Abstract:
BACKGROUND:Prompt laboratory diagnosis and initiation of treatment are effective components of leishmaniasis control. Detection of Leishmania parasites by ex-vivo culture of lesion scrapings is considered a definitive diagnostic method preceding initiation of treatment. OBJECTIVE:A pilot study to find alternative medium that could reduce the cost of culturing from patient lesions for diagnosing leishmaniasis. METHOD:GALF-1 medium was formulated in our lab from locally available inexpensive solutions and powders in the presence of urine from healthy individuals. Amastigote to promastigote transformation, recovery of parasites after cryopreservation, cost and mass cultivation was compared using the following media: GALF-1, RPMI 1640, and conventional Locke's semi-solid medium (LSSM), a modifications of Novy-MacNeal-Nicolle culture media, which uses Locke's solution as an overlay RESULTS:GALF-1 preparation was cheap and the components available in low-income countries such as Ethiopia. Preparation was simple, not requiring autoclaving and extra distilled water. GALF-1 was able to transform amastigotes from Ethiopian patients' samples and could be used to cultivate promastigotes in large quantities. GALF-1 decreased Leishmania culture costs by approximately 80-95% compared to LSSM and RPMI 1640, respectively. Promastigotes cultured with GALF-1 could be cryopreserved in liquid nitrogen with comparable re-culture potential. CONCLUSION:Affordability of diagnostic assays is a key issue for endemic resource-poor countries and the possibility to cut the cost of the efficient culture method for diagnosis through the use of inexpensive, locally formulated reagents could improve the diagnosis of leishmaniasis in Ethiopia and in other low-income countries.
journal_name
Glob Health Actionjournal_title
Global health actionauthors
Tasew G,Kebede A,Wolday D,Gadisa E,Britton S,Eidsmo L,Akuffo Hdoi
10.3402/gha.v2i0.2046subject
Has Abstractpub_date
2009-10-22 00:00:00eissn
1654-9716issn
1654-9880journal_volume
2pub_type
杂志文章abstract:OBJECTIVE:We assessed the availability of essential medicines for mental healthcare (MH) across levels of the public healthcare system to aid in future systems planning. DESIGN:Non-expired MH medications were assessed in 24 public health facilities and 13 district warehouses across Sofala Province, Mozambique, from Ju...
journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v8.27942
更新日期:2015-06-15 00:00:00
abstract:BACKGROUND:In India, 50-65% of the population face difficulties in accessing medicines. The Health Impact Fund (HIF) is a novel proposal whereby pharmaceutical companies would be paid based on the measured global health impact of their drugs. We conducted a key stakeholder analysis to explore access to medicines in Ind...
journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2018.1434935
更新日期:2018-01-01 00:00:00
abstract:INTRODUCTION:Dengue fever is an important vector-transmitted disease that affects more than 100 countries worldwide. Locations where individuals tend to gather may play an important role in disease transmission in the presence of the vector. By controlling mosquitoes' breeding places, this study aims to analyze the eff...
journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v9.28026
更新日期:2016-01-06 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v7.24997
更新日期:2014-08-21 00:00:00
abstract::Bangladesh has historically been cholera endemic, with seasonal cholera outbreaks occurring each year. In collaboration with the government of Bangladesh, the Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) initiated operational research to test strategies to re...
journal_title:Global health action
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更新日期:2019-01-01 00:00:00
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journal_title:Global health action
pub_type: 杂志文章,评审
doi:10.1080/16549716.2016.1264702
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Hazardous alcohol use is detrimental to persons with HIV (PWH), impacting medication adherence and liver function, yet globally resources to target alcohol use behavior in this population are limited. Few studies have identified the costs of integrating alcohol reduction interventions into HIV care. OBJECTI...
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journal_title:Global health action
pub_type: 杂志文章,评审
doi:10.3402/gha.v8.28298
更新日期:2015-10-29 00:00:00
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journal_title:Global health action
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v8.26683
更新日期:2015-05-11 00:00:00
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journal_title:Global health action
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v9.30626
更新日期:2016-02-09 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v7.23887
更新日期:2014-05-12 00:00:00
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journal_title:Global health action
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1080/16549716.2017.1341597
更新日期:2017-01-01 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v7.25351
更新日期:2014-12-03 00:00:00
abstract:BACKGROUND:Support supervision is one of the strategies used to check the quality of services provided at health facilities. From 2013 to 2015, Makerere University School of Public Health strengthened support supervision in the district of Kibuku, Kamuli and Pallisa in Eastern Uganda to improve the quality of maternal ...
journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2017.1345496
更新日期:2017-08-01 00:00:00
abstract::Community-based research on child survival in sub-Saharan Africa has focussed on the increased provision of curative health services by a formalised cadre of lay community health workers (CHWs), but we have identified a particular configuration, that deserves closer scrutiny. We identified a two-tiered CHW system, wit...
journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v8.27214
更新日期:2015-03-12 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2020.1830463
更新日期:2020-12-31 00:00:00
abstract::Background: Stability of measures in quantitative social science research is crucial to understand. There is very little evidence on the stability of violence against women and girls measures in the global South. Objective: To assess the test-retest stability of violence against women and girls measures, amongst young...
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v7.24352
更新日期:2014-09-22 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
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更新日期:2020-12-31 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v3i0.5640
更新日期:2010-12-17 00:00:00
abstract:BACKGROUND:India's older population is projected to increase up to 96 million by 2011 with older people accounting for 18% of its population by 2051. The Study on Global Ageing and Adult Health aims to improve empirical understanding of health and well-being of older adults in developing countries. OBJECTIVES:To exami...
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pub_type: 杂志文章
doi:10.3402/gha.v3i0.2128
更新日期:2010-09-27 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2017.1296726
更新日期:2017-01-01 00:00:00
abstract::Ghana is classified as being in the malaria control phase, according to the global malaria elimination program. With many years of policy development and control interventions, malaria specific mortality among children less than 5 years old has declined from 14.4% in 2000 to 0.6% in 2012. However, the same level of su...
journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2017.1381471
更新日期:2017-01-01 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v3i0.2070
更新日期:2010-02-25 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.3402/gha.v6i0.20004
更新日期:2013-02-12 00:00:00
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journal_title:Global health action
pub_type: 杂志文章
doi:10.1080/16549716.2017.1410975
更新日期:2017-01-01 00:00:00