.x
更新日期:2009-07-01 00:00:00
abstract::Different kinds of IgM antibodies were tested for their activity in antibody-dependent cellular cytotoxicity (ADCC): firstly an anti-benzylpenicilloyl (BPO) IgM antibody from immune rabbit serum purified by affinity, ion exchange, and molecular-sieving chromatography, secondly two monoclonal rat anti-BPO IgM antibodie...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::Two herpes simplex virus type 1 (HSV-1) antigens of apparent molecular weight 123,000 and 63,000 which are associated with the viral glycoprotein complex have been identified and purified using virus-specific monoclonal antibodies. Mice immunized with either glycoprotein showed marked resistance to challenge with viru...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::The current study sought to delineate the gene expression profile of the host response in the caecum and colon during acute infection with Clostridium difficile in a mouse model of infection, and to investigate the nature of the unfolded protein response in this process. The infected mice displayed a significant up-re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12122
更新日期:2013-09-01 00:00:00
abstract::The phospholipid platelet-activating factor 1-0-hexadecyl-2-acetyl-SN-glycero-3-phosphorylcholine (AGEPC) initiates platelet function by interacting specifically with 1399 +/- 498 (mean +/- SD) high-affinity membrane receptors per platelet. In studies designated to characterize the high affinity binding site, AGEPC-hu...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::The biochemical analysis of murine Fc gamma RI has been difficult because this receptor is co-expressed with other Fc receptors on the cell surface of myeloid cells and all precipitate together with immune complexes. To overcome this problem and to study cells which expressed Fc gamma RI and only this Fc receptor, sta...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Osteopontin (OPN) is a secreted phosphoprotein found in body fluids (e.g. plasma, urine, milk) and in mineralized tissues. Its expression is increased in many transformed cells and in normal cells exposed to various cytokines. When stimulated with the inflammatory mediators lipopolysaccharide and interferon-gamma, mou...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-691.x
更新日期:1996-08-01 00:00:00