Abstract:
:Global microRNA (miRNA) profile may predict prostate cancer (PCa) behaviors. In this study, we examined global miRNA expression by miRNA profiling as well as specific miRNA expression levels in PCa epithelium and stroma by in situ hybridization (ISH) and correlated with various clinicopathological features. We first performed comprehensive miRNA profiling on 27 macrodissected cases of PCa by miRNA microarray. A total of 299 miRNAs were significantly dysregulated in high grade and advanced stage PCa. We demonstrated that PCa can be readily classified into high grade/stage and low-grade/stage groups by its global miRNA expression profile. Next, we examined the expression of several selected dysregulated miRNAs, including let-7c, miR-21, miR-27a, miR-30c, and miR-219, in PCa by ISH. The levels of miRNA expression in epithelial and stromal cells were scored semiquantitatively and compared with clinicopathological features, including age, race, Gleason score, stage, PSA recurrence, metastasis, hormone resistance and survival. We found that the expression of miR-30c and miR-219 were significantly down-regulated in PCa. miR-21 and miR-30c were significantly down-regulated in PCa in African Americans compared to Caucasian Americans. In addition, down-regulation of let-7c, miR-21, miR-30c, and miR-219 are associated with metastatic disease. Furthermore, down-regulation of miR-30c and let-7c are significantly associated with androgen-dependent PCa. In PCa stromal cells, let-7c downregulation is significantly associated with extraprostatic extension. Our data suggest that selected miRNAs may serve as potential biomarkers to predict cancer progression.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Ren Q,Liang J,Wei J,Basturk O,Wang J,Daniels G,Gellert LL,Li Y,Shen Y,Osman I,Zhao J,Melamed J,Lee Psubject
Has Abstractpub_date
2014-07-18 00:00:00pages
329-39issue
4issn
1943-8141journal_volume
6pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-12-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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更新日期:2017-11-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-06-15 00:00:00
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
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doi:
更新日期:2016-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-08-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2018-03-15 00:00:00
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journal_title:American journal of translational research
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doi:
更新日期:2016-10-15 00:00:00
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
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doi:
更新日期:2015-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-03-15 00:00:00
abstract::BikDD, a phosphorylation-mimic mutant of pro-apoptotic protein Bik, elicits strong apoptosis in cancer cells when introduced via an expression platform termed VP16-GAL4-WPRE integrated systemic amplifier (VISA) under the control of a cancer-specific promoter both in vitro and in vivo. C-VISA-BikDD expression plasmid e...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-02-15 00:00:00
abstract::Focal liver lesions (FLLs) include a variety of benign and malignant tumors, and are usually differential diagnosed by ultrasonography. However, traditional two-dimensional contrast-enhanced ultrasound (2D-CEUS) can evaluate a single slice of a lesion. It cannot fully display perfusion of a lesion and the relationship...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-05-15 00:00:00
abstract::Cyclooxygenase-2 expression by malignant tumors, including colonic adenocarcinoma, is associated with increased tumor aggression and poor prognosis. Nuclear factor kappa B is a key regulator of cyclooxygenase-2 and is regulated by two pathways, the 'canonical' and the 'alternative' pathway. The alternative pathway is ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00