Abstract：
:Photolysis or "uncaging" of caged compounds represents a significant tool in cell biology and chemistry. It provides a means for quantitative control of compound delivery with temporal and spatial resolution while observing their consequences for cellular signaling. We discuss the use of ultraviolet-transmitting optical fibers to directly deliver UV energy to the sample, combined with a nitrogen pulsed laser as a source of UV light. In this approach the size of the photolysis area is regulated by the exit aperture of the fiber tip which is controlled by pulling the optical fibers to desirable diameters. A diode (red) laser that is also coupled to the optical fiber aids the location of UV energy delivery through the fiber. We used this method to quantitatively uncage norepinephrine and calcium. The major advantage of this photolysis approach is its independence of microscope objectives and traditional optical pathways. Because the optical pathway of the microscope needs no modification to accommodate this photolysis system, integration with other experimental methods, such as electrochemistry, electrophysiology, confocal microscopy, and wide-field epifluorescence microscopy, is relatively simple.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Parpura V,Haydon PGkeywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
340-5issue
3eissn
0353-9504issn
1332-8166journal_volume
40pub_type
杂志文章abstract：AIM:To assess clinical and demographic characteristics of Turkish children and adolescents with obsessive-compulsive disorder. METHOD:The study comprised 47 children and adolescents (31 boys and 15 girls) aged between 9 and 15 years, who were diagnosed with obsessive-compulsive disorder according to Diagnostic and Sta...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：AIM:To examine the usefulness of molecular analysis of IgH gene rearrangement in assessment of clonality in bone marrow biopsies with lymphoid aggregates (LA) and/or nodular lymphoid hyperplasia (NLH) in patients with different subtypes of malignant lymphomas. METHOD:Five hundred and twenty nine samples of bone marrow...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：AIM:To ascertain health priorities of older patients and treatment priorities of their general practitioners (GP) on the basis of a geriatric assessment and to determine the agreement between these priorities. METHODS:The study included a sample of 9 general practitioners in Hannover, Germany, and a stratified sample ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2010.51.483
更新日期：2010-12-01 00:00:00
abstract：AIM:To assess genetic diversity and genetic distances among isolated populations from Dagestan. METHODS:A cross-population genetic epidemiology design was applied in ethnically and demographically diverse isolates from Dagestan, some with more than 200 and some with less than 100 generations of demographical history s...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2006-08-01 00:00:00
abstract：AIM:To report on our experience with a voice-directed robotic arm for scope management in different procedures for "solo-surgery" and in complex laparoscopic operations. METHODS:A chip card with orders for the robotic arm is individually manufactured for every user. A surgeon gives order through a microphone and the o...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Brain death or neurologic death has gradually become recognized as human death over the past decades worldwide. Nevertheless, in Japan, the New York State, and the State of New Jersey, death is declared based not on brain death criteria, but on cardio-pulmonary criteria. In Japan, the 1997 Organ Transplant Law legaliz...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2013.54.75
更新日期：2013-02-01 00:00:00
abstract：AIM:To estimate the incidence of postural orthostatic tachycardia syndrome (POTS) in the population of Zagreb, Croatia, and to determine the patients' demographic and clinical characteristics. METHODS:From 2012-2017, we identified patients with POTS by a retrospective analysis of medical records at University Hospital...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2020-10-31 00:00:00
abstract：AIM:To evaluate the results of current reforms in Macedonian health sector. METHOD:Description and situation analysis, covering the period 1991-1997, are focused on demographic and vital indicators, morbidity and mortality data, elements of health care system, legislation, health insurance, health care financing, and ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：AIM:To compare the prevalence and characteristics of bullying between two towns in Bosnia and Herzegovina-Stolac, which was exposed to firearm conflict during the 1992-1995 war in Bosnia and Herzegovina, and Posusje, which was outside of the active combat zone. METHODS:In this cross-sectional study, we included 484 pr...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2008.4.528
更新日期：2008-08-01 00:00:00
abstract：:Two closely related boys from the same house hold (Home 1), aged two and three, were affected with fulminant meningococcal sepsis known as Waterhouse-Friderichsen syndrome. Neisseria meningitidis serogorup B was isolated from their blood and cerebrospinal fluid. The two-year-old boy died one day after the onset of the...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：AIM:To analyze the haplotype of the Ezhava population of Kerala, south India, using 8 short tandem repeat (STR) loci on the Y chromosome and trace the paternal genetic lineage of the population. METHODS:Whole blood samples (n=104) were collected from unrelated healthy men of the Ezhava population over a period of one ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2011.52.344
更新日期：2011-06-01 00:00:00
abstract：:There may be valuable research going on in the developing and financially less-privileged countries, but it usually does not reach international visibility, in spite of a large number of scientific journals in these countries. Such journals are not only invisible but, by perpetuating a vicious circle of inadequacy, ma...
journal_title：Croatian medical journal
pub_type： 评论,社论
doi：
更新日期：2001-04-01 00:00:00
abstract：:A basic premise of this article is that the political and ethnic division of the city of Mostar imperils community health planning efforts beyond those normally encountered in the aftermath of war. Bosnia and Herzegovina is divided among ethnic groups, and the city of Mostar is further subdivided between the Croatian ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Throughout the Middle Ages a unique Croatian Glagolitic script co-existed with Latin and western Cyrillic scripts, thus creating an open, large, and tolerant cultural environment. Many works common to European cultural heritage were translated into Croatian (Church Slavonic) language and preserved in the Glagolitic sc...
journal_title：Croatian medical journal
pub_type： 历史文章,杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：AIM:To estimate the impact of high fat diet and estrogen deficiency on the oxidative and antioxidative status in the liver of the ovariectomized rats, as well as the ameliorating effect of physical activity or consumption of functional food containing bioactive compounds with antioxidative properties on oxidative damag...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2014.55.218
更新日期：2014-06-01 00:00:00
abstract：AIM:To assess the quality of diabetes care provided by family medicine teams in primary health centers in Bosnia and Herzegovina (BH) through a medical audit, addressing the extent to which clinical practice complied with pre-determined explicit criteria of long-term management. METHOD:Retrospective analysis included ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2008.49.757
更新日期：2008-12-01 00:00:00
abstract：AIM:To assess the diagnostic value of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in lung cancer (LC). We compared the ratios between healthy participants and all LC patients, as well patients with different pathohistological LC subtypes. METHODS:We retrieved the data on neutrophil, lym...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.287
更新日期：2016-06-30 00:00:00
abstract：AIM:Oncostatin-M, a member of the gp 130 family of cytokines, has been associated with inflammation, connective tissue production, and extracellular matrix turnover. Since the reperfused heart is associated with an intense inflammatory reaction followed by scar formation, we tested the hypothesis that oncostatin-M is u...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：AIM:To determine the incidence of possible transfusion-related acute lung injury (TRALI) and related risk factors in cardiac surgery patients. METHODS:A single-center prospective cohort study was conducted from January 2009 to March 2010 at the Zagreb University Hospital Center, Croatia. Patient-, transfusion-, and su...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2014.55.138
更新日期：2014-04-01 00:00:00
abstract：AIM:To investigate missed opportunities to reveal existing but not formerly diagnosed coronary heart disease cases and related risk factors in primary health care. METHODS:The study comprised 850 people aged over 30 years with no known history of coronary heart disease, receiving health services from a primary care ce...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：AIM:To evaluate the accuracy of eye color prediction based on six IrisPlex single nucleotide polymorphisms (SNP) in a Slovenian population sample. METHODS:Six IrisPlex predictor SNPs (HERC2 - rs12913832, OCA2 - rs1800407, SLC45A2 - rs16891982 and TYR - rs1393350, SLC24A4 - rs12896399, and IRF4 - rs12203592) of 105 ind...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2013.54.381
更新日期：2013-08-01 00:00:00
abstract：AIM:To assess the usefulness of a DNA-based method for identifying mushroom species for application in forensic laboratory practice. METHODS:Two hundred twenty-one samples of clinical forensic material (dried mushrooms, food remains, stomach contents, feces, etc) were analyzed. ITS2 region of nuclear ribosomal DNA (nr...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2015.56.32
更新日期：2015-02-01 00:00:00
abstract：:Hydrocephalus is a common brain disorder that is treated only with surgery. The basis for surgical treatment rests on the circulation theory. However, clinical and experimental data to substantiate circulation theory have remained inconclusive. In brain tissue and in the ventricles, we see that osmotic gradients drive...
journal_title：Croatian medical journal
pub_type： 杂志文章,评审
doi：10.3325/cmj.2014.55.366
更新日期：2014-08-28 00:00:00
abstract：AIM:To evaluate psychosomatic complaints and depressive symptoms among civilians, refugees, and soldiers in the war and post-war period in Croatia. METHOD:The design of the study was longitudinal, including four repeated assessments during the war and post-war period (1993, 1995, 2000, and 2004). The baseline assessme...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：: ...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2018-10-31 00:00:00
abstract：:Kidney transplantation is the most efficient method of renal replacement therapy. When this method is performed, native urinary bladder is the preferred urinary reservoir. However, in some patients with an anatomically and functionally abnormal lower urinary tract, the urinary bladder cannot be used for transplantatio...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2019-12-31 00:00:00
abstract：AIM:To compare the myocardial effects of cardioplegia by warm blood, tepid blood, and cold crystalloid during coronary artery bypass grafting (CABG). METHODS:Patients undergoing CABG surgery at Kaunas University Hospital between 2000 and 2004 were randomized into three groups (n=156), receiving a different method of c...
journal_title：Croatian medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2005-12-01 00:00:00
abstract：AIM:To test the hypothesis that tSSEP findings reflect clinical and MRI MS lesions, the aim of this study was to investigate tSSEP changes in patients with clinically isolated syndrome (CIS) in relation to clinical and brainstem MRI findings. The second aim was to investigate whether the interpretation of the tSSEP res...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2016.57.558
更新日期：2016-12-31 00:00:00
abstract：:A study was conducted to determine if modern forensic DNA typing methods can be properly employed throughout the world with a final goal of increasing arrests, prosecutions, and convictions of perpetrators of modern day trafficking in persons while concurrently reducing the burden of victim testimony in legal proceedi...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：10.3325/cmj.2014.55.3
更新日期：2014-02-01 00:00:00
abstract：AIM:To investigate the influence of 1991-1995 war on the prevalence, duration, and practice of breast-feeding Croatian children up to 5 years of age. METHOD:In 1996, interviews were conducted in households with children up to 2 years of age (757 children) and 2-5 years of age (1,180 children). Data for war-free areas,...
journal_title：Croatian medical journal
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
