Abstract：
BACKGROUND:The aim of this study was to explore the role of autophagy in the cold I/R injury following lung transplantation. METHODS:The rat orthotopic lung transplantation model was established to perform the level of autophagy in the cold I/R injury in this study. The pretreatment of inhibitor (3-Methyladenine [3-MA]) and activator (rapamycin [RAPA]) of autophagy were performed to assess the role of autophagy in the cold I/R injury following lung transplantation in rats. RESULTS:After lung transplantation, the autophagy, lung cell apoptosis and lung injury were aggravated and peaked at 6 h following the transplantation. The inhibition of autophagy by 3-MA induced downregulated of autophagy, decreased cell apoptosis. Meanwhile, the lung injury, which was indicated by calculating the peak inspiratory pressure (PIP), pulmonary vein blood gas analysis (PO2) and ratio of wet to dry in lung (W/D), was ameliorated after treatment with 3-MA. The activation of autophagy by RAPA causing the upregulated of autophagy and apoptosis of lung cells, and enhanced the lung injury. CONCLUSION:All the results suggested that the autophagy was involved in the cold I/R injury in lung transplantation model, and played a potential role on the regulation of I/R injury after lung transplantation.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Liu S,Zhang J,Yu B,Huang L,Dai B,Liu J,Tang Jsubject
Has Abstractpub_date
2016-08-15 00:00:00pages
3593-602issue
8issn
1943-8141journal_volume
8pub_type
杂志文章abstract：:Cholangiocarcinoma (CCA) is an aggressive tumour with a poor prognosis due to its late clinical presentation and the lack of effective non-surgical therapies. Previous studies have reported that platelets are implicated in tumour invasion and metastasis, while their role and the underlying mechanism in CCA remain uncl...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-07-15 00:00:00
abstract：:Babaodan capsule (BBD), a traditional Chinese (TCM) formula, has been widely used as an alternative remedy for multiple types of malignancies, clinically. However, the underlying mechanisms behind the efficacy of BBD remain poorly understood, particularly in regard to lung cancer. Herein, we demonstrate that BBD induc...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-08-15 00:00:00
abstract：:HOE-642 has been shown to provide significant protection in a variety of models of cerebral and myocardial ischemia/reperfusion injury. In this study, we examined the impact of HOE-642, a selective Na+/H+ exchanger 1 inhibitor, with or without hypothermia on neuronal and neuronal mitochondrial function during resuscit...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-05-15 00:00:00
abstract：:The goal of this study was to assess the role of high-mobility group box 1 (HMGB1)-induced endothelial cell (EC) pyroptosis in systemic inflammatory response syndrome (SIRS) following radiofrequency (RF) ablation of hepatic hemangiomas. We enrolled 76 patients with hepatic hemangioma who underwent RF ablation. Serum c...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-12-15 00:00:00
abstract：OBJECTIVE:To create the rabbit animal model of intervertebral disc (IVD) degeneration by the annulus needle puncture technique through a novel transabdominal approach. METHODS:Thirteen New Zealand White rabbits underwent annular puncture at the L3/4, L4/5, and L5/6 discs through a transabdominal approach. For a longit...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-03-15 00:00:00
abstract：OBJECTIVES:In the present study, we investigate the effects of Mas oncogene-related gene (Mrg) C receptors (MrgC) on the expression and activation of spinal Gi protein, N-methyl-D-aspartate receptor subunit 2B (NR2B), and neuronal nitric oxide synthase (nNOS) in mouse model of bone cancer pain. METHODS:The number of s...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
abstract：:Diabetic nephropathy (DN) is known as a major microvascular complication leading cause of end-stage renal disease, it generally followed by the process of podocyte fragmentation and detachment. Transforming growth factor β1 (TGF-β1) signaling pathway plays a pivotal role in the initiation and progression of DN. In pre...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-09-15 00:00:00
abstract：:Current knowledge of normal developmental physiology and identification of specific cell types of the kidney at molecular levels enables us to generate various cells of the kidney. The generation of renal specialized cells in vitro with its correct molecular and functional implications is the urgent need for cellular ...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2019-02-15 00:00:00
abstract：:Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase which utilizes collagen as a ligand to regulate the interaction between cancer cells and tumor stroma. However, the clinical relevance of DDR1 expression in bladder cancer as well as its molecular regulation have not been previously investigated. Here, w...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-05-15 00:00:00
abstract：:The purpose of the current study was to investigate the differences in knee kinematics and kinetics in patients with semilunar lateral meniscus (SLM) and discoid lateral meniscus (DLM) injuries before and after arthroscopic partial meniscectomy during level walking. Fifteen healthy volunteers (control group), thirteen...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-06-15 00:00:00
abstract：BACKGROUND:There is conflicting evidence regarding the prognostic value of cytotoxic T cell infiltration in breast cancer. The aims of this study were to detect the expression levels and localization of FoxP3 and CD8 in invasive ductal carcinoma of the breast and to investigate the correlations among FoxP3+ regulatory ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-08-15 00:00:00
abstract：:Intraoperative auto-transfusion with the use of cell saver systems is routinely used to reduce the rate of packed red blood transfusion in major surgery. Nevertheless some concerns have been raised on possible risks of coagulation disorders. The aim of the study was to analyze the blood processed by the cell saver, re...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:A surplus of food supply has evoked a worldwide increase in incidence of type 2 diabetes. This trend will have a significant impact on the life span of people living in modern societies. In contrast, reduced calorie intake has significant impact on preventing type 2 diabetes and increasing longevity. Increased product...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2010-06-10 00:00:00
abstract：:Staphylococcus aureus (S. aureus) peptidoglycan (PGN-sa), the major cell wall component of S. aureus, has been demonstrated to be an important virulence factor in the pathogenesis of S. aureus-induced osteomyelitis. However, the exact role of PGN-sa in osteoclastogenesis during S. aureus-induced osteomyelitis and its ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-11-15 00:00:00
abstract：BACKGROUND:Single-cell RNA sequencing (scRNA-seq) was recently adopted for exploring molecular programmes and lineage progression patterns of pathogenesis of important diseases. In this study, scRNA-seq was used to identify potential markers for chondrocytes in osteoarthritis (OA) and to explore the function of differe...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-11-15 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is a chronic disorder characterized by hepatic fat accumulation and abnormal lipid metabolism. Although miR-21 has been implicated in nonalcoholic fatty liver disease, it is unknown whether miR-21 could function as a therapeutic target. Here, we perform transfection analysis of...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-02-15 00:00:00
abstract：:Glioblastoma multiforme (GBM) is one of the most malignant and aggressive brain tumors with great amount of hyaluronan (HA) secretion and CD44 overexpression (HA receptor). CD44 has been suggested as a cancer stem cells (CSCs) marker. However, several clinical studies have indicated that CD44low glioma cell exhibit CS...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-11-15 00:00:00
abstract：:As two major non-operative methods, physiotherapy and acupuncture have been proved to be safe and effective in osteoarthritis (OA) treatment. However, only a little study focused on functions of both methods on cartilage repairing. The main goal of this research is to prove and compare effectiveness of acupuncture and...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-09-15 00:00:00
abstract：:Although abnormal expression of the long non-coding RNA (lncRNA) MEG3 has been reported in multiple cancer types, the role of MEG3 in the pathobiology of hepatocellular carcinoma (HCC) remains unknown. This study evaluated the expression of lncRNA MEG3 and a microRNA (miRNA-10a-5p) implicated in HCC metastasis in canc...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-07-15 00:00:00
abstract：:Nonalcoholic steatohepatitis (NASH) has similar clinical pathological changes to alcoholic hepatitis. It shows increased incidence and young trend year by year. Polyene phosphatidyl choline (PPC) is widely used in clinic for liver disease treatment. The effect and mechanism of PPC on NASH have not been fully elucidate...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-05-15 00:00:00
abstract：:MicroRNAs (miRNAs) are involved in the development and progression of lung cancer. MicroRNA-101 (miR-101) displays crucial properties in non-small cell lung cancer (NSCLC) by negatively regulating cell proliferation and invasion, but the underlying molecular mechanisms remain largely unknown. In this study, we found t...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-04-15 00:00:00
abstract：:LncRNAs have been shown to invovled in the development of various cancers. However, the function and potential mechanism of PTCSC3 in cervical carcinoma progression remains unknown. In this reference, we showed that PTCSC3 expression was downregulated in 4 cervical carcinoma cell lines (Caski, Siha, Hela and C4-1) com...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-11-15 00:00:00
abstract：:This study tested the hypothesis that healthy adipose-derived mesenchymal stem cell (ADMSC)-derived exosomes (HMSCEXO) and apoptotic (A) (induced by 12 h hypoxia/12 h starvation)-ADMSC-derived exosomes (AMSCEXO) were comparably effective at alleviating sepsis syndrome [SS; induced by cecal-ligation and puncture (CLP)]...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-04-15 00:00:00
abstract：:The high-grade glioma (HGG) remains as the greatest challenge for cancer management worldwide. Identification of novel therapeutics and diagnostic method is in urgent need. The V-set and immunoglobulin domain-containing protein 4 (VSIG4) is a complement receptor for C3b/iC3b and inhibits cytotoxic T lymphocytes activa...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-06-15 00:00:00
abstract：:Coronavirus disease 2019 (COVID-19) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic disease with high morbidity and mortality. Inflammatory and thrombosis are its main manifestations. As an important organ of hemofiltration metabolism, the kidney is prone to blocka...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2020-12-15 00:00:00
abstract：:Macrophages participate in all stages of sepsis and affect immune homeostasis and inflammatory processes. Small ubiquitin-like modifier (SUMO) protease SENP1 plays an important role in cellular inflammation by regulating proteins in SUMOylation. However, the roles and related mechanisms of SENP1 in macrophage inflamma...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-11-15 00:00:00
abstract：:EZH2 is the catalytic subunit of Polycomb Repressor Complex 2 (PRC2) which catalyzes methylation of histone H3 at lysine 27 (H3K27me) and mediates gene silencing of target genes via local chromatin reorganization. Numerous evidences show that EZH2 plays a critical role in cancer initiation, progression and metastasis,...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2015-02-15 00:00:00
abstract：BACKGROUND:To provide novel insight into the development of new therapeutic strategies to combat breast cancer, differentiation-inducing activity of clinically achievable concentrations of arsenite (AsIII) and tetrandrine (Tetra) was investigated in breast cancer cell lines MDA-MB-231 and MCF-7. METHODS:Differentiatio...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-12-15 00:00:00
abstract：:Spinal cord injury (SCI) can lead to different levels of paraplegia. Studies have shown that exercise exerts wide protective effects against various diseases, and microRNAs (miRNAs) are involved in its beneficial effects. However, the specific role of miRNAs in the protective effects of exercise against SCI remains un...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：:Macrophages are a major component of tumor stroma and the infiltrated macrophages in malignant tumor tissues (as called tumor associated macrophages, TAM) play a pivotal role in hepatocellular carcinoma (HCC) progression. However, the molecular mechanisms of macrophages promoting HCC metastasis are poorly understood. ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-05-15 00:00:00
