当前位置： SCI文献检索 > EUROPEAN JOURNAL OF PHARMACOLOGY期刊下所有文献 > Serotonin-stimulated increase in cytosolic Ca(2+) in cultured rat heart endothelial cells.

Serotonin-stimulated increase in cytosolic Ca(2+) in cultured rat heart endothelial cells.

Abstract：

:This study was designed to investigate the effects of serotonin on changes in intracellular Ca(2+) concentration ([Ca(2+)](i)) in cultured rat heart endothelial cells. Serotonin stimulated a biphasic change in cytosolic Ca(2+) of rat heart endothelial cells: an initial transient increase, which primarily reflects the release of Ca(2+) from internal stores, followed by a slow rise in [Ca(2+)](i) during the incubation with serotonin. Our study also demonstrated that the pattern of the serotonin-induced increase in [Ca(2+)](i) was different from that induced by thrombin in rat heart endothelial cells. In this study, the role of [Ca(2+)](i) on endothelial paracellular barrier function was also investigated. Serotonin induced an increase in endothelial permeability which paralleled the rise in [Ca(2+)](i) and was blocked by the 5-HT(2) receptor antagonist cyproheptadine. Therefore, the serotonin-stimulated increase in cytosolic Ca(2+) and macromolecular permeability was receptor-mediated in rat heart endothelial cells. Further experiments demonstrated that the serotonin-induced increase in [Ca(2+)](i) was inhibited by the phospholipase C inhibitors, neomycin and [6-[[17beta-3-methoxyestra-1,3, 5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U73122). Experiments involving the rapid depletion of intracellular Ca(2+) stores and Ca(2+)-free medium demonstrated that the biphasic response of endothelial Ca(2+) to serotonin was related to the release of Ca(2+) from intracellular stores and to the influx of extracellular Ca(2+). We also suggest that serotonin-induced changes in [Ca(2+)](i) are related to Ca(2+) channels sensitive to voltage-operated and inorganic Ca(2+) channel blockers.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Lee HZ,Wu CH

doi

10.1016/s0014-2999(99)00672-x

keywords:

subject

Has Abstract

pub_date

1999-11-12 00:00:00

pages

53-60

issue

1

eissn

0014-2999

issn

1879-0712

pii

S0014-2999(99)00672-X

journal_volume

384

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF PHARMACOLOGY文献大全
  • Role of tachykinin NK3 receptors in the release and effects of nerve growth factor in human isolated bronchi.

    abstract：:The nerve growth factor (NGF) is a neurotrophic factor essential for the development and survival of neurons. It has also been identified as a mediator of inflammation and can cause airway hyperresponsiveness [Frossard et al., Eur. J. Pharmacol. 500, 453 (2004)]. Evidence in rodents suggests a link between tachykinins...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2006.10.068

    authors： Naline E,Höglund CO,Vincent F,Emonds-Alt X,Lagente V,Advenier C,Frossard N

    更新日期：2007-04-10 00:00:00

  • Influence of metformin on mitochondrial subproteome in the brain of apoE knockout mice.

    abstract：:Neurodegenerative diseases are the set of progressive, age-related brain disorders, characterized by an excessive accumulation of mutant proteins in the certain regions of the brain. Such changes, collectively identified as causal factors of neurodegeneration, all impact mitochondria, imminently leading to their dysfu...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.12.036

    authors： Suski M,Olszanecki R,Chmura Ł,Stachowicz A,Madej J,Okoń K,Adamek D,Korbut R

    更新日期：2016-02-05 00:00:00

  • Restoration of endothelium-dependent relaxation in both hypercholesterolemia and diabetes by chronic taurine.

    abstract：:We examined the effects of taurine on levels of low-density lipoprotein (LDL) cholesterol and glucose, and an endothelium-dependent relaxation in response to acetylcholine in cholesterol-fed or streptozotocin-induced diabetic mice. The acetylcholine-induced concentration-dependent relaxation was significantly attenuat...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(96)00094-5

    authors： Kamata K,Sugiura M,Kojima S,Kasuya Y

    更新日期：1996-05-06 00:00:00

  • Cytotoxic and pro-apoptotic activities of cynaropicrin, a sesquiterpene lactone, on the viability of leukocyte cancer cell lines.

    abstract：:Cynaropicrin, a sesquiterpene lactone from Saussurea lappa, has been reported to possess immunomodulatory effects on cytokine release, nitric oxide production and immunosuppressive effects. In this study, we have examined cytotoxic effect of cynaropicrin against several types of cell lines such as macrophages, eosinop...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2004.03.027

    authors： Cho JY,Kim AR,Jung JH,Chun T,Rhee MH,Yoo ES

    更新日期：2004-05-25 00:00:00

  • Cinnamaldehyde reduces IL-1beta-induced cyclooxygenase-2 activity in rat cerebral microvascular endothelial cells.

    abstract：:Cinnamaldehyde is a principle compound isolated from Guizhi-Tang, which is a famous traditional Chinese medical formula used to treat influenza, common cold and other pyretic conditions. The aim of the present study was to investigate the effects of cinnamaldehyde on expression and activity of cyclooxygenase (COX) and...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2006.03.002

    authors： Guo JY,Huo HR,Zhao BS,Liu HB,Li LF,Ma YY,Guo SY,Jiang TL

    更新日期：2006-05-10 00:00:00

  • Characterization of the intrinsic activity for a novel class of cannabinoid receptor ligands: Indole quinuclidine analogs.

    abstract：:Our laboratory recently reported that a group of novel indole quinuclidine analogs bind with nanomolar affinity to cannabinoid type-1 and type-2 receptors. This study characterized the intrinsic activity of these compounds by determining whether they exhibit agonist, antagonist, or inverse agonist activity at cannabin...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2014.05.007

    authors： Franks LN,Ford BM,Madadi NR,Penthala NR,Crooks PA,Prather PL

    更新日期：2014-08-15 00:00:00

  • Biological evaluation of novel Pt(II) and Pd(II) complexes with pyrazole-containing ligands.

    abstract：:The new platinum (II) and palladium (II) complexes (2-4) with ligands 5-(2-hydroxyphenyl)-1,3-dimethyl-4-(dimethoxy)phosphonyl-1H]-pyrazole (1a) and 5-(2-hydroxyphenyl)-1,3-dimethyl-4-methoxycarbonyl-1H]-2-pyrazole (1b) were screened in a search for novel anticancer agents. Thus, alkylating activity, cytotoxicity, abi...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2004.08.053

    authors： Budzisz E,Krajewska U,Rozalski M,Szulawska A,Czyz M,Nawrot B

    更新日期：2004-10-11 00:00:00

  • Combination of OK432 and human interferon-alpha for treating viral-induced diabetes mellitus in mice.

    abstract：:We investigated the therapeutic effects of OK432 (picibanil; CAS39325-1-4), an immunomodulator that is derived from the Su strain of Streptococcus pyogenes. This agent was administered alone or combined with human interferon-alpha in a murine model of insulin-dependent diabetes mellitus. Interferon-alpha inhibits vira...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(97)01476-3

    authors： Kanda T,Kogure S,Nara M,Tsukui S,Utsugi T,Tomono S,Kawazu S,Nagai R,Kobayashi I

    更新日期：1998-01-26 00:00:00

  • Possible mechanisms of cannabinoid-induced antinociception in the spinal cord.

    abstract：:Anandamide is an endogenous ligand at both the inhibitory cannabinoid CB(1) receptor and the excitatory vanilloid receptor 1 (VR1). The CB(1) receptor and vanilloid VR1 receptor are expressed in about 50% and 40% of dorsal root ganglion neurons, respectively. While all vanilloid VR1 receptor-expressing cells belong to...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/s0014-2999(01)01309-7

    authors： Morisset V,Ahluwalia J,Nagy I,Urban L

    更新日期：2001-10-19 00:00:00

  • Rapid desensitization and down-regulation of 5-HT2 receptors by DOM treatment.

    abstract：:The regulation of the 5-HT2 receptor-mediated head twitch response and of 5-HT2 receptor binding in the frontal cortex was studied in rats treated repeatedly with the 5-HT2 agonist 1-(2,5-dimethoxy-4-methylphenyl)-2-aminopropane (DOM) (2.5 mg/kg, s.c.). Four injections in 24 h produced a near maximal reduction in the ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90409-3

    authors： Leysen JE,Janssen PF,Niemegeers CJ

    更新日期：1989-04-12 00:00:00

  • Inhibitory effect of spinal mGlu(5) receptor antisense oligonucleotide on the up-regulated expression of spinal G protein associated with chronic morphine treatment.

    abstract：:Knockdown of spinal metabotropic glutamate 5 (mGlu5) receptor was shown to inhibit the development of intrathecal morphine antinociceptive tolerance. The present work was designed to evaluate the expression of spinal G-protein during morphine tolerance and knockdown of spinal mGlu5 receptor with antisense oligonucleot...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.11.024

    authors： Chen M,Zhang X,Xu H,Ma X,Jiang W,Xu T

    更新日期：2014-01-15 00:00:00

  • (+/-)-tamsulosin, an alpha 1A-adrenoceptor antagonist, inhibits the positive inotropic effect but not the accumulation of inositol phosphates in rabbit heart.

    abstract：:The influence of (+/-)-tamsulosin, a selective alpha 1A-adrenoceptor antagonist, on the positive inotropic effect and the accumulation of inositol phosphates that are induced via alpha 1-adrenoceptors was studied in comparison with that of another alpha 1A-adrenoceptor ligand oxymetazoline in the rabbit ventricular my...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(96)00442-6

    authors： Yang HT,Endoh M

    更新日期：1996-10-03 00:00:00

  • A novel anticancer agent Broussoflavonol B downregulates estrogen receptor (ER)-α36 expression and inhibits growth of ER-negative breast cancer MDA-MB-231 cells.

    abstract：:Estrogen receptor (ER)-negative breast cancers are aggressive and unresponsive to antiestrogens, and current therapeutic modalities for ER-negative breast cancer patients are usually associated with strong toxicity and side effects. Less toxic and more effective targeted therapies are urgently needed to treat this typ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.05.047

    authors： Guo M,Wang M,Deng H,Zhang X,Wang ZY

    更新日期：2013-08-15 00:00:00

  • Some effects of disopyramide and its N-dealkylated metabolite on isolated nerve and cardiac muscle.

    abstract：:In animals and man the antidysrhythmic agent disopyramide in primarily metabolised by mono-N-dealkylation. The effects of disopyramide and its N-dealkylated metabolite (MIP) have been investigated using isolated cardiac and nervous tissue, and their effects have been compared with the effects of other antidysrhythmic ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(78)90313-8

    authors： Grant AM,Marshall RJ,Ankier SI

    更新日期：1978-06-15 00:00:00

  • Characterization of histamine receptors in the ureter of the dog.

    abstract：:We investigated the effects of histamine on the motility of isolated segments from canine ureters and characterized pharmacologically the histamine receptors involved. We also evaluated the effects of various autacoids (5-HT, carbachol, noradrenaline, thromboxane, prostaglandin F2alpha) on the motility of canine urete...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(96)00765-0

    authors： Dodel RC,Hafner D,Borchard U

    更新日期：1996-12-30 00:00:00

  • Elements in support of the 'non-identity' of the PGRMC1 protein with the σ2 receptor.

    abstract：:σ2 Receptor subtype is overexpressed in a variety of human tumors, with σ2 agonists showing antiproliferative effects towards tumor cells through multiple pathways that depend both on the tumor cell type and on the molecule type. Therefore, σ2 receptor is an intriguing target for tumor diagnosis and treatment despite ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.03.067

    authors： Abate C,Niso M,Infantino V,Menga A,Berardi F

    更新日期：2015-07-05 00:00:00

  • Metabotropic receptor mediated afterdepolarization in neocortical neurons.

    abstract：:In rat neocortical slices activation of glutamate metabotropic receptors with glutamate, quisqualate and 1S,3R-ACPD caused a spike-evoked afterdepolarization which replaced the normal afterhyperpolarization. In some neocortical neurons the afterdepolarization reached action potential threshold causing prolonged self-s...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0922-4106(92)90073-5

    authors： Greene C,Schwindt P,Crill W

    更新日期：1992-07-01 00:00:00

  • Characterization of atypical beta-adrenoceptors in the guinea pig duodenum.

    abstract：:The atypical beta-adrenoceptors mediating relaxation in the guinea pig duodenum were studied using catecholamines (isoprenaline, noradrenaline and adrenaline), a selective beta3-adrenoceptor agonist BRL37344 ((R*,R*)-(+/-)-4-[2-[(2-(3-chlorophenyl)-2-hydroxyethyl)amino]propyl]phe noxyacetic acid sodium salt) and a non...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(99)00355-6

    authors： Horinouchi T,Koike K

    更新日期：1999-07-02 00:00:00

  • Inhibition of inducible nitric oxide synthase gene expression by glucocorticoid-induced protein(s) in lipopolysaccharide-stimulated J774 cells.

    abstract：:Glucocorticoids inhibit inducible-type NO synthase activity in a variety of cell types. We report here that proteins recovered from the medium of dexamethasone-treated J774 macrophages (1, 10, 100 microg/ml) inhibited lipopolysaccharide-stimulated nitrite generation by 10.0 +/- 3.0%, 32.3 +/- 5.3% and 55.0 +/- 6.0%, r...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(97)01361-7

    authors： D'Acquisto F,Cicatiello L,Iuvone T,Ialenti A,Ianaro A,Esumi H,Weisz A,Carnuccio R

    更新日期：1997-11-19 00:00:00

  • Pyrrolidine dithiocarbamate protects against scopolamine-induced cognitive impairment in rats.

    abstract：:Alzheimer's disease (AD) is a chronic neurodegenerative disorder that leads to disturbances of cognitive functions. Although the primary cause of AD remains unclear, brain acetylcholine deficiency, oxidative stress and neuroinflammation may be considered the principal pathogenic factors. The present study was construc...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.11.008

    authors： Abd-El-Fattah MA,Abdelakader NF,Zaki HF

    更新日期：2014-01-15 00:00:00

  • Nitric oxide mediates central activation of sympathetic outflow induced by interleukin-1 beta in rats.

    abstract：:The excitatory mechanism of central sympathetic outflow induced by interleukin-1 beta was investigated in urethane-anesthetized rats. Intracerebroventricular administration of interleukin-1 beta induced a gradually developing elevation of plasma noradrenaline levels in a dose-dependent manner (50, 100 and 200 ng/anima...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(96)00709-1

    authors： Murakami Y,Yokotani K,Okuma Y,Osumi Y

    更新日期：1996-12-12 00:00:00

  • Upregulation of NMDA receptor subunit proteins in the cerebral cortex during diazepam withdrawal.

    abstract：:The present study investigated the changes in NMDA receptor subunit proteins in diazepam-withdrawn rat cerebral cortex, using Western blotting analysis. The protein levels of the NR1 and NR2B, but not NR2A, subunits were significantly increased in diazepam-withdrawn rats compared to those in control rats. Therefore, a...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(97)01501-x

    authors： Tsuda M,Chiba Y,Suzuki T,Misawa M

    更新日期：1998-01-12 00:00:00

  • The role of cyclic AMP in the inhibition of leukotriene biosynthesis by neuropeptides.

    abstract：:Certain neuropeptides, including vasoactive intestinal peptide, inhibit peptidoleukotriene release from platelet activating factor-stimulated rat lung. We have now shown that vasoactive intestinal peptide will also inhibit peptidoleukotriene release from platelet activating factor-stimulated or ovalbumin-challenged gu...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90610-9

    authors： Di Marzo V,Tippins JR,Morris HR

    更新日期：1989-03-14 00:00:00

  • In vivo quantification of dopamine D2 receptor parameters in nonhuman primates with [123I]iodobenzofuran and single photon emission computerized tomography.

    abstract：:[123I]Iodobenzofuran ([123I]IBF) is a new single photon emission computed tomography (SPECT) tracer for visualization of the dopamine D2 receptors. A tracer constant infusion paradigm was developed to measure the binding potential, density (Bmax) and affinity (KD) of the dopamine D2 receptor in baboons. Three baboons ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)90521-5

    authors： Laruelle M,al-Tikriti MS,Zea-Ponce Y,Zoghbi SS,Baldwin RM,Charney DS,Hoffer PB,Kung HF,Innis RB

    更新日期：1994-09-22 00:00:00

  • Potentiation of formalin-evoked adenosine release by an adenosine kinase inhibitor and an adenosine deaminase inhibitor in the rat hind paw: a microdialysis study.

    abstract：:The present study examined the effects of local subcutaneous administration of formalin on adenosine release from the rat hind paw, and the effects of inhibitors of adenosine metabolism on such release. Microdialysis probes were inserted into the subcutaneous tissue of the plantar surface of rat hind paws. Samples wer...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(00)00742-1

    authors： Liu XJ,White TD,Sawynok J

    更新日期：2000-11-17 00:00:00

  • Chromodacryorrhea and repetitive hind paw tapping: models of peripheral and central tachykinin NK1 receptor activation in gerbils.

    abstract：:The in vivo pharmacological profiles of the selective tachykinin NK1 receptor agonists, [Sar9,Met(O2)11]substance P and GR 73632, were examined in gerbils. Both agonists induced a pronounced chromodacryorrhea following intravenous injection which was stereoselectively antagonised by the tachykinin NK1 receptor antagon...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)90198-8

    authors： Bristow LJ,Young L

    更新日期：1994-03-03 00:00:00

  • Initial assessment of single and repeat doses of inhaled umeclidinium in patients with chronic obstructive pulmonary disease: two randomised studies.

    abstract：:To characterise the safety, tolerability, pharmacodynamics (bronchodilatory effect) and pharmacokinetics of inhaled umeclidinium in patients with chronic obstructive pulmonary disease (COPD). The first investigation was a single dose, randomised, double-blind, placebo-controlled study (clinicaltrials.gov: NCT00515502)...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.ejphar.2012.12.019

    authors： Tal-Singer R,Cahn A,Mehta R,Preece A,Crater G,Kelleher D,Pouliquen IJ

    更新日期：2013-02-15 00:00:00

  • Anti-inflammatory effect of dual nociceptin and opioid receptor agonist, BU08070, in experimental colitis in mice.

    abstract：:Endogenous opioid and nociceptin systems are widely distributed in the gastrointestinal tract where they seem to play a crucial role in maintaining the intestinal homeostasis. The aim of our study was to assess whether activation of nociceptin (NOP) and µ-opioid (MOP) receptors by a mixed NOP/MOP receptor agonist, BU0...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.09.021

    authors： Zielińska M,Ben Haddou T,Cami-Kobeci G,Sałaga M,Jarmuż A,Padysz M,Kordek R,Spetea M,Husbands SM,Fichna J

    更新日期：2015-10-15 00:00:00

  • Characterization of histamine H1-receptors on astrocytes in primary culture: [3H]mepyramine binding studies.

    abstract：:The characteristics of histamine H1-receptors on astrocytes from the cerebral cortex of newborn rats in primary culture were analyzed with a [3H]mepyramine binding assay, and compared with those in the cerebral cortex. The apparent dissociation constant (KD) of [3H]mepyramine binding, the apparent inhibition constants...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90007-1

    authors： Inagaki N,Fukui H,Taguchi Y,Wang NP,Yamatodani A,Wada H

    更新日期：1989-11-28 00:00:00

  • Persistent release of noradrenaline caused by anticancer drug 4'-epidoxorubicin in rat tail artery in vitro.

    abstract：:Anthracycline derivatives including 4'-epidoxorubicin are known to cause cardiovascular side effects. In this study we examined the effects of 4'-epidoxorubicin on sympathetic nerves of rat tail artery in vitro. Treatment with 4'-epidoxorubicin at concentrations higher than 10 microM gradually increased the resting te...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00505-6

    authors： Sakai T,Inagaki R,Taniguchi T,Shinozuka K,Kunitomo M,Hayashi N,Ishii Y,Muramatsu I

    更新日期：1998-08-28 00:00:00