Abstract：
:A genetically modified recombinant tumor necrosis factor (TNF)-alpha (rKRKTNF) was conjugated to the terminal carboxyl groups of liposome grafted polyethyleneglycol (PEG) chains. The long-circulating liposomes were composed of egg phosphatidylcholine, cholesterol (chol) and 7% carboxyl PEG-phosphatidylethanolamine. The conjugation efficiency of the genetically modified rKRKTNF under the conditions described in the text was approximately 55%. The biological activity of liposomal rKRKTNF, as tested with an in vitro cytotoxicity assay was reduced compared to the free, unconjugated rKRKTNF. In vivo biodistribution studies showed that conjugation of as little as 0. 13% of the grafted PEG chains resulted in a rapid elimination of the formulation from the blood stream. It is speculated that both non-selective conjugate chemistry and inherent recognition of the TNF by the components of the reticuloendothelial system (RES) are responsible for the short blood half life of the rKRKTNF-PEG-liposomes. The result suggest that conjugating a rapidly clearing recombinant cytokine to long-circulating liposomes provides little advantage in modifying the pharmacokinetic parameters of the cytokine.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Savva M,Duda E,Huang Ldoi
10.1016/s0378-5173(99)00092-7keywords:
subject
Has Abstractpub_date
1999-07-05 00:00:00pages
45-51issue
1eissn
0378-5173issn
1873-3476pii
S0378-5173(99)00092-7journal_volume
184pub_type
杂志文章abstract：:Since premix membrane emulsification was developed as alternative technique for the preparation of emulsions and solid lipid particles as carrier systems for lipophilic drugs, many types of membranes have been used in this preparation process. The purpose of this study was to evaluate the influence of different types ...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2006-12-01 00:00:00
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2019-03-25 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2015.01.008
更新日期：2015-03-01 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2019.06.005
更新日期：2019-07-20 00:00:00
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pub_type： 杂志文章
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更新日期：2020-11-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2007.12.008
更新日期：2008-05-01 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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更新日期：2020-08-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2018.04.035
更新日期：2018-06-10 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2012.05.064
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：2021-01-05 00:00:00
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更新日期：2019-07-20 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2012.04.080
更新日期：2012-08-20 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2009.05.017
更新日期：2009-07-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2018-01-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2006-03-27 00:00:00
abstract：BACKGROUND:Perfluorocarbon-nanoemulsions (PFC-NE) made of PFC and phospholipids (PL) by homogenization are optimal for in vivo-19F labelling of monocytes and subsequently of inflamed tissues in magnetic resonance imaging (MRI). Necessary requirements for in vivo use of PFC-NE are sterility, suitable droplet sizes and t...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2019.118753
更新日期：2019-12-15 00:00:00
abstract：:Experiments were conducted to illustrate some of the challenges associated with measuring dynamic MDI aerosols. Experimental HFA-134a solution MDIs containing 8 or 20% ethanol were measured using the Andersen cascade impactor using three different inlets. It was demonstrated that the size distribution of MDI aerosols ...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2007.11.047
更新日期：2008-05-01 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2019.03.010
更新日期：2019-04-20 00:00:00
