Abstract：
BACKGROUND:Glioma is the most common malignant tumor in the adult human brain and has one of the lowest patient survival rates. MicroRNAs (miRNAs) play important roles in the development of cancers, including glioma, and potentially have valuable therapeutic applications in glioma; however, their specific functions and mechanisms of action have yet to be fully defined. Here, we report that miR-129-5p directly targets DNA (cytosine-5)-methyltransferase 3A (DNMT3A) and functions as a tumor-suppressor in glioma. METHOD:We analyzed the expression profiles of miR-129-5p and DNMT3A in glioma-related databases. Quantitative reverse transcription-PCR was applied to detect the level of miR-129-5p in glioma specimens and cell lines. Western blotting was applied to detect the level of DNMT3A. We examined the effect of miR-129-5p on the cell cycle and proliferation of glioma cells using CCK-8 and EDU assays and flow cytometry. TargetScan software predicted DNMT3A to be a target of miR-129-5p, which we confirmed by means of luciferase reporter assays and rescue experiments. RESULT:miR-129-5p was expressed at low levels in glioma and negatively correlated with glioma grade. Over-expression of miR-129-5p in U87and LN229 cells inhibited proliferation and blocked the cell cycle in G1 Phase. DNMT3A is a direct target of miR-129-5p, and miR-129-5p affects glioma cell proliferation by targeting DNMT3A. CONCLUSION:Taken together, our results demonstrate that miR-129-5p plays a significant role in glioma suppression through inhibition of DNMT3A, which may provide a novel therapeutic strategy for treatment of glioma and other DNMT3A-driven cancers.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Gu X,Gong H,Shen L,Gu Qsubject
Has Abstractpub_date
2018-09-15 00:00:00pages
2834-2847issue
9issn
1943-8141journal_volume
10pub_type
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2018-01-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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更新日期：2017-10-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2016-02-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2016-09-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2015-08-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2019-08-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2020-02-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-07-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-11-15 00:00:00
abstract：:This study reports a case of a 4-year-old boy patient with abnormalities of muscle tone, movement and motor skills, as well as unstable gait leading to frequent falls. The results of the electroencephalogram (EEG) indicate moderately abnormal EEG, accompanied by irregular seizures. Based on these clinical characterist...
journal_title：American journal of translational research
pub_type：
doi：
更新日期：2018-03-15 00:00:00
abstract：:Krüppel-like factors (KLFs), such as KLF4, KLF2, KLF5 and KLF15, have been extensively investigated in multi-cancers. However, KLF16, a member of KLFs, hasn't been well identified in cancer, especially in gastric cancer (GC). Here, we investigated the roles of KLF16 in GC. In present study, we found that KLF16 express...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-06-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-08-15 00:00:00
abstract：:MicroRNA-302a-5p (miR-302a-5p) has been implicated in several cancers; however, its role in human non-small cell lung carcinoma (NSCLC) remains unknown. In this study, we showed that miR-302a-5p is downregulated in NSCLC tissues and cell lines. Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine assays showed that overe...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-07-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-04-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-03-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2020-07-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-12-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-06-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-08-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-02-15 00:00:00
abstract：:Depression is prevalence in patients with laryngeal cancer. In this study, we aim to investigate whether psychological intervention could reduce the depression of patients receiving cancer treatment. In this study, 258 patients with laryngeal cancer were assigned into two groups with or without psychological intervent...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2017-05-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-09-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-10-15 00:00:00
abstract：:Interleukin-1β (IL-1β) plays an important role in brain injury after focal ischemia, and bone marrow-derived mesenchymal stem cells (BMSCs) are capable of reducing the expression of IL-1β, we investigated the effects of BMSCs transplantation on brain edema and cerebral infarction as well as the underlying mechanisms v...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-03-15 00:00:00
