Abstract：
BACKGROUND:Benzodiazepine binding sites distinct from the GABA-receptor-chloride-complex in the central nervous system have been recognized in many peripheral tissues, but their physiological role remains unexplained. Our study was undertaken to examine the effects of diazepam, clonazepam, and PK 11195, a peripheral benzodiazepine receptor antagonist, on the functional and biochemical responses of platelets and neutrophils stimulated by different physiological agonists. MATERIAL/METHODS:The experiments were conducted on isolated washed rat platelets activated by arachidonic acid (AA), adenosine 5'-diphosphate (ADP), or thrombin and on isolated rat neutrophils activated by a chemotactic peptide, formyl methionyl leucyl phenylalanine (fMLP). RESULTS:The results showed that neither diazepam nor clonazepam nor PK 11195 alone augmented the response of resting platelets or modified neutrophil response, but diazepam and clonazepam in a concentration-dependent manner inhibited thrombin, ADP or AA-stimulated platelet aggregation and the thrombin-induced increase in free intracellular Ca2+. Both drugs also exerted an inhibitory effect on reactive oxygen species (ROS) produced by fMLP-stimulated neutrophils. However, diazepam was about 10 times more effective than clonazepam. PK11195 did not influence platelet and neutrophil function stimulated by agonists, but reversed the inhibitory action of both benzodiazepines on platelet activation and ROS production. CONCLUSIONS:The results indicated that in vitro diazepam, and in a much smaller degree clonazepam, may down-regulate platelet activation and release of some proinflammatory mediators by stimulated neutrophils. These effects are probably exerted by a specific benzodiazepine binding sites.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Rajtar G,Zółkowska D,Kleinrok Zsubject
Has Abstractpub_date
2002-04-01 00:00:00pages
PI37-44issue
4eissn
1234-1010issn
1643-3750pii
1419journal_volume
8pub_type
杂志文章abstract：:BACKGROUND Dilated cardiomyopathy (DCM), which is characterized by enlarged ventricular dimensions and systolic dysfunction, is the most common type of cardiomyopathy. Mutations in the LMNA gene are reported in approximately 10% of familial DCM cases. However, the mechanism of LMNA mutations in human DCM remains uncle...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.924576
更新日期：2020-06-14 00:00:00
abstract：:BACKGROUND Studies on monoamine oxidase B (MAO-B) expression in renal cell carcinoma (RCC) are lacking. This study focused on the immunohistochemical evaluation of MAO-B in RCC. MATERIAL AND METHODS Sixty-three RCC samples were compared on basic clinical and histopathological parameters, including histopathological ty...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.909507
更新日期：2018-08-04 00:00:00
abstract：BACKGROUND:Ventricular long axis function has recently been proved to be of prognostic value in patients with heart failure. However, it remains unclear whether it can provide additional independent information over that provided by conventionally used LVEF or functional status. We conducted this study to address this ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2003-06-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-02-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,多中心研究
doi：10.12659/msm.897638
更新日期：2016-04-11 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.894926
更新日期：2015-08-28 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.889946
更新日期：2014-01-22 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.897443
更新日期：2016-03-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2004-04-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
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更新日期：2015-03-14 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.922659
更新日期：2020-07-21 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2019-10-20 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.898454
更新日期：2016-12-16 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type：
doi：
更新日期：2009-02-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.889443
更新日期：2013-09-09 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.927440
更新日期：2021-01-02 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.892711
更新日期：2014-12-30 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2005-07-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.921026
更新日期：2020-03-02 00:00:00
abstract：BACKGROUND:There is evidence that myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by activation of immune, inflammatory, oxidative and nitrosative stress (IO&NS) pathways. The present study was carried out in order to examine whether ME/CFS is accompanied by increased levels of plasma perox...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.881699
更新日期：2011-04-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2005-04-01 00:00:00
