Abstract:
BACKGROUND:Trichinella nematodes are globally distributed food-borne pathogens, in which Trichinella spiralis is the most common species in China. Microsatellites are a powerful tool in population genetics and phylogeographic analysis. However, only a few microsatellite markers were reported in T. spiralis. Thus, there is a need to develop and validate genome-wide microsatellite markers for T. spiralis. METHODS:Microsatellites were selected from shotgun genomic sequences using MIcroSAtellite identification tool (MISA). The identified markers were validated in 12 isolates of T. spiralis in China. RESULTS:A total of 93,140 microsatellites were identified by MISA from 9267 contigs in T. spiralis genome sequences, in which 16 polymorphic loci were selected for validation by PCR with single larvae from 12 isolates of T. spiralis in China. There were 7-19 alleles per locus (average 11.25 alleles per locus). The observed heterozygosity (HO) and expected heterozygosity (HE) ranged from 0.325 to 0.750 and 0.737 to 0.918, respectively. The polymorphism information content (PIC) ranged from 0.719 to 0.978 (average 0.826). Among the 16 loci, markers for 10 loci could be amplified from all 12 international standard strains of Trichinella spp. CONCLUSIONS:Sixteen highly polymorphic markers were selected and validated for T. spiralis. Primary phylogenetic analysis showed that these markers might serve as a useful tool for genetic studies of Trichinella parasites.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Li TT,Tang B,Bai X,Wang XL,Luo XN,Yan HB,Zhu HF,Jia H,Liu XL,Liu MYdoi
10.1186/s13071-020-3929-2subject
Has Abstractpub_date
2020-02-11 00:00:00pages
58issue
1issn
1756-3305pii
10.1186/s13071-020-3929-2journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Malaria vector control in Africa relies on insecticides targeting adult mosquito vectors via insecticide treated nets or indoor residual spraying. Despite the proven efficacy of these strategies, the emergence and rapid rise in insecticide resistance in malaria vectors raises many concerns about their sustai...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2016-02-05 00:00:00
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-372
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2011-05-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 信件
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-3-37
更新日期:2010-04-13 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0679-7
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1842-5
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journal_title:Parasites & vectors
pub_type: 临床试验,杂志文章
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更新日期:2018-12-20 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2763-2
更新日期:2018-03-13 00:00:00
abstract:BACKGROUND:The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-rearing facility) or import the st...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0869-3
更新日期:2015-05-01 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0476-8
更新日期:2014-10-12 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-215
更新日期:2012-09-28 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2656-4
更新日期:2018-01-31 00:00:00
abstract:BACKGROUND:Infective larvae of the worldwide occurring zoonotic roundworm T. canis exhibit a marked affinity to the nervous tissues of paratenic hosts. In humans, most cases of neurotoxocarosis are considered to be caused by larvae of T. canis as T. cati larvae have rarely been found in the CNS in previous studies. How...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-194
更新日期:2014-04-22 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3109-9
更新日期:2018-10-04 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2691-1
更新日期:2018-02-20 00:00:00
abstract:BACKGROUND:The chemical control of the mosquito Aedes aegypti, the major vector of dengue, is being seriously threatened due to the development of pyrethroid resistance. Substitutions in the 1016 and 1534 sites of the voltage gated sodium channel (AaNaV), commonly known as kdr mutations, confer the mosquito with knockd...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-25
更新日期:2014-01-15 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3096-x
更新日期:2018-10-10 00:00:00
abstract:BACKGROUND:A cytogenetic analysis of the new local triploid population of the caryophyllidean tapeworm Atractolytocestus huronensis, a unique parthenogenetic species with the ability to colonise new regions, was performed to understand the inner structure of its chromosome complement. METHODS:A karyotype analysis was ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3420-0
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Pig keeping is becoming increasingly common across sub-Saharan Africa. Domestic pigs from the Arusha region of northern Tanzania were screened for trypanosomes using PCR-based methods to examine the role of pigs as a reservoir of human and animal trypanosomiasis. METHODS:A total of 168 blood samples were ob...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-322
更新日期:2013-11-09 00:00:00
abstract:BACKGROUND:Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1687-y
更新日期:2016-07-25 00:00:00
abstract:BACKGROUND:Feline babesiosis, sporadically reported from various countries, is of major clinical significance in South Africa, particularly in certain coastal areas. Babesia felis, B. leo, B. lengau and B. microti have been reported from domestic cats in South Africa. Blood specimens from domestic cats (n = 18) showing...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3395-x
更新日期:2019-03-26 00:00:00
abstract:BACKGROUND:Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2148-y
更新日期:2017-04-24 00:00:00
abstract:BACKGROUND:Aedes mosquito-transmitted outbreaks of dengue and yellow fever have been reported from rural and urban parts of Côte d'Ivoire. The present study aimed at assessing Aedes spp. oviposition ecology in variously urbanized settings within arbovirus foci in southeastern Côte d'Ivoire. METHODS:Aedes spp. eggs wer...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1778-9
更新日期:2016-09-29 00:00:00