Abstract：
:In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Grande G,Vincenzoni F,Mancini F,Barrachina F,Giampietro A,Castagnola M,Urbani A,Oliva R,Milardi D,Pontecorvi Adoi
10.3390/jcm8122128subject
Has Abstractpub_date
2019-12-03 00:00:00issue
12issn
2077-0383pii
jcm8122128journal_volume
8pub_type
杂志文章abstract：:This report describes the trends of chorionic villus sampling (CVS) referred for prenatal genetic diagnosis in the past two and a half decades in a Portuguese Center. Our cohort of 491 CVS was mostly performed by the transcervical method at the 12th gestational week. Data collected within the framework of this study r...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm3030838
更新日期：2014-07-24 00:00:00
abstract：:Staphylococcus aureus bloodstream infection (SA-BSI) is an infection with increasing morbidity and mortality. Concomitant Staphylococcus aureus bacteriuria (SABU) frequently occurs in patients with SA-BSI. It is considered as either a sign of exacerbation of SA-BSI or a primary source in terms of urosepsis. The clinic...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9020508
更新日期：2020-02-13 00:00:00
abstract：:Several conditions can lead to the development of a subchondral cyst. The mechanism by which the cysts form, their location, and their severity depend on the underlying pathology, although the exact pathogenesis is not fully elucidated. Treatment options vary according to the location of the cyst, with less invasive p...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051358
更新日期：2020-05-06 00:00:00
abstract：:In the past, numerous non-operative treatments for adolescent idiopathic scoliosis (AIS), including exercise, physical therapy, electrical stimulation, and brace treatment, have been tried to delay or prevent the curve progression. Of these, brace treatment is the only option that is widely accepted and has demonstrat...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7060136
更新日期：2018-06-04 00:00:00
abstract：BACKGROUND:Metabolic syndrome is associated with low-grade systemic inflammation, which is a key driver of premature atherosclerosis. We characterized immune cell behavior in metabolic syndrome, its consequences, and the potential involvement of the CX3CL1/CX3CR1 and CCL2/CCR2 chemokine axes. METHODS:Whole blood from ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8050708
更新日期：2019-05-18 00:00:00
abstract：:We evaluated the incidence of temporomandibular disorder (TMD) in patients with rheumatoid arthritis (RA) and examined the association between TMD and RA, through longitudinal follow-up. Population data from the Korean National Health Insurance Service-Health Screening Cohort from 2002 to 2015 was used. From 514,866 s...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9093005
更新日期：2020-09-17 00:00:00
abstract：BACKGROUND:Compared to typically developing individuals, individuals with attention-deficit-hyperactivity disorder (ADHD) are on average more often exposed to stressful conditions (e.g., school failure, family conflicts, financial problems). We hypothesized that high exposure to stress relates to a more persistent and ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8111824
更新日期：2019-11-01 00:00:00
abstract：:Fetal exposure to particulate matter (PM) is associated with infant developmental delay likely via neuroinflammation and prefrontal cortex lesions; however, whether air-purifier usage, which can reduce indoor PM levels, is related to infant developmental delay remains unknown. We therefore examined the prospective rel...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061924
更新日期：2020-06-19 00:00:00
abstract：:Nitric oxide (NO) synthesis markers, comprising L-homoarginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), are significantly associated with cardiovascular events and mortality. Being involved in NO pathways, they may be of high importance regulating vascular tone and arterial hypertensi...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9123895
更新日期：2020-11-30 00:00:00
abstract：:Here we describe our modified surgical technique of bilateral pubococcygeus plication (BPCP) for cystocele repair and assess its safety and long-term outcomes. This is a prospective study of 147 consecutive women who underwent BPCP for cystocele between January 2010 to January 2018. Inclusion criteria was naïve women ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103318
更新日期：2020-10-16 00:00:00
abstract：:: Protein-bound uremic toxin is a cardiovascular (CV) risk factor for patients with end-stage renal disease. Indole-3-acetic acid (IAA) was found to be associated with CV disease but the detailed pathophysiology remains unknown. Moreover, mitogen-activated protein kinase (MAPK) signaling cascades play an important rol...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9010124
更新日期：2020-01-02 00:00:00
abstract：BACKGROUND:We investigated the associations between v-Raf murine sarcoma viral oncogene homolog B1 (BRAFV600E, henceforth BRAF) and v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations and colorectal cancer (CRC) prognosis, using The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GSE39582)...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8010111
更新日期：2019-01-17 00:00:00
abstract：:A recent study suggested mortality benefits using vitamin C, hydrocortisone, and thiamine combination therapy (triple therapy) in addition to standard care in patients with severe sepsis and septic shock. In order to further evaluate the effects of triple therapy in real-world clinical practice, we conducted a retrosp...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040478
更新日期：2019-04-09 00:00:00
abstract：:This study aimed to understand whether the effect of non-metastatic cells 1 (NME1) on recurrence-free survival (RFS) in early stage non-small cell lung cancer (NSCLC) can be modified by β-catenin overexpression and cisplatin-based adjuvant chemotherapy. Expression levels of NME1 and β-catenin were analyzed using immun...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103067
更新日期：2020-09-23 00:00:00
abstract：:To analyse the predictive ability and incremental value of left ventricular longitudinal axis strain (LAS) and late gadolinium enhancement (LGE) using standard cardiovascular magnetic resonance (CMR) imaging for the diagnosis and prognosis of severe aortic stenosis (AS) in patients with an indication for aortic valve ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8020165
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:Congenital factor (F) VII deficiency is a rare coagulation factor deficiency with an estimated incidence of 1 per 500,000 individuals. Patients with severe FVII deficiency present a broad range of clinical presentations. Alloimmunization against exogenous FVII, as the main challenge of replacement therapy, i...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10020211
更新日期：2021-01-08 00:00:00
abstract：:The aim of this study was to investigate the prescribing cascade phenomenon of dopaminergic drugs such as levodopa in the management of gastroprokinetic drugs induced parkinsonism. Based on the Korea National Health Insurance Service (NHIS)-Senior Cohort Database, we analyzed patients aged ≥65 years, between 2009 and ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8091496
更新日期：2019-09-19 00:00:00
abstract：:Community-acquired pneumonia (CAP) remains the first cause of morbidity and mortality worldwide due to infection. Several aspects such as severity and host response are related to its clinical course and outcome. Beyond the acute implications that the infection provokes in the host, pneumonia also has long-term negati...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9020549
更新日期：2020-02-18 00:00:00
abstract：:Injury is a major cause of worldwide child mortality. This retrospective nationwide study aimed to evaluate the characteristics of paediatric injured patients in Japan and their in-hospital mortality trends from 2009 to 2018. Injured patients aged <17 years were enrolled. Data were extracted from the Japan Trauma Data...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103273
更新日期：2020-10-12 00:00:00
abstract：BACKGROUND:Exocrine pancreatic insufficiency results from the destruction of the pancreatic parenchyma and is diagnosed by using direct or indirect tests, both of which have shortcomings. Chronic pancreatitis is the most frequent cause of this pathology in adults. METHODS:Patients meeting radiological or histological ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9062014
更新日期：2020-06-26 00:00:00
abstract：:Fine needle aspiration cytology (FNAC) is an important diagnostic tool for tumors of the head and neck. However, non-diagnostic or inconclusive results may occur and lead to delay in treatment. The aim of this study was to evaluate the factors that predict a successful FNAC. A retrospective search was performed to ide...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8122050
更新日期：2019-11-22 00:00:00
abstract：:The pathophysiology of cardiogenic shock (CS) varies depending on its etiology, which may lead to different hemodynamic profiles (HP) and may help tailor therapy. We aimed to assess the HP of CS patients according to their etiologies of acute myocardial infarction (AMI) and acute decompensated chronic heart failure (A...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113384
更新日期：2020-10-22 00:00:00
abstract：:The use of sugammadex can reduce post-operative residual neuromuscular blockade, which is known to increase the risk of post-operative respiratory events. However, its effect on post-operative pulmonary complications is not obvious. This study was performed to evaluate the effects of sugammadex on post-operative pulmo...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041232
更新日期：2020-04-24 00:00:00
abstract：:Liver transplantation is increasingly dependent on the use of extended criteria donors (ECD) to increase the organ donor pool and address rising demand. This has necessitated the adoption of innovative technologies and strategies to protect these higher-risk grafts from the deleterious effects of traditional preservat...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9041046
更新日期：2020-04-07 00:00:00
abstract：CONTEXT:As the food industry is continually involved in the development of new attractive alternative therapeutic agents, the evaluation of the beneficial impact of (poly)phenols on cognitive and brain function during aging has gained increasing interest. OBJECTIVE:This systematic review and meta-analysis aimed to eva...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9030835
更新日期：2020-03-19 00:00:00
abstract：:Arteriogenesis supplies oxygen and nutrients in the tumor microenvironment (TME), which may play an important role in tumor growth and metastasis. Pancreatic neuroendocrine tumors (pNETs) are the second most common pancreatic malignancy and are frequently metastatic on presentation. Nearly a third of pNETs secrete bio...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8111980
更新日期：2019-11-14 00:00:00
abstract：:We report the case of a 21-year old woman presenting with high blood pressure and raised normetanephrine levels. Indium-111-pentetreotide single photon-emission computed tomography with computed tomography (SPECT/CT) and 2-deoxy-2-[fluorine-18]fluoro-d-glucose (FDG) positron emission tomography/computed tomography (PE...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7060116
更新日期：2018-05-23 00:00:00
abstract：:Maternal and pediatric delivery outcomes may vary in patients who underwent open fetal myelomeningocele repair and elected to deliver at the fetal center where their fetal intervention was performed versus at the referring physician's hospital. A prospective cohort study of 88 patients were evaluated following in uter...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113443
更新日期：2020-10-27 00:00:00
abstract：:The purpose of this study was to report on the clinical usefulness of the Liver Disease Quality of Life Instrument (LDQOL) 1.0, which was prospectively measured in chronic hepatitis B patients. We regularly followed up with patients with chronic hepatitis B between 2008 and 2010 who were enrolled in the study, and the...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8050656
更新日期：2019-05-10 00:00:00
abstract：:Endometrial cancer (EC) is the sixth most common cancer in women worldwide and its mortality is directly associated with the presence of poor prognostic factors driving tumor recurrence. Stratification systems are based on few molecular, and mostly clinical and pathological parameters, but these systems remain inaccur...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9061900
更新日期：2020-06-17 00:00:00
