Automated prozone effect detection in ferritin homogeneous immunoassays using neural network classifiers.

abstract：BACKGROUND:Chronic atrophic gastritis (CAG) is a precursor of the intestinal type of gastric cancer, the second leading cause of cancer-related death worldwide. GastroPanel is a recently marketed serological kit for the non-invasive diagnosis of CAG, defined by some authors "even more reliable than biopsy histology". T...

journal_title：Clinical chemistry and laboratory medicine

authors： Lombardo L,Leto R,Molinaro G,Migliardi M,Ravarino N,Rocca R,Torchio B

更新日期：2010-09-01 00:00:00

abstract：:The importance of proper consumption of dietary folate for human health has been highlighted by an extensive number of publications over several decades. Fortification of grain products with folic acid was initiated with the specific intent to prevent neural tube defects, and the scope of this endeavor is unique in th...

journal_title：Clinical chemistry and laboratory medicine

authors： Strickland KC,Krupenko NI,Krupenko SA

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:The aim was to investigate whether first trimester glycodelin and angiopoietin-2 can predict small-for-gestational age (SGA) at delivery, individually or in combination. METHODS:In this case-control study we measured glycodelin and angiopoietin-2 on serum from 170 singleton pregnant women delivering SGA neo...

journal_title：Clinical chemistry and laboratory medicine

authors： Hansen YB,Myrhøj V,Jørgensen FS,Sørensen S

更新日期：2016-09-01 00:00:00

abstract：:Intake of drugs may influence the interpretation of laboratory test results. Knowledge and correct interpretation of possible drug-laboratory test interactions (DLTIs) is important for physicians, pharmacists and laboratory specialists. Laboratory results may be affected by analytical or physiological effects of medic...

journal_title：Clinical chemistry and laboratory medicine

authors： van Balveren JA,Verboeket-van de Venne WPHG,Erdem-Eraslan L,de Graaf AJ,Loot AE,Musson REA,Oosterhuis WP,Schuijt MP,van der Sijs H,Verheul RJ,de Wolf HK,Kusters R,Hoedemakers RMJ,Dutch Society for Clinical Chemistry and Laborat

更新日期：2018-11-27 00:00:00

abstract：BACKGROUND:Our laboratory was confronted with two successive urine samples from a single patient which tested positive for human chorionic gonadotropin (hCG) when tested with both qualitative and quantitative assays, combined with no detectable hCG in corresponding plasma samples. METHODS:Serial dilution and recovery ...

journal_title：Clinical chemistry and laboratory medicine

authors： Albersen A,Kemper-Proper E,Thelen MH,Kianmanesh Rad NA,Hoedemaeker RF,Boesten LS

更新日期：2011-06-01 00:00:00

abstract：:Homocysteine is a risk factor for ischemic heart disease; similarly as is hyperlipidemia or insulin resistance, which frequently occur in women with polycystic ovary syndrome. We examined the relationships between thiols and hormonal status or insulin resistance in 40 women (aged 25.8 +/- 7 years) with polycystic ovar...

journal_title：Clinical chemistry and laboratory medicine

authors： Vrbíková J,Tallová J,Biciková M,Dvoráková K,Hill M,Stárka L

更新日期：2003-02-01 00:00:00

abstract：:Age-related macular degeneration (AMD) is a sight threatening eye disease that affects millions of humans over the age of 65 years. It is considered to be the major cause of irreversible blindness in the elderly population in the developed world. The disease is prevalent in Europe and the United States, which has a la...

journal_title：Clinical chemistry and laboratory medicine

authors： Kokotas H,Grigoriadou M,Petersen MB

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND:Automated systems for antinuclear antibody analysis are being introduced. The aim was to evaluate whether automated quantitative reading of fluorescence intensity is clinically relevant and allows for value-added reporting of test results. METHODS:Consecutive samples (n=260) were used to correlate fluoresce...

journal_title：Clinical chemistry and laboratory medicine

authors： Schouwers S,Bonnet M,Verschueren P,Westhovens R,Blockmans D,Mariën G,Bossuyt X

更新日期：2014-04-01 00:00:00

abstract：BACKGROUND:Detection of systemic sclerosis-associated autoantibodies (SSc-Ab) is mostly restricted to anti-centromere and anti-topoisomerase-I. However, anti-RNA-polymerase-III and anti-PM/Scl are also important diagnostic markers for the disease supporting their incorporation in the laboratory repertoire. The aim of t...

journal_title：Clinical chemistry and laboratory medicine

authors： Bonroy C,Smith V,Van Steendam K,Van Praet J,Deforce D,Devreese K,De Keyser F

更新日期：2013-11-01 00:00:00

abstract：Objectives:Colorectal cancer (CRC) screening using stool samples is now in routine use where tumor DNA methylation analysis for leading markers such as NDRG4 and SDC2 is an integral part of the test. However, processing stool samples for reproducible and efficient extraction of human genomic DNA remains a bottleneck fo...

journal_title：Clinical chemistry and laboratory medicine

authors： Jin S,Ye Q,Hong Y,Dai W,Zhang C,Liu W,Guo Y,Zhu D,Zhang Z,Chen S,Wang Y,Li D,Ma W,Yang Z,Li J,Zheng Z,Luan J,Wu X,Jiang F,Xu C,Ding C

更新日期：2020-07-16 00:00:00

abstract：:The measurement of glycated haemoglobin has become centrally important in the monitoring of glycaemic control in the patient with diabetes. A number of analytical techniques have been described to measure this important haemoglobin fraction, and the fraction measured depends on the technique used; this has resulted in...

journal_title：Clinical chemistry and laboratory medicine

authors： John WG

更新日期：2003-09-01 00:00:00

abstract：:Protein-coding genes comprise only 3% of the human genome, while the genes that are transcribed into RNAs but do not code for proteins occupy majority of the genome. Once considered as biological darker matter, non-coding RNAs are now being recognized as critical regulators in cancer genome. Among the many types of no...

journal_title：Clinical chemistry and laboratory medicine

authors： Ling H,Girnita L,Buda O,Calin GA

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:The handling of cerebrospinal fluid (CSF) affects the biomarker quantification used to diagnose Alzheimer's disease (AD). Only specialized centers can test for AD markers. The precise timing and freezing is required to correctly measure these biomarkers. Therefore, the effects of CSF storage temperature and ...

journal_title：Clinical chemistry and laboratory medicine

authors： Klener J,Hofbauerová K,Bartoš A,Ríčný J,Rípová D,Kopecký V

更新日期：2014-05-01 00:00:00

abstract：:Lipid peroxidation biomarkers and antioxidant status were measured in 76 cystic fibrosis (CF) patients and compared to 40 control subjects. Univariate and multivariate statistics were performed in this study. Results showed that indicators of lipid peroxidation were higher in CF patients than in controls; thiobarbitur...

journal_title：Clinical chemistry and laboratory medicine

authors： Benabdeslam H,Abidi H,Garcia I,Bellon G,Gilly R,Revol A

更新日期：1999-05-01 00:00:00

abstract：BACKGROUND:Celiac disease (CD) is an immune-mediated disorder of the small bowel in which gluten and related cereal ingestion leads to chronic inflammation and damage to the small bowel mucosa in genetically susceptible individuals. The presence of IgA type tissue-transglutaminase antibodies (IgA-anti-tTG) in individua...

journal_title：Clinical chemistry and laboratory medicine

authors： Ress K,Harro J,Uibo O,Uibo R

更新日期：2011-06-01 00:00:00

abstract：BACKGROUND:Paraoxonases (PON) are members of an enzyme family involved in preventing low-density lipoprotein oxidation and therefore protecting against atherosclerotic plaque formation. METHODS:We studied the Met55Leu and Gln192Arg PON1 polymorphisms in 712 patients (437 Caucasian- and 275 African-Brazilians) who unde...

journal_title：Clinical chemistry and laboratory medicine

authors： Rios DL,D'Onofrio LO,Cerqueira CC,Bonfim-Silva R,Carvalho HG,Santos-Filho A,Galvão-Castro B

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The Sysmex DI-60 system (DI-60, Sysmex, Kobe, Japan) is a new automated digital cell imaging analyzer. We explored the performance of DI-60 in comparison with Sysmex XN analyzer (XN, Sysmex) and manual count. METHODS:In a total of 276 samples (176 abnormal and 100 normal samples), white blood cell (WBC) dif...

journal_title：Clinical chemistry and laboratory medicine

authors： Kim HN,Hur M,Kim H,Kim SW,Moon HW,Yun YM

更新日期：2017-11-27 00:00:00

abstract：:Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired disorder of the pluripotent stem cell resulting from the somatic mutation of the X-linked PIG-A gene, involved in the synthesis of the glycosylphosphatidyl-inositol anchor of membrane proteins such as CD55, CD59 and CD14. In the past decade, flow cytometry has b...

journal_title：Clinical chemistry and laboratory medicine

authors： Hevessy Z,Nagy B Jr,Kiss F,Kiss A,Kappelmayer J

更新日期：2005-01-01 00:00:00

abstract：:Although affinity and kinetic measurements on their own provide useful information regarding the suitability of antibodies for various immunoassays, a thermodynamic analysis provices additional information that throws light on the molecular forces at work in the antigen-antibody interaction. It may then be possible to...

journal_title：Clinical chemistry and laboratory medicine

authors： Van Regenmortel MH

更新日期：1998-06-01 00:00:00

abstract：BACKGROUND:Efficient laboratory service is the cornerstone of modern health care systems. Scientific innovations have contributed to substantial improvements in the field of laboratory science, but errors still prevail. These errors are classified as preanalytical, analytical and postanalytical, depending upon the time...

journal_title：Clinical chemistry and laboratory medicine

authors： Goswami B,Singh B,Chawla R,Mallika V

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:The immunoglobulin superfamily receptor translocation-associated 2 (IRTA2) gene encodes both membrane and secreted forms of the B-cell differentiation antigen (also identified as FcRH5). The membrane form is highly expressed on the surface of hairy cell leukemia (HCL) cells from patients. This study aimed to...

journal_title：Clinical chemistry and laboratory medicine

authors： Ise T,Kreitman RJ,Pastan I,Nagata S

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:The measurement of serum IgE aids in the diagnosis and management of atopic allergic disease and hyper-IgE immunodeficiency syndromes. The 2nd World Health Organization (WHO) International Reference Reagent (IRR) for serum IgE (75/502; 5000 IU/ampoule), is widely used to calibrate assays for serum IgE. Exhau...

journal_title：Clinical chemistry and laboratory medicine

authors： Thorpe SJ,Heath A,Fox B,Patel D,Egner W

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:Extensive application of measurement of urinary iodine concentration (UIC) in several benign and malignant thyroid diseases could profit by the availability of rapid and inexpensive measuring techniques. Aim of this study was to apply a simple and inexpensive commercially available potentiometric method for ...

journal_title：Clinical chemistry and laboratory medicine

authors： Giovacchini G,Giovanella L,Haldemann A,Staub U,Füchsel FG,Koch P

更新日期：2015-10-01 00:00:00

abstract：:Steroid 21-hydroxylase deficiency is present in more than 90% of patients with congenital adrenal hyperplasia, an inherited metabolic disorder of adrenal steroidogenesis. Impaired enzymatic activity leads to the accumulation of metabolic intermediates (progesterone and 17-hydroxyprogesterone), which results in excessi...

journal_title：Clinical chemistry and laboratory medicine

authors： Concolino P,Mello E,Zuppi C,Capoluongo E

更新日期：2010-08-01 00:00:00

abstract：BACKGROUND:It has been proposed that only anti-thyroid-peroxidase (TPOAb) and not anti-thyroglobulin (TgAb) antibodies should be assayed in the diagnosis of autoimmune thyroid disease (AITD). METHODS:We analyzed the thyroid autoantibody profile with a multiplex bead array system, which allows simultaneous measurement ...

journal_title：Clinical chemistry and laboratory medicine

authors： Tozzoli R,Villalta D,Kodermaz G,Bagnasco M,Tonutti E,Bizzaro N

更新日期：2006-01-01 00:00:00

abstract：:Cytokines are key mediators in cell regulation and communication. The concentration of these proteins can rapidly and importantly increase during severe clinical situations. However, current techniques are not adapted to stat measurement, thus making their clinical use limited. In this context, the commercialization o...

journal_title：Clinical chemistry and laboratory medicine

authors： Berthier F,Lambert C,Genin C,Bienvenu J

更新日期：1999-05-01 00:00:00

abstract：:Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, is formed by methylation of arginine residues in proteins and released after proteolysis. In this reaction, S-adenosylmethionine is methyldonor and S-adenosylhomocysteine the demethylated product. ADMA and homocysteine are thus bioch...

journal_title：Clinical chemistry and laboratory medicine

authors： van Guldener C,Nanayakkara PW,Stehouwer CD

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Reference laboratories providing reference services recognized by the Joint Committee for Traceability in Laboratory Medicine (JCTLM) must be accredited as calibration laboratories according to ISO 17025 and ISO 15195. These standards require laboratories to establish an uncertainty budget, in which the unce...

journal_title：Clinical chemistry and laboratory medicine

authors： Rami L,Canalias F

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:EGFR mutation is an emerging biomarker for treatment selection in non-small-cell lung cancer (NSCLC) patients. However, optimal mutation detection is hindered by complications associated with the biopsy procedure, tumor heterogeneity and limited sensitivity of test methodology. In this study, we evaluated th...

journal_title：Clinical chemistry and laboratory medicine

authors： Shin S,Kim J,Kim Y,Cho SM,Lee KA

更新日期：2017-10-26 00:00:00

abstract：:This article provides an overview of the role of metabolite toxicity, low-grade inflammation and disturbed cellular signaling in obesity, glucose intolerance and diabetes. It also highlights links between this continuum of deteriorating glucose tolerance and atherosclerosis. Obesity, diabetes mellitus, and cardiovascu...

journal_title：Clinical chemistry and laboratory medicine

authors： Dominiczak MH

更新日期：2003-09-01 00:00:00