Activated T cells induce up-regulation of Fas antigen in cultured endothelial cells.

Abstract:

:Recent evidence has shown that apoptotic cells are present in human atherosclerotic lesions. However, the molecular mechanism of the induction of apoptosis in atherosclerotic lesions is not clear. Since T cells are present in almost all stages of atherosclerosis, we examined whether T cells can modulate the expression of Fas, a death signal, in endothelial cells (ECs), using a coculture system. Human umbilical vein ECs were cultured alone or cocultured with human peripheral T cells activated with phorbol myristate acetate and ionomycin. Flow cytometric (FACscan) analysis showed that Fas antigen was up-regulated in ECs when ECs were cocultured for 24 h with activated T cells. However, Fas antigen was not up-regulated in ECs cocultured with non-activated T cells. The up-regulation of Fas antigen induced by coculturing ECs with activated T cells was partially, but significantly, neutralized by antibody against interferon-gamma (IFN-gamma). Actually, incubation with IFN-gamma induced a dose-dependent increase in the level of Fas antigen in ECs cultured alone. These findings indicate that activated T cells induce up-regulation of Fas antigen in ECs. Thus, the Fas system induced by activated T cells could participate in the mechanism of EC injury in atherosclerotic lesions.

journal_name

Heart Vessels

journal_title

Heart and vessels

authors

Fukuo K,Suhara T,Nakahashi T,Hata S,Shimizu M,Niinobu T,Morimoto S,Ogihara T

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

81-3

eissn

0910-8327

issn

1615-2573

journal_volume

Suppl 12

pub_type

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