miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts.

Abstract:

:BACKGROUND Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL AND METHODS Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration. RESULTS miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts. CONCLUSIONS Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.

journal_name

Med Sci Monit

authors

Ying J,Liu Y,Yang R,Zhang Y,Xu J

doi

10.12659/msm.897443

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

697-701

eissn

1234-1010

issn

1643-3750

pii

897443

journal_volume

22

pub_type

杂志文章
  • Diagnostic Value of Semiquantitative Analysis of 99mTechnetium-Methoxyisobutylisonitrile (99mTc-MIBI) Imaging in Predicting Early-Stage Cervical Lymph Node Metastasis of Thyroid Carcinoma.

    abstract::BACKGROUND The aim of this study was to evaluate the value of semiquantitative analysis (SQA) of 99mTc-MIBI imaging in predicting early-stage cervical lymph node metastasis (CLNM) in thyroid carcinoma (TC). MATERIAL AND METHODS TC patients (n =106) undergoing surgical resection and histopathological examination were e...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.899966

    authors: Zhu XC,Zhou K,Xu SQ,Ma YB

    更新日期:2017-03-31 00:00:00

  • Psychological distress in Brazilian Crohn's disease patients: screening, prevalence, and risk factors.

    abstract:BACKGROUND:Psychological disturbances are frequent in Crohn's disease (CD) patients. However, epidemiological studies of non-western CD populations are limited and may be confounded by genetic and disease-related influences. The aim of this study was to assess the prevalence and risk factors for depression and symptoms...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Brandi MT,Ribeiro MS,Chebli LA,Franco MB,Pinto AL,Gaburri PD,Zanini A,Chebli JM

    更新日期:2009-08-01 00:00:00

  • Comparison of Clinical Efficacy Between Modular Cementless Stem Prostheses and Coated Cementless Long-Stem Prostheses on Bone Defect in Hip Revision Arthroplasty.

    abstract::BACKGROUND The aim of this study was to investigate and compare the clinical efficacy of modular cementless stem and coated cementless long-stem prostheses in hip revision arthroplasty. MATERIAL AND METHODS Sixty-five patients with complete hip revision surgery data during January 2005 to March 2015 were selected from...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,随机对照试验

    doi:10.12659/msm.895709

    authors: Li H,Chen F,Wang Z,Chen Q

    更新日期:2016-02-29 00:00:00

  • The effects of medical treatment and rehabilitation in a patient with adult tethered cord syndrome in the late postoperative period.

    abstract:BACKGROUND:The possibility of functional tethered cord syndrome (TCS) should be considered when adolescent patients present with unexplained lumbar, buttock, and leg symptoms. Symptoms progress if untreated. Success depends on early diagnosis, the complete untethering of the spinal cord, and rehabilitation. A rare case...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Kabayel DD,Ozdemir F,Unlu E,Bilgili N,Murat S

    更新日期:2007-12-01 00:00:00

  • Fibrinogen-Like Protein 2 (FGL2) is a Novel Biomarker for Clinical Prediction of Human Breast Cancer.

    abstract::BACKGROUND Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients. However, the...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.923531

    authors: Feng Y,Guo C,Wang H,Zhao L,Wang W,Wang T,Feng Y,Yuan K,Huang G

    更新日期:2020-07-27 00:00:00

  • Common Polymorphisms in the NFKBIA Gene and Cancer Susceptibility: A Meta-Analysis.

    abstract:BACKGROUND:NFKBIA encodes the inhibitors of nuclear factor-κB (NF-κB), which regulate the translation of the genes involved in the inflammatory and immune reactions. Polymorphisms (rs2233406, rs3138053, and rs696) of NFKBIA have been implicated in susceptibility to many cancer types. MATERIAL AND METHODS:To evaluate t...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,meta分析

    doi:10.12659/msm.895257

    authors: Zhang M,Huang J,Tan X,Bai J,Wang H,Ge Y,Xiong H,Shi J,Lu W,Lv Z,Liang C

    更新日期:2015-10-21 00:00:00

  • Clinical and Radiological Presentations and Management of Blunt Splenic Trauma: A Single Tertiary Hospital Experience.

    abstract::BACKGROUND Splenic injury is the leading cause of major bleeding after blunt abdominal trauma. We examined the clinical and radiological presentations, management, and outcome of blunt splenic injuries (BSI) in our institution. MATERIAL AND METHODS A retrospective study of BSI patients between 2011 and 2014 was conduc...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.902438

    authors: Jabbour G,Al-Hassani A,El-Menyar A,Abdelrahman H,Peralta R,Ellabib M,Al-Jogol H,Asim M,Al-Thani H

    更新日期:2017-07-12 00:00:00

  • Game-Based Virtual Reality Canoe Paddling Training to Improve Postural Balance and Upper Extremity Function: A Preliminary Randomized Controlled Study of 30 Patients with Subacute Stroke.

    abstract::BACKGROUND Virtual reality (VR) training with motion-controlled console games can be incorporated into stroke rehabilitation programs. The use of a variety of gaming software can provide the patient with an opportunity to perform activities that are exciting, entertaining, and that may not be feasible in clinical envi...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,随机对照试验

    doi:10.12659/MSM.906451

    authors: Lee MM,Lee KJ,Song CH

    更新日期:2018-04-27 00:00:00

  • Correlation Between Interleukin-1β-511 C/T Polymorphism and Gastric Cancer in Chinese Populations: A Meta-Analysis.

    abstract::BACKGROUND Several studies have indicated that interleukin (IL)-1β-511 C/T polymorphism may contribute to individual susceptibility to gastric cancer, but the results vary among regions and races. No relevant meta-analysis has been conducted in a Chinese population. Therefore, we performed the current meta-analysis to...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,meta分析

    doi:10.12659/msm.895771

    authors: Chen B,Luo MX,Zhou X,Lv Y,Su GQ

    更新日期:2016-05-24 00:00:00

  • Level of TNF-related apoptosis-inducing-ligand and CXCL8 correlated with 2-[18F]Fluoro-2-deoxy-D-glucose uptake in anti-VEGF treated colon cancers.

    abstract:BACKGROUND:The changes and correlations of TRAIL (TNF-related apoptosis-inducing-ligand) and CXCL8 (IL8) prior to treatment and three months following therapy as well as the corresponding Positron emission tomography (PET/CT) (SUV(max): standardized uptake maximum values) results were evaluated. MATERIAL AND METHODS:T...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.889605

    authors: Celik B,Yalcin AD,Bisgin A,Dimitrakopoulou-Strauss A,Kargi A,Strauss LG

    更新日期:2013-10-21 00:00:00

  • Prolonged unintended brain cooling may inhibit recovery from brain injuries: case study and literature review.

    abstract:BACKGROUND:Tracheal intubation of comatose patients is common, but contrary to most standards for respiratory care, heated nebulizers are not always used. This deviation from recommendations appears to be widespread. CASE REPORT:In the case examined, a tracheotomized patient suffering from severe anoxic brain injury w...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:

    authors: Ford GP,Reardon DC

    更新日期:2006-08-01 00:00:00

  • Integrating theories of the etiology of Crohn's disease. On the etiology of Crohn's disease: questioning the hypotheses.

    abstract::The most prominent theory describes the Crohn's Syndrome as a dysregulated, inappropriate immune response to otherwise innocuous bowel flora in a genetically susceptible host. The autoimmune theory assumes that a specific infectious agent does not exist. Data from mouse models, impairment of the mucosal epithelial bar...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章,评审

    doi:

    authors: Chamberlin WM,Naser SA

    更新日期:2006-02-01 00:00:00

  • Virologic response to treatment with Pegylated Interferon alfa-2b and Ribavirin for chronic hepatitis C in children.

    abstract:BACKGROUND:This study assessed the efficacy and safety of treatment of chronic hepatitis C in children with pegylated interferon alpha and ribavirin. MATERIAL/METHODS:Investigations were performed on 53 children with chronic hepatitis C, aged 8-17 years. Children were divided into 2 groups: naïve (n=29) and retreated ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Pawłowska M,Pilarczyk M,Halota W

    更新日期:2010-12-01 00:00:00

  • Expression of Th-17 and RORγt mRNA in Behçet's Disease.

    abstract:BACKGROUND:To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD). MATERIAL/METHODS:Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosi...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.881720

    authors: Hamzaoui K,Bouali E,Ghorbel I,Khanfir M,Houman H,Hamzaoui A

    更新日期:2011-04-01 00:00:00

  • Metformin or Acarbose Treatment Significantly Reduced Albuminuria in Patients with Newly Diagnosed Type 2 Diabetes Mellitus and Low-Grade Albuminuria.

    abstract::BACKGROUND A urine albumin to creatinine ratio (UACR) >30 mg/g is considered to represent albuminuria, but in type 2 diabetes mellitus, even low-grade albuminuria is associated with increased risk of cardiovascular disease. This study aimed to investigate the effects of metformin and acarbose treatment on urine albumi...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.911979

    authors: Pan Q,Xu Y,Yang N,Gao X,Liu J,Yang W,Wang G

    更新日期:2018-12-10 00:00:00

  • White and Gray Matter Volume Changes and Correlation with Visual Evoked Potential in Patients with Optic Neuritis: A Voxel-Based Morphometry Study.

    abstract:BACKGROUND:The aim of this study was to investigate potential morphological alterations of gray and white matter in patients with optic neuritis (ON) and their relationship with behavioral performance, using voxel-based morphometry (VBM). MATERIAL/METHODS:Twelve (4 males, 8 females) patients with ON and 12 (4 males, 8...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.897837

    authors: Huang X,Zhang Q,Hu PH,Zhong YL,Zhang Y,Wei R,Xu TT,Shao Y,Oculopathy fMRI study group.

    更新日期:2016-04-05 00:00:00

  • Laparoscopic surgery for diagnosis and treatment of bowel obstruction: case report of paracecal hernia.

    abstract:BACKGROUND:Small bowel obstruction cased by paracecal hernia is rare, and the condition is difficult to diagnose preoperatively. We herein report a case of paracecal hernia that was successfully diagnosed and treated with laparoscopic surgery. CASE REPORT:A 74-year-old man admitted our hospital. His previous medical h...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Hirokawa T,Hayakawa T,Tanaka M,Okada Y,Sawai H,Takeyama H,Manabe T

    更新日期:2007-07-01 00:00:00

  • Expression of KIF18A Is Associated with Increased Tumor Stage and Cell Proliferation in Prostate Cancer.

    abstract::BACKGROUND The role of KIF18A in tumorigenesis and tumor development has been well studied in several cancers, but not in prostate cancer. In this study, we investigated the potential prognostic utility of KIF18A and its role in prostate cancer progression. MATERIAL AND METHODS We collected prostate cancer and paracan...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.917352

    authors: Zhang H,Shen T,Zhang Z,Li Y,Pan Z

    更新日期:2019-08-27 00:00:00

  • Anterograde Fixation Module for Posterior Acetabular Column Fracture: Computer-Assisted Determination of Optimal Entry Point, Angle, and Length for Screw Insertion.

    abstract::BACKGROUND The aim of this study was to provide valid data for a plate-screw fixation model for fractured posterior-anterior columns of the acetabulum. MATERIAL AND METHODS Nineteen cadaveric bony hemi-pelvis specimens were obtained and 50 healthy adults were enrolled. The modified Stoppa approach and computed tomogra...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.896313

    authors: Xu Y,Lin C,Zhang L,Lin M,Lai J,Cao S,Peng G,Feng K,Yan G,Cai D,Wang G

    更新日期:2016-09-01 00:00:00

  • High Human Cytomegalovirus IgG Level is Associated with Increased Incidence of Diabetic Atherosclerosis in Type 2 Diabetes Mellitus Patients.

    abstract::BACKGROUND At present, whether human cytomegalovirus (HCMV) infection is associated with type 2 diabetes mellitus (T2DM) is debatable. The effect of active HCMV infection on glucose regulation has been poorly studied. Although HCMV infection is correlated with atherosclerosis in cardiovascular disease, the role of HCM...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.896071

    authors: Zhang J,Liu YY,Sun HL,Li S,Xiong HR,Yang ZQ,Xiang GD,Jiang XJ

    更新日期:2015-12-30 00:00:00

  • Sexual dysfunction after curative radical resection of rectal cancer in men: the role of extended systematic lymph-node dissection.

    abstract:BACKGROUND:This study was designed to determine the incidence and patterns of sexual dysfunction after curative radical resections (CRR) with or without extended systematic lymph-node dissection (ESLND) for rectal cancer Material/Methods: A total of 91 patients with rectal cancer were reviewed with respect to surgical ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Cöl C,Hasdemir O,Yalçin E,Yandakçi K,Tunç G,Kuçukpinar T

    更新日期:2006-02-01 00:00:00

  • Sepsis induced by Staphylococcus aureus: participation of biomarkers in a murine model.

    abstract:BACKGROUND:This study aimed to evaluate the role of biomarkers in the pathophysiological process induced by a Staphylococcus aureus strain obtained in a hospital environment. For this, we intraperitoneally inoculated groups of male BALB/c mice with S. aureus, using a clinical isolate (CI) of S. aureus. MATERIAL/METHOD...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.892528

    authors: de Oliveira TH,Amorin AT,Rezende IS,Santos Barbosa M,Martins HB,Brito AK,Andrade EF,Gonçalves GK,Campos GB,Silva RA,Timenetsky J,Marques LM

    更新日期:2015-01-29 00:00:00

  • 3-D CT and MRI co-registration in the assessment of cochlear implantation.

    abstract:BACKGROUND:The aim of this study was to evaluate the feasibility of co-registering preoperative MRI and postoperative CT and to assess the position of the electrode within the cochlea. MATERIAL/METHODS:In a series of 14 patients, high-resolution MRI datasets of the cochlea obtained before implantation and multirow CT ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Neri E,Berrettini S,Salvatori L,Forli F,Franceschini SS,Bartolozzi C

    更新日期:2005-10-01 00:00:00

  • Oxidative DNA Damage to Sperm Cells and Peripheral Blood Leukocytes in Infertile Men.

    abstract::BACKGROUND Oxidative DNA damage is associated with male infertility. The aim of this study was to evaluate the oxidative DNA damage of sperm cells and blood leukocytes and to determine the levels of MDA and NO levels in seminal and blood plasma of idiopathic infertile men. MATERIAL AND METHODS The study enrolled 52 pa...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.898631

    authors: Taken K,Alp HH,Eryilmaz R,Donmez MI,Demir M,Gunes M,Aslan R,Sekeroglu MR

    更新日期:2016-11-11 00:00:00

  • Ultrasound investigation of the glenohumeral joint by anterior access in patients with rheumatoid arthritis and healthy controls.

    abstract:BACKGROUND:The aim of this study was to measure glenohumeral joint (GHJ) parameters via the anterior access through ultrasound and to compare to data from posterior and inferior accesses. MATERIAL AND METHODS:Twenty healthy controls (M: F=15: 5, aged 45.1±11.2 years) and 16 patients (M: F=5: 11, aged 54.6±14.7 years) ...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.892520

    authors: Rozin AP,Toledano K,Dagan A,Balbir-Gurman A

    更新日期:2015-02-18 00:00:00

  • Clinical Features and Advantages of a Novel Percutaneous Endoscopic Gastrostomy Method.

    abstract::BACKGROUND To study the clinical characteristics of novel percutaneous endoscopic gastrostomy. MATERIAL AND METHODS We retrospectively analyzed the hospital records of 173 patients undergoing various methods of gastrostomy (a novel PEG, traditional PEG, and surgical gastrostomy). Clinical characteristics were analyzed...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.918507

    authors: Cui N,Zhao Y,Cao J

    更新日期:2019-12-17 00:00:00

  • Relationship Between Carotid Stenosis and Infarct Volume in Ischemic Stroke Patients.

    abstract::BACKGROUND Stroke is a serious health problem all over the world. Ischemia causes 85% of strokes and 75% of these ischemic strokes occur within the area supplied by the internal carotid artery (ICA). MATERIAL AND METHODS This study included 47 acute stroke patients who were in the large-artery atherosclerosis group ac...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.898112

    authors: Alagoz AN,Acar BA,Acar T,Karacan A,Demiryürek BE

    更新日期:2016-12-16 00:00:00

  • Prediction of early HBeAg seroconversion by decreased titers of HBeAg in the serum combined with increased grades of lobular inflammation in the liver.

    abstract:BACKGROUND:Hepatitis B e antigen (HBeAg) seroconversion is an important hallmark in the natural course of chronic hepatitis B. This study was designed to predict early HBeAg seroconversion within 1 year, by not only biochemical and virological markers, but also pathological parameters in patients with chronic hepatitis...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/msm.883595

    authors: Bae SK,Yatsuhashi H,Hashimoto S,Motoyoshi Y,Ozawa E,Nagaoka S,Abiru S,Komori A,Migita K,Nakamura M,Ito M,Miyakawa Y,Ishibashi H

    更新日期:2012-12-01 00:00:00

  • Influence of selected angiotensin-converting enzyme inhibitors on alloxan-induced diabetic cataract in rabbits.

    abstract:BACKGROUND:Hyperglycemia enhances cataractogenesis. Elevated glucose level is commonly accompanied by arterial hypertension, for which angiotensin-converting enzyme (ACE) inhibitors (ACEIs) are a widely used intervention. ACE inhibitors exert some endothelial pleiotropic actions and can beneficially modulate glucose co...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:

    authors: Jablecka A,Czaplicka E,Olszewski J,Bogdanski P,Krauss H,Smolarek I

    更新日期:2009-11-01 00:00:00

  • Efficacy and safety of recombinant Mycobacterium tuberculosis ESAT-6 protein for diagnosis of pulmonary tuberculosis: a phase II trial.

    abstract:BACKGROUND:This study aimed to determine the efficacy and safety of recombinant Mycobacterium tuberculosis ESAT-6 protein for diagnosis of pulmonary tuberculosis (TB). MATERIAL AND METHODS:A phase II trial was performed in 158 patients with pulmonary TB (145 initially-treated and 13 re-treated) and 133 healthy subject...

    journal_title:Medical science monitor : international medical journal of experimental and clinical research

    pub_type: 杂志文章

    doi:10.12659/MSM.889425

    authors: Sun QF,Xu M,Wu JG,Chen BW,Du WX,Ding JG,Shen XB,Su C,Wen JS,Wang GZ

    更新日期:2013-11-12 00:00:00