Abstract：
:Vascular endothelial (VE)-cadherin is the endothelium-specific member of the cadherin family of homotypic cell adhesion molecules. VE-cadherin, but not the cell adhesion molecule platelet/endothelial cell adhesion molecule (PECAM-1), markedly colocalizes with actin stress fibers at cell-cell junctions between human umbilical vein endothelial cells. Inhibition of VE-cadherin-mediated, but not PECAM-1-mediated, adhesion induced reorganization of the actin cytoskeleton, loss of junctional VE-cadherin staining and loss of cell-cell adhesion. In functional assays, inhibition of VE-cadherin caused increased monolayer permeability and enhanced neutrophil transendothelial migration. In a complementary set of experiments, modulation of the actin cytoskeleton was found to strongly affect VE-cadherin distribution. Brief stimulation of the beta2-adrenergic receptor with isoproterenol induced a loss of actin stress fibers resulting in a linear, rather than 'jagged', VE-cadherin distribution. The concomitant, isoproterenol-induced, reduction in monolayer permeability was alleviated by a VE-cadherin-blocking antibody. Finally, cytoskeletal reorganization resulting from the inactivation of p21Rho caused a diffuse localization of VE-cadherin, which was accompanied by reduced cell-cell adhesion. Together, these data show that monolayer permeability and neutrophil transendothelial migration are modulated by VE-cadherin-mediated cell-cell adhesion, which is in turn controlled by the dynamics of the actin cytoskeleton.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Hordijk PL,Anthony E,Mul FP,Rientsma R,Oomen LC,Roos Dkeywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
1915-23eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 12)pub_type
杂志文章abstract：:PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking beta1 integrin or Ilk, loss of P...
journal_title：Journal of cell science
pub_type： 杂志文章
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abstract：:Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of ...
journal_title：Journal of cell science
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更新日期：1997-04-01 00:00:00
abstract：:Intracellular signal transduction occurs through cascades of reactions involving dozens of proteins that transmit signals from the cell surface, through a crowded cellular environment filled with organelles and a filamentous cytoskeleton, to specific targets. Numerous signaling molecules are immobilized or transiently...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01122
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abstract：:Cutaneous wound healing is accelerated by exogenous mechanical forces and is impaired in TRPC6-knockout mice. Therefore, we designed experiments to determine how mechanical force and TRPC6 channels contribute to wound healing using HaCaT keratinocytes. HaCaT cells were pretreated with hyperforin, a major component of ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.147314
更新日期：2014-10-01 00:00:00
abstract：:Osteoclasts are giant multinucleated myeloid cells specialized for bone resorption, which is essential for the preservation of bone health throughout life. The activity of osteoclasts relies on the typical organization of osteoclast cytoskeleton components into a highly complex structure comprising actin, microtubules...
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pub_type： 杂志文章,评审
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更新日期：2020-07-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2000-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1997-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2005-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2017-04-01 00:00:00
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更新日期：2016-02-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1986-09-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2010-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.080911
更新日期：2011-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.008607
更新日期：2007-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.114231
更新日期：2013-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1989-07-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1983-07-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2002-01-01 00:00:00
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更新日期：2010-02-01 00:00:00
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更新日期：2007-09-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2014-05-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1992-09-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1993-09-01 00:00:00
