Abstract:
:This study aimed to explore the effects of long non-coding RNA (lncRNA) expression on rheumatoid arthritis (RA). LncRNA expression profiles were obtained from the synovial tissues of five RA patients and five age-/gender-matched controls by RNA-Seq. Six candidate lncRNAs were then chosen and their levels in synovial fluid further examined in 25 RA patients and 25 health controls using RT-qPCR. The effects of lncRNA RP11-83J16.1 overexpression and knockdown on RA fibroblast-like synoviocytes (RA-FLS) function, inflammation state, and URI1, FRAT1, and β-catenin levels were assessed. After RNA-Seq, lncRNA expression profiles clearly distinguished RA patients from controls, and 190 upregulated lncRNAs and 131 downregulated lncRNAs were identified, which were mainly enriched in proliferative/immune/inflammatory pathways. Results of RT-qPCR showed that the levels of lncRNAs MTCO2P12, KCNQ5-IT1 and RP11-83J16.1 were increased, whereas lncRNAs LINC00570, RP11-342M1.6, and REXO1L4P were decreased in RA patients compared to controls. Notably, lncRNA RP11-83J16.1 correlated with increased inflammation and disease activity in RA patients. Additionally, lncRNA RP11-83J16.1 promoted cell proliferation, migration, invasion and inflammation, reduced apoptosis, and positively regulates cellular URI1, FRAT1 and β-catenin expression in RA-FLS. Rescue experiments revealed that URI1 overexpression compensated for the regulatory effects of lncRNA RP11-83J16.1 knockdown in RA-FLS. In conclusion, lncRNA RP11-83J16.1, a novel lncRNA identified by RNA-Seq, correlates with increased risk and disease activity of RA, and promotes RA-FLS proliferation, migration, invasion and inflammation by regulating URI1 and downstream β-catenin pathway components.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Piao X,Zhou J,Hu Jsubject
Has Abstractpub_date
2020-04-15 00:00:00pages
1397-1414issue
4issn
1943-8141journal_volume
12pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
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abstract::Telocytes are novel cells that have been documented in the interstitium of multiple organs; however, their role in the heart remains unclear. This study aimed to identify cardiac telocytes by their morphological and molecular features and investigate whether their exosomes affect cardiac endothelial cells. To this end...
journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2014-10-11 00:00:00
abstract:BACKGROUND:Colorectal cancer is prevalent worldwide and it is also the fourth most common cause of cancer mortality. For rectal cancer, neoadjuvant concurrent chemoradiotherapy (CCRT) followed by radical proctectomy is gold standard treatment for patients with stage II/III rectal cancer. By data mining a public dataset...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
abstract::This study examined the homing capacity of human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) and their response to chemotactic gradients of stromal derived factor-1α (SDF). We have previously shown that EC derived from murine pluripotent stem cells can home to the ischemic hindlimb of the mouse....
journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2013-08-15 00:00:00
abstract::Macrophages are a major component of tumor stroma and the infiltrated macrophages in malignant tumor tissues (as called tumor associated macrophages, TAM) play a pivotal role in hepatocellular carcinoma (HCC) progression. However, the molecular mechanisms of macrophages promoting HCC metastasis are poorly understood. ...
journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2017-05-15 00:00:00
abstract::Acute myocardial ischemia/reperfusion (MIR) injury leads to severe arrhythmias and a high lethality. We aim to determine the effect of heat shock protein A12B (HSPA12B), a newly discovered member of the Hsp70 family, on heart injury parameters following MIR surgery. We used HSPA12B transgenic mice to determine its eff...
journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2015-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2020-06-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2019-02-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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更新日期:2017-04-15 00:00:00
abstract::RAB18, a member of the Ras family, has been suggested to play a critical role in multiple biological process. However, its functions in the development of hepatocellular carcinoma (HCC) remain unknown. In the present study, the expression and biological role of RAB18 in HCC were investigated. Results showed that the e...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::We tested the hypothesis that human induced pluripotent stem cell-derived mesenchymal stem cell (iPSC-MSC) therapy could effectively reduce brain-infarct volume (BIV) and improve neurological function in rat after acute intracranial hemorrhage (ICH) induced by a weight-drop device. Adult-male SD rats (n=40) were equal...
journal_title:American journal of translational research
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doi:
更新日期:2019-09-15 00:00:00
abstract::A surplus of food supply has evoked a worldwide increase in incidence of type 2 diabetes. This trend will have a significant impact on the life span of people living in modern societies. In contrast, reduced calorie intake has significant impact on preventing type 2 diabetes and increasing longevity. Increased product...
journal_title:American journal of translational research
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doi:
更新日期:2010-06-10 00:00:00
abstract::The prevention of doxorubicin (Dox) induced cardiotoxicity may be co-operative to recover future Dox treatment. The aim of this study was to explore the cardioprotective effects of oleanolic acid (OA), an antioxidant agent, on Dox induced cardiotoxicity. OA is a triterpenoid compound, which exist widely in plant kingd...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-01-15 00:00:00
abstract::The exact roles of copy number alteration (CNA) in initiation, progression and immunotherapy of breast cancer and the genomic alterations behind progression from ductal carcinoma in situ (DCIS) to invasive carcinoma remain unknown. Quantitative multi-gene fluorescence in situ hybridization (QM-FISH) opens a possibilit...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract::Principal component analysis (PCA) is a data analysis method that can deal with large volumes of data. Owing to the complexity and volume of the data generated by today's advanced technologies in genomics, pro-teomics, and metabolomics, PCA has become predominant in the medical sciences. Despite its popularity, PCA le...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2011-02-01 00:00:00
abstract::The goal of this study was to assess the role of high-mobility group box 1 (HMGB1)-induced endothelial cell (EC) pyroptosis in systemic inflammatory response syndrome (SIRS) following radiofrequency (RF) ablation of hepatic hemangiomas. We enrolled 76 patients with hepatic hemangioma who underwent RF ablation. Serum c...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00
abstract::An ideal implantation of artificial cervical disc replacement (ACDR) prosthesis should preserve the cervical biomechanics and maintain normal cervical biomechanics. In this study, we designed a novel prosthesis based on the physiological curvature of the cervical endplate, and conducted an in vitro test with cadaveric...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-06-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2010-01-01 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2018-12-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2018-05-15 00:00:00
abstract::NSUN5, a gene encodes a cytosine-5 RNA methyltransferase, is rarely mentioned in cancers. Our study is the first one to evaluate the role of NSUN5 in the progression of colorectal cancer. Data from TCGA was used to show the different expression of NSUN5 between CRC tumor tissues and adjacent normal ones. The NSUN5 exp...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2020-05-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2017-03-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2016-12-15 00:00:00