Abstract:
:The progression and variation of pathology during infections can be due to components from both host or pathogen, and/or the interaction between them. The influence of host genetic variation on disease pathology during infections with trypanosomes has been well studied in recent years, but the role of parasite genetic variation has not been extensively studied. We have shown that there is parasite strain-specific variation in the level of splenomegaly and hepatomegaly in infected mice and used a forward genetic approach to identify the parasite loci that determine this variation. This approach allowed us to dissect and identify the parasite loci that determine the complex phenotypes induced by infection. Using the available trypanosome genetic map, a major quantitative trait locus (QTL) was identified on T. brucei chromosome 3 (LOD = 7.2) that accounted for approximately two thirds of the variance observed in each of two correlated phenotypes, splenomegaly and hepatomegaly, in the infected mice (named TbOrg1). In addition, a second locus was identified that contributed to splenomegaly, hepatomegaly and reticulocytosis (TbOrg2). This is the first use of quantitative trait locus mapping in a diploid protozoan and shows that there are trypanosome genes that directly contribute to the progression of pathology during infections and, therefore, that parasite genetic variation can be a critical factor in disease outcome. The identification of parasite loci is a first step towards identifying the genes that are responsible for these important traits and shows the power of genetic analysis as a tool for dissecting complex quantitative phenotypic traits.
journal_name
PLoS Negl Trop Disjournal_title
PLoS neglected tropical diseasesauthors
Morrison LJ,Tait A,McLellan S,Sweeney L,Turner CM,MacLeod Adoi
10.1371/journal.pntd.0000557subject
Has Abstractpub_date
2009-12-01 00:00:00pages
e557issue
12eissn
1935-2727issn
1935-2735journal_volume
3pub_type
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,meta分析,评审
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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pub_type: 历史文章,杂志文章
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pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0005746
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pub_type: 杂志文章,meta分析,评审
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更新日期:2013-05-02 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,meta分析
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2020-02-18 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0006675
更新日期:2018-07-26 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,多中心研究
doi:10.1371/journal.pntd.0007495
更新日期:2019-06-27 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2017-05-18 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2014-05-22 00:00:00
abstract:BACKGROUND:New strategies for collecting post-mortem tissue are necessary, particularly in areas with emerging infections. Minimally invasive autopsy (MIA) has been proposed as an alternative to conventional autopsy (CA), with promising results. Previous studies using MIA addressed the cause of death in adults and chil...
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0007625
更新日期:2019-07-22 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0004144
更新日期:2015-10-16 00:00:00