Abstract：
:Tumor cells exhibit phenotypic and genotypic differences in comparison to normal cells. These differences can be used to identify proteins important for tumor growth and, therefore, potentially used in the diagnosis and follow-up of patients. The objective of this work was to investigate the electrophoretic pattern of cytoplasm membrane proteins from normal and malignant cervix using polyacrylamide-SDS gels. A highly reproducible protein pattern was found in the 29 samples of normal cervix whereas three well-defined patterns of protein bands were observed in the 48 tumor specimens (pattern I: 25%, pattern II: 29.2% and pattern III: 45.8%). A low concentration or absence of high molecular weight proteins was observed (p<0.5) in tumor samples. None of the tumor protein patterns correlated with the clinicopathologic characteristics of patients. Nine out of 11 patients (82%) showing the pattern III had a complete clinical response whereas only 55% (11 out of 20) of those with patterns I and II showed a complete response. However, this difference was non-significant (p=0.1247). In conclusion, we demonstrate that there is a gain and loss of cytoplasmic membrane proteins in tumors, shown as different protein band patterns. These findings could have clinical and biological significance that must be further evaluated.
journal_name
J Exp Clin Cancer Resjournal_title
Journal of experimental & clinical cancer research : CRauthors
Trejo-Becerril C,Pérez-Cárdenas E,Taja-Chayeb L,Zeichner-Gancz I,Delgado-Chávez R,Solorza-Luna G,Dueñas-González Akeywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
231-7issue
2eissn
0392-9078issn
1756-9966journal_volume
20pub_type
杂志文章abstract：BACKGROUND:In this research, we aimed to resolve contradictory results whether SOX9 plays a positive or negative role in melanoma progression and determine whether SOX9 and its closely related member SOX10 share the same or distinct targets in mediating their functions in melanoma. METHODS:Immunofluorescence, TCGA dat...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-018-0998-6
更新日期：2019-01-14 00:00:00
abstract：:Preclinical research and ongoing clinical trials are validating epidermal growth factor receptor (EGFR) as a suitable molecular target in cancer therapy. Here we report the case of a 39-year-old non-smoking man with heavily pretreated stage IV bronchioloalveolar NSCLC who was treated with gefitinib ('Iressa', ZD1839) ...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:In the deletion mapping of chromosome (chr) 9 in head and neck lesions of the Indian patient population by microsatellite markers, we have identified four discrete areas (D1-D4) with high loss of heterozygosities (LOHs) viz. 9p24-p23 (D1), 9p22-p21 (D2), 9q11-q13 (D3) and 9q22.3 (D4) regions. The deletions in D2 and D...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 历史文章,杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:This study was conducted to establish a feasible intraperitoneal xenograft model in nude mice which mimicked the dynamic progression of human ovarian cancer and to explore its potential for preclinical trials. A human ovarian tumor line SKOV3 was originally injected s.c. to develop tumors; then the tumors were harvest...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND:Immunocytochemistry and RT-PCR have been widely used for the detection of circulating tumor cells in patients with breast cancer but their specificity is limited. Our purpose is to utilize a convenient and specific technology to detect circulating tumor cells in breast cancer patients. METHODS:To determine ...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/1756-9966-28-57
更新日期：2009-04-28 00:00:00
abstract：BACKGROUND:MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors. METHODS:To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miR...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/1756-9966-28-82
更新日期：2009-06-16 00:00:00
abstract：BACKGROUND:Growing evidences indicate that circular RNAs (circRNAs) play an important role in the regulation of biological behavior of tumor. We aim to explore the role of circRNA in glioma and elucidate how circRNA acts. METHODS:Real-time PCR was used to examine the expression of circPTN in glioma tissues and normal ...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-019-1376-8
更新日期：2019-09-11 00:00:00
abstract：:In the present study we report twenty-nine patients with iatrogenic injuries and management during various operations for malignant conditions. The patients were reviewed in order to identify and study the incidence, type of treatment administered and outcome. The study group was composed of 29 patients with 31 iatrog...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND:The evasion of cell death is one of the hallmarks of cancer, contributing to both tumor progression and resistance to therapy. Dedifferentiated and anaplastic thyroid carcinomas that do not take up radioiodine are resistant to conventional anticancer treatments and patients with these tumors are difficult to...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-015-0186-x
更新日期：2015-07-22 00:00:00
abstract：BACKGROUND:Measurement of prostate-specific antigen (PSA) advanced the diagnostic and prognostic potential for prostate cancer (PCa). However, due to PSA's lack of specificity, novel biomarkers are needed to improve risk assessment and ensure optimal personalized therapy. A set of protein molecules as potential biomark...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-015-0161-6
更新日期：2015-05-14 00:00:00
abstract：BACKGROUND:Cancer stem cells (CSCs) are important in the tumorigenesis and progression of hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) play crucial roles regulating CD133+ and EpCAM+ CSCs in HCC, although it is unclear whether miRNAs regulate CD90+ CSCs in HCC. METHODS:The miRNA profiles of CD90+ and CD90- HCC c...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-016-0452-6
更新日期：2016-11-11 00:00:00
abstract：:Insulin-secreting tumors are the commonest hormone-producing neoplasm of the gastrointestinal tract. They occur with an incidence of 4 cases per million per year. About 10% of them are metastatic and malignant insulinomas very rarely observed in children and in elderly. We report a rare case of very large malignant in...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章,评审
doi：
更新日期：2007-12-01 00:00:00
abstract：:The aim of the current study was to evaluate the activity and toxicity of a combination of oxaliplatin with bolus fluorouracil and leucovorin in colorectal cancer (CRC) patients pretreated for advanced disease with various schedules including continuous fluorouracil infusion. Thirty consecutive patients with pretreate...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:This study aimed to analyze three-dimensional (3D) dosimetric data of conventional two-dimensional (2D) palliative spinal bone irradiation using different reference points and treatment plans with respect to the International Commission on Radiation Units and Measurements (ICRU) Report 50. METHODS:Forty-fiv...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/1756-9966-28-2
更新日期：2009-01-07 00:00:00
abstract：BACKGROUND:Celastrol, a triterpene compound derived from the traditional Chinese medicine Tripterygium wilfordii, has been reported to possess potential antitumor activity towards various malignancies. However, the effect of celastrol on glioma cells and the underlying molecular mechanisms remain elusive. METHODS:Glio...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-019-1173-4
更新日期：2019-05-03 00:00:00
abstract：BACKGROUND:Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. MATERIALS AND METHODS:qRT-PCR and western blot were conducted to measure relev...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-020-01551-9
更新日期：2020-04-09 00:00:00
abstract：:The rational making the G protein-coupled receptors (GPCR) the centerpiece of targeted therapies is fueled by the awareness that GPCR-initiated signaling acts as pivotal driver of the early stages of progression in a broad landscape of human malignancies. The endothelin-1 (ET-1) receptors (ET-1R), known as ETA recepto...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章,评审
doi：10.1186/s13046-021-01827-8
更新日期：2021-01-09 00:00:00
abstract：:Axillary lymphadenectomy is a very important procedure in the staging of breast cancer patients. However, it is associated with a significant morbidity rate. On the other hand, using early diagnosis we can see a high number of cases where the lymph nodes are negatives. With the intention of avoiding unnecessary axilla...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:The present study assesses the adaptation of a group of female patients with either manifest or suspected breast cancer who have undergone Magnetic Resonance Imaging (MRI) after receiving psychological support. Several studies in literature have reported the effectiveness of such support in reducing anti-oxidant and d...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:Renal cell carcinoma, in a high percentage of patients, metastasizes early, sometimes mimicking other lesions. We present a case of an asymptomatic papillary renal cell carcinoma that presented neck metastases as the initial manifestation. The laterocervical and supraclavicular masses were considered consistent with n...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：BACKGROUND:This is a retrospective cross sectional study aimed to verify whether Multiplex Ligation-dependent Probe Amplification (MLPA), a quantitative molecular assay, may represent a valuable reflex test in breast cancer with equivocal HER2 expression by immunohistochemistry and HER2 gene signals/nucleus (s/n) rangi...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-017-0613-2
更新日期：2017-10-13 00:00:00
abstract：BACKGROUND:Studying the intracellular distribution of pharmacological agents, including anticancer compounds, is of central importance in biomedical research. It constitutes a prerequisite for a better understanding of the molecular mechanisms underlying drug action and resistance development. Hyperactivated fibroblast...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-017-0592-3
更新日期：2017-09-07 00:00:00
abstract：BACKGROUND:Statins are inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, the rate-limiting enzyme in cholesterol synthesis. The inhibition of this key enzyme in the mevalonate pathway leads to suppression of cell proliferation and induction of apoptosis. However, the molecular mechanism of apoptosis induct...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/1756-9966-30-74
更新日期：2011-08-10 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via the original article. ...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章,撤回出版物
doi：10.1186/s13046-020-01668-x
更新日期：2020-08-19 00:00:00
abstract：:The aim of this study was to investigate serum levels of IL-2, IL-6 and IL-10 in the pretreatment period and to determine if high IL-2, IL-6, IL-10 levels correlate with the outcome in patients with Non-Hodgkin's Lymphoma (NHL) in the post treatment period. Forty-three patients with the diagnosis of aggressive NHL wer...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Intraoperative radiotherapy with a single dose of electrons (ELIOT) in the conservative treatment of breast cancer is a possibility under evaluation in clinical trials. The costs of the mobile linear accelerator with a robotic arm, used in intraoperative radiotherapy, are prohibitive for poor countries. The aim of thi...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:Our previous studies have demonstrated the significant enzymatic activity of glycogen phosphorylase (GP) in the gastric carcinoma and proliferating cells of particular intestinal metaplasia (IM). This paper reviewed the identification of the GP isoform in the gastrointestinal carcinoma, and the investigation on the ro...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章,评审
doi：
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:Annexin A2 is increased in serous ovarian cancer and plays an essential role in ovarian cancer invasion and metastasis. In combination with S100A10, annexin A2 plays an important role in the plasminogen activator system regulating plasmin production. The aim of this study was to investigate the potential uti...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-018-1017-7
更新日期：2019-01-08 00:00:00
abstract：:Tumor ablation by radiofrequency (RFA) is an appealing therapeutical strategy for the treatment of liver tumors (hepatocarcinoma and metastatic lesions) to be used as valid alternative to the surgical resection that often is appropriate and feasible in only a minority of patients. RFA induces the localised and control...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：BACKGROUND:Triple negative breast cancer (TNBC) is an aggressive neoplasia with no effective therapy. Our laboratory has developed a unique TNBC cell model presenting epithelial mesenchymal transition (EMT) a process known to be important for tumor progression and metastasis. There is increasing evidence showing that e...
journal_title：Journal of experimental & clinical cancer research : CR
pub_type： 杂志文章
doi：10.1186/s13046-018-0988-8
更新日期：2018-12-14 00:00:00
